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one step sybr prime scripttm plus rt pcr kit  (TaKaRa)


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    Structured Review

    TaKaRa one step sybr prime scripttm plus rt pcr kit
    One Step Sybr Prime Scripttm Plus Rt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 13586 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptase+prime+scripttm+rt/PCR+Amplification+Kit/pmc05020468-93-22-30
    Average 96 stars, based on 13586 article reviews
    one step sybr prime scripttm plus rt pcr kit - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: Viral Contaminants in a Philippine Wastewater Treatment Plant: Quantification and Treatment Reduction
    Article Snippet: .. The thermal conditions for Inf-A, SARS-CoV-2, PMMoV, and Pseudomonas bacteriophage Φ6 were at 25 °C for 10 min, 52 °C for 5 min, and 95 °C for 10 s, followed by 45 cycles at 95 °C for 5 s and 60 °C for 30 s. The Norovirus Detection RT-qPCR Kit for Wastewater (Takara Bio) was used to quantify NoV-GI and NoV-GII by preparing a 25-μL mixture consisting of 10.0 μL of RT-qPCR buffer, 2.0 μL of Enzyme Mix, 1.0 μL of NV (GI/GII) Primer/Probe (Cy5 for GI and FAM for GII), 7.0 μL of RNase-free water, and 5 μL of the extracted nucleic acid. .. The thermal cyclic parameters were set as follows: 25 °C for 10 min, 42 °C for 5 min, and 95 °C for 30 s, followed by 45 cycles of 95 °C for 5 s and 56 °C for 30 s. Standard curves were generated for each viral target by preparing six 10-fold serial dilutions of the synthesized plasmid DNA for crAssphage (10 6 copies/5μL; containing the 125-bp amplification region sequences of 5’-CAGAAGTACAAACTCCTAAAAAACGTAGAGGTAGAGGTATTAATAACGATTTACGTGATGTAACTCGTAAAAAGTTTGATGAACGTACTGATTGTAATAAAGCTAATGGCTTGTTTATTGGTCAT-3’) and Inf-A (10 6 copies/5μL; containing the 146-bp amplification region sequences of 5’-CCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAAAGTGTCTTTGCAGGAAAGAACACAGATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCTTGTCA-3’) (Eurofins, Tokyo, Japan), positive control DNA of SARS-CoV-2 and PMMoV/Φ6 (10 6 copies/μL) in the SARS-CoV-2 Detection RT-qPCR Kit for Wastewater for SARS-CoV-2, PMMoV, and Φ6, and positive control DNA of NoV (10 6 copies/μL) in the Norovirus Detection RT-qPCR Kit for Wastewater for NoV-GI and NoV-GII.

    Article Title: Viral Contaminants in a Philippine Wastewater Treatment Plant: Quantification and Treatment Reduction.
    Article Snippet: .. The thermal conditions for Inf-A, SARS-CoV-2, PMMoV, and Pseudomonas bacteriophage Φ6 were at 25 °C for 10 min, 52 °C for 5 min, and 95 °C for 10 s, followed by 45 cycles at 95 °C for 5 s and 60 °C for 30 s. The Norovirus Detection RT-qPCR Kit for Wastewater (Takara Bio) was used to quantify NoV-GI and NoV-GII by preparing a 25-μL mixture consisting of 10.0 μL of RT-qPCR buffer, 2.0 μL of Enzyme Mix, 1.0 μL of NV (GI/GII) Primer/Probe (Cy5 for GI and FAM for GII), 7.0 μL of RNase-free water, and 5 μL of the extracted nucleic acid. .. The thermal cyclic parameters were set as follows: 25 °C for 10 min, 42 °C for 5 min, and 95 °C for 30 s, followed by 45 cycles of 95 °C for 5 s and 56 °C for 30 s. Standard curves were generated for each viral target by preparing six 10-fold serial dilutions of the synthesized plasmid DNA for crAssphage (106 copies/5μL; containing the 125-bp amplification region sequences of 5’- C A G A A G T A C A A A C T C C T A A A A A A C G T A G A G G T A G A G G T A T T A A T A A C G A T T T A C G T G A T G T A A C T C G T A A A A A G T T T G A T G A A C G T A C T G A T T G T A A T A A A G C T A A T G G C T T G T T T A T T G G T C A T-3’) and Inf-A (106 copies/5μL; containing the 146-bp amplification region sequences of 5’- C C G A G G T C G A A A C G T A C G T T C T T T C T A T C A T C C C G T C A G G C C C C C T C A A A G C C G A G A T C G C G C A G A G A C T G G A A A G T G T C T T T G C A G G A A A G A A C A C A G A T C T T G A G G C T C T C A T G G A A T G G C T A A A G A C A A G A C C A A T C T T G T C A-3’) (Eurofins, Tokyo, Japan), positive control DNA of SARS-CoV-2 and PMMoV/Φ6 (106 copies/μL) in the SARS-CoV-2 Detection RT-qPCR Kit for Wastewater for SARS-CoV-2, PMMoV, and Φ6, and positive control DNA of NoV (106 copies/μL) in the Norovirus Detection RT-qPCR Kit for Wastewater for NoV-GI and NoV-GII.

