gene exp galnt12 hs00226436 m1 (Thermo Fisher)
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gene exp galnt12 hs00226436 m1
Gene Exp Galnt12 Hs00226436 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/total+human+1%2E2+cdna+microarray/Gene+Exp%2E+GALNT12%2C+Hs00226436_m1/pmc06523947-110-45-9
Average 95 stars, based on 1 article reviews
Gene Exp Galnt12 Hs00226436 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/total+human+1%2E2+cdna+microarray/Gene+Exp%2E+GALNT12%2C+Hs00226436_m1/pmc06523947-110-45-9
Average 95 stars, based on 1 article reviews
gene exp galnt12 hs00226436 m1 - by Bioz Stars,
2026-10
95/100 stars
Images
1) Product Images from "Transcriptomic Response to 1,25-Dihydroxyvitamin D in Human Fibroblasts with or without a Functional Vitamin D Receptor (VDR): Novel Target Genes and Insights into VDR Basal Transcriptional Activity"
Article Title: Transcriptomic Response to 1,25-Dihydroxyvitamin D in Human Fibroblasts with or without a Functional Vitamin D Receptor (VDR): Novel Target Genes and Insights into VDR Basal Transcriptional Activity
Journal: Cells
doi: 10.3390/cells8040318
Figure Legend Snippet: Top-ranking up- and down- regulated 1,25D-activated VDR target genes in human fibroblasts (CO).
Techniques Used:
Figure Legend Snippet: Quantitative RT-PCR validation of differential gene expression findings.
Techniques Used: Quantitative RT-PCR, Biomarker Discovery, Gene Expression, Quantitative Proteomics, Microarray
Related Articles
Quantitative RT-PCR:Article Title: The Role of Dimethyl Sulfoxide (DMSO) in Gene Expression Modulation and Glycosaminoglycan Metabolism in Lysosomal Storage Disorders on an Example of Mucopolysaccharidosis Article Snippet: Total RNA was reverse-transcribed into cDNA using Transcriptor First Strand cDNA Synthesis Kit (Roche Applied Science, Indianapolis, IN, USA), according to the manufacturer’s instructions. .. Real-time qRT-PCR was carried out with TaqMan Gene Expression Assays (Thermo Fisher Scientific, Foster City, CA, USA) with catalog numbers as follows: #4426961 (Hs00904548_m1, ALG2 ), #4331182 (Hs00970128_m1, ALG12 ), #4331182 (Hs00406036_m1, ARSK ), #4331182 (Hs00155245_m1, B4GALT1 ), #4331182 (Hs01921028_s1, CHST2 ), #4331182 (Hs00248144_m1, CHST15 ), #4331182 (Hs00609162_m1, EXT1 ), #4331182 (Hs00975732_m1, GALNS ), #4331182 (Hs00189537_m1, GALNT2 ), #4331182 (Hs00559726_s1, GALNT4 ), #4331182 (Hs99999909_m1, GALNT6 ), #4331182 ( Gene Expression:Article Title: The Role of Dimethyl Sulfoxide (DMSO) in Gene Expression Modulation and Glycosaminoglycan Metabolism in Lysosomal Storage Disorders on an Example of Mucopolysaccharidosis Article Snippet: Total RNA was reverse-transcribed into cDNA using Transcriptor First Strand cDNA Synthesis Kit (Roche Applied Science, Indianapolis, IN, USA), according to the manufacturer’s instructions. .. Real-time qRT-PCR was carried out with TaqMan Gene Expression Assays (Thermo Fisher Scientific, Foster City, CA, USA) with catalog numbers as follows: #4426961 (Hs00904548_m1, ALG2 ), #4331182 (Hs00970128_m1, ALG12 ), #4331182 (Hs00406036_m1, ARSK ), #4331182 (Hs00155245_m1, B4GALT1 ), #4331182 (Hs01921028_s1, CHST2 ), #4331182 (Hs00248144_m1, CHST15 ), #4331182 (Hs00609162_m1, EXT1 ), #4331182 (Hs00975732_m1, GALNS ), #4331182 (Hs00189537_m1, GALNT2 ), #4331182 (Hs00559726_s1, GALNT4 ), #4331182 (Hs99999909_m1, GALNT6 ), #4331182 ( Article Title: Excess APP O-glycosylation by GalNAc-T6 decreases Aβ production. Article Snippet: SuperScript II RNase H- Reverse Transcriptase and random primers (Thermo Fisher Scientific) were used to synthesize first-strand cDNAs from 5 mg of total RNA. .. TaqMan real-time RTPCR assay on a 7300 Fast Real-Time PCR System (Thermo Fisher Scientific) were performed for relative quantification of the target mRNA following the manufacturer’s instructions using the TaqMan Universal PCR Master Mix and TaqMan Gene Expression Assays: GalNAc-T1, Hs00234919_m1; GalNAc-T2, Hs00189537_m1; GalNAc-T3, Hs00237084_m1; GalNAc-T4, Hs00236809_s1; GalNAc-T5, Hs00294826_m1; GalNAc-T6, Hs00200529_m1, GalNAc-T7, Hs00213624_m1; GalNAc-T8, Hs00213610_m1; GalNAc-T9, Hs00222357_m1; GalNAc-T10, Hs00213903_m1; GalNAc-T11, Hs00254410_m1; GalNAc-T12, Polymerase Chain Reaction:Article Title: The GalNAc-T Activation Pathway (GALA) is not a general mechanism for regulating mucin-type O-glycosylation Article Snippet: RT-qPCR was performed using Taqman ® Gene expression assay (ThermoFisher Scientific, Waltham, MA) following manufacturer procedure. .. The PCR was performed using TaqMan ® Universal PCR Master Mix (ThermoFisher Scientific, Waltham, MA), with 50 ng of cDNA and the following probes: Actin B (Hs99999903_m1) was used as a positive control, and used to normalization for the relative expression of GalNAc-T1 (Hs00234919_m1), GalNAc-T2 (Hs00189537_m1), GalNAc-T3 (Hs00237084_m1), GalNAc-T4 (Hs00559726_s1), GalNAc-T5 (Hs00294826_m1), GalNAc-T6 (Hs00200529_m1), GalNAc-T7 (Hs00213624_m1), GalNAc-T8 (Hs00213610_m1), GalNAc-T9 (Hs00222357_m1), GalNAc-T10 (Hs00213903_m1), GalNAc-T11 (Hs00757865_m1), GalNAc-T12 ( Article Title: Growth Factors do not regulate Golgi Complex-to-ER relocation of GalNAc-Ts in HeLa cells Article Snippet: RT-qPCR was performed using Taqman ® Gene expression assay (ThermoFisher Scientific, Waltham, MA) following manufacturer procedure. .. The PCR was performed using TaqMan ® Universal PCR Master Mix (ThermoFisher Scientific, Waltham, MA), with 50 ng of cDNA and the following probes: Actin B (Hs99999903_m1) was used as a positive control, and used to normalization for the relative expression of GalNAc-T1 (Hs00234919_m 1), GalNAc-T2 (Hs00189537_m1), GalNAc-T3 (Hs00237084_m1), GalNAc-T4 (Hs00559726_s1), GalNAc-T5 (Hs00294826_m1), GalNAc-T6 (Hs00200529_m1), GalNAc-T7 (Hs00213624_m1), GalNAc-T8 (Hs00213610_m1), GalNAc-T9 (Hs00222357_m1), GalNAc-T10 (Hs00213903_m 1), GalNAc-T11 (Hs00757865_m1), GalNAc-T12 ( Article Title: Excess APP O-glycosylation by GalNAc-T6 decreases Aβ production. Article Snippet: SuperScript II RNase H- Reverse Transcriptase and random primers (Thermo Fisher Scientific) were used to synthesize first-strand cDNAs from 5 mg of total