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single component tmb substrate solution  (tiangen biotech co)


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    tiangen biotech co single component tmb substrate solution
    Single Component Tmb Substrate Solution, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 99/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tmb+single-component+substrate+solution/Soluble+TMB+Substrate+Solution/pm41548284-52-2-9
    Average 99 stars, based on 104 article reviews
    single component tmb substrate solution - by Bioz Stars, 2026-09
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    Related Articles

    Incubation:

    Article Title: FabG moonlights as an extracellular adhesin mediates cytoadhesion of Streptococcus suis via interaction with plasminogen
    Article Snippet: After blocking with 5% bovine serum albumin (BSA, Solarbio, Cat No. A8020), the plate was incubated with different concentrations of rFabG. .. After incubation with mouse anti-His-tag monoclonal antibody and HRP-conjugated goat anti-mouse IgG, the reaction was detected using TMB substrate (Tiangen, Cat No. PA107) for 15 min and stopped with 2M H 2 SO 4 , absorbance was measured at 450 nm. ..

    Article Title: FabG moonlights as an extracellular adhesin mediates cytoadhesion of Streptococcus suis via interaction with plasminogen.
    Article Snippet: After blocking with 5% bovine serum albumin (BSA, Solarbio, Cat No. A8020), the plate was incubated with different concentrations of rFabG. .. After incubation with mouse anti-His-tag monoclonal antibody and HRP-conjugated goat antimouse IgG, the reaction was detected using TMB substrate (Tiangen, Cat No. PA107) for 15 min and stopped with 2M H2SO4, absorbance was measured at 450 nm. ..

    Article Title: Self-assembling ferritin nanoplatform enables amyloid-β-targeted immunotherapy and cognitive rescue in APP/PS1 mice.
    Article Snippet: Alzheimer’s disease (AD) is a progressively neurodegenerative disorder.. The accumulation and pathological aggregation of amyloid-β (Aβ) peptides are key events in the occurrence and progression of AD, positioning Aβtargeted immunotherapy as a precision therapeutic approach.. We present a click chemistry-assisted modular nanoparticle vaccine, Fe-Spy-3CAβ, constructed via SpyCatcher-SpyTag-mediated site-specific conjugation of Aβ1-6 epitopes onto engineered ferritin nanocages.

    Activity Assay:

    Article Title: Self-assembling ferritin nanoplatform enables amyloid-β-targeted immunotherapy and cognitive rescue in APP/PS1 mice.
    Article Snippet: Alzheimer’s disease (AD) is a progressively neurodegenerative disorder.. The accumulation and pathological aggregation of amyloid-β (Aβ) peptides are key events in the occurrence and progression of AD, positioning Aβtargeted immunotherapy as a precision therapeutic approach.. We present a click chemistry-assisted modular nanoparticle vaccine, Fe-Spy-3CAβ, constructed via SpyCatcher-SpyTag-mediated site-specific conjugation of Aβ1-6 epitopes onto engineered ferritin nanocages.

    Recombinant:

    Article Title: A single-dose nanoparticle vaccine protects against mpox in preclinical models.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE-CyTM7 Hamster Anti-Mouse CD3e, clone 145-2C11 BD Pharmingen Cat#135210; RRID: AB_2159183 BV510 Rat Anti-Mouse CD4, clone RM4-5 BD Pharmingen Cat#563106; RRID:AB_2687550 PerCP-CyTM5.5 Rat Anti-Mouse CD8a, clone 53–6.7 BD Pharmingen Cat#551162; RRID:AB_394081 Brilliant Violet 421TM anti-mouse IL-4 Antibody, clone 11B11 BioLegend Cat#504120; RRID:AB_2562102 FITC Rat Anti-Mouse IFN-γ, clone XMG1.2 BD Pharmingen Cat#554411; RRID:AB_AB_395375 PE Rat Anti-Mouse TNF, clone MP6-XT22 BD Pharmingen Cat#554419; RRID:AB_395380 Purified Rat Anti-Mouse CD16/CD32, clone 2.4G2 BD Pharmingen Cat#553141; RRID:AB_394656 PerCP/Cyanine5.5 anti-mouse/human CD45R/B220, clone RA3-6B2 BioLegend Cat#103236; RRID:AB_893354 PE Rat anti-Mouse IgD, clone 11-26c.2a BD Pharmingen Cat#558597; RRID:AB_647211 Alexa Fluor® 647 Rat Anti-Mouse T- and B-Cell Activation Antigen, Clone GL7 BD Pharmingen Cat#561529; RRID:AB_10716056 FITC anti-mouse CD95 (Fas) Antibody, clone SA367H8 BioLegend Cat#152606; RRID:AB_2632901 PE anti-mouse CD185 (CXCR5) antibody, clone SPRCL5 eBioscience Cat#12-7185-82; RRID: AB_11217882 APC anti-mouse CD279 (PD-1) Antibody, Clone 29F.1A12 BioLegend Cat#135210; RRID:AB_2159183 Goat Anti-Mouse IgG H&L (HRP) Abcam Cat#ab6789; RRID:AB_955439 Goat Anti-Mouse IgG1 (HRP) Abcam Cat#ab97240; RRID: AB_10695944 Goat Anti-Mouse IgG2a (HRP) Abcam Cat#ab97245; RRID:AB_10680049 Anti-Rabbit IgG (H + L) (HRP) Invitrogen Cat#A16096; RRID:AB_2534770 Goat Anti-Human IgG - H&L (HRP) Abcam Cat#ab6858; RRID:AB_955433 7D11 This paper N/A A27D7 This paper N/A M1H11 Ren et al.51 N/A Bacterial and virus strains DH5α competent cells AlpaLifeBio Cat#KTSM101L Rosetta (DE3) competent cells Tiangen Cat#CB108-02 VACV Tiantan strain Zhang et al.75 N/A VTT-GFP strain Ren et al.51 N/A VACV Western Reserve strain Zuo et al.67 N/A Clade I mpox virus This paper N/A Clade II mpox virus(hMpxV/China/SZSZTH41/2023) Cheng et al.68 N/A Biological samples Serum samples from immunized BALB/c mice This paper N/A Serum samples from immunized New Zealand rabbits This paper N/A MPXV human convalescent sera This paper N/A (Continued on next page) Cell Reports 44, 116358, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins FH002C adjuvant Wu et al.52 N/A Sulfo-NHS-LC-LC-biotin Thermo Fisher Scientific Cat#21338 TMB substrate Tiangen CatPA107-02 BamHI-HF NEB Cat#R3136L NdeI NEB Cat#R0111L XhoI NEB Cat#R0146M Coomassie Brilliant Blue R250 Meilunbio Cat#MB4685 Bovine serum albumin V (BSA V) Biofroxx Cat#4240GR500 Tween 20 Biofroxx Cat#1247ML500 Trehalose Bioss Cat#D10226 TritonTM X-114 Sigma-Aldrich Cat#X114-100ML 2 × Phanta Max Master Mix Vazyme Cat#P515-03 PEI-MAX Polyscience Cat#24765-1 ELISA coated solution (1x) Solarbio Cat#C1050 ELISA Stop Solution Solarbio Cat#C1058 4% polyformaldehyde Yuanye Cat#R20497 Avicel FMC biopolymer Cat#RC-591 10×ACK lysis buffer Elabscience Cat#E-CK-A105 BD GolgiPlugTM Protein Transport Inhibitor BD Pharmingen Cat#555029; RRID: AB_2869014 Leukocyte Activation Cocktail BD Pharmingen Cat#550583; RRID: AB_2868893 Dithiothreitol (DTT) Thermo Fisher Scientific Cat#A39255 Isopropyl b-D-1 thiogalactopyranoside (IPTG) Takara Cat#9030 Standard protein marker Thermo Fisher Scientific Cat#26616 Recombinant A35 protein This paper N/A Recombinant M1 protein This paper N/A Recombinant GvO-DAM protein This paper N/A Recombinant Sd-Ferritin protein This paper N/A Recombinant DAM protein This paper N/A Recombinant A29 protein This paper N/A Recombinant B6 protein This paper N/A Recombinant E8 protein This paper N/A Recombinant Ferritin protein This paper N/A Critical commercial assays Plasmid extraction kit Macherey-Nagel Cat#740412.50 ClonExpress Ultra One Step Cloning Kit Vazyme Cat#C117-02 BCA protein assay kit Thermo Fisher Scientific Cat#A55864 FastPure Viral DNA/RNA Mini Kit Pro Vazyme Cat#RC323-01 2xChamQ Universal SYBR qPCR Master Mix Vazyme Cat#Q711-02 Cytofix/CytopermTM Fixation/ Permeabilization Kit BD Bioscience Cat#554714; RRID: AB_2869008 ToxinSensor Chromogenic LAL Endotoxin Assay Kit GenScript Cat#L00350 Experimental models: Cell lines Expi 293F Thermo Fisher Scientific Cat#A14527 Vero E6 ATCC Cat#CRL1586 (Continued on next page) 18 Cell Reports 44, 116358, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Balb/C mice Guangdong Medical Laboratory Animal Center N/A New Zealand rabbit Guangdong Medical Laboratory Animal Center N/A Oligonucleotides VACV-F 5′-ACATCTGGAGAATCCACAACA-3′ Ren et al.51 N/A VACV-R 5′-CATCATCGGTGGTTGATTTA-3′ Ren et al.51 N/A VACV HA TaqMan probe 5′- FAM-GAGACTCCGGAACCAATTAMRA-3′ Ren et al.51 N/A Recombinant DNA VRC8400-M1R-his This paper N/A VRC8400-B6R-his This paper N/A VRC8400-GVO-DAM-his This paper N/A VRC8400-DAM-his This paper N/A pTO-T7-Sd-Ferritin This paper N/A pTO-T7-Ferritin This paper N/A pTO-T7-A35R-his This paper N/A pTO-T7-A29L-his This paper N/A pTO-T7-E8L-his This paper N/A pCAGGS-7D11(VH)-IgG1 This paper N/A pCAGGS-7D11(VL)-IgG1 This paper N/A pCAGGS-A27D7(VH)-IgG2a This paper N/A pCAGGS-A27D7(VL)-IgG2a This paper N/A Software and algorithms GraphPad Prism V9.5.1 Graphpad https://www.graphpad.com/ SlideViewer 3DHISTECH Ltd. https://www.3dhistech.com/ Tanon 1600 automatic Digital Gel Image Analysis system Tanon https://tanon.cnreagent.com/ Zetasizer Ultra ZS Xplorer V2.0 Malvern Panalytical https://www.malvernpanalytical.com/ PyMol The PyMOL Molecular Graphics System, Version 2.0 Schrö dinger, LLC. https://pymol.org/ FlowJo V10 FlowJo https://www.flowjo.com Octet BLI Analysis 12.2 Fortebio N/A Other Expi 293F serum-free medium OPM Biosciences Cat#AC601501-1000mL Protein A Resin GenScript Cat#L00210-50 Protein G Resin GenScript Cat#L00209-50 Filter paper Cytiva Cat#1004047 Superdex 200 Increase 10/300 GL column Cytiva Cat#28990944 Superose 6 Increase 10/300 GL column Cytiva Cat#29091596 HiLoad 26/600 Superdex 200 pg SEC column Cytiva Cat#28989336 Ni Sepharose excel resin Cytiva Cat#17371203 HiScreen Capto Core 700 column Cytiva Cat#17548115 Capto DEAE column Cytiva Cat#17544310 10K MWCO ultracentrifuge tube Merck millipore Cat#UFC901096 (Continued on next page) Cell Reports 44, 116358, October 28, 2025 19

    Adjuvant:

    Article Title: A single-dose nanoparticle vaccine protects against mpox in preclinical models.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE-CyTM7 Hamster Anti-Mouse CD3e, clone 145-2C11 BD Pharmingen Cat#135210; RRID: AB_2159183 BV510 Rat Anti-Mouse CD4, clone RM4-5 BD Pharmingen Cat#563106; RRID:AB_2687550 PerCP-CyTM5.5 Rat Anti-Mouse CD8a, clone 53–6.7 BD Pharmingen Cat#551162; RRID:AB_394081 Brilliant Violet 421TM anti-mouse IL-4 Antibody, clone 11B11 BioLegend Cat#504120; RRID:AB_2562102 FITC Rat Anti-Mouse IFN-γ, clone XMG1.2 BD Pharmingen Cat#554411; RRID:AB_AB_395375 PE Rat Anti-Mouse TNF, clone MP6-XT22 BD Pharmingen Cat#554419; RRID:AB_395380 Purified Rat Anti-Mouse CD16/CD32, clone 2.4G2 BD Pharmingen Cat#553141; RRID:AB_394656 PerCP/Cyanine5.5 anti-mouse/human CD45R/B220, clone RA3-6B2 BioLegend Cat#103236; RRID:AB_893354 PE Rat anti-Mouse IgD, clone 11-26c.2a BD Pharmingen Cat#558597; RRID:AB_647211 Alexa Fluor® 647 Rat Anti-Mouse T- and B-Cell Activation Antigen, Clone GL7 BD Pharmingen Cat#561529; RRID:AB_10716056 FITC anti-mouse CD95 (Fas) Antibody, clone SA367H8 BioLegend Cat#152606; RRID:AB_2632901 PE anti-mouse CD185 (CXCR5) antibody, clone SPRCL5 eBioscience Cat#12-7185-82; RRID: AB_11217882 APC anti-mouse CD279 (PD-1) Antibody, Clone 29F.1A12 BioLegend Cat#135210; RRID:AB_2159183 Goat Anti-Mouse IgG H&L (HRP) Abcam Cat#ab6789; RRID:AB_955439 Goat Anti-Mouse IgG1 (HRP) Abcam Cat#ab97240; RRID: AB_10695944 Goat Anti-Mouse IgG2a (HRP) Abcam Cat#ab97245; RRID:AB_10680049 Anti-Rabbit IgG (H + L) (HRP) Invitrogen Cat#A16096; RRID:AB_2534770 Goat Anti-Human IgG - H&L (HRP) Abcam Cat#ab6858; RRID:AB_955433 7D11 This paper N/A A27D7 This paper N/A M1H11 Ren et al.51 N/A Bacterial and virus strains DH5α competent cells AlpaLifeBio Cat#KTSM101L Rosetta (DE3) competent cells Tiangen Cat#CB108-02 VACV Tiantan strain Zhang et al.75 N/A VTT-GFP strain Ren et al.51 N/A VACV Western Reserve strain Zuo et al.67 N/A Clade I mpox virus This paper N/A Clade II mpox virus(hMpxV/China/SZSZTH41/2023) Cheng et al.68 N/A Biological samples Serum samples from immunized BALB/c mice This paper N/A Serum samples from immunized New Zealand rabbits This paper N/A MPXV human convalescent sera This paper N/A (Continued on next page) Cell Reports 44, 116358, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins FH002C adjuvant Wu et al.52 N/A Sulfo-NHS-LC-LC-biotin Thermo Fisher Scientific Cat#21338 TMB substrate Tiangen CatPA107-02 BamHI-HF NEB Cat#R3136L NdeI NEB Cat#R0111L XhoI NEB Cat#R0146M Coomassie Brilliant Blue R250 Meilunbio Cat#MB4685 Bovine serum albumin V (BSA V) Biofroxx Cat#4240GR500 Tween 20 Biofroxx Cat#1247ML500 Trehalose Bioss Cat#D10226 TritonTM X-114 Sigma-Aldrich Cat#X114-100ML 2 × Phanta Max Master Mix Vazyme Cat#P515-03 PEI-MAX Polyscience Cat#24765-1 ELISA coated solution (1x) Solarbio Cat#C1050 ELISA Stop Solution Solarbio Cat#C1058 4% polyformaldehyde Yuanye Cat#R20497 Avicel FMC biopolymer Cat#RC-591 10×ACK lysis buffer Elabscience Cat#E-CK-A105 BD GolgiPlugTM Protein Transport Inhibitor BD Pharmingen Cat#555029; RRID: AB_2869014 Leukocyte Activation Cocktail BD Pharmingen Cat#550583; RRID: AB_2868893 Dithiothreitol (DTT) Thermo Fisher Scientific Cat#A39255 Isopropyl b-D-1 thiogalactopyranoside (IPTG) Takara Cat#9030 Standard protein marker Thermo Fisher Scientific Cat#26616 Recombinant A35 protein This paper N/A Recombinant M1 protein This paper N/A Recombinant GvO-DAM protein This paper N/A Recombinant Sd-Ferritin protein This paper N/A Recombinant DAM protein This paper N/A Recombinant A29 protein This paper N/A Recombinant B6 protein This paper N/A Recombinant E8 protein This paper N/A Recombinant Ferritin protein This paper N/A Critical commercial assays Plasmid extraction kit Macherey-Nagel Cat#740412.50 ClonExpress Ultra One Step Cloning Kit Vazyme Cat#C117-02 BCA protein assay kit Thermo Fisher Scientific Cat#A55864 FastPure Viral DNA/RNA Mini Kit Pro Vazyme Cat#RC323-01 2xChamQ Universal SYBR qPCR Master Mix Vazyme Cat#Q711-02 Cytofix/CytopermTM Fixation/ Permeabilization Kit BD Bioscience Cat#554714; RRID: AB_2869008 ToxinSensor Chromogenic LAL Endotoxin Assay Kit GenScript Cat#L00350 Experimental models: Cell lines Expi 293F Thermo Fisher Scientific Cat#A14527 Vero E6 ATCC Cat#CRL1586 (Continued on next page) 18 Cell Reports 44, 116358, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Balb/C mice Guangdong Medical Laboratory Animal Center N/A New Zealand rabbit Guangdong Medical Laboratory Animal Center N/A Oligonucleotides VACV-F 5′-ACATCTGGAGAATCCACAACA-3′ Ren et al.51 N/A VACV-R 5′-CATCATCGGTGGTTGATTTA-3′ Ren et al.51 N/A VACV HA TaqMan probe 5′- FAM-GAGACTCCGGAACCAATTAMRA-3′ Ren et al.51 N/A Recombinant DNA VRC8400-M1R-his This paper N/A VRC8400-B6R-his This paper N/A VRC8400-GVO-DAM-his This paper N/A VRC8400-DAM-his This paper N/A pTO-T7-Sd-Ferritin This paper N/A pTO-T7-Ferritin This paper N/A pTO-T7-A35R-his This paper N/A pTO-T7-A29L-his This paper N/A pTO-T7-E8L-his This paper N/A pCAGGS-7D11(VH)-IgG1 This paper N/A pCAGGS-7D11(VL)-IgG1 This paper N/A pCAGGS-A27D7(VH)-IgG2a This paper N/A pCAGGS-A27D7(VL)-IgG2a This paper N/A Software and algorithms GraphPad Prism V9.5.1 Graphpad https://www.graphpad.com/ SlideViewer 3DHISTECH Ltd. https://www.3dhistech.com/ Tanon 1600 automatic Digital Gel Image Analysis system Tanon https://tanon.cnreagent.com/ Zetasizer Ultra ZS Xplorer V2.0 Malvern Panalytical https://www.malvernpanalytical.com/ PyMol The PyMOL Molecular Graphics System, Version 2.0 Schrö dinger, LLC. https://pymol.org/ FlowJo V10 FlowJo https://www.flowjo.com Octet BLI Analysis 12.2 Fortebio N/A Other Expi 293F serum-free medium OPM Biosciences Cat#AC601501-1000mL Protein A Resin GenScript Cat#L00210-50 Protein G Resin GenScript Cat#L00209-50 Filter paper Cytiva Cat#1004047 Superdex 200 Increase 10/300 GL column Cytiva Cat#28990944 Superose 6 Increase 10/300 GL column Cytiva Cat#29091596 HiLoad 26/600 Superdex 200 pg SEC column Cytiva Cat#28989336 Ni Sepharose excel resin Cytiva Cat#17371203 HiScreen Capto Core 700 column Cytiva Cat#17548115 Capto DEAE column Cytiva Cat#17544310 10K MWCO ultracentrifuge tube Merck millipore Cat#UFC901096 (Continued on next page) Cell Reports 44, 116358, October 28, 2025 19

    Enzyme-linked Immunosorbent Assay:

    Article Title: A single-dose nanoparticle vaccine protects against mpox in preclinical models.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE-CyTM7 Hamster Anti-Mouse CD3e, clone 145-2C11 BD Pharmingen Cat#135210; RRID: AB_2159183 BV510 Rat Anti-Mouse CD4, clone RM4-5 BD Pharmingen Cat#563106; RRID:AB_2687550 PerCP-CyTM5.5 Rat Anti-Mouse CD8a, clone 53–6.7 BD Pharmingen Cat#551162; RRID:AB_394081 Brilliant Violet 421TM anti-mouse IL-4 Antibody, clone 11B11 BioLegend Cat#504120; RRID:AB_2562102 FITC Rat Anti-Mouse IFN-γ, clone XMG1.2 BD Pharmingen Cat#554411; RRID:AB_AB_395375 PE Rat Anti-Mouse TNF, clone MP6-XT22 BD Pharmingen Cat#554419; RRID:AB_395380 Purified Rat Anti-Mouse CD16/CD32, clone 2.4G2 BD Pharmingen Cat#553141; RRID:AB_394656 PerCP/Cyanine5.5 anti-mouse/human CD45R/B220, clone RA3-6B2 BioLegend Cat#103236; RRID:AB_893354 PE Rat anti-Mouse IgD, clone 11-26c.2a BD Pharmingen Cat#558597; RRID:AB_647211 Alexa Fluor® 647 Rat Anti-Mouse T- and B-Cell Activation Antigen, Clone GL7 BD Pharmingen Cat#561529; RRID:AB_10716056 FITC anti-mouse CD95 (Fas) Antibody, clone SA367H8 BioLegend Cat#152606; RRID:AB_2632901 PE anti-mouse CD185 (CXCR5) antibody, clone SPRCL5 eBioscience Cat#12-7185-82; RRID: AB_11217882 APC anti-mouse CD279 (PD-1) Antibody, Clone 29F.1A12 BioLegend Cat#135210; RRID:AB_2159183 Goat Anti-Mouse IgG H&L (HRP) Abcam Cat#ab6789; RRID:AB_955439 Goat Anti-Mouse IgG1 (HRP) Abcam Cat#ab97240; RRID: AB_10695944 Goat Anti-Mouse IgG2a (HRP) Abcam Cat#ab97245; RRID:AB_10680049 Anti-Rabbit IgG (H + L) (HRP) Invitrogen Cat#A16096; RRID:AB_2534770 Goat Anti-Human IgG - H&L (HRP) Abcam Cat#ab6858; RRID:AB_955433 7D11 This paper N/A A27D7 This paper N/A M1H11 Ren et al.51 N/A Bacterial and virus strains DH5α competent cells AlpaLifeBio Cat#KTSM101L Rosetta (DE3) competent cells Tiangen Cat#CB108-02 VACV Tiantan strain Zhang et al.75 N/A VTT-GFP strain Ren et al.51 N/A VACV Western Reserve strain Zuo et al.67 N/A Clade I mpox virus This paper N/A Clade II mpox virus(hMpxV/China/SZSZTH41/2023) Cheng et al.68 N/A Biological samples Serum samples from immunized BALB/c mice This paper N/A Serum samples from immunized New Zealand rabbits This paper N/A MPXV human convalescent sera This paper N/A (Continued on next page) Cell Reports 44, 116358, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins FH002C adjuvant Wu et al.52 N/A Sulfo-NHS-LC-LC-biotin Thermo Fisher Scientific Cat#21338 TMB substrate Tiangen CatPA107-02 BamHI-HF NEB Cat#R3136L NdeI NEB Cat#R0111L XhoI NEB Cat#R0146M Coomassie Brilliant Blue R250 Meilunbio Cat#MB4685 Bovine serum albumin V (BSA V) Biofroxx Cat#4240GR500 Tween 20 Biofroxx Cat#1247ML500 Trehalose Bioss Cat#D10226 TritonTM X-114 Sigma-Aldrich Cat#X114-100ML 2 × Phanta Max Master Mix Vazyme Cat#P515-03 PEI-MAX Polyscience Cat#24765-1 ELISA coated solution (1x) Solarbio Cat#C1050 ELISA Stop Solution Solarbio Cat#C1058 4% polyformaldehyde Yuanye Cat#R20497 Avicel FMC biopolymer Cat#RC-591 10×ACK lysis buffer Elabscience Cat#E-CK-A105 BD GolgiPlugTM Protein Transport Inhibitor BD Pharmingen Cat#555029; RRID: AB_2869014 Leukocyte Activation Cocktail BD Pharmingen Cat#550583; RRID: AB_2868893 Dithiothreitol (DTT) Thermo Fisher Scientific Cat#A39255 Isopropyl b-D-1 thiogalactopyranoside (IPTG) Takara Cat#9030 Standard protein marker Thermo Fisher Scientific Cat#26616 Recombinant A35 protein This paper N/A Recombinant M1 protein This paper N/A Recombinant GvO-DAM protein This paper N/A Recombinant Sd-Ferritin protein This paper N/A Recombinant DAM protein This paper N/A Recombinant A29 protein This paper N/A Recombinant B6 protein This paper N/A Recombinant E8 protein This paper N/A Recombinant Ferritin protein This paper N/A Critical commercial assays Plasmid extraction kit Macherey-Nagel Cat#740412.50 ClonExpress Ultra One Step Cloning Kit Vazyme Cat#C117-02 BCA protein assay kit Thermo Fisher Scientific Cat#A55864 FastPure Viral DNA/RNA Mini Kit Pro Vazyme Cat#RC323-01 2xChamQ Universal SYBR qPCR Master Mix Vazyme Cat#Q711-02 Cytofix/CytopermTM Fixation/ Permeabilization Kit BD Bioscience Cat#554714; RRID: AB_2869008 ToxinSensor Chromogenic LAL Endotoxin Assay Kit GenScript Cat#L00350 Experimental models: Cell lines Expi 293F Thermo Fisher Scientific Cat#A14527 Vero E6 ATCC Cat#CRL1586 (Continued on next page) 18 Cell Reports 44, 116358, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Balb/C mice Guangdong Medical Laboratory Animal Center N/A New Zealand rabbit Guangdong Medical Laboratory Animal Center N/A Oligonucleotides VACV-F 5′-ACATCTGGAGAATCCACAACA-3′ Ren et al.51 N/A VACV-R 5′-CATCATCGGTGGTTGATTTA-3′ Ren et al.51 N/A VACV HA TaqMan probe 5′- FAM-GAGACTCCGGAACCAATTAMRA-3′ Ren et al.51 N/A Recombinant DNA VRC8400-M1R-his This paper N/A VRC8400-B6R-his This paper N/A VRC8400-GVO-DAM-his This paper N/A VRC8400-DAM-his This paper N/A pTO-T7-Sd-Ferritin This paper N/A pTO-T7-Ferritin This paper N/A pTO-T7-A35R-his This paper N/A pTO-T7-A29L-his This paper N/A pTO-T7-E8L-his This paper N/A pCAGGS-7D11(VH)-IgG1 This paper N/A pCAGGS-7D11(VL)-IgG1 This paper N/A pCAGGS-A27D7(VH)-IgG2a This paper N/A pCAGGS-A27D7(VL)-IgG2a This paper N/A Software and algorithms GraphPad Prism V9.5.1 Graphpad https://www.graphpad.com/ SlideViewer 3DHISTECH Ltd. https://www.3dhistech.com/ Tanon 1600 automatic Digital Gel Image Analysis system Tanon https://tanon.cnreagent.com/ Zetasizer Ultra ZS Xplorer V2.0 Malvern Panalytical https://www.malvernpanalytical.com/ PyMol The PyMOL Molecular Graphics System, Version 2.0 Schrö dinger, LLC. https://pymol.org/ FlowJo V10 FlowJo https://www.flowjo.com Octet BLI Analysis 12.2 Fortebio N/A Other Expi 293F serum-free medium OPM Biosciences Cat#AC601501-1000mL Protein A Resin GenScript Cat#L00210-50 Protein G Resin GenScript Cat#L00209-50 Filter paper Cytiva Cat#1004047 Superdex 200 Increase 10/300 GL column Cytiva Cat#28990944 Superose 6 Increase 10/300 GL column Cytiva Cat#29091596 HiLoad 26/600 Superdex 200 pg SEC column Cytiva Cat#28989336 Ni Sepharose excel resin Cytiva Cat#17371203 HiScreen Capto Core 700 column Cytiva Cat#17548115 Capto DEAE column Cytiva Cat#17544310 10K MWCO ultracentrifuge tube Merck millipore Cat#UFC901096 (Continued on next page) Cell Reports 44, 116358, October 28, 2025 19