    Polymerase Chain Reaction:

    Article Title: Cold-brewed hawthorn juice ameliorates high-fat diet-induced obesity in mice by regulating gut microbiota and lipid metabolism
    Article Snippet: .. PCR amplification of the bacterial 16S rRNA gene was performed with barcoded primers and Takara ExTaq high-fidelity polymerase. ..

    Article Title: Electroacupuncture alleviates ferroptosis through miR-145-5p/ACSL4 axis to reduce post-reperfusion brain injury in ischemic stroke.
    Article Snippet: To synthesize RNA into cDNA, AMV reverse transcriptase (2621, TAKARA, Tokyo, Japan) was utilized, which provided a template for subsequent PCR amplification. .. Subsequently, PCR amplification of the target gene was performed through the TB Green FAST qPCR Kit (CN830S, TAKARA). ..

    Article Title: T cell receptors and methods of use thereof
    Article Snippet: Genomic DNA of gp100 was isolated from SK-MEL-28 using PureLink Genomic DNA Mini Kit (Invitrogen). .. TCR genes were cloned by 5′-rapid amplification of cDNA ends (RACE) PCR using a SMARTer RACE cDNA amplification kit (Takara Bio). ..

    Article Title: Isolation and Identification of G8P[1] Bovine Rotavirus A Among Neonatal Diarrheic Calves in Yunnan, China
    Article Snippet: .. Reverse transcription polymerase chain reaction (RT-PCR) was performed using the PrimeScriptTM One-Step RT-PCR Kit (TaKaRa, Dalian, China). ..

    Amplification:

    Article Title: Cold-brewed hawthorn juice ameliorates high-fat diet-induced obesity in mice by regulating gut microbiota and lipid metabolism
    Article Snippet: .. PCR amplification of the bacterial 16S rRNA gene was performed with barcoded primers and Takara ExTaq high-fidelity polymerase. ..

    Article Title: Electroacupuncture alleviates ferroptosis through miR-145-5p/ACSL4 axis to reduce post-reperfusion brain injury in ischemic stroke.
    Article Snippet: To synthesize RNA into cDNA, AMV reverse transcriptase (2621, TAKARA, Tokyo, Japan) was utilized, which provided a template for subsequent PCR amplification. .. Subsequently, PCR amplification of the target gene was performed through the TB Green FAST qPCR Kit (CN830S, TAKARA). ..

    Article Title: T cell receptors and methods of use thereof
    Article Snippet: Genomic DNA of gp100 was isolated from SK-MEL-28 using PureLink Genomic DNA Mini Kit (Invitrogen). .. TCR genes were cloned by 5′-rapid amplification of cDNA ends (RACE) PCR using a SMARTer RACE cDNA amplification kit (Takara Bio). ..

    Article Title: Pachymic Acid Alleviates Non‐Alcoholic Fatty Liver Disease via FGF21 ‐Mediated Inhibition of the p38 MAPK Pathway
    Article Snippet: .. RNA was then reverse‐transcribed into cDNA with a Takara reverse transcription kit, followed by amplification and quantification by qRT‐PCR. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Electroacupuncture alleviates ferroptosis through miR-145-5p/ACSL4 axis to reduce post-reperfusion brain injury in ischemic stroke.
    Article Snippet: To synthesize RNA into cDNA, AMV reverse transcriptase (2621, TAKARA, Tokyo, Japan) was utilized, which provided a template for subsequent PCR amplification. .. Subsequently, PCR amplification of the target gene was performed through the TB Green FAST qPCR Kit (CN830S, TAKARA). ..

    Clone Assay:

    Article Title: T cell receptors and methods of use thereof
    Article Snippet: Genomic DNA of gp100 was isolated from SK-MEL-28 using PureLink Genomic DNA Mini Kit (Invitrogen). .. TCR genes were cloned by 5′-rapid amplification of cDNA ends (RACE) PCR using a SMARTer RACE cDNA amplification kit (Takara Bio). ..

    Reverse Transcription:

    Article Title: Isolation and Identification of G8P[1] Bovine Rotavirus A Among Neonatal Diarrheic Calves in Yunnan, China
    Article Snippet: .. Reverse transcription polymerase chain reaction (RT-PCR) was performed using the PrimeScriptTM One-Step RT-PCR Kit (TaKaRa, Dalian, China). ..

    Article Title: Pachymic Acid Alleviates Non‐Alcoholic Fatty Liver Disease via FGF21 ‐Mediated Inhibition of the p38 MAPK Pathway
    Article Snippet: .. RNA was then reverse‐transcribed into cDNA with a Takara reverse transcription kit, followed by amplification and quantification by qRT‐PCR. ..

    One Step RT-PCR:

    Article Title: Isolation and Identification of G8P[1] Bovine Rotavirus A Among Neonatal Diarrheic Calves in Yunnan, China
    Article Snippet: .. Reverse transcription polymerase chain reaction (RT-PCR) was performed using the PrimeScriptTM One-Step RT-PCR Kit (TaKaRa, Dalian, China). ..



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