RNA. .. TaqMan real-time RTPCR assay on a 7300 Fast Real-Time PCR System (Thermo Fisher Scientific) were performed for relative quantification of the target mRNA following the manufacturer’s instructions using the TaqMan Universal PCR Master Mix and TaqMan Gene Expression Assays: GalNAc-T1, Hs00234919_m1; GalNAc-T2, Hs00189537_m1; GalNAc-T3, Hs00237084_m1; GalNAc-T4, Hs00236809_s1; GalNAc-T5, Hs00294826_m1; GalNAc-T6, Hs00200529_m1, GalNAc-T7, Hs00213624_m1; GalNAc-T8, Hs00213610_m1; GalNAc-T9, Hs00222357_m1; GalNAc-T10, Hs00213903_m1; GalNAc-T11, Hs00254410_m1; GalNAc-T12, Positive Control:Article Title: The GalNAc-T Activation Pathway (GALA) is not a general mechanism for regulating mucin-type O-glycosylation Article Snippet: RT-qPCR was performed using Taqman ® Gene expression assay (ThermoFisher Scientific, Waltham, MA) following manufacturer procedure. .. The PCR was performed using TaqMan ® Universal PCR Master Mix (ThermoFisher Scientific, Waltham, MA), with 50 ng of cDNA and the following probes: Actin B (Hs99999903_m1) was used as a positive control, and used to normalization for the relative expression of GalNAc-T1 (Hs00234919_m1), GalNAc-T2 (Hs00189537_m1), GalNAc-T3 (Hs00237084_m1), GalNAc-T4 (Hs00559726_s1), GalNAc-T5 (Hs00294826_m1), GalNAc-T6 (Hs00200529_m1), GalNAc-T7 (Hs00213624_m1), GalNAc-T8 (Hs00213610_m1), GalNAc-T9 (Hs00222357_m1), GalNAc-T10 (Hs00213903_m1), GalNAc-T11 (Hs00757865_m1), GalNAc-T12 ( Article Title: Growth Factors do not regulate Golgi Complex-to-ER relocation of GalNAc-Ts in HeLa cells Article Snippet: RT-qPCR was performed using Taqman ® Gene expression assay (ThermoFisher Scientific, Waltham, MA) following manufacturer procedure. .. The PCR was performed using TaqMan ® Universal PCR Master Mix (ThermoFisher Scientific, Waltham, MA), with 50 ng of cDNA and the following probes: Actin B (Hs99999903_m1) was used as a positive control, and used to normalization for the relative expression of GalNAc-T1 (Hs00234919_m 1), GalNAc-T2 (Hs00189537_m1), GalNAc-T3 (Hs00237084_m1), GalNAc-T4 (Hs00559726_s1), GalNAc-T5 (Hs00294826_m1), GalNAc-T6 (Hs00200529_m1), GalNAc-T7 (Hs00213624_m1), GalNAc-T8 (Hs00213610_m1), GalNAc-T9 (Hs00222357_m1), GalNAc-T10 (Hs00213903_m 1), GalNAc-T11 (Hs00757865_m1), GalNAc-T12 ( Expressing:Article Title: The GalNAc-T Activation Pathway (GALA) is not a general mechanism for regulating mucin-type O-glycosylation Article Snippet: RT-qPCR was performed using Taqman ® Gene expression assay (ThermoFisher Scientific, Waltham, MA) following manufacturer procedure. .. The PCR was performed using TaqMan ® Universal PCR Master Mix (ThermoFisher Scientific, Waltham, MA), with 50 ng of cDNA and the following probes: Actin B (Hs99999903_m1) was used as a positive control, and used to normalization for the relative expression of GalNAc-T1 (Hs00234919_m1), GalNAc-T2 (Hs00189537_m1), GalNAc-T3 (Hs00237084_m1), GalNAc-T4 (Hs00559726_s1), GalNAc-T5 (Hs00294826_m1), GalNAc-T6 (Hs00200529_m1), GalNAc-T7 (Hs00213624_m1), GalNAc-T8 (Hs00213610_m1), GalNAc-T9 (Hs00222357_m1), GalNAc-T10 (Hs00213903_m1), GalNAc-T11 (Hs00757865_m1), GalNAc-T12 ( Article Title: Growth Factors do not regulate Golgi Complex-to-ER relocation of GalNAc-Ts in HeLa cells Article Snippet: RT-qPCR was performed using Taqman ® Gene expression assay (ThermoFisher Scientific, Waltham, MA) following manufacturer procedure. .. The PCR was performed using TaqMan ® Universal PCR Master Mix (ThermoFisher Scientific, Waltham, MA), with 50 ng of cDNA and the following probes: Actin B (Hs99999903_m1) was used as a positive control, and used to normalization for the relative expression of GalNAc-T1 (Hs00234919_m 1), GalNAc-T2 (Hs00189537_m1), GalNAc-T3 (Hs00237084_m1), GalNAc-T4 (Hs00559726_s1), GalNAc-T5 (Hs00294826_m1), GalNAc-T6 (Hs00200529_m1), GalNAc-T7 (Hs00213624_m1), GalNAc-T8 (Hs00213610_m1), GalNAc-T9 (Hs00222357_m1), GalNAc-T10 (Hs00213903_m 1), GalNAc-T11 (Hs00757865_m1), GalNAc-T12 ( Reverse Transcription Polymerase Chain Reaction:Article Title: Excess APP O-glycosylation by GalNAc-T6 decreases Aβ production. Article Snippet: SuperScript II RNase H- Reverse Transcriptase and random primers (Thermo Fisher Scientific) were used to synthesize first-strand cDNAs from 5 mg of total RNA. .. TaqMan real-time RTPCR assay on a 7300 Fast Real-Time PCR System (Thermo Fisher Scientific) were performed for relative quantification of the target mRNA following the manufacturer’s instructions using the TaqMan Universal PCR Master Mix and TaqMan Gene Expression Assays: GalNAc-T1, Hs00234919_m1; GalNAc-T2, Hs00189537_m1; GalNAc-T3, Hs00237084_m1; GalNAc-T4, Hs00236809_s1; GalNAc-T5, Hs00294826_m1; GalNAc-T6, Hs00200529_m1, GalNAc-T7, Hs00213624_m1; GalNAc-T8, Hs00213610_m1; GalNAc-T9, Hs00222357_m1; GalNAc-T10, Hs00213903_m1; GalNAc-T11, Hs00254410_m1; GalNAc-T12, Real-time Polymerase Chain Reaction:Article Title: Excess APP O-glycosylation by GalNAc-T6 decreases Aβ production. Article Snippet: SuperScript II RNase H- Reverse Transcriptase and random primers (Thermo Fisher Scientific) were used to synthesize first-strand cDNAs from 5 mg of total RNA. .. TaqMan real-time RTPCR assay on a 7300 Fast Real-Time PCR System (Thermo Fisher Scientific) were performed for relative quantification of the target mRNA following the manufacturer’s instructions using the TaqMan Universal PCR Master Mix and TaqMan Gene Expression Assays: GalNAc-T1, Hs00234919_m1; GalNAc-T2, Hs00189537_m1; GalNAc-T3, Hs00237084_m1; GalNAc-T4, Hs00236809_s1; GalNAc-T5, Hs00294826_m1; GalNAc-T6, Hs00200529_m1, GalNAc-T7, Hs00213624_m1; GalNAc-T8, Hs00213610_m1; GalNAc-T9, Hs00222357_m1; GalNAc-T10, Hs00213903_m1; GalNAc-T11, Hs00254410_m1; GalNAc-T12, Article Title: Transcriptomic Response to 1,25-Dihydroxyvitamin D in Human Fibroblasts with or without a Functional Vitamin D Receptor (VDR): Novel Target Genes and Insights into VDR Basal Transcriptional Activity Article Snippet: .. First-strand cDNA was generated using SuperScript III reverse transcriptase (Thermo Fisher) and quantitative PCR performed in a StepOnePlus Real-Time PCR System (Thermo Fisher) using the following inventoried TaqMan® assays (Thermo