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one step prime scripttm rt pcr kit  (TaKaRa)


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    TaKaRa one step prime scripttm rt pcr kit
    One Step Prime Scripttm Rt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 460 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptase+prime+scripttm+rt/Titanium+One-Step+RT-PCR+Kit/pm36010275-54-8-13
    Average 96 stars, based on 460 article reviews
    one step prime scripttm rt pcr kit - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    One Step RT-PCR:



    Article Title: Wound healing improvement by a multicomponent wound dressing of keratinocyte-imprinted polydimethylsiloxane substrate in a rabbit model
    Article Snippet: To analyze the expressions of keratinocyte-specific genes in differentiated ADSCs by quantitative real time-PCR (qPCR), total RNAs were extracted after 14 days using FavorPrep Blood/Cultured Cell Total RNA Mini Kit (FAVORGEN, Taiwan) according to the manufacturer’s instruction. .. Then the ratios of 260/230 and 260/280 were detected using an Epoch spectrophotometer (BioTek, USA), and cDNA was synthesized using One-Step RT-PCR Kit (TaKaRa, Japan). .. Finally, qPCR was performed using the StepOne equipment (Applied Biosystems, USA) and SYBR PCR Master Mix (Takara, Japan) for rabbit involucrin, collagen type I, cytokeratin10 as target genes, and GAPDH as reference gene.

    Article Title: Hyperosmolarity-Induced Oxidative Stress Leads to Senescence in Human Corneal Epithelial Cells (HCEPC) via DNA Damage, Metabolic Disturbance and Mitophagy Decline
    Article Snippet: Total RNA was extracted from HCEPCs using a total RNA extraction kit (DP419, Tiangen, Beijing, China). .. RT-qPCR was subsequently performed using the One Step RT-PCR Kit (RR066A, Takara, Shiga, Japan) on a Roche Light Cycler 480 Real-Time PCR System (Roche, Basel, Switzerland), in accordance with the manufacturers’ standard protocols. ..

    Article Title: Wound healing improvement by a multicomponent wound dressing of keratinocyte-imprinted polydimethylsiloxane substrate in a rabbit model.
    Article Snippet: To analyze the expressions of keratinocyte-specific genes in differentiated ADSCs by quantitative real time-PCR (qPCR), total RNAs were extracted after 14 days using FavorPrep Blood/Cultured Cell Total RNA Mini Kit (FAVORGEN, Taiwan) according to the manufacturer’s instruction. .. Then the ratios of 260/230 and 260/280 were detected using an Epoch spectrophotometer (BioTek, USA), and cDNA was synthesized using One-Step RT-PCR Kit Scientific Reports | (2025) 15:42487 11| https://doi.org/10.1038/s41598-025-26525-9 (TaKaRa, Japan). .. Finally, qPCR was performed using the StepOne equipment (Applied Biosystems, USA) and SYBR PCR Master Mix (Takara, Japan) for rabbit involucrin, collagen type I, cytokeratin10 as target genes, and GAPDH as reference gene.

    Article Title: From Cells to Animals: Connexin43 Suppression Enhances Autophagic Flux to Restore Odontogenesis in Inflamed Dental Pulp.
    Article Snippet: Total RNA was extracted from cultured cells and first- strand cDNA was synthesised from 0.5 μg of RNA using an Advantage RT- for- PCR Kit (Takara Bio Inc., JP). .. Gene expression was analysed by real- time quantitative polymerase chain reaction (RT- qPCR) with an Applied Biosystems (Thermo Fisher) 7500 Real- time PCR System in 20 μL of Titanium One- Step RT- PCR Kit (Takara) containing 10 mmol/L each of the specific primers (Table 1). ..

    Spectrophotometry:

    Article Title: Wound healing improvement by a multicomponent wound dressing of keratinocyte-imprinted polydimethylsiloxane substrate in a rabbit model
    Article Snippet: To analyze the expressions of keratinocyte-specific genes in differentiated ADSCs by quantitative real time-PCR (qPCR), total RNAs were extracted after 14 days using FavorPrep Blood/Cultured Cell Total RNA Mini Kit (FAVORGEN, Taiwan) according to the manufacturer’s instruction. .. Then the ratios of 260/230 and 260/280 were detected using an Epoch spectrophotometer (BioTek, USA), and cDNA was synthesized using One-Step RT-PCR Kit (TaKaRa, Japan). .. Finally, qPCR was performed using the StepOne equipment (Applied Biosystems, USA) and SYBR PCR Master Mix (Takara, Japan) for rabbit involucrin, collagen type I, cytokeratin10 as target genes, and GAPDH as reference gene.

