Review




Structured Review

Epicentre Technologies Corp mmlv high-performance reverse transcriptase
Mmlv High Performance Reverse Transcriptase, supplied by Epicentre Technologies Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transcriptase+enzyme/mmlv+high+performance+reverse+transcriptase+enzyme/pm38520036-112-59-63
Average 90 stars, based on 1 article reviews
mmlv high-performance reverse transcriptase - by Bioz Stars, 2026-11
90/100 stars

Images

Related Articles

Extraction:

Article Title: Oxygen tension during in vitro oocyte maturation and fertilization affects embryo quality in sheep and deer.
Article Snippet: Incubation gas atmosphere affects the development of in vitro produced embryos.. In this study, there was examination of effects of two different oxygen (O2) tensions (5 % and 21 %) during in vitro maturation (M5 and M21) and/or fertilization (F5 and F21) on embryo production and quality in deer and sheep.. There was assessment of the percentage of embryos with cell cleavage occurring, percentage that developed to the blastocyst stage, and analysis of the relative abundance of mRNA transcript for genes important for development to the blastocyst stage.

Article Title: Hepatoma-derived growth factor: Protein quantification in uterine fluid, gene expression in endometrial-cell culture and effects on in vitro embryo development, pregnancy and birth.
Article Snippet: Hepatoma-derived growth factor (HDGF) is present in the endometrium of cows and other mammals.. Recombinant HDGF (rHDGF) improves bovine blastocyst development in vitro.. However, specific culture conditions and essential aspects of HDGF uterine physiology are yet unknown.

Article Title: Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2
Article Snippet: .. After extraction, RT reaction was carried out following the manufacturer’s instructions (Epicentre Technologies Corp, Madison, WI, USA) using poly(T) primers, random primers and MMLV High Performance Reverse Transcriptase enzyme in a total volume of 40 μL, to prime the RT reaction and produce cDNA. ..

Article Title: Resveratrol-cyclodextrin complex affects the expression of genes associated with lipid metabolism in bovine in vitro produced embryos.
Article Snippet: In vitro fertilization (IVF), manipulation of gametes and embryos and in vitro culture conditions expose cells to environmental factors that may affect antioxidant defence mechanism and therefore, negatively impacting the oocyte and the subsequent embryo, compared with those produced in vivo (Agarwal, Durairajanayagam, & du Plessis, 2014).. To improve the quality of the oocyte and the developmental capacities of embryos cultured in vitro, antioxidant compounds have been used to supplement the culture medium.. One of the most important polyphenols found in red wine is resveratrol (3,4,5trihydroxytransstilbene).

Reverse Transcription:

Article Title: Oxygen tension during in vitro oocyte maturation and fertilization affects embryo quality in sheep and deer.
Article Snippet: Incubation gas atmosphere affects the development of in vitro produced embryos.. In this study, there was examination of effects of two different oxygen (O2) tensions (5 % and 21 %) during in vitro maturation (M5 and M21) and/or fertilization (F5 and F21) on embryo production and quality in deer and sheep.. There was assessment of the percentage of embryos with cell cleavage occurring, percentage that developed to the blastocyst stage, and analysis of the relative abundance of mRNA transcript for genes important for development to the blastocyst stage.

Article Title: Hepatoma-derived growth factor: Protein quantification in uterine fluid, gene expression in endometrial-cell culture and effects on in vitro embryo development, pregnancy and birth.
Article Snippet: Hepatoma-derived growth factor (HDGF) is present in the endometrium of cows and other mammals.. Recombinant HDGF (rHDGF) improves bovine blastocyst development in vitro.. However, specific culture conditions and essential aspects of HDGF uterine physiology are yet unknown.

Article Title: Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2
Article Snippet: .. After extraction, RT reaction was carried out following the manufacturer’s instructions (Epicentre Technologies Corp, Madison, WI, USA) using poly(T) primers, random primers and MMLV High Performance Reverse Transcriptase enzyme in a total volume of 40 μL, to prime the RT reaction and produce cDNA. ..