Fisher): CYP24A1 Hs00167999 _m1, TGFB2 Hs00234244_m1, RASSF2 Hs00248129_m1, CILP Hs00173647_m1, STC1 Hs00174970_m1, SPP1 Hs00959010_m1, GALNT12 Quantitative Proteomics:Article Title: Excess APP O-glycosylation by GalNAc-T6 decreases Aβ production. Article Snippet: SuperScript II RNase H- Reverse Transcriptase and random primers (Thermo Fisher Scientific) were used to synthesize first-strand cDNAs from 5 mg of total RNA. .. TaqMan real-time RTPCR assay on a 7300 Fast Real-Time PCR System (Thermo Fisher Scientific) were performed for relative quantification of the target mRNA following the manufacturer’s instructions using the TaqMan Universal PCR Master Mix and TaqMan Gene Expression Assays: GalNAc-T1, Hs00234919_m1; GalNAc-T2, Hs00189537_m1; GalNAc-T3, Hs00237084_m1; GalNAc-T4, Hs00236809_s1; GalNAc-T5, Hs00294826_m1; GalNAc-T6, Hs00200529_m1, GalNAc-T7, Hs00213624_m1; GalNAc-T8, Hs00213610_m1; GalNAc-T9, Hs00222357_m1; GalNAc-T10, Hs00213903_m1; GalNAc-T11, Hs00254410_m1; GalNAc-T12, Control:Article Title: Excess APP O-glycosylation by GalNAc-T6 decreases Aβ production. Article Snippet: SuperScript II RNase H- Reverse Transcriptase and random primers (Thermo Fisher Scientific) were used to synthesize first-strand cDNAs from 5 mg of total RNA. .. TaqMan real-time RTPCR assay on a 7300 Fast Real-Time PCR System (Thermo Fisher Scientific) were performed for relative quantification of the target mRNA following the manufacturer’s instructions using the TaqMan Universal PCR Master Mix and TaqMan Gene Expression Assays: GalNAc-T1, Hs00234919_m1; GalNAc-T2, Hs00189537_m1; GalNAc-T3, Hs00237084_m1; GalNAc-T4, Hs00236809_s1; GalNAc-T5, Hs00294826_m1; GalNAc-T6, Hs00200529_m1, GalNAc-T7, Hs00213624_m1; GalNAc-T8, Hs00213610_m1; GalNAc-T9, Hs00222357_m1; GalNAc-T10, Hs00213903_m1; GalNAc-T11, Hs00254410_m1; GalNAc-T12, Generated:Article Title: Transcriptomic Response to 1,25-Dihydroxyvitamin D in Human Fibroblasts with or without a Functional Vitamin D Receptor (VDR): Novel Target Genes and Insights into VDR Basal Transcriptional Activity Article Snippet: .. First-strand cDNA was generated using SuperScript III reverse transcriptase (Thermo Fisher) and quantitative PCR performed in a StepOnePlus Real-Time PCR System (Thermo Fisher) using the following inventoried TaqMan® assays (Thermo Fisher): CYP24A1 Hs00167999 _m1, TGFB2 Hs00234244_m1, RASSF2 Hs00248129_m1, CILP Hs00173647_m1, STC1 Hs00174970_m1, SPP1 Hs00959010_m1, GALNT12 Reverse Transcription:Article Title: Transcriptomic Response to 1,25-Dihydroxyvitamin D in Human Fibroblasts with or without a Functional Vitamin D Receptor (VDR): Novel Target Genes and Insights into VDR Basal Transcriptional Activity Article Snippet: .. First-strand cDNA was generated using SuperScript III reverse transcriptase (Thermo Fisher) and quantitative PCR performed in a StepOnePlus Real-Time PCR System (Thermo Fisher) using the following inventoried TaqMan® assays (Thermo Fisher): CYP24A1 Hs00167999 _m1, TGFB2 Hs00234244_m1, RASSF2 Hs00248129_m1, CILP Hs00173647_m1, STC1 Hs00174970_m1, SPP1 Hs00959010_m1, GALNT12 |