mmlv high-performance reverse transcriptase (Epicentre Technologies Corp)
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Mmlv High Performance Reverse Transcriptase, supplied by Epicentre Technologies Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transcriptase+enzyme/mmlv+high+performance+reverse+transcriptase+enzyme/pm38520036-112-59-63
Average 90 stars, based on 1 article reviews
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Extraction:Article Title: Oxygen tension during in vitro oocyte maturation and fertilization affects embryo quality in sheep and deer. Article Snippet: Incubation gas atmosphere affects the development of in vitro produced embryos.. In this study, there was examination of effects of two different oxygen (O2) tensions (5 % and 21 %) during in vitro maturation (M5 and M21) and/or fertilization (F5 and F21) on embryo production and quality in deer and sheep.. There was assessment of the percentage of embryos with cell cleavage occurring, percentage that developed to the blastocyst stage, and analysis of the relative abundance of mRNA transcript for genes important for development to the blastocyst stage. Article Title: Hepatoma-derived growth factor: Protein quantification in uterine fluid, gene expression in endometrial-cell culture and effects on in vitro embryo development, pregnancy and birth. Article Snippet: Hepatoma-derived growth factor (HDGF) is present in the endometrium of cows and other mammals.. Recombinant HDGF (rHDGF) improves bovine blastocyst development in vitro.. However, specific culture conditions and essential aspects of HDGF uterine physiology are yet unknown. Article Title: Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2 Article Snippet: .. After extraction, RT reaction was carried out following the manufacturer’s instructions (Epicentre Technologies Corp, Madison, WI, USA) using poly(T) primers, random primers and Article Title: Resveratrol-cyclodextrin complex affects the expression of genes associated with lipid metabolism in bovine in vitro produced embryos. Article Snippet: In vitro fertilization (IVF), manipulation of gametes and embryos and in vitro culture conditions expose cells to environmental factors that may affect antioxidant defence mechanism and therefore, negatively impacting the oocyte and the subsequent embryo, compared with those produced in vivo (Agarwal, Durairajanayagam, & du Plessis, 2014).. To improve the quality of the oocyte and the developmental capacities of embryos cultured in vitro, antioxidant compounds have been used to supplement the culture medium.. One of the most important polyphenols found in red wine is resveratrol (3,4,5trihydroxytransstilbene). Reverse Transcription:Article Title: Oxygen tension during in vitro oocyte maturation and fertilization affects embryo quality in sheep and deer. Article Snippet: Incubation gas atmosphere affects the development of in vitro produced embryos.. In this study, there was examination of effects of two different oxygen (O2) tensions (5 % and 21 %) during in vitro maturation (M5 and M21) and/or fertilization (F5 and F21) on embryo production and quality in deer and sheep.. There was assessment of the percentage of embryos with cell cleavage occurring, percentage that developed to the blastocyst stage, and analysis of the relative abundance of mRNA transcript for genes important for development to the blastocyst stage. Article Title: Hepatoma-derived growth factor: Protein quantification in uterine fluid, gene expression in endometrial-cell culture and effects on in vitro embryo development, pregnancy and birth. Article Snippet: Hepatoma-derived growth factor (HDGF) is present in the endometrium of cows and other mammals.. Recombinant HDGF (rHDGF) improves bovine blastocyst development in vitro.. However, specific culture conditions and essential aspects of HDGF uterine physiology are yet unknown. Article Title: Exocannabinoids effect on in vitro bovine oocyte maturation via activation of AKT and ERK1/2 Article Snippet: .. After extraction, RT reaction was carried out following the manufacturer’s instructions (Epicentre Technologies Corp, Madison, WI, USA) using poly(T) primers, random primers and Article Title: Effect of chronic THC administration in the reproductive organs of male mice, spermatozoa and in vitro fertilization. Article Snippet: Accepted Manuscript Effect of chronic THC administration in the reproductive organs of male mice, spermatozoa