Article Title: Effect of chronic THC administration in the reproductive organs of male mice, spermatozoa and in vitro fertilization.
Article Snippet: Accepted Manuscript Effect of chronic THC administration in the reproductive organs of male mice, spermatozoa and in vitro fertilization A.P.. López-Cardona, I. Ibarra-Lecue, R. Laguna-Barraza, S. Pérez-Cerezales, L. Urigüen, N. Agirregoitia, A. Gutierrez-Adán, E. Agirregoitia PII: S0006-2952(18)30315-0 DOI: https://doi.org/10.1016/j.bcp.2018.07.045 Reference: BCP 13228 To appear in: Biochemical Pharmacology Received Date: 4 May 2018 Accepted Date: 31 July 2018 Please cite this article as: A.P.. López-Cardona, I. Ibarra-Lecue, R. Laguna-Barraza, S. Pérez-Cerezales, L. Urigüen, N. Agirregoitia, A. Gutierrez-Adán, E. Agirregoitia, Effect of chronic THC administration in the reproductive organs of male mice, spermatozoa and in vitro fertilization, Biochemical Pharmacology (2018), doi: https://doi.org/10.1016/j.bcp.2018.07.045 This is a PDF file of an unedited manuscript that has been accepted for publication.

Article Title: Expression and localization of ARTEMIN in the bovine uterus and embryos.
Article Snippet: Accepted Manuscript Expression and localization of ARTEMIN in the bovine uterus and embryos E. Gómez, D. Martin, S. Carrocera, M.J. Sánchez-Calabuig, A. Gutierrez-Adán, M. Alonso-Guervos, N. Peynot, C. Giraud-Delville, O. Sandra, V. Duranthon, M. Muñoz PII: S0093-691X(16)30580-5 DOI: 10.1016/j.theriogenology.2016.12.007 Reference: THE 13925 To appear in: Theriogenology Received Date: 9 September 2016 Revised Date: 29 November 2016 Accepted Date: 3 December 2016 Please cite this article as: Gómez E, Martin D, Carrocera S, Sánchez-Calabuig MJ, Gutierrez-Adán A, Alonso-Guervos M, Peynot N, Giraud-Delville C, Sandra O, Duranthon V, Muñoz M, Expression and localization of ARTEMIN in the bovine uterus and embryos, Theriogenology (2017), doi: 10.1016/ j.theriogenology.2016.12.007.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

Article Title: Resveratrol-cyclodextrin complex affects the expression of genes associated with lipid metabolism in bovine in vitro produced embryos.
Article Snippet: In vitro fertilization (IVF), manipulation of gametes and embryos and in vitro culture conditions expose cells to environmental factors that may affect antioxidant defence mechanism and therefore, negatively impacting the oocyte and the subsequent embryo, compared with those produced in vivo (Agarwal, Durairajanayagam, & du Plessis, 2014).. To improve the quality of the oocyte and the developmental capacities of embryos cultured in vitro, antioxidant compounds have been used to supplement the culture medium.. One of the most important polyphenols found in red wine is resveratrol (3,4,5trihydroxytransstilbene).

other:

Article Title: In vitro cultured bovine endometrial cells recognize embryonic sex.
Article Snippet: 19 Endometrial cell co-culture (ECC) with single embryo may reflect endometrium responses in 20 vivo.. Bovine Day-6 in vitro-produced morulae were cultured until Day-8 in modified synthetic 21 oviductal fluid (mSOF), or on the epithelial side of ECC.. Expression of epithelialand stromal22 cell transcripts was analyzed by RT-PCR in ECC with one male (ME) or female embryo (FE).

Article Title: MicroRNA-148b secreted by bovine oviductal extracellular vesicles enhance embryo quality through BPM/TGF-beta pathway.
Article Snippet: Tubes were heated to 70 oC for 5 min to denature the secondary RNA structure, allowing Poly(T) random primers and Oligo dT annealing, and the RT mix was then completed by adding 0.375 mM dNTPs (Biotools, Madrid, Spain), 6.25 U RNAsin RNAse inhibitor (Promega, Madison, WI, USA), MMLV HP RT 10x reaction buffer, 5 mM DTT and 5 U MMLV highperformance reverse transcriptase (Epicentre Technologies Corp, Madison, WI, USA).

Purification:

Article Title: Expression and localization of ARTEMIN in the bovine uterus and embryos.
Article Snippet: Accepted Manuscript Expression and localization of ARTEMIN in the bovine uterus and embryos E. Gómez, D. Martin, S. Carrocera, M.J. Sánchez-Calabuig, A. Gutierrez-Adán, M. Alonso-Guervos, N. Peynot, C. Giraud-Delville, O. Sandra, V. Duranthon, M. Muñoz PII: S0093-691X(16)30580-5 DOI: 10.1016/j.theriogenology.2016.12.007 Reference: THE 13925 To appear in: Theriogenology Received Date: 9 September 2016 Revised Date: 29 November 2016 Accepted Date: 3 December 2016 Please cite this article as: Gómez E, Martin D, Carrocera S, Sánchez-Calabuig MJ, Gutierrez-Adán A, Alonso-Guervos M, Peynot N, Giraud-Delville C, Sandra O, Duranthon V, Muñoz M, Expression and localization of ARTEMIN in the bovine uterus and embryos, Theriogenology (2017), doi: 10.1016/ j.theriogenology.2016.12.007.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.