    Lysis:

    Article Title: A single-dose nanoparticle vaccine protects against mpox in preclinical models.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE-CyTM7 Hamster Anti-Mouse CD3e, clone 145-2C11 BD Pharmingen Cat#135210; RRID: AB_2159183 BV510 Rat Anti-Mouse CD4, clone RM4-5 BD Pharmingen Cat#563106; RRID:AB_2687550 PerCP-CyTM5.5 Rat Anti-Mouse CD8a, clone 53–6.7 BD Pharmingen Cat#551162; RRID:AB_394081 Brilliant Violet 421TM anti-mouse IL-4 Antibody, clone 11B11 BioLegend Cat#504120; RRID:AB_2562102 FITC Rat Anti-Mouse IFN-γ, clone XMG1.2 BD Pharmingen Cat#554411; RRID:AB_AB_395375 PE Rat Anti-Mouse TNF, clone MP6-XT22 BD Pharmingen Cat#554419; RRID:AB_395380 Purified Rat Anti-Mouse CD16/CD32, clone 2.4G2 BD Pharmingen Cat#553141; RRID:AB_394656 PerCP/Cyanine5.5 anti-mouse/human CD45R/B220, clone RA3-6B2 BioLegend Cat#103236; RRID:AB_893354 PE Rat anti-Mouse IgD, clone 11-26c.2a BD Pharmingen Cat#558597; RRID:AB_647211 Alexa Fluor® 647 Rat Anti-Mouse T- and B-Cell Activation Antigen, Clone GL7 BD Pharmingen Cat#561529; RRID:AB_10716056 FITC anti-mouse CD95 (Fas) Antibody, clone SA367H8 BioLegend Cat#152606; RRID:AB_2632901 PE anti-mouse CD185 (CXCR5) antibody, clone SPRCL5 eBioscience Cat#12-7185-82; RRID: AB_11217882 APC anti-mouse CD279 (PD-1) Antibody, Clone 29F.1A12 BioLegend Cat#135210; RRID:AB_2159183 Goat Anti-Mouse IgG H&L (HRP) Abcam Cat#ab6789; RRID:AB_955439 Goat Anti-Mouse IgG1 (HRP) Abcam Cat#ab97240; RRID: AB_10695944 Goat Anti-Mouse IgG2a (HRP) Abcam Cat#ab97245; RRID:AB_10680049 Anti-Rabbit IgG (H + L) (HRP) Invitrogen Cat#A16096; RRID:AB_2534770 Goat Anti-Human IgG - H&L (HRP) Abcam Cat#ab6858; RRID:AB_955433 7D11 This paper N/A A27D7 This paper N/A M1H11 Ren et al.51 N/A Bacterial and virus strains DH5α competent cells AlpaLifeBio Cat#KTSM101L Rosetta (DE3) competent cells Tiangen Cat#CB108-02 VACV Tiantan strain Zhang et al.75 N/A VTT-GFP strain Ren et al.51 N/A VACV Western Reserve strain Zuo et al.67 N/A Clade I mpox virus This paper N/A Clade II mpox virus(hMpxV/China/SZSZTH41/2023) Cheng et al.68 N/A Biological samples Serum samples from immunized BALB/c mice This paper N/A Serum samples from immunized New Zealand rabbits This paper N/A MPXV human convalescent sera This paper N/A (Continued on next page) Cell Reports 44, 116358, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins FH002C adjuvant Wu et al.52 N/A Sulfo-NHS-LC-LC-biotin Thermo Fisher Scientific Cat#21338 TMB substrate Tiangen CatPA107-02 BamHI-HF NEB Cat#R3136L NdeI NEB Cat#R0111L XhoI NEB Cat#R0146M Coomassie Brilliant Blue R250 Meilunbio Cat#MB4685 Bovine serum albumin V (BSA V) Biofroxx Cat#4240GR500 Tween 20 Biofroxx Cat#1247ML500 Trehalose Bioss Cat#D10226 TritonTM X-114 Sigma-Aldrich Cat#X114-100ML 2 × Phanta Max Master Mix Vazyme Cat#P515-03 PEI-MAX Polyscience Cat#24765-1 ELISA coated solution (1x) Solarbio Cat#C1050 ELISA Stop Solution Solarbio Cat#C1058 4% polyformaldehyde Yuanye Cat#R20497 Avicel FMC biopolymer Cat#RC-591 10×ACK lysis buffer Elabscience Cat#E-CK-A105 BD GolgiPlugTM Protein Transport Inhibitor BD Pharmingen Cat#555029; RRID: AB_2869014 Leukocyte Activation Cocktail BD Pharmingen Cat#550583; RRID: AB_2868893 Dithiothreitol (DTT) Thermo Fisher Scientific Cat#A39255 Isopropyl b-D-1 thiogalactopyranoside (IPTG) Takara Cat#9030 Standard protein marker Thermo Fisher Scientific Cat#26616 Recombinant A35 protein This paper N/A Recombinant M1 protein This paper N/A Recombinant GvO-DAM protein This paper N/A Recombinant Sd-Ferritin protein This paper N/A Recombinant DAM protein This paper N/A Recombinant A29 protein This paper N/A Recombinant B6 protein This paper N/A Recombinant E8 protein This paper N/A Recombinant Ferritin protein This paper N/A Critical commercial assays Plasmid extraction kit Macherey-Nagel Cat#740412.50 ClonExpress Ultra One Step Cloning Kit Vazyme Cat#C117-02 BCA protein assay kit Thermo Fisher Scientific Cat#A55864 FastPure Viral DNA/RNA Mini Kit Pro Vazyme Cat#RC323-01 2xChamQ Universal SYBR qPCR Master Mix Vazyme Cat#Q711-02 Cytofix/CytopermTM Fixation/ Permeabilization Kit BD Bioscience Cat#554714; RRID: AB_2869008 ToxinSensor Chromogenic LAL Endotoxin Assay Kit GenScript Cat#L00350 Experimental models: Cell lines Expi 293F Thermo Fisher Scientific Cat#A14527 Vero E6 ATCC Cat#CRL1586 (Continued on next page) 18 Cell Reports 44, 116358, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Balb/C mice Guangdong Medical Laboratory Animal Center N/A New Zealand rabbit Guangdong Medical Laboratory Animal Center N/A Oligonucleotides VACV-F 5′-ACATCTGGAGAATCCACAACA-3′ Ren et al.51 N/A VACV-R 5′-CATCATCGGTGGTTGATTTA-3′ Ren et al.51 N/A VACV HA TaqMan probe 5′- FAM-GAGACTCCGGAACCAATTAMRA-3′ Ren et al.51 N/A Recombinant DNA VRC8400-M1R-his This paper N/A VRC8400-B6R-his This paper N/A VRC8400-GVO-DAM-his This paper N/A VRC8400-DAM-his This paper N/A pTO-T7-Sd-Ferritin This paper N/A pTO-T7-Ferritin This paper N/A pTO-T7-A35R-his This paper N/A pTO-T7-A29L-his This paper N/A pTO-T7-E8L-his This paper N/A pCAGGS-7D11(VH)-IgG1 This paper N/A pCAGGS-7D11(VL)-IgG1 This paper N/A pCAGGS-A27D7(VH)-IgG2a This paper N/A pCAGGS-A27D7(VL)-IgG2a This paper N/A Software and algorithms GraphPad Prism V9.5.1 Graphpad https://www.graphpad.com/ SlideViewer 3DHISTECH Ltd. https://www.3dhistech.com/ Tanon 1600 automatic Digital Gel Image Analysis system Tanon https://tanon.cnreagent.com/ Zetasizer Ultra ZS Xplorer V2.0 Malvern Panalytical https://www.malvernpanalytical.com/ PyMol The PyMOL Molecular Graphics System, Version 2.0 Schrö dinger, LLC. https://pymol.org/ FlowJo V10 FlowJo https://www.flowjo.com Octet BLI Analysis 12.2 Fortebio N/A Other Expi 293F serum-free medium OPM Biosciences Cat#AC601501-1000mL Protein A Resin GenScript Cat#L00210-50 Protein G Resin GenScript Cat#L00209-50 Filter paper Cytiva Cat#1004047 Superdex 200 Increase 10/300 GL column Cytiva Cat#28990944 Superose 6 Increase 10/300 GL column Cytiva Cat#29091596 HiLoad 26/600 Superdex 200 pg SEC column Cytiva Cat#28989336 Ni Sepharose excel resin Cytiva Cat#17371203 HiScreen Capto Core 700 column Cytiva Cat#17548115 Capto DEAE column Cytiva Cat#17544310 10K MWCO ultracentrifuge tube Merck millipore Cat#UFC901096 (Continued on next page) Cell Reports 44, 116358, October 28, 2025 19

    Activation Assay:

    Article Title: A single-dose nanoparticle vaccine protects against mpox in preclinical models.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE-CyTM7 Hamster Anti-Mouse CD3e, clone 145-2C11 BD Pharmingen Cat#135210; RRID: AB_2159183 BV510 Rat Anti-Mouse CD4, clone RM4-5 BD Pharmingen Cat#563106; RRID:AB_2687550 PerCP-CyTM5.5 Rat Anti-Mouse CD8a, clone 53–6.7 BD Pharmingen Cat#551162; RRID:AB_394081 Brilliant Violet 421TM anti-mouse IL-4 Antibody, clone 11B11 BioLegend Cat#504120; RRID:AB_2562102 FITC Rat Anti-Mouse IFN-γ, clone XMG1.2 BD Pharmingen Cat#554411; RRID:AB_AB_395375 PE Rat Anti-Mouse TNF, clone MP6-XT22 BD Pharmingen Cat#554419; RRID:AB_395380 Purified Rat Anti-Mouse CD16/CD32, clone 2.4G2 BD Pharmingen Cat#553141; RRID:AB_394656 PerCP/Cyanine5.5 anti-mouse/human CD45R/B220, clone RA3-6B2 BioLegend Cat#103236; RRID:AB_893354 PE Rat anti-Mouse IgD, clone 11-26c.2a BD Pharmingen Cat#558597; RRID:AB_647211 Alexa Fluor® 647 Rat Anti-Mouse T- and B-Cell Activation Antigen, Clone GL7 BD Pharmingen Cat#561529; RRID:AB_10716056 FITC anti-mouse CD95 (Fas) Antibody, clone SA367H8 BioLegend Cat#152606; RRID:AB_2632901 PE anti-mouse CD185 (CXCR5) antibody, clone SPRCL5 eBioscience Cat#12-7185-82; RRID: AB_11217882 APC anti-mouse CD279 (PD-1) Antibody, Clone 29F.1A12 BioLegend Cat#135210; RRID:AB_2159183 Goat Anti-Mouse IgG H&L (HRP) Abcam Cat#ab6789; RRID:AB_955439 Goat Anti-Mouse IgG1 (HRP) Abcam Cat#ab97240; RRID: AB_10695944 Goat Anti-Mouse IgG2a (HRP) Abcam Cat#ab97245; RRID:AB_10680049 Anti-Rabbit IgG (H + L) (HRP) Invitrogen Cat#A16096; RRID:AB_2534770 Goat Anti-Human IgG - H&L (HRP) Abcam Cat#ab6858; RRID:AB_955433 7D11 This paper N/A A27D7 This paper N/A M1H11 Ren et al.51 N/A Bacterial and virus strains DH5α competent cells AlpaLifeBio Cat#KTSM101L Rosetta (DE3) competent cells Tiangen Cat#CB108-02 VACV Tiantan strain Zhang et al.75 N/A VTT-GFP strain Ren et al.51 N/A VACV Western Reserve strain Zuo et al.67 N/A Clade I mpox virus This paper N/A Clade II mpox virus(hMpxV/China/SZSZTH41/2023) Cheng et al.68 N/A Biological samples Serum samples from immunized BALB/c mice This paper N/A Serum samples from immunized New Zealand rabbits This paper N/A MPXV human convalescent sera This paper N/A (Continued on next page) Cell Reports 44, 116358, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins FH002C adjuvant Wu et al.52 N/A Sulfo-NHS-LC-LC-biotin Thermo Fisher Scientific Cat#21338 TMB substrate Tiangen CatPA107-02 BamHI-HF NEB Cat#R3136L NdeI NEB Cat#R0111L XhoI NEB Cat#R0146M Coomassie Brilliant Blue R250 Meilunbio Cat#MB4685 Bovine serum albumin V (BSA V) Biofroxx Cat#4240GR500 Tween 20 Biofroxx Cat#1247ML500 Trehalose Bioss Cat#D10226 TritonTM X-114 Sigma-Aldrich Cat#X114-100ML 2 × Phanta Max Master Mix Vazyme Cat#P515-03 PEI-MAX Polyscience Cat#24765-1 ELISA coated solution (1x) Solarbio Cat#C1050 ELISA Stop Solution Solarbio Cat#C1058 4% polyformaldehyde Yuanye Cat#R20497 Avicel FMC biopolymer Cat#RC-591 10×ACK lysis buffer Elabscience Cat#E-CK-A105 BD GolgiPlugTM Protein Transport Inhibitor BD Pharmingen Cat#555029; RRID: AB_2869014 Leukocyte Activation Cocktail BD Pharmingen Cat#550583; RRID: AB_2868893 Dithiothreitol (DTT) Thermo Fisher Scientific Cat#A39255 Isopropyl b-D-1 thiogalactopyranoside (IPTG) Takara Cat#9030 Standard protein marker Thermo Fisher Scientific Cat#26616 Recombinant A35 protein This paper N/A Recombinant M1 protein This paper N/A Recombinant GvO-DAM protein This paper N/A Recombinant Sd-Ferritin protein This paper N/A Recombinant DAM protein This paper N/A Recombinant A29 protein This paper N/A Recombinant B6 protein This paper N/A Recombinant E8 protein This paper N/A Recombinant Ferritin protein This paper N/A Critical commercial assays Plasmid extraction kit Macherey-Nagel Cat#740412.50 ClonExpress Ultra One Step Cloning Kit Vazyme Cat#C117-02 BCA protein assay kit Thermo Fisher Scientific Cat#A55864 FastPure Viral DNA/RNA Mini Kit Pro Vazyme Cat#RC323-01 2xChamQ Universal SYBR qPCR Master Mix Vazyme Cat#Q711-02 Cytofix/CytopermTM Fixation/ Permeabilization Kit BD Bioscience Cat#554714; RRID: AB_2869008 ToxinSensor Chromogenic LAL Endotoxin Assay Kit GenScript Cat#L00350 Experimental models: Cell lines Expi 293F Thermo Fisher Scientific Cat#A14527 Vero E6 ATCC Cat#CRL1586 (Continued on next page) 18 Cell Reports 44, 116358, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Balb/C mice Guangdong Medical Laboratory Animal Center N/A New Zealand rabbit Guangdong Medical Laboratory Animal Center N/A Oligonucleotides VACV-F 5′-ACATCTGGAGAATCCACAACA-3′ Ren et al.51 N/A VACV-R 5′-CATCATCGGTGGTTGATTTA-3′ Ren et al.51 N/A VACV HA TaqMan probe 5′- FAM-GAGACTCCGGAACCAATTAMRA-3′ Ren et al.51 N/A Recombinant DNA VRC8400-M1R-his This paper N/A VRC8400-B6R-his This paper N/A VRC8400-GVO-DAM-his This paper N/A VRC8400-DAM-his This paper N/A pTO-T7-Sd-Ferritin This paper N/A pTO-T7-Ferritin This paper N/A pTO-T7-A35R-his This paper N/A pTO-T7-A29L-his This paper N/A pTO-T7-E8L-his This paper N/A pCAGGS-7D11(VH)-IgG1 This paper N/A pCAGGS-7D11(VL)-IgG1 This paper N/A pCAGGS-A27D7(VH)-IgG2a This paper N/A pCAGGS-A27D7(VL)-IgG2a This paper N/A Software and algorithms GraphPad Prism V9.5.1 Graphpad https://www.graphpad.com/ SlideViewer 3DHISTECH Ltd. https://www.3dhistech.com/ Tanon 1600 automatic Digital Gel Image Analysis system Tanon https://tanon.cnreagent.com/ Zetasizer Ultra ZS Xplorer V2.0 Malvern Panalytical https://www.malvernpanalytical.com/ PyMol The PyMOL Molecular Graphics System, Version 2.0 Schrö dinger, LLC. https://pymol.org/ FlowJo V10 FlowJo https://www.flowjo.com Octet BLI Analysis 12.2 Fortebio N/A Other Expi 293F serum-free medium OPM Biosciences Cat#AC601501-1000mL Protein A Resin GenScript Cat#L00210-50 Protein G Resin GenScript Cat#L00209-50 Filter paper Cytiva Cat#1004047 Superdex 200 Increase 10/300 GL column Cytiva Cat#28990944 Superose 6 Increase 10/300 GL column Cytiva Cat#29091596 HiLoad 26/600 Superdex 200 pg SEC column Cytiva Cat#28989336 Ni Sepharose excel resin Cytiva Cat#17371203 HiScreen Capto Core 700 column Cytiva Cat#17548115 Capto DEAE column Cytiva Cat#17544310 10K MWCO ultracentrifuge tube Merck millipore Cat#UFC901096 (Continued on next page) Cell Reports 44, 116358, October 28, 2025 19

    Marker:

    Article Title: A single-dose nanoparticle vaccine protects against mpox in preclinical models.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE-CyTM7 Hamster Anti-Mouse CD3e, clone 145-2C11 BD Pharmingen Cat#135210; RRID: AB_2159183 BV510 Rat Anti-Mouse CD4, clone RM4-5 BD Pharmingen Cat#563106; RRID:AB_2687550 PerCP-CyTM5.5 Rat Anti-Mouse CD8a, clone 53–6.7 BD Pharmingen Cat#551162; RRID:AB_394081 Brilliant Violet 421TM anti-mouse IL-4 Antibody, clone 11B11 BioLegend Cat#504120; RRID:AB_2562102 FITC Rat Anti-Mouse IFN-γ, clone XMG1.2 BD Pharmingen Cat#554411; RRID:AB_AB_395375 PE Rat Anti-Mouse TNF, clone MP6-XT22 BD Pharmingen Cat#554419; RRID:AB_395380 Purified Rat Anti-Mouse CD16/CD32, clone 2.4G2 BD Pharmingen Cat#553141; RRID:AB_394656 PerCP/Cyanine5.5 anti-mouse/human CD45R/B220, clone RA3-6B2 BioLegend Cat#103236; RRID:AB_893354 PE Rat anti-Mouse IgD, clone 11-26c.2a BD Pharmingen Cat#558597; RRID:AB_647211 Alexa Fluor® 647 Rat Anti-Mouse T- and B-Cell Activation Antigen, Clone GL7 BD Pharmingen Cat#561529; RRID:AB_10716056 FITC anti-mouse CD95 (Fas) Antibody, clone SA367H8 BioLegend Cat#152606; RRID:AB_2632901 PE anti-mouse CD185 (CXCR5) antibody, clone SPRCL5 eBioscience Cat#12-7185-82; RRID: AB_11217882 APC anti-mouse CD279 (PD-1) Antibody, Clone 29F.1A12 BioLegend Cat#135210; RRID:AB_2159183 Goat Anti-Mouse IgG H&L (HRP) Abcam Cat#ab6789; RRID:AB_955439 Goat Anti-Mouse IgG1 (HRP) Abcam Cat#ab97240; RRID: AB_10695944 Goat Anti-Mouse IgG2a (HRP) Abcam Cat#ab97245; RRID:AB_10680049 Anti-Rabbit IgG (H + L) (HRP) Invitrogen Cat#A16096; RRID:AB_2534770 Goat Anti-Human IgG - H&L (HRP) Abcam Cat#ab6858; RRID:AB_955433 7D11 This paper N/A A27D7 This paper N/A M1H11 Ren et al.51 N/A Bacterial and virus strains DH5α competent cells AlpaLifeBio Cat#KTSM101L Rosetta (DE3) competent cells Tiangen Cat#CB108-02 VACV Tiantan strain Zhang et al.75 N/A VTT-GFP strain Ren et al.51 N/A VACV Western Reserve strain Zuo et al.67 N/A Clade I mpox virus This paper N/A Clade II mpox virus(hMpxV/China/SZSZTH41/2023) Cheng et al.68 N/A Biological samples Serum samples from immunized BALB/c mice This paper N/A Serum samples from immunized New Zealand rabbits This paper N/A MPXV human convalescent sera This paper N/A (Continued on next page) Cell Reports 44, 116358, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins FH002C adjuvant Wu et al.52 N/A Sulfo-NHS-LC-LC-biotin Thermo Fisher Scientific Cat#21338 TMB substrate Tiangen CatPA107-02 BamHI-HF NEB Cat#R3136L NdeI NEB Cat#R0111L XhoI NEB Cat#R0146M Coomassie Brilliant Blue R250 Meilunbio Cat#MB4685 Bovine serum albumin V (BSA V) Biofroxx Cat#4240GR500 Tween 20 Biofroxx Cat#1247ML500 Trehalose Bioss Cat#D10226 TritonTM X-114 Sigma-Aldrich Cat#X114-100ML 2 × Phanta Max Master Mix Vazyme Cat#P515-03 PEI-MAX Polyscience Cat#24765-1 ELISA coated solution (1x) Solarbio Cat#C1050 ELISA Stop Solution Solarbio Cat#C1058 4% polyformaldehyde Yuanye Cat#R20497 Avicel FMC biopolymer Cat#RC-591 10×ACK lysis buffer Elabscience Cat#E-CK-A105 BD GolgiPlugTM Protein Transport Inhibitor BD Pharmingen Cat#555029; RRID: AB_2869014 Leukocyte Activation Cocktail BD Pharmingen Cat#550583; RRID: AB_2868893 Dithiothreitol (DTT) Thermo Fisher Scientific Cat#A39255 Isopropyl b-D-1 thiogalactopyranoside (IPTG) Takara Cat#9030 Standard protein marker Thermo Fisher Scientific Cat#26616 Recombinant A35 protein This paper N/A Recombinant M1 protein This paper N/A Recombinant GvO-DAM protein This paper N/A Recombinant Sd-Ferritin protein This paper N/A Recombinant DAM protein This paper N/A Recombinant A29 protein This paper N/A Recombinant B6 protein This paper N/A Recombinant E8 protein This paper N/A Recombinant Ferritin protein This paper N/A Critical commercial assays Plasmid extraction kit Macherey-Nagel Cat#740412.50 ClonExpress Ultra One Step Cloning Kit Vazyme Cat#C117-02 BCA protein assay kit Thermo Fisher Scientific Cat#A55864 FastPure Viral DNA/RNA Mini Kit Pro Vazyme Cat#RC323-01 2xChamQ Universal SYBR qPCR Master Mix Vazyme Cat#Q711-02 Cytofix/CytopermTM Fixation/ Permeabilization Kit BD Bioscience Cat#554714; RRID: AB_2869008 ToxinSensor Chromogenic LAL Endotoxin Assay Kit GenScript Cat#L00350 Experimental models: Cell lines Expi 293F Thermo Fisher Scientific Cat#A14527 Vero E6 ATCC Cat#CRL1586 (Continued on next page) 18 Cell Reports 44, 116358, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Balb/C mice Guangdong Medical Laboratory Animal Center N/A New Zealand rabbit Guangdong Medical Laboratory Animal Center N/A Oligonucleotides VACV-F 5′-ACATCTGGAGAATCCACAACA-3′ Ren et al.51 N/A VACV-R 5′-CATCATCGGTGGTTGATTTA-3′ Ren et al.51 N/A VACV HA TaqMan probe 5′- FAM-GAGACTCCGGAACCAATTAMRA-3′ Ren et al.51 N/A Recombinant DNA VRC8400-M1R-his This paper N/A VRC8400-B6R-his This paper N/A VRC8400-GVO-DAM-his This paper N/A VRC8400-DAM-his This paper N/A pTO-T7-Sd-Ferritin This paper N/A pTO-T7-Ferritin This paper N/A pTO-T7-A35R-his This paper N/A pTO-T7-A29L-his This paper N/A pTO-T7-E8L-his This paper N/A pCAGGS-7D11(VH)-IgG1 This paper N/A pCAGGS-7D11(VL)-IgG1 This paper N/A pCAGGS-A27D7(VH)-IgG2a This paper N/A pCAGGS-A27D7(VL)-IgG2a This paper N/A Software and algorithms GraphPad Prism V9.5.1 Graphpad https://www.graphpad.com/ SlideViewer 3DHISTECH Ltd. https://www.3dhistech.com/ Tanon 1600 automatic Digital Gel Image Analysis system Tanon https://tanon.cnreagent.com/ Zetasizer Ultra ZS Xplorer V2.0 Malvern Panalytical https://www.malvernpanalytical.com/ PyMol The PyMOL Molecular Graphics System, Version 2.0 Schrö dinger, LLC. https://pymol.org/ FlowJo V10 FlowJo https://www.flowjo.com Octet BLI Analysis 12.2 Fortebio N/A Other Expi 293F serum-free medium OPM Biosciences Cat#AC601501-1000mL Protein A Resin GenScript Cat#L00210-50 Protein G Resin GenScript Cat#L00209-50 Filter paper Cytiva Cat#1004047 Superdex 200 Increase 10/300 GL column Cytiva Cat#28990944 Superose 6 Increase 10/300 GL column Cytiva Cat#29091596 HiLoad 26/600 Superdex 200 pg SEC column Cytiva Cat#28989336 Ni Sepharose excel resin Cytiva Cat#17371203 HiScreen Capto Core 700 column Cytiva Cat#17548115 Capto DEAE column Cytiva Cat#17544310 10K MWCO ultracentrifuge tube Merck millipore Cat#UFC901096 (Continued on next page) Cell Reports 44, 116358, October 28, 2025 19