    Article Title: Wound healing improvement by a multicomponent wound dressing of keratinocyte-imprinted polydimethylsiloxane substrate in a rabbit model.
    Article Snippet: To analyze the expressions of keratinocyte-specific genes in differentiated ADSCs by quantitative real time-PCR (qPCR), total RNAs were extracted after 14 days using FavorPrep Blood/Cultured Cell Total RNA Mini Kit (FAVORGEN, Taiwan) according to the manufacturer’s instruction. .. Then the ratios of 260/230 and 260/280 were detected using an Epoch spectrophotometer (BioTek, USA), and cDNA was synthesized using One-Step RT-PCR Kit Scientific Reports | (2025) 15:42487 11| https://doi.org/10.1038/s41598-025-26525-9 (TaKaRa, Japan). .. Finally, qPCR was performed using the StepOne equipment (Applied Biosystems, USA) and SYBR PCR Master Mix (Takara, Japan) for rabbit involucrin, collagen type I, cytokeratin10 as target genes, and GAPDH as reference gene.

    Synthesized:

    Article Title: Wound healing improvement by a multicomponent wound dressing of keratinocyte-imprinted polydimethylsiloxane substrate in a rabbit model
    Article Snippet: To analyze the expressions of keratinocyte-specific genes in differentiated ADSCs by quantitative real time-PCR (qPCR), total RNAs were extracted after 14 days using FavorPrep Blood/Cultured Cell Total RNA Mini Kit (FAVORGEN, Taiwan) according to the manufacturer’s instruction. .. Then the ratios of 260/230 and 260/280 were detected using an Epoch spectrophotometer (BioTek, USA), and cDNA was synthesized using One-Step RT-PCR Kit (TaKaRa, Japan). .. Finally, qPCR was performed using the StepOne equipment (Applied Biosystems, USA) and SYBR PCR Master Mix (Takara, Japan) for rabbit involucrin, collagen type I, cytokeratin10 as target genes, and GAPDH as reference gene.

    Article Title: Wound healing improvement by a multicomponent wound dressing of keratinocyte-imprinted polydimethylsiloxane substrate in a rabbit model.
    Article Snippet: To analyze the expressions of keratinocyte-specific genes in differentiated ADSCs by quantitative real time-PCR (qPCR), total RNAs were extracted after 14 days using FavorPrep Blood/Cultured Cell Total RNA Mini Kit (FAVORGEN, Taiwan) according to the manufacturer’s instruction. .. Then the ratios of 260/230 and 260/280 were detected using an Epoch spectrophotometer (BioTek, USA), and cDNA was synthesized using One-Step RT-PCR Kit Scientific Reports | (2025) 15:42487 11| https://doi.org/10.1038/s41598-025-26525-9 (TaKaRa, Japan). .. Finally, qPCR was performed using the StepOne equipment (Applied Biosystems, USA) and SYBR PCR Master Mix (Takara, Japan) for rabbit involucrin, collagen type I, cytokeratin10 as target genes, and GAPDH as reference gene.

    Quantitative RT-PCR:

    Article Title: Hyperosmolarity-Induced Oxidative Stress Leads to Senescence in Human Corneal Epithelial Cells (HCEPC) via DNA Damage, Metabolic Disturbance and Mitophagy Decline
    Article Snippet: Total RNA was extracted from HCEPCs using a total RNA extraction kit (DP419, Tiangen, Beijing, China). .. RT-qPCR was subsequently performed using the One Step RT-PCR Kit (RR066A, Takara, Shiga, Japan) on a Roche Light Cycler 480 Real-Time PCR System (Roche, Basel, Switzerland), in accordance with the manufacturers’ standard protocols. ..

    Article Title: Ubiquitin-Conjugating Enzyme Positively Regulates Salicylic Acid and Jasmonic Acid Biosynthesis to Confer Broad-Spectrum Antiviral Resistance in Nicotiana benthamiana
    Article Snippet: We extracted total RNA from N. benthamiana leaves using TRIzol reagent and treated the RNA with RNase-free DNase I (TransGen, Beijing, China) to remove potential DNA contamination. .. We performed first-strand cDNA synthesis with oligo(dT)12-18 primers using a Titanium One-Step RT-qPCR Kit (TaKaRa, Tokyo, Japan). ..