Similar Products

95
New England Biolabs e coli rna polymerase core enzyme
E Coli Rna Polymerase Core Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transcriptase+enzyme/E%2Ecoli+RNA+Polymerase%2C+Core+Enzyme/pm42816508-733-9-19
Average 95 stars, based on 1 article reviews
e coli rna polymerase core enzyme - by Bioz Stars, 2026-11
95/100 stars
  Buy from Supplier

99
New England Biolabs m mulv reverse transcriptase enzyme
M Mulv Reverse Transcriptase Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transcriptase+enzyme/M-MuLV+Reverse+Transcriptase/pm42543510-86-13-17
Average 99 stars, based on 1 article reviews
m mulv reverse transcriptase enzyme - by Bioz Stars, 2026-11
99/100 stars
  Buy from Supplier

99
RNAConnect Inc ultramarathonrt enzyme
Ultramarathonrt Enzyme, supplied by RNAConnect Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transcriptase+enzyme/uMRT+Reverse+Transcriptase/bio_rxiv__64898__2026__05__21__726978-230-1-3
Average 99 stars, based on 1 article reviews
ultramarathonrt enzyme - by Bioz Stars, 2026-11
99/100 stars
  Buy from Supplier

95
New England Biolabs rna polymerase core enzyme
Rna Polymerase Core Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transcriptase+enzyme/E%2Ecoli+RNA+Pol%2C+Core+Enzyme/pm42003578-163-0-7
Average 95 stars, based on 1 article reviews
rna polymerase core enzyme - by Bioz Stars, 2026-11
95/100 stars
  Buy from Supplier

95
New England Biolabs e coli rnap core enzyme
Several RNAs transcribed by the sibD minimal promoter from <t>E.</t> <t>coli</t> chromosomal DNA could be NAD capped. ( A ) Schematic illustration of gene editing designs in the E. coli genome for four small RNAs expression driven by the sibD minimal promoter. The gene body of trpT is highlighted in purple, sroC in green, and ryjA and symR in blue. The sibD minimal promoter ( sibD P-35 ) is labelled as a short red line, and the rrnB terminator is highlighted in yellow. ( B ) Detection of NAD caps in SibD, TrpT, RyjA, SroC, and SymR RNAs with NADbio-northern blotting in the wild-type and indicated mutant strains. ‘ADPRC+’ indicates the biotinylation of NAD-RNAs via the ADPRC-SPAAC reaction with sufficient ADPRC, while ‘ADPRC−’ denotes the ADPRC-SPAAC reaction without ADPRC. 5S RNAs were detected as loading controls. ( C ) Detection and quantification of NAD-RNAs from SibD, TrpT, RyjA, SroC, and SymR using APB gel blotting. Capping ratios were calculated based on the band intensity of the capped transcripts relative to the total transcripts (both capped and uncapped transcripts) in the APB gel.
E Coli Rnap Core Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transcriptase+enzyme/E%2Ecoli+RNA+Polymerase%2C+Core+Enzyme/pmc12956327-111-26-31
Average 95 stars, based on 1 article reviews
e coli rnap core enzyme - by Bioz Stars, 2026-11
95/100 stars
  Buy from Supplier

99
New England Biolabs m mulv reverse transcriptase rt enzyme
Several RNAs transcribed by the sibD minimal promoter from <t>E.</t> <t>coli</t> chromosomal DNA could be NAD capped. ( A ) Schematic illustration of gene editing designs in the E. coli genome for four small RNAs expression driven by the sibD minimal promoter. The gene body of trpT is highlighted in purple, sroC in green, and ryjA and symR in blue. The sibD minimal promoter ( sibD P-35 ) is labelled as a short red line, and the rrnB terminator is highlighted in yellow. ( B ) Detection of NAD caps in SibD, TrpT, RyjA, SroC, and SymR RNAs with NADbio-northern blotting in the wild-type and indicated mutant strains. ‘ADPRC+’ indicates the biotinylation of NAD-RNAs via the ADPRC-SPAAC reaction with sufficient ADPRC, while ‘ADPRC−’ denotes the ADPRC-SPAAC reaction without ADPRC. 5S RNAs were detected as loading controls. ( C ) Detection and quantification of NAD-RNAs from SibD, TrpT, RyjA, SroC, and SymR using APB gel blotting. Capping ratios were calculated based on the band intensity of the capped transcripts relative to the total transcripts (both capped and uncapped transcripts) in the APB gel.
M Mulv Reverse Transcriptase Rt Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transcriptase+enzyme/M-MuLV+Reverse+Transcriptase/pm41630844-123-26-34
Average 99 stars, based on 1 article reviews
m mulv reverse transcriptase rt enzyme - by Bioz Stars, 2026-11
99/100 stars
  Buy from Supplier

