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human lncrna microarray nimblegen 12 × 135 k array platform  (Arraystar inc)

 
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    Arraystar inc human lncrna microarray nimblegen 12 × 135 k array platform
    Gene regulation in endothelial cells by STEEL RNA. (A) RT-qPCR of STEEL knockdown by two independent siRNA sequences. (B) Volcano plot representing mRNAs regulated by STEEL knockdown in HUVECs and HMVECs from <t>microarray.</t> Red indicates differentially regulated genes. (C–F) RT-qPCR of HOXD1 and HOXD3 mRNA with STEEL knockdown. (D) Western blot of HOXD1 and HOXD3 protein with STEEL knockdown. (E) RT-qPCR of KLF2 and eNOS mRNA with STEEL knockdown. (F) Western blot of KLF2 and eNOS protein with STEEL knockdown. (G) RT-qPCR of KLF2 and eNOS hnRNA (pre-mRNA) with STEEL knockdown. (H) RT-qPCR of RNA Pol II ChIP at KLF2 and eNOS promoters. (I) Promoter luciferase-reporter assays with STEEL knockdown. (J) CHART-qPCR with STEEL knockdown. (K and L) ChIP-qPCR of the core histone, histone H3, and the activating epigenetic mark histone 3 lysine 4 trimethyl (H3K4me3) at the KLF2 and eNOS promoters after STEEL siRNA knockdown in HUVECs. *P < 0.05. Error bars represent SEM.
    Human Lncrna Microarray Nimblegen 12 × 135 K Array Platform, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+human+1%2E2+cdna+microarray/pmc05877935-332-5-4?v=Arraystar+inc
    Average 90 stars, based on 1 article reviews
    human lncrna microarray nimblegen 12 × 135 k array platform - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Angiogenic patterning by STEEL, an endothelial-enriched long noncoding RNA"

    Article Title: Angiogenic patterning by STEEL, an endothelial-enriched long noncoding RNA

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.1715182115

    Gene regulation in endothelial cells by STEEL RNA. (A) RT-qPCR of STEEL knockdown by two independent siRNA sequences. (B) Volcano plot representing mRNAs regulated by STEEL knockdown in HUVECs and HMVECs from microarray. Red indicates differentially regulated genes. (C–F) RT-qPCR of HOXD1 and HOXD3 mRNA with STEEL knockdown. (D) Western blot of HOXD1 and HOXD3 protein with STEEL knockdown. (E) RT-qPCR of KLF2 and eNOS mRNA with STEEL knockdown. (F) Western blot of KLF2 and eNOS protein with STEEL knockdown. (G) RT-qPCR of KLF2 and eNOS hnRNA (pre-mRNA) with STEEL knockdown. (H) RT-qPCR of RNA Pol II ChIP at KLF2 and eNOS promoters. (I) Promoter luciferase-reporter assays with STEEL knockdown. (J) CHART-qPCR with STEEL knockdown. (K and L) ChIP-qPCR of the core histone, histone H3, and the activating epigenetic mark histone 3 lysine 4 trimethyl (H3K4me3) at the KLF2 and eNOS promoters after STEEL siRNA knockdown in HUVECs. *P < 0.05. Error bars represent SEM.
    Figure Legend Snippet: Gene regulation in endothelial cells by STEEL RNA. (A) RT-qPCR of STEEL knockdown by two independent siRNA sequences. (B) Volcano plot representing mRNAs regulated by STEEL knockdown in HUVECs and HMVECs from microarray. Red indicates differentially regulated genes. (C–F) RT-qPCR of HOXD1 and HOXD3 mRNA with STEEL knockdown. (D) Western blot of HOXD1 and HOXD3 protein with STEEL knockdown. (E) RT-qPCR of KLF2 and eNOS mRNA with STEEL knockdown. (F) Western blot of KLF2 and eNOS protein with STEEL knockdown. (G) RT-qPCR of KLF2 and eNOS hnRNA (pre-mRNA) with STEEL knockdown. (H) RT-qPCR of RNA Pol II ChIP at KLF2 and eNOS promoters. (I) Promoter luciferase-reporter assays with STEEL knockdown. (J) CHART-qPCR with STEEL knockdown. (K and L) ChIP-qPCR of the core histone, histone H3, and the activating epigenetic mark histone 3 lysine 4 trimethyl (H3K4me3) at the KLF2 and eNOS promoters after STEEL siRNA knockdown in HUVECs. *P < 0.05. Error bars represent SEM.

    Techniques Used: Quantitative RT-PCR, Microarray, Western Blot, Luciferase



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