and in vitro fertilization A.P.. López-Cardona, I. Ibarra-Lecue, R. Laguna-Barraza, S. Pérez-Cerezales, L. Urigüen, N. Agirregoitia, A. Gutierrez-Adán, E. Agirregoitia PII: S0006-2952(18)30315-0 DOI: https://doi.org/10.1016/j.bcp.2018.07.045 Reference: BCP 13228 To appear in: Biochemical Pharmacology Received Date: 4 May 2018 Accepted Date: 31 July 2018 Please cite this article as: A.P.. López-Cardona, I. Ibarra-Lecue, R. Laguna-Barraza, S. Pérez-Cerezales, L. Urigüen, N. Agirregoitia, A. Gutierrez-Adán, E. Agirregoitia, Effect of chronic THC administration in the reproductive organs of male mice, spermatozoa and in vitro fertilization, Biochemical Pharmacology (2018), doi: https://doi.org/10.1016/j.bcp.2018.07.045 This is a PDF file of an unedited manuscript that has been accepted for publication. Article Title: Expression and localization of ARTEMIN in the bovine uterus and embryos. Article Snippet: Accepted Manuscript Expression and localization of ARTEMIN in the bovine uterus and embryos E. Gómez, D. Martin, S. Carrocera, M.J. Sánchez-Calabuig, A. Gutierrez-Adán, M. Alonso-Guervos, N. Peynot, C. Giraud-Delville, O. Sandra, V. Duranthon, M. Muñoz PII: S0093-691X(16)30580-5 DOI: 10.1016/j.theriogenology.2016.12.007 Reference: THE 13925 To appear in: Theriogenology Received Date: 9 September 2016 Revised Date: 29 November 2016 Accepted Date: 3 December 2016 Please cite this article as: Gómez E, Martin D, Carrocera S, Sánchez-Calabuig MJ, Gutierrez-Adán A, Alonso-Guervos M, Peynot N, Giraud-Delville C, Sandra O, Duranthon V, Muñoz M, Expression and localization of ARTEMIN in the bovine uterus and embryos, Theriogenology (2017), doi: 10.1016/ j.theriogenology.2016.12.007.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript. Article Title: Resveratrol-cyclodextrin complex affects the expression of genes associated with lipid metabolism in bovine in vitro produced embryos. Article Snippet: In vitro fertilization (IVF), manipulation of gametes and embryos and in vitro culture conditions expose cells to environmental factors that may affect antioxidant defence mechanism and therefore, negatively impacting the oocyte and the subsequent embryo, compared with those produced in vivo (Agarwal, Durairajanayagam, & du Plessis, 2014).. To improve the quality of the oocyte and the developmental capacities of embryos cultured in vitro, antioxidant compounds have been used to supplement the culture medium.. One of the most important polyphenols found in red wine is resveratrol (3,4,5trihydroxytransstilbene). other:Article Title: In vitro cultured bovine endometrial cells recognize embryonic sex. Article Snippet: 19 Endometrial cell co-culture (ECC) with single embryo may reflect endometrium responses in 20 vivo.. Bovine Day-6 in vitro-produced morulae were cultured until Day-8 in modified synthetic 21 oviductal fluid (mSOF), or on the epithelial side of ECC.. Expression of epithelialand stromal22 cell transcripts was analyzed by RT-PCR in ECC with one male (ME) or female embryo (FE). Article Title: MicroRNA-148b secreted by bovine oviductal extracellular vesicles enhance embryo quality through BPM/TGF-beta pathway. Article Snippet: Tubes were heated to 70 oC for 5 min to denature the secondary RNA structure, allowing Poly(T) random primers and Oligo dT annealing, and the RT mix was then completed by adding 0.375 mM dNTPs (Biotools, Madrid, Spain), 6.25 U RNAsin RNAse inhibitor (Promega, Madison, WI, USA), MMLV HP RT 10x reaction buffer, 5 mM DTT and 5 U Purification:Article Title: Expression and localization of ARTEMIN in the bovine uterus and embryos. Article Snippet: Accepted Manuscript Expression and localization of ARTEMIN in the bovine uterus and embryos E. Gómez, D. Martin, S. Carrocera, M.J. Sánchez-Calabuig, A. Gutierrez-Adán, M. Alonso-Guervos, N. Peynot, C. Giraud-Delville, O. Sandra, V. Duranthon, M. Muñoz PII: S0093-691X(16)30580-5 DOI: 10.1016/j.theriogenology.2016.12.007 Reference: THE 13925 To appear in: Theriogenology Received Date: 9 September 2016 Revised Date: 29 November 2016 Accepted Date: 3 December 2016 Please cite this article as: Gómez E, Martin D, Carrocera S, Sánchez-Calabuig MJ, Gutierrez-Adán A, Alonso-Guervos M, Peynot N, Giraud-Delville C, Sandra O, Duranthon V, Muñoz M, Expression and localization of ARTEMIN in the bovine uterus and embryos, Theriogenology (2017), doi: 10.1016/ j.theriogenology.2016.12.007.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript. |