97
Qiagen superscript reverse transcriptase enzyme
Several RNAs transcribed by the sibD minimal promoter from <t>E.</t> <t>coli</t> chromosomal DNA could be NAD capped. ( A ) Schematic illustration of gene editing designs in the E. coli genome for four small RNAs expression driven by the sibD minimal promoter. The gene body of trpT is highlighted in purple, sroC in green, and ryjA and symR in blue. The sibD minimal promoter ( sibD P-35 ) is labelled as a short red line, and the rrnB terminator is highlighted in yellow. ( B ) Detection of NAD caps in SibD, TrpT, RyjA, SroC, and SymR RNAs with NADbio-northern blotting in the wild-type and indicated mutant strains. ‘ADPRC+’ indicates the biotinylation of NAD-RNAs via the ADPRC-SPAAC reaction with sufficient ADPRC, while ‘ADPRC−’ denotes the ADPRC-SPAAC reaction without ADPRC. 5S RNAs were detected as loading controls. ( C ) Detection and quantification of NAD-RNAs from SibD, TrpT, RyjA, SroC, and SymR using APB gel blotting. Capping ratios were calculated based on the band intensity of the capped transcripts relative to the total transcripts (both capped and uncapped transcripts) in the APB gel.
Superscript Reverse Transcriptase Enzyme, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transcriptase+enzyme/Reverse+transcriptase/10__1016_slash_j__crpvbd__2026__100358-82-48-53
Average 97 stars, based on 1 article reviews
superscript reverse transcriptase enzyme - by Bioz Stars, 2026-11
97/100 stars
  Buy from Supplier

Image Search Results


Several RNAs transcribed by the sibD minimal promoter from E. coli chromosomal DNA could be NAD capped. ( A ) Schematic illustration of gene editing designs in the E. coli genome for four small RNAs expression driven by the sibD minimal promoter. The gene body of trpT is highlighted in purple, sroC in green, and ryjA and symR in blue. The sibD minimal promoter ( sibD P-35 ) is labelled as a short red line, and the rrnB terminator is highlighted in yellow. ( B ) Detection of NAD caps in SibD, TrpT, RyjA, SroC, and SymR RNAs with NADbio-northern blotting in the wild-type and indicated mutant strains. ‘ADPRC+’ indicates the biotinylation of NAD-RNAs via the ADPRC-SPAAC reaction with sufficient ADPRC, while ‘ADPRC−’ denotes the ADPRC-SPAAC reaction without ADPRC. 5S RNAs were detected as loading controls. ( C ) Detection and quantification of NAD-RNAs from SibD, TrpT, RyjA, SroC, and SymR using APB gel blotting. Capping ratios were calculated based on the band intensity of the capped transcripts relative to the total transcripts (both capped and uncapped transcripts) in the APB gel.

Journal: Nucleic Acids Research

Article Title: NAD + capping of sibD transcripts in E. coli is mediated by its minimal promoter and enhanced by ppGpp

doi: 10.1093/nar/gkag102

Figure Lengend Snippet: Several RNAs transcribed by the sibD minimal promoter from E. coli chromosomal DNA could be NAD capped. ( A ) Schematic illustration of gene editing designs in the E. coli genome for four small RNAs expression driven by the sibD minimal promoter. The gene body of trpT is highlighted in purple, sroC in green, and ryjA and symR in blue. The sibD minimal promoter ( sibD P-35 ) is labelled as a short red line, and the rrnB terminator is highlighted in yellow. ( B ) Detection of NAD caps in SibD, TrpT, RyjA, SroC, and SymR RNAs with NADbio-northern blotting in the wild-type and indicated mutant strains. ‘ADPRC+’ indicates the biotinylation of NAD-RNAs via the ADPRC-SPAAC reaction with sufficient ADPRC, while ‘ADPRC−’ denotes the ADPRC-SPAAC reaction without ADPRC. 5S RNAs were detected as loading controls. ( C ) Detection and quantification of NAD-RNAs from SibD, TrpT, RyjA, SroC, and SymR using APB gel blotting. Capping ratios were calculated based on the band intensity of the capped transcripts relative to the total transcripts (both capped and uncapped transcripts) in the APB gel.

