reverse transcription kit (product number: ae311-02)  (TransGen biotech co)

Journal: Gut Microbes

Article Title: Disentangling the impact of obesity, diet, host factors, and microbiota on small intestinal antimicrobial peptide expression

doi: 10.1080/19490976.2025.2536095

Figure Lengend Snippet: Correlations between AMP expression and mucosal small intestinal microbiota revealed a novel microbe capable of inducing Reg3g expression.

Article Snippet: Different factors can impact AMP expression in the murine small intestine. (a, b, c) Schematic experimental setup of the dietary intervention experiments: (a) Intervention 1, male Charles River mice fed a chow or a high-fat diet (HFD) for 8 weeks; (b) Intervention 2, male and female Charles River mice fed a chow or a Western-style diet (WSD) for 8 weeks; (c) Intervention 3, male and female Taconic mice fed a chow or a WSD for 8 weeks. (d) Total amounts of all measured AMP transcripts in the ileum of all mice fed a chow, WSD, or HFD. (e) Total amounts of all measured AMP transcripts in the ileum of male and female mice fed a chow or WSD from interventions 2 and 3. (f) Reg3g transcript copy number in the ileum of chow and WSD fed mice from interventions 2 and 3. (g) Total amounts of all measured AMP transcripts in the ileum of male Charles River or Taconic mice fed a chow or a WSD. (h) Reg3g transcript copy number in the ileum of male Charles River or Taconic mice fed a chow or a WSD. (i) Total amounts of all measured AMP transcripts in the ileum of female Charles River or Taconic mice fed a chow or a WSD. (j) Reg3g transcript copy number in the ileum of female Charles River or Taconic mice fed a chow or a WSD. (k) Principal component analysis (PCoA) evaluating the effect of diet, mouse vendor, sex, or intervention on AMP expression in mice from the dietary interventions.

Techniques: Expressing

Journal: Scientific Reports

Article Title: ALKBH5, an m6A demethylase, attenuates tumor growth and inhibits metastasis in papillary thyroid carcinoma

doi: 10.1038/s41598-024-84352-w

Figure Lengend Snippet: Patient demographics and tumor characteristics and association of ALKBH5 levels with clinicopathological features.

Article Snippet: The CDS sequence of the ALKBH5 gene (Transcript accession number: NM_017758.4) was retrieved from the National Center for Biotechnology Information.

Techniques: Expressing

Journal: Scientific Reports

Article Title: ALKBH5, an m6A demethylase, attenuates tumor growth and inhibits metastasis in papillary thyroid carcinoma

doi: 10.1038/s41598-024-84352-w

Figure Lengend Snippet: ( A ) qRT-PCR and Western blot were used to detect the expression of ALKBH5 to screen PTC cells. ( * P < 0.05 indicates a significant difference between each group compared to the Nthy-ori3-1 group). ( B ) Subcellular Localization of ALKBH5 Protein Detected by Immunofluorescence.

Article Snippet: The CDS sequence of the ALKBH5 gene (Transcript accession number: NM_017758.4) was retrieved from the National Center for Biotechnology Information.

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Immunofluorescence

Journal: Scientific Reports

Article Title: ALKBH5, an m6A demethylase, attenuates tumor growth and inhibits metastasis in papillary thyroid carcinoma

doi: 10.1038/s41598-024-84352-w

Figure Lengend Snippet: Transfection validation of ALKBH5 overexpression and interference ( A . IHH4 cells; B . TPC-1 cells).

Article Snippet: The CDS sequence of the ALKBH5 gene (Transcript accession number: NM_017758.4) was retrieved from the National Center for Biotechnology Information.

Techniques: Transfection, Biomarker Discovery, Over Expression

Journal: Scientific Reports

Article Title: ALKBH5, an m6A demethylase, attenuates tumor growth and inhibits metastasis in papillary thyroid carcinoma

doi: 10.1038/s41598-024-84352-w

Figure Lengend Snippet: Proliferation and invasion of IHH4 and TPC-1 Cells Detected by CCK-8 Assay and Transwell Assay ( A . CCK-8 assay to detect the cell viability of IHH4 and TPC-1 cells; B . Transwell assay to assess the invasion of IHH4 cells; C . Transwell assay to assess the invasion of TPC-1 cells. * P < 0.05 indicates a significant difference between each group compared to the OE-NC group; # P < 0.05 indicates a significant difference between each group compared to the OE-ALKBH5 group; & P < 0.05 indicates a significant difference between each group compared to the Si-NC group).

Article Snippet: The CDS sequence of the ALKBH5 gene (Transcript accession number: NM_017758.4) was retrieved from the National Center for Biotechnology Information.

Techniques: CCK-8 Assay, Transwell Assay

Journal: Scientific Reports

Article Title: ALKBH5, an m6A demethylase, attenuates tumor growth and inhibits metastasis in papillary thyroid carcinoma

doi: 10.1038/s41598-024-84352-w

Figure Lengend Snippet: m6A Methylation detection (A for IHH4 Cells; B for TPC-1 Cells. * P < 0.05 indicates a significant difference between each group compared to the OE-NC group; # P < 0.05 indicates a significant difference between each group compared to the OE-ALKBH5 group; & P < 0.05 indicates a significant difference between each group compared to the Si-NC group).

Article Snippet: The CDS sequence of the ALKBH5 gene (Transcript accession number: NM_017758.4) was retrieved from the National Center for Biotechnology Information.

Techniques: Methylation