    Plasmid Preparation:

    Article Title: A single-dose nanoparticle vaccine protects against mpox in preclinical models.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE-CyTM7 Hamster Anti-Mouse CD3e, clone 145-2C11 BD Pharmingen Cat#135210; RRID: AB_2159183 BV510 Rat Anti-Mouse CD4, clone RM4-5 BD Pharmingen Cat#563106; RRID:AB_2687550 PerCP-CyTM5.5 Rat Anti-Mouse CD8a, clone 53–6.7 BD Pharmingen Cat#551162; RRID:AB_394081 Brilliant Violet 421TM anti-mouse IL-4 Antibody, clone 11B11 BioLegend Cat#504120; RRID:AB_2562102 FITC Rat Anti-Mouse IFN-γ, clone XMG1.2 BD Pharmingen Cat#554411; RRID:AB_AB_395375 PE Rat Anti-Mouse TNF, clone MP6-XT22 BD Pharmingen Cat#554419; RRID:AB_395380 Purified Rat Anti-Mouse CD16/CD32, clone 2.4G2 BD Pharmingen Cat#553141; RRID:AB_394656 PerCP/Cyanine5.5 anti-mouse/human CD45R/B220, clone RA3-6B2 BioLegend Cat#103236; RRID:AB_893354 PE Rat anti-Mouse IgD, clone 11-26c.2a BD Pharmingen Cat#558597; RRID:AB_647211 Alexa Fluor® 647 Rat Anti-Mouse T- and B-Cell Activation Antigen, Clone GL7 BD Pharmingen Cat#561529; RRID:AB_10716056 FITC anti-mouse CD95 (Fas) Antibody, clone SA367H8 BioLegend Cat#152606; RRID:AB_2632901 PE anti-mouse CD185 (CXCR5) antibody, clone SPRCL5 eBioscience Cat#12-7185-82; RRID: AB_11217882 APC anti-mouse CD279 (PD-1) Antibody, Clone 29F.1A12 BioLegend Cat#135210; RRID:AB_2159183 Goat Anti-Mouse IgG H&L (HRP) Abcam Cat#ab6789; RRID:AB_955439 Goat Anti-Mouse IgG1 (HRP) Abcam Cat#ab97240; RRID: AB_10695944 Goat Anti-Mouse IgG2a (HRP) Abcam Cat#ab97245; RRID:AB_10680049 Anti-Rabbit IgG (H + L) (HRP) Invitrogen Cat#A16096; RRID:AB_2534770 Goat Anti-Human IgG - H&L (HRP) Abcam Cat#ab6858; RRID:AB_955433 7D11 This paper N/A A27D7 This paper N/A M1H11 Ren et al.51 N/A Bacterial and virus strains DH5α competent cells AlpaLifeBio Cat#KTSM101L Rosetta (DE3) competent cells Tiangen Cat#CB108-02 VACV Tiantan strain Zhang et al.75 N/A VTT-GFP strain Ren et al.51 N/A VACV Western Reserve strain Zuo et al.67 N/A Clade I mpox virus This paper N/A Clade II mpox virus(hMpxV/China/SZSZTH41/2023) Cheng et al.68 N/A Biological samples Serum samples from immunized BALB/c mice This paper N/A Serum samples from immunized New Zealand rabbits This paper N/A MPXV human convalescent sera This paper N/A (Continued on next page) Cell Reports 44, 116358, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins FH002C adjuvant Wu et al.52 N/A Sulfo-NHS-LC-LC-biotin Thermo Fisher Scientific Cat#21338 TMB substrate Tiangen CatPA107-02 BamHI-HF NEB Cat#R3136L NdeI NEB Cat#R0111L XhoI NEB Cat#R0146M Coomassie Brilliant Blue R250 Meilunbio Cat#MB4685 Bovine serum albumin V (BSA V) Biofroxx Cat#4240GR500 Tween 20 Biofroxx Cat#1247ML500 Trehalose Bioss Cat#D10226 TritonTM X-114 Sigma-Aldrich Cat#X114-100ML 2 × Phanta Max Master Mix Vazyme Cat#P515-03 PEI-MAX Polyscience Cat#24765-1 ELISA coated solution (1x) Solarbio Cat#C1050 ELISA Stop Solution Solarbio Cat#C1058 4% polyformaldehyde Yuanye Cat#R20497 Avicel FMC biopolymer Cat#RC-591 10×ACK lysis buffer Elabscience Cat#E-CK-A105 BD GolgiPlugTM Protein Transport Inhibitor BD Pharmingen Cat#555029; RRID: AB_2869014 Leukocyte Activation Cocktail BD Pharmingen Cat#550583; RRID: AB_2868893 Dithiothreitol (DTT) Thermo Fisher Scientific Cat#A39255 Isopropyl b-D-1 thiogalactopyranoside (IPTG) Takara Cat#9030 Standard protein marker Thermo Fisher Scientific Cat#26616 Recombinant A35 protein This paper N/A Recombinant M1 protein This paper N/A Recombinant GvO-DAM protein This paper N/A Recombinant Sd-Ferritin protein This paper N/A Recombinant DAM protein This paper N/A Recombinant A29 protein This paper N/A Recombinant B6 protein This paper N/A Recombinant E8 protein This paper N/A Recombinant Ferritin protein This paper N/A Critical commercial assays Plasmid extraction kit Macherey-Nagel Cat#740412.50 ClonExpress Ultra One Step Cloning Kit Vazyme Cat#C117-02 BCA protein assay kit Thermo Fisher Scientific Cat#A55864 FastPure Viral DNA/RNA Mini Kit Pro Vazyme Cat#RC323-01 2xChamQ Universal SYBR qPCR Master Mix Vazyme Cat#Q711-02 Cytofix/CytopermTM Fixation/ Permeabilization Kit BD Bioscience Cat#554714; RRID: AB_2869008 ToxinSensor Chromogenic LAL Endotoxin Assay Kit GenScript Cat#L00350 Experimental models: Cell lines Expi 293F Thermo Fisher Scientific Cat#A14527 Vero E6 ATCC Cat#CRL1586 (Continued on next page) 18 Cell Reports 44, 116358, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Balb/C mice Guangdong Medical Laboratory Animal Center N/A New Zealand rabbit Guangdong Medical Laboratory Animal Center N/A Oligonucleotides VACV-F 5′-ACATCTGGAGAATCCACAACA-3′ Ren et al.51 N/A VACV-R 5′-CATCATCGGTGGTTGATTTA-3′ Ren et al.51 N/A VACV HA TaqMan probe 5′- FAM-GAGACTCCGGAACCAATTAMRA-3′ Ren et al.51 N/A Recombinant DNA VRC8400-M1R-his This paper N/A VRC8400-B6R-his This paper N/A VRC8400-GVO-DAM-his This paper N/A VRC8400-DAM-his This paper N/A pTO-T7-Sd-Ferritin This paper N/A pTO-T7-Ferritin This paper N/A pTO-T7-A35R-his This paper N/A pTO-T7-A29L-his This paper N/A pTO-T7-E8L-his This paper N/A pCAGGS-7D11(VH)-IgG1 This paper N/A pCAGGS-7D11(VL)-IgG1 This paper N/A pCAGGS-A27D7(VH)-IgG2a This paper N/A pCAGGS-A27D7(VL)-IgG2a This paper N/A Software and algorithms GraphPad Prism V9.5.1 Graphpad https://www.graphpad.com/ SlideViewer 3DHISTECH Ltd. https://www.3dhistech.com/ Tanon 1600 automatic Digital Gel Image Analysis system Tanon https://tanon.cnreagent.com/ Zetasizer Ultra ZS Xplorer V2.0 Malvern Panalytical https://www.malvernpanalytical.com/ PyMol The PyMOL Molecular Graphics System, Version 2.0 Schrö dinger, LLC. https://pymol.org/ FlowJo V10 FlowJo https://www.flowjo.com Octet BLI Analysis 12.2 Fortebio N/A Other Expi 293F serum-free medium OPM Biosciences Cat#AC601501-1000mL Protein A Resin GenScript Cat#L00210-50 Protein G Resin GenScript Cat#L00209-50 Filter paper Cytiva Cat#1004047 Superdex 200 Increase 10/300 GL column Cytiva Cat#28990944 Superose 6 Increase 10/300 GL column Cytiva Cat#29091596 HiLoad 26/600 Superdex 200 pg SEC column Cytiva Cat#28989336 Ni Sepharose excel resin Cytiva Cat#17371203 HiScreen Capto Core 700 column Cytiva Cat#17548115 Capto DEAE column Cytiva Cat#17544310 10K MWCO ultracentrifuge tube Merck millipore Cat#UFC901096 (Continued on next page) Cell Reports 44, 116358, October 28, 2025 19

    Extraction:

    Article Title: A single-dose nanoparticle vaccine protects against mpox in preclinical models.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE-CyTM7 Hamster Anti-Mouse CD3e, clone 145-2C11 BD Pharmingen Cat#135210; RRID: AB_2159183 BV510 Rat Anti-Mouse CD4, clone RM4-5 BD Pharmingen Cat#563106; RRID:AB_2687550 PerCP-CyTM5.5 Rat Anti-Mouse CD8a, clone 53–6.7 BD Pharmingen Cat#551162; RRID:AB_394081 Brilliant Violet 421TM anti-mouse IL-4 Antibody, clone 11B11 BioLegend Cat#504120; RRID:AB_2562102 FITC Rat Anti-Mouse IFN-γ, clone XMG1.2 BD Pharmingen Cat#554411; RRID:AB_AB_395375 PE Rat Anti-Mouse TNF, clone MP6-XT22 BD Pharmingen Cat#554419; RRID:AB_395380 Purified Rat Anti-Mouse CD16/CD32, clone 2.4G2 BD Pharmingen Cat#553141; RRID:AB_394656 PerCP/Cyanine5.5 anti-mouse/human CD45R/B220, clone RA3-6B2 BioLegend Cat#103236; RRID:AB_893354 PE Rat anti-Mouse IgD, clone 11-26c.2a BD Pharmingen Cat#558597; RRID:AB_647211 Alexa Fluor® 647 Rat Anti-Mouse T- and B-Cell Activation Antigen, Clone GL7 BD Pharmingen Cat#561529; RRID:AB_10716056 FITC anti-mouse CD95 (Fas) Antibody, clone SA367H8 BioLegend Cat#152606; RRID:AB_2632901 PE anti-mouse CD185 (CXCR5) antibody, clone SPRCL5 eBioscience Cat#12-7185-82; RRID: AB_11217882 APC anti-mouse CD279 (PD-1) Antibody, Clone 29F.1A12 BioLegend Cat#135210; RRID:AB_2159183 Goat Anti-Mouse IgG H&L (HRP) Abcam Cat#ab6789; RRID:AB_955439 Goat Anti-Mouse IgG1 (HRP) Abcam Cat#ab97240; RRID: AB_10695944 Goat Anti-Mouse IgG2a (HRP) Abcam Cat#ab97245; RRID:AB_10680049 Anti-Rabbit IgG (H + L) (HRP) Invitrogen Cat#A16096; RRID:AB_2534770 Goat Anti-Human IgG - H&L (HRP) Abcam Cat#ab6858; RRID:AB_955433 7D11 This paper N/A A27D7 This paper N/A M1H11 Ren et al.51 N/A Bacterial and virus strains DH5α competent cells AlpaLifeBio Cat#KTSM101L Rosetta (DE3) competent cells Tiangen Cat#CB108-02 VACV Tiantan strain Zhang et al.75 N/A VTT-GFP strain Ren et al.51 N/A VACV Western Reserve strain Zuo et al.67 N/A Clade I mpox virus This paper N/A Clade II mpox virus(hMpxV/China/SZSZTH41/2023) Cheng et al.68 N/A Biological samples Serum samples from immunized BALB/c mice This paper N/A Serum samples from immunized New Zealand rabbits This paper N/A MPXV human convalescent sera This paper N/A (Continued on next page) Cell Reports 44, 116358, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins FH002C adjuvant Wu et al.52 N/A Sulfo-NHS-LC-LC-biotin Thermo Fisher Scientific Cat#21338 TMB substrate Tiangen CatPA107-02 BamHI-HF NEB Cat#R3136L NdeI NEB Cat#R0111L XhoI NEB Cat#R0146M Coomassie Brilliant Blue R250 Meilunbio Cat#MB4685 Bovine serum albumin V (BSA V) Biofroxx Cat#4240GR500 Tween 20 Biofroxx Cat#1247ML500 Trehalose Bioss Cat#D10226 TritonTM X-114 Sigma-Aldrich Cat#X114-100ML 2 × Phanta Max Master Mix Vazyme Cat#P515-03 PEI-MAX Polyscience Cat#24765-1 ELISA coated solution (1x) Solarbio Cat#C1050 ELISA Stop Solution Solarbio Cat#C1058 4% polyformaldehyde Yuanye Cat#R20497 Avicel FMC biopolymer Cat#RC-591 10×ACK lysis buffer Elabscience Cat#E-CK-A105 BD GolgiPlugTM Protein Transport Inhibitor BD Pharmingen Cat#555029; RRID: AB_2869014 Leukocyte Activation Cocktail BD Pharmingen Cat#550583; RRID: AB_2868893 Dithiothreitol (DTT) Thermo Fisher Scientific Cat#A39255 Isopropyl b-D-1 thiogalactopyranoside (IPTG) Takara Cat#9030 Standard protein marker Thermo Fisher Scientific Cat#26616 Recombinant A35 protein This paper N/A Recombinant M1 protein This paper N/A Recombinant GvO-DAM protein This paper N/A Recombinant Sd-Ferritin protein This paper N/A Recombinant DAM protein This paper N/A Recombinant A29 protein This paper N/A Recombinant B6 protein This paper N/A Recombinant E8 protein This paper N/A Recombinant Ferritin protein This paper N/A Critical commercial assays Plasmid extraction kit Macherey-Nagel Cat#740412.50 ClonExpress Ultra One Step Cloning Kit Vazyme Cat#C117-02 BCA protein assay kit Thermo Fisher Scientific Cat#A55864 FastPure Viral DNA/RNA Mini Kit Pro Vazyme Cat#RC323-01 2xChamQ Universal SYBR qPCR Master Mix Vazyme Cat#Q711-02 Cytofix/CytopermTM Fixation/ Permeabilization Kit BD Bioscience Cat#554714; RRID: AB_2869008 ToxinSensor Chromogenic LAL Endotoxin Assay Kit GenScript Cat#L00350 Experimental models: Cell lines Expi 293F Thermo Fisher Scientific Cat#A14527 Vero E6 ATCC Cat#CRL1586 (Continued on next page) 18 Cell Reports 44, 116358, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Balb/C mice Guangdong Medical Laboratory Animal Center N/A New Zealand rabbit Guangdong Medical Laboratory Animal Center N/A Oligonucleotides VACV-F 5′-ACATCTGGAGAATCCACAACA-3′ Ren et al.51 N/A VACV-R 5′-CATCATCGGTGGTTGATTTA-3′ Ren et al.51 N/A VACV HA TaqMan probe 5′- FAM-GAGACTCCGGAACCAATTAMRA-3′ Ren et al.51 N/A Recombinant DNA VRC8400-M1R-his This paper N/A VRC8400-B6R-his This paper N/A VRC8400-GVO-DAM-his This paper N/A VRC8400-DAM-his This paper N/A pTO-T7-Sd-Ferritin This paper N/A pTO-T7-Ferritin This paper N/A pTO-T7-A35R-his This paper N/A pTO-T7-A29L-his This paper N/A pTO-T7-E8L-his This paper N/A pCAGGS-7D11(VH)-IgG1 This paper N/A pCAGGS-7D11(VL)-IgG1 This paper N/A pCAGGS-A27D7(VH)-IgG2a This paper N/A pCAGGS-A27D7(VL)-IgG2a This paper N/A Software and algorithms GraphPad Prism V9.5.1 Graphpad https://www.graphpad.com/ SlideViewer 3DHISTECH Ltd. https://www.3dhistech.com/ Tanon 1600 automatic Digital Gel Image Analysis system Tanon https://tanon.cnreagent.com/ Zetasizer Ultra ZS Xplorer V2.0 Malvern Panalytical https://www.malvernpanalytical.com/ PyMol The PyMOL Molecular Graphics System, Version 2.0 Schrö dinger, LLC. https://pymol.org/ FlowJo V10 FlowJo https://www.flowjo.com Octet BLI Analysis 12.2 Fortebio N/A Other Expi 293F serum-free medium OPM Biosciences Cat#AC601501-1000mL Protein A Resin GenScript Cat#L00210-50 Protein G Resin GenScript Cat#L00209-50 Filter paper Cytiva Cat#1004047 Superdex 200 Increase 10/300 GL column Cytiva Cat#28990944 Superose 6 Increase 10/300 GL column Cytiva Cat#29091596 HiLoad 26/600 Superdex 200 pg SEC column Cytiva Cat#28989336 Ni Sepharose excel resin Cytiva Cat#17371203 HiScreen Capto Core 700 column Cytiva Cat#17548115 Capto DEAE column Cytiva Cat#17544310 10K MWCO ultracentrifuge tube Merck millipore Cat#UFC901096 (Continued on next page) Cell Reports 44, 116358, October 28, 2025 19

    Cloning:

    Article Title: A single-dose nanoparticle vaccine protects against mpox in preclinical models.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE-CyTM7 Hamster Anti-Mouse CD3e, clone 145-2C11 BD Pharmingen Cat#135210; RRID: AB_2159183 BV510 Rat Anti-Mouse CD4, clone RM4-5 BD Pharmingen Cat#563106; RRID:AB_2687550 PerCP-CyTM5.5 Rat Anti-Mouse CD8a, clone 53–6.7 BD Pharmingen Cat#551162; RRID:AB_394081 Brilliant Violet 421TM anti-mouse IL-4 Antibody, clone 11B11 BioLegend Cat#504120; RRID:AB_2562102 FITC Rat Anti-Mouse IFN-γ, clone XMG1.2 BD Pharmingen Cat#554411; RRID:AB_AB_395375 PE Rat Anti-Mouse TNF, clone MP6-XT22 BD Pharmingen Cat#554419; RRID:AB_395380 Purified Rat Anti-Mouse CD16/CD32, clone 2.4G2 BD Pharmingen Cat#553141; RRID:AB_394656 PerCP/Cyanine5.5 anti-mouse/human CD45R/B220, clone RA3-6B2 BioLegend Cat#103236; RRID:AB_893354 PE Rat anti-Mouse IgD, clone 11-26c.2a BD Pharmingen Cat#558597; RRID:AB_647211 Alexa Fluor® 647 Rat Anti-Mouse T- and B-Cell Activation Antigen, Clone GL7 BD Pharmingen Cat#561529; RRID:AB_10716056 FITC anti-mouse CD95 (Fas) Antibody, clone SA367H8 BioLegend Cat#152606; RRID:AB_2632901 PE anti-mouse CD185 (CXCR5) antibody, clone SPRCL5 eBioscience Cat#12-7185-82; RRID: AB_11217882 APC anti-mouse CD279 (PD-1) Antibody, Clone 29F.1A12 BioLegend Cat#135210; RRID:AB_2159183 Goat Anti-Mouse IgG H&L (HRP) Abcam Cat#ab6789; RRID:AB_955439 Goat Anti-Mouse IgG1 (HRP) Abcam Cat#ab97240; RRID: AB_10695944 Goat Anti-Mouse IgG2a (HRP) Abcam Cat#ab97245; RRID:AB_10680049 Anti-Rabbit IgG (H + L) (HRP) Invitrogen Cat#A16096; RRID:AB_2534770 Goat Anti-Human IgG - H&L (HRP) Abcam Cat#ab6858; RRID:AB_955433 7D11 This paper N/A A27D7 This paper N/A M1H11 Ren et al.51 N/A Bacterial and virus strains DH5α competent cells AlpaLifeBio Cat#KTSM101L Rosetta (DE3) competent cells Tiangen Cat#CB108-02 VACV Tiantan strain Zhang et al.75 N/A VTT-GFP strain Ren et al.51 N/A VACV Western Reserve strain Zuo et al.67 N/A Clade I mpox virus This paper N/A Clade II mpox virus(hMpxV/China/SZSZTH41/2023) Cheng et al.68 N/A Biological samples Serum samples from immunized BALB/c mice This paper N/A Serum samples from immunized New Zealand rabbits This paper N/A MPXV human convalescent sera This paper N/A (Continued on next page) Cell Reports 44, 116358, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins FH002C adjuvant Wu et al.52 N/A Sulfo-NHS-LC-LC-biotin Thermo Fisher Scientific Cat#21338 TMB substrate Tiangen CatPA107-02 BamHI-HF NEB Cat#R3136L NdeI NEB Cat#R0111L XhoI NEB Cat#R0146M Coomassie Brilliant Blue R250 Meilunbio Cat#MB4685 Bovine serum albumin V (BSA V) Biofroxx Cat#4240GR500 Tween 20 Biofroxx Cat#1247ML500 Trehalose Bioss Cat#D10226 TritonTM X-114 Sigma-Aldrich Cat#X114-100ML 2 × Phanta Max Master Mix Vazyme Cat#P515-03 PEI-MAX Polyscience Cat#24765-1 ELISA coated solution (1x) Solarbio Cat#C1050 ELISA Stop Solution Solarbio Cat#C1058 4% polyformaldehyde Yuanye Cat#R20497 Avicel FMC biopolymer Cat#RC-591 10×ACK lysis buffer Elabscience Cat#E-CK-A105 BD GolgiPlugTM Protein Transport Inhibitor BD Pharmingen Cat#555029; RRID: AB_2869014 Leukocyte Activation Cocktail BD Pharmingen Cat#550583; RRID: AB_2868893 Dithiothreitol (DTT) Thermo Fisher Scientific Cat#A39255 Isopropyl b-D-1 thiogalactopyranoside (IPTG) Takara Cat#9030 Standard protein marker Thermo Fisher Scientific Cat#26616 Recombinant A35 protein This paper N/A Recombinant M1 protein This paper N/A Recombinant GvO-DAM protein This paper N/A Recombinant Sd-Ferritin protein This paper N/A Recombinant DAM protein This paper N/A Recombinant A29 protein This paper N/A Recombinant B6 protein This paper N/A Recombinant E8 protein This paper N/A Recombinant Ferritin protein This paper N/A Critical commercial assays Plasmid extraction kit Macherey-Nagel Cat#740412.50 ClonExpress Ultra One Step Cloning Kit Vazyme Cat#C117-02 BCA protein assay kit Thermo Fisher Scientific Cat#A55864 FastPure Viral DNA/RNA Mini Kit Pro Vazyme Cat#RC323-01 2xChamQ Universal SYBR qPCR Master Mix Vazyme Cat#Q711-02 Cytofix/CytopermTM Fixation/ Permeabilization Kit BD Bioscience Cat#554714; RRID: AB_2869008 ToxinSensor Chromogenic LAL Endotoxin Assay Kit GenScript Cat#L00350 Experimental models: Cell lines Expi 293F Thermo Fisher Scientific Cat#A14527 Vero E6 ATCC Cat#CRL1586 (Continued on next page) 18 Cell Reports 44, 116358, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Balb/C mice Guangdong Medical Laboratory Animal Center N/A New Zealand rabbit Guangdong Medical Laboratory Animal Center N/A Oligonucleotides VACV-F 5′-ACATCTGGAGAATCCACAACA-3′ Ren et al.51 N/A VACV-R 5′-CATCATCGGTGGTTGATTTA-3′ Ren et al.51 N/A VACV HA TaqMan probe 5′- FAM-GAGACTCCGGAACCAATTAMRA-3′ Ren et al.51 N/A Recombinant DNA VRC8400-M1R-his This paper N/A VRC8400-B6R-his This paper N/A VRC8400-GVO-DAM-his This paper N/A VRC8400-DAM-his This paper N/A pTO-T7-Sd-Ferritin This paper N/A pTO-T7-Ferritin This paper N/A pTO-T7-A35R-his This paper N/A pTO-T7-A29L-his This paper N/A pTO-T7-E8L-his This paper N/A pCAGGS-7D11(VH)-IgG1 This paper N/A pCAGGS-7D11(VL)-IgG1 This paper N/A pCAGGS-A27D7(VH)-IgG2a This paper N/A pCAGGS-A27D7(VL)-IgG2a This paper N/A Software and algorithms GraphPad Prism V9.5.1 Graphpad https://www.graphpad.com/ SlideViewer 3DHISTECH Ltd. https://www.3dhistech.com/ Tanon 1600 automatic Digital Gel Image Analysis system Tanon https://tanon.cnreagent.com/ Zetasizer Ultra ZS Xplorer V2.0 Malvern Panalytical https://www.malvernpanalytical.com/ PyMol The PyMOL Molecular Graphics System, Version 2.0 Schrö dinger, LLC. https://pymol.org/ FlowJo V10 FlowJo https://www.flowjo.com Octet BLI Analysis 12.2 Fortebio N/A Other Expi 293F serum-free medium OPM Biosciences Cat#AC601501-1000mL Protein A Resin GenScript Cat#L00210-50 Protein G Resin GenScript Cat#L00209-50 Filter paper Cytiva Cat#1004047 Superdex 200 Increase 10/300 GL column Cytiva Cat#28990944 Superose 6 Increase 10/300 GL column Cytiva Cat#29091596 HiLoad 26/600 Superdex 200 pg SEC column Cytiva Cat#28989336 Ni Sepharose excel resin Cytiva Cat#17371203 HiScreen Capto Core 700 column Cytiva Cat#17548115 Capto DEAE column Cytiva Cat#17544310 10K MWCO ultracentrifuge tube Merck millipore Cat#UFC901096 (Continued on next page) Cell Reports 44, 116358, October 28, 2025 19

    Bicinchoninic Acid Protein Assay:

    Article Title: A single-dose nanoparticle vaccine protects against mpox in preclinical models.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE-CyTM7 Hamster Anti-Mouse CD3e, clone 145-2C11 BD Pharmingen Cat#135210; RRID: AB_2159183 BV510 Rat Anti-Mouse CD4, clone RM4-5 BD Pharmingen Cat#563106; RRID:AB_2687550 PerCP-CyTM5.5 Rat Anti-Mouse CD8a, clone 53–6.7 BD Pharmingen Cat#551162; RRID:AB_394081 Brilliant Violet 421TM anti-mouse IL-4 Antibody, clone 11B11 BioLegend Cat#504120; RRID:AB_2562102 FITC Rat Anti-Mouse IFN-γ, clone XMG1.2 BD Pharmingen Cat#554411; RRID:AB_AB_395375 PE Rat Anti-Mouse TNF, clone MP6-XT22 BD Pharmingen Cat#554419; RRID:AB_395380 Purified Rat Anti-Mouse CD16/CD32, clone 2.4G2 BD Pharmingen Cat#553141; RRID:AB_394656 PerCP/Cyanine5.5 anti-mouse/human CD45R/B220, clone RA3-6B2 BioLegend Cat#103236; RRID:AB_893354 PE Rat anti-Mouse IgD, clone 11-26c.2a BD Pharmingen Cat#558597; RRID:AB_647211 Alexa Fluor® 647 Rat Anti-Mouse T- and B-Cell Activation Antigen, Clone GL7 BD Pharmingen Cat#561529; RRID:AB_10716056 FITC anti-mouse CD95 (Fas) Antibody, clone SA367H8 BioLegend Cat#152606; RRID:AB_2632901 PE anti-mouse CD185 (CXCR5) antibody, clone SPRCL5 eBioscience Cat#12-7185-82; RRID: AB_11217882 APC anti-mouse CD279 (PD-1) Antibody, Clone 29F.1A12 BioLegend Cat#135210; RRID:AB_2159183 Goat Anti-Mouse IgG H&L (HRP) Abcam Cat#ab6789; RRID:AB_955439 Goat Anti-Mouse IgG1 (HRP) Abcam Cat#ab97240; RRID: AB_10695944 Goat Anti-Mouse IgG2a (HRP) Abcam Cat#ab97245; RRID:AB_10680049 Anti-Rabbit IgG (H + L) (HRP) Invitrogen Cat#A16096; RRID:AB_2534770 Goat Anti-Human IgG - H&L (HRP) Abcam Cat#ab6858; RRID:AB_955433 7D11 This paper N/A A27D7 This paper N/A M1H11 Ren et al.51 N/A Bacterial and virus strains DH5α competent cells AlpaLifeBio Cat#KTSM101L Rosetta (DE3) competent cells Tiangen Cat#CB108-02 VACV Tiantan strain Zhang et al.75 N/A VTT-GFP strain Ren et al.51 N/A VACV Western Reserve strain Zuo et al.67 N/A Clade I mpox virus This paper N/A Clade II mpox virus(hMpxV/China/SZSZTH41/2023) Cheng et al.68 N/A Biological samples Serum samples from immunized BALB/c mice This paper N/A Serum samples from immunized New Zealand rabbits This paper N/A MPXV human convalescent sera This paper N/A (Continued on next page) Cell Reports 44, 116358, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins FH002C adjuvant Wu et al.52 N/A Sulfo-NHS-LC-LC-biotin Thermo Fisher Scientific Cat#21338 TMB substrate Tiangen CatPA107-02 BamHI-HF NEB Cat#R3136L NdeI NEB Cat#R0111L XhoI NEB Cat#R0146M Coomassie Brilliant Blue R250 Meilunbio Cat#MB4685 Bovine serum albumin V (BSA V) Biofroxx Cat#4240GR500 Tween 20 Biofroxx Cat#1247ML500 Trehalose Bioss Cat#D10226 TritonTM X-114 Sigma-Aldrich Cat#X114-100ML 2 × Phanta Max Master Mix Vazyme Cat#P515-03 PEI-MAX Polyscience Cat#24765-1 ELISA coated solution (1x) Solarbio Cat#C1050 ELISA Stop Solution Solarbio Cat#C1058 4% polyformaldehyde Yuanye Cat#R20497 Avicel FMC biopolymer Cat#RC-591 10×ACK lysis buffer Elabscience Cat#E-CK-A105 BD GolgiPlugTM Protein Transport Inhibitor BD Pharmingen Cat#555029; RRID: AB_2869014 Leukocyte Activation Cocktail BD Pharmingen Cat#550583; RRID: AB_2868893 Dithiothreitol (DTT) Thermo Fisher Scientific Cat#A39255 Isopropyl b-D-1 thiogalactopyranoside (IPTG) Takara Cat#9030 Standard protein marker Thermo Fisher Scientific Cat#26616 Recombinant A35 protein This paper N/A Recombinant M1 protein This paper N/A Recombinant GvO-DAM protein This paper N/A Recombinant Sd-Ferritin protein This paper N/A Recombinant DAM protein This paper N/A Recombinant A29 protein This paper N/A Recombinant B6 protein This paper N/A Recombinant E8 protein This paper N/A Recombinant Ferritin protein This paper N/A Critical commercial assays Plasmid extraction kit Macherey-Nagel Cat#740412.50 ClonExpress Ultra One Step Cloning Kit Vazyme Cat#C117-02 BCA protein assay kit Thermo Fisher Scientific Cat#A55864 FastPure Viral DNA/RNA Mini Kit Pro Vazyme Cat#RC323-01 2xChamQ Universal SYBR qPCR Master Mix Vazyme Cat#Q711-02 Cytofix/CytopermTM Fixation/ Permeabilization Kit BD Bioscience Cat#554714; RRID: AB_2869008 ToxinSensor Chromogenic LAL Endotoxin Assay Kit GenScript Cat#L00350 Experimental models: Cell lines Expi 293F Thermo Fisher Scientific Cat#A14527 Vero E6 ATCC Cat#CRL1586 (Continued on next page) 18 Cell Reports 44, 116358, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Balb/C mice Guangdong Medical Laboratory Animal Center N/A New Zealand rabbit Guangdong Medical Laboratory Animal Center N/A Oligonucleotides VACV-F 5′-ACATCTGGAGAATCCACAACA-3′ Ren et al.51 N/A VACV-R 5′-CATCATCGGTGGTTGATTTA-3′ Ren et al.51 N/A VACV HA TaqMan probe 5′- FAM-GAGACTCCGGAACCAATTAMRA-3′ Ren et al.51 N/A Recombinant DNA VRC8400-M1R-his This paper N/A VRC8400-B6R-his This paper N/A VRC8400-GVO-DAM-his This paper N/A VRC8400-DAM-his This paper N/A pTO-T7-Sd-Ferritin This paper N/A pTO-T7-Ferritin This paper N/A pTO-T7-A35R-his This paper N/A pTO-T7-A29L-his This paper N/A pTO-T7-E8L-his This paper N/A pCAGGS-7D11(VH)-IgG1 This paper N/A pCAGGS-7D11(VL)-IgG1 This paper N/A pCAGGS-A27D7(VH)-IgG2a This paper N/A pCAGGS-A27D7(VL)-IgG2a This paper N/A Software and algorithms GraphPad Prism V9.5.1 Graphpad https://www.graphpad.com/ SlideViewer 3DHISTECH Ltd. https://www.3dhistech.com/ Tanon 1600 automatic Digital Gel Image Analysis system Tanon https://tanon.cnreagent.com/ Zetasizer Ultra ZS Xplorer V2.0 Malvern Panalytical https://www.malvernpanalytical.com/ PyMol The PyMOL Molecular Graphics System, Version 2.0 Schrö dinger, LLC. https://pymol.org/ FlowJo V10 FlowJo https://www.flowjo.com Octet BLI Analysis 12.2 Fortebio N/A Other Expi 293F serum-free medium OPM Biosciences Cat#AC601501-1000mL Protein A Resin GenScript Cat#L00210-50 Protein G Resin GenScript Cat#L00209-50 Filter paper Cytiva Cat#1004047 Superdex 200 Increase 10/300 GL column Cytiva Cat#28990944 Superose 6 Increase 10/300 GL column Cytiva Cat#29091596 HiLoad 26/600 Superdex 200 pg SEC column Cytiva Cat#28989336 Ni Sepharose excel resin Cytiva Cat#17371203 HiScreen Capto Core 700 column Cytiva Cat#17548115 Capto DEAE column Cytiva Cat#17544310 10K MWCO ultracentrifuge tube Merck millipore Cat#UFC901096 (Continued on next page) Cell Reports 44, 116358, October 28, 2025 19

    Real-time Polymerase Chain Reaction:

    Article Title: A single-dose nanoparticle vaccine protects against mpox in preclinical models.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE-CyTM7 Hamster Anti-Mouse CD3e, clone 145-2C11 BD Pharmingen Cat#135210; RRID: AB_2159183 BV510 Rat Anti-Mouse CD4, clone RM4-5 BD Pharmingen Cat#563106; RRID:AB_2687550 PerCP-CyTM5.5 Rat Anti-Mouse CD8a, clone 53–6.7 BD Pharmingen Cat#551162; RRID:AB_394081 Brilliant Violet 421TM anti-mouse IL-4 Antibody, clone 11B11 BioLegend Cat#504120; RRID:AB_2562102 FITC Rat Anti-Mouse IFN-γ, clone XMG1.2 BD Pharmingen Cat#554411; RRID:AB_AB_395375 PE Rat Anti-Mouse TNF, clone MP6-XT22 BD Pharmingen Cat#554419; RRID:AB_395380 Purified Rat Anti-Mouse CD16/CD32, clone 2.4G2 BD Pharmingen Cat#553141; RRID:AB_394656 PerCP/Cyanine5.5 anti-mouse/human CD45R/B220, clone RA3-6B2 BioLegend Cat#103236; RRID:AB_893354 PE Rat anti-Mouse IgD, clone 11-26c.2a BD Pharmingen Cat#558597; RRID:AB_647211 Alexa Fluor® 647 Rat Anti-Mouse T- and B-Cell Activation Antigen, Clone GL7 BD Pharmingen Cat#561529; RRID:AB_10716056 FITC anti-mouse CD95 (Fas) Antibody, clone SA367H8 BioLegend Cat#152606; RRID:AB_2632901 PE anti-mouse CD185 (CXCR5) antibody, clone SPRCL5 eBioscience Cat#12-7185-82; RRID: AB_11217882 APC anti-mouse CD279 (PD-1) Antibody, Clone 29F.1A12 BioLegend Cat#135210; RRID:AB_2159183 Goat Anti-Mouse IgG H&L (HRP) Abcam Cat#ab6789; RRID:AB_955439 Goat Anti-Mouse IgG1 (HRP) Abcam Cat#ab97240; RRID: AB_10695944 Goat Anti-Mouse IgG2a (HRP) Abcam Cat#ab97245; RRID:AB_10680049 Anti-Rabbit IgG (H + L) (HRP) Invitrogen Cat#A16096; RRID:AB_2534770 Goat Anti-Human IgG - H&L (HRP) Abcam Cat#ab6858; RRID:AB_955433 7D11 This paper N/A A27D7 This paper N/A M1H11 Ren et al.51 N/A Bacterial and virus strains DH5α competent cells AlpaLifeBio Cat#KTSM101L Rosetta (DE3) competent cells Tiangen Cat#CB108-02 VACV Tiantan strain Zhang et al.75 N/A VTT-GFP strain Ren et al.51 N/A VACV Western Reserve strain Zuo et al.67 N/A Clade I mpox virus This paper N/A Clade II mpox virus(hMpxV/China/SZSZTH41/2023) Cheng et al.68 N/A Biological samples Serum samples from immunized BALB/c mice This paper N/A Serum samples from immunized New Zealand rabbits This paper N/A MPXV human convalescent sera This paper N/A (Continued on next page) Cell Reports 44, 116358, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins FH002C adjuvant Wu et al.52 N/A Sulfo-NHS-LC-LC-biotin Thermo Fisher Scientific Cat#21338 TMB substrate Tiangen CatPA107-02 BamHI-HF NEB Cat#R3136L NdeI NEB Cat#R0111L XhoI NEB Cat#R0146M Coomassie Brilliant Blue R250 Meilunbio Cat#MB4685 Bovine serum albumin V (BSA V) Biofroxx Cat#4240GR500 Tween 20 Biofroxx Cat#1247ML500 Trehalose Bioss Cat#D10226 TritonTM X-114 Sigma-Aldrich Cat#X114-100ML 2 × Phanta Max Master Mix Vazyme Cat#P515-03 PEI-MAX Polyscience Cat#24765-1 ELISA coated solution (1x) Solarbio Cat#C1050 ELISA Stop Solution Solarbio Cat#C1058 4% polyformaldehyde Yuanye Cat#R20497 Avicel FMC biopolymer Cat#RC-591 10×ACK lysis buffer Elabscience Cat#E-CK-A105 BD GolgiPlugTM Protein Transport Inhibitor BD Pharmingen Cat#555029; RRID: AB_2869014 Leukocyte Activation Cocktail BD Pharmingen Cat#550583; RRID: AB_2868893 Dithiothreitol (DTT) Thermo Fisher Scientific Cat#A39255 Isopropyl b-D-1 thiogalactopyranoside (IPTG) Takara Cat#9030 Standard protein marker Thermo Fisher Scientific Cat#26616 Recombinant A35 protein This paper N/A Recombinant M1 protein This paper N/A Recombinant GvO-DAM protein This paper N/A Recombinant Sd-Ferritin protein This paper N/A Recombinant DAM protein This paper N/A Recombinant A29 protein This paper N/A Recombinant B6 protein This paper N/A Recombinant E8 protein This paper N/A Recombinant Ferritin protein This paper N/A Critical commercial assays Plasmid extraction kit Macherey-Nagel Cat#740412.50 ClonExpress Ultra One Step Cloning Kit Vazyme Cat#C117-02 BCA protein assay kit Thermo Fisher Scientific Cat#A55864 FastPure Viral DNA/RNA Mini Kit Pro Vazyme Cat#RC323-01 2xChamQ Universal SYBR qPCR Master Mix Vazyme Cat#Q711-02 Cytofix/CytopermTM Fixation/ Permeabilization Kit BD Bioscience Cat#554714; RRID: AB_2869008 ToxinSensor Chromogenic LAL Endotoxin Assay Kit GenScript Cat#L00350 Experimental models: Cell lines Expi 293F Thermo Fisher Scientific Cat#A14527 Vero E6 ATCC Cat#CRL1586 (Continued on next page) 18 Cell Reports 44, 116358, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Balb/C mice Guangdong Medical Laboratory Animal Center N/A New Zealand rabbit Guangdong Medical Laboratory Animal Center N/A Oligonucleotides VACV-F 5′-ACATCTGGAGAATCCACAACA-3′ Ren et al.51 N/A VACV-R 5′-CATCATCGGTGGTTGATTTA-3′ Ren et al.51 N/A VACV HA TaqMan probe 5′- FAM-GAGACTCCGGAACCAATTAMRA-3′ Ren et al.51 N/A Recombinant DNA VRC8400-M1R-his This paper N/A VRC8400-B6R-his This paper N/A VRC8400-GVO-DAM-his This paper N/A VRC8400-DAM-his This paper N/A pTO-T7-Sd-Ferritin This paper N/A pTO-T7-Ferritin This paper N/A pTO-T7-A35R-his This paper N/A pTO-T7-A29L-his This paper N/A pTO-T7-E8L-his This paper N/A pCAGGS-7D11(VH)-IgG1 This paper N/A pCAGGS-7D11(VL)-IgG1 This paper N/A pCAGGS-A27D7(VH)-IgG2a This paper N/A pCAGGS-A27D7(VL)-IgG2a This paper N/A Software and algorithms GraphPad Prism V9.5.1 Graphpad https://www.graphpad.com/ SlideViewer 3DHISTECH Ltd. https://www.3dhistech.com/ Tanon 1600 automatic Digital Gel Image Analysis system Tanon https://tanon.cnreagent.com/ Zetasizer Ultra ZS Xplorer V2.0 Malvern Panalytical https://www.malvernpanalytical.com/ PyMol The PyMOL Molecular Graphics System, Version 2.0 Schrö dinger, LLC. https://pymol.org/ FlowJo V10 FlowJo https://www.flowjo.com Octet BLI Analysis 12.2 Fortebio N/A Other Expi 293F serum-free medium OPM Biosciences Cat#AC601501-1000mL Protein A Resin GenScript Cat#L00210-50 Protein G Resin GenScript Cat#L00209-50 Filter paper Cytiva Cat#1004047 Superdex 200 Increase 10/300 GL column Cytiva Cat#28990944 Superose 6 Increase 10/300 GL column Cytiva Cat#29091596 HiLoad 26/600 Superdex 200 pg SEC column Cytiva Cat#28989336 Ni Sepharose excel resin Cytiva Cat#17371203 HiScreen Capto Core 700 column Cytiva Cat#17548115 Capto DEAE column Cytiva Cat#17544310 10K MWCO ultracentrifuge tube Merck millipore Cat#UFC901096 (Continued on next page) Cell Reports 44, 116358, October 28, 2025 19

    Endotoxin Assay:

    Article Title: A single-dose nanoparticle vaccine protects against mpox in preclinical models.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE-CyTM7 Hamster Anti-Mouse CD3e, clone 145-2C11 BD Pharmingen Cat#135210; RRID: AB_2159183 BV510 Rat Anti-Mouse CD4, clone RM4-5 BD Pharmingen Cat#563106; RRID:AB_2687550 PerCP-CyTM5.5 Rat Anti-Mouse CD8a, clone 53–6.7 BD Pharmingen Cat#551162; RRID:AB_394081 Brilliant Violet 421TM anti-mouse IL-4 Antibody, clone 11B11 BioLegend Cat#504120; RRID:AB_2562102 FITC Rat Anti-Mouse IFN-γ, clone XMG1.2 BD Pharmingen Cat#554411; RRID:AB_AB_395375 PE Rat Anti-Mouse TNF, clone MP6-XT22 BD Pharmingen Cat#554419; RRID:AB_395380 Purified Rat Anti-Mouse CD16/CD32, clone 2.4G2 BD Pharmingen Cat#553141; RRID:AB_394656 PerCP/Cyanine5.5 anti-mouse/human CD45R/B220, clone RA3-6B2 BioLegend Cat#103236; RRID:AB_893354 PE Rat anti-Mouse IgD, clone 11-26c.2a BD Pharmingen Cat#558597; RRID:AB_647211 Alexa Fluor® 647 Rat Anti-Mouse T- and B-Cell Activation Antigen, Clone GL7 BD Pharmingen Cat#561529; RRID:AB_10716056 FITC anti-mouse CD95 (Fas) Antibody, clone SA367H8 BioLegend Cat#152606; RRID:AB_2632901 PE anti-mouse CD185 (CXCR5) antibody, clone SPRCL5 eBioscience Cat#12-7185-82; RRID: AB_11217882 APC anti-mouse CD279 (PD-1) Antibody, Clone 29F.1A12 BioLegend Cat#135210; RRID:AB_2159183 Goat Anti-Mouse IgG H&L (HRP) Abcam Cat#ab6789; RRID:AB_955439 Goat Anti-Mouse IgG1 (HRP) Abcam Cat#ab97240; RRID: AB_10695944 Goat Anti-Mouse IgG2a (HRP) Abcam Cat#ab97245; RRID:AB_10680049 Anti-Rabbit IgG (H + L) (HRP) Invitrogen Cat#A16096; RRID:AB_2534770 Goat Anti-Human IgG - H&L (HRP) Abcam Cat#ab6858; RRID:AB_955433 7D11 This paper N/A A27D7 This paper N/A M1H11 Ren et al.51 N/A Bacterial and virus strains DH5α competent cells AlpaLifeBio Cat#KTSM101L Rosetta (DE3) competent cells Tiangen Cat#CB108-02 VACV Tiantan strain Zhang et al.75 N/A VTT-GFP strain Ren et al.51 N/A VACV Western Reserve strain Zuo et al.67 N/A Clade I mpox virus This paper N/A Clade II mpox virus(hMpxV/China/SZSZTH41/2023) Cheng et al.68 N/A Biological samples Serum samples from immunized BALB/c mice This paper N/A Serum samples from immunized New Zealand rabbits This paper N/A MPXV human convalescent sera This paper N/A (Continued on next page) Cell Reports 44, 116358, October 28, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins FH002C adjuvant Wu et al.52 N/A Sulfo-NHS-LC-LC-biotin Thermo Fisher Scientific Cat#21338 TMB substrate Tiangen CatPA107-02 BamHI-HF NEB Cat#R3136L NdeI NEB Cat#R0111L XhoI NEB Cat#R0146M Coomassie Brilliant Blue R250 Meilunbio Cat#MB4685 Bovine serum albumin V (BSA V) Biofroxx Cat#4240GR500 Tween 20 Biofroxx Cat#1247ML500 Trehalose Bioss Cat#D10226 TritonTM X-114 Sigma-Aldrich Cat#X114-100ML 2 × Phanta Max Master Mix Vazyme Cat#P515-03 PEI-MAX Polyscience Cat#24765-1 ELISA coated solution (1x) Solarbio Cat#C1050 ELISA Stop Solution Solarbio Cat#C1058 4% polyformaldehyde Yuanye Cat#R20497 Avicel FMC biopolymer Cat#RC-591 10×ACK lysis buffer Elabscience Cat#E-CK-A105 BD GolgiPlugTM Protein Transport Inhibitor BD Pharmingen Cat#555029; RRID: AB_2869014 Leukocyte Activation Cocktail BD Pharmingen Cat#550583; RRID: AB_2868893 Dithiothreitol (DTT) Thermo Fisher Scientific Cat#A39255 Isopropyl b-D-1 thiogalactopyranoside (IPTG) Takara Cat#9030 