Article Snippet: To perform IVT assays with various sigma factors, a similar assay was conducted, except the E. coli RNAP holoenzyme was replaced by the same amount of E. coli RNAP core enzyme (NEB) and in the absence of ppGpp or DksA.

Techniques: Expressing, Northern Blot, Mutagenesis

Effects of (p)ppGpp and DksA on transcription and NAD capping of certain small RNAs in E. coli cells. ( A ) The NAD capping level of SibD increased upon transient induction of RelA 455aa and DksA. Both RelA 455aa and DksA were expressed from plasmids under the control of the pBAD promoter. NAD capping of SibD was assessed by APB gel blotting. The total level of SibD RNA in each lane was quantified from the normal gel using ImageJ software and normalized to the intensity in the first EV lane. The NAD capping ratio was calculated as the percentage of the intensity of the NAD-capped band relative to the sum of the intensities of both the capped and uncapped bands in the APB gel. ‘Arabinose−’ indicates RNA samples without arabinose induction, while ‘Arabinose+’ signifies that arabinose was added to induce the expression of RelA 455aa and DksA. ‘EV’ indicates strain carrying the empty pBAD33.1 vector. The tmRNA was used as a loading control and each blotting has three independent replicates. ( B ) Detection of NAD-capped transcripts of five sRNAs with NADbio-northern blotting analysis, including four known NAD-RNAs: SibC, SibD, SibE, and GcvB. The tmRNA was used as a loading control. ( C – G ) Detection of total transcripts and NAD-capped transcripts of five sRNAs, namely SibA, SibC, SibD, SibE, and GcvB, respectively. The total abundance of individual RNA was determined by electrophoresis on a standard PAGE gel followed by northern blotting (labelled as normal gel), while the NAD-capped transcripts were identified with APB gel blotting (labelled as APB gel). The non-NAD-RNA SibA was included as a negative control. The NAD capping ratio was calculated based on the band intensity of the NAD-capped version relative to the total transcription levels (NAD-capped version plus uncapped version). Two types of synthetic RNAs for each sRNA, namely with 5′-ppp- and 5′-NAD modifications, were used as controls.

Journal: Nucleic Acids Research

Article Title: NAD + capping of sibD transcripts in E. coli is mediated by its minimal promoter and enhanced by ppGpp

doi: 10.1093/nar/gkag102

Figure Lengend Snippet: Effects of (p)ppGpp and DksA on transcription and NAD capping of certain small RNAs in E. coli cells. ( A ) The NAD capping level of SibD increased upon transient induction of RelA 455aa and DksA. Both RelA 455aa and DksA were expressed from plasmids under the control of the pBAD promoter. NAD capping of SibD was assessed by APB gel blotting. The total level of SibD RNA in each lane was quantified from the normal gel using ImageJ software and normalized to the intensity in the first EV lane. The NAD capping ratio was calculated as the percentage of the intensity of the NAD-capped band relative to the sum of the intensities of both the capped and uncapped bands in the APB gel. ‘Arabinose−’ indicates RNA samples without arabinose induction, while ‘Arabinose+’ signifies that arabinose was added to induce the expression of RelA 455aa and DksA. ‘EV’ indicates strain carrying the empty pBAD33.1 vector. The tmRNA was used as a loading control and each blotting has three independent replicates. ( B ) Detection of NAD-capped transcripts of five sRNAs with NADbio-northern blotting analysis, including four known NAD-RNAs: SibC, SibD, SibE, and GcvB. The tmRNA was used as a loading control. ( C – G ) Detection of total transcripts and NAD-capped transcripts of five sRNAs, namely SibA, SibC, SibD, SibE, and GcvB, respectively. The total abundance of individual RNA was determined by electrophoresis on a standard PAGE gel followed by northern blotting (labelled as normal gel), while the NAD-capped transcripts were identified with APB gel blotting (labelled as APB gel). The non-NAD-RNA SibA was included as a negative control. The NAD capping ratio was calculated based on the band intensity of the NAD-capped version relative to the total transcription levels (NAD-capped version plus uncapped version). Two types of synthetic RNAs for each sRNA, namely with 5′-ppp- and 5′-NAD modifications, were used as controls.

Article Snippet: To perform IVT assays with various sigma factors, a similar assay was conducted, except the E. coli RNAP holoenzyme was replaced by the same amount of E. coli RNAP core enzyme (NEB) and in the absence of ppGpp or DksA.

Techniques: Control, Software, Expressing, Plasmid Preparation, Northern Blot, Electrophoresis, Negative Control