    Article Title: Ubiquitin-Conjugating Enzyme Positively Regulates Salicylic Acid and Jasmonic Acid Biosynthesis to Confer Broad-Spectrum Antiviral Resistance in Nicotiana benthamiana .
    Article Snippet: We extracted total RNA from N. benthamiana leaves using TRIzol reagent and treated the RNA with RNase-free DNase I (TransGen, Beijing, China) to remove potential DNA contamination. .. We performed first-strand cDNA synthesis with oligo(dT)12-18 primers using a Titanium One-Step RT-qPCR Kit (TaKaRa, Tokyo, Japan). ..

    Article Title: From Cells to Animals: Connexin43 Suppression Enhances Autophagic Flux to Restore Odontogenesis in Inflamed Dental Pulp.
    Article Snippet: Total RNA was extracted from cultured cells and first- strand cDNA was synthesised from 0.5 μg of RNA using an Advantage RT- for- PCR Kit (Takara Bio Inc., JP). .. Gene expression was analysed by real- time quantitative polymerase chain reaction (RT- qPCR) with an Applied Biosystems (Thermo Fisher) 7500 Real- time PCR System in 20 μL of Titanium One- Step RT- PCR Kit (Takara) containing 10 mmol/L each of the specific primers (Table 1). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Hyperosmolarity-Induced Oxidative Stress Leads to Senescence in Human Corneal Epithelial Cells (HCEPC) via DNA Damage, Metabolic Disturbance and Mitophagy Decline
    Article Snippet: Total RNA was extracted from HCEPCs using a total RNA extraction kit (DP419, Tiangen, Beijing, China). .. RT-qPCR was subsequently performed using the One Step RT-PCR Kit (RR066A, Takara, Shiga, Japan) on a Roche Light Cycler 480 Real-Time PCR System (Roche, Basel, Switzerland), in accordance with the manufacturers’ standard protocols. ..

    Article Title: From Cells to Animals: Connexin43 Suppression Enhances Autophagic Flux to Restore Odontogenesis in Inflamed Dental Pulp.
    Article Snippet: Total RNA was extracted from cultured cells and first- strand cDNA was synthesised from 0.5 μg of RNA using an Advantage RT- for- PCR Kit (Takara Bio Inc., JP). .. Gene expression was analysed by real- time quantitative polymerase chain reaction (RT- qPCR) with an Applied Biosystems (Thermo Fisher) 7500 Real- time PCR System in 20 μL of Titanium One- Step RT- PCR Kit (Takara) containing 10 mmol/L each of the specific primers (Table 1). ..

    cDNA Synthesis:

    Article Title: Ubiquitin-Conjugating Enzyme Positively Regulates Salicylic Acid and Jasmonic Acid Biosynthesis to Confer Broad-Spectrum Antiviral Resistance in Nicotiana benthamiana
    Article Snippet: We extracted total RNA from N. benthamiana leaves using TRIzol reagent and treated the RNA with RNase-free DNase I (TransGen, Beijing, China) to remove potential DNA contamination. .. We performed first-strand cDNA synthesis with oligo(dT)12-18 primers using a Titanium One-Step RT-qPCR Kit (TaKaRa, Tokyo, Japan). ..

    Article Title: Ubiquitin-Conjugating Enzyme Positively Regulates Salicylic Acid and Jasmonic Acid Biosynthesis to Confer Broad-Spectrum Antiviral Resistance in Nicotiana benthamiana .
    Article Snippet: We extracted total RNA from N. benthamiana leaves using TRIzol reagent and treated the RNA with RNase-free DNase I (TransGen, Beijing, China) to remove potential DNA contamination. .. We performed first-strand cDNA synthesis with oligo(dT)12-18 primers using a Titanium One-Step RT-qPCR Kit (TaKaRa, Tokyo, Japan). ..

    Gene Expression:

    Article Title: From Cells to Animals: Connexin43 Suppression Enhances Autophagic Flux to Restore Odontogenesis in Inflamed Dental Pulp.
    Article Snippet: Total RNA was extracted from cultured cells and first- strand cDNA was synthesised from 0.5 μg of RNA using an Advantage RT- for- PCR Kit (Takara Bio Inc., JP). .. Gene expression was analysed by real- time quantitative polymerase chain reaction (RT- qPCR) with an Applied Biosystems (Thermo Fisher) 7500 Real- time PCR System in 20 μL of Titanium One- Step RT- PCR Kit (Takara) containing 10 mmol/L each of the specific primers (Table 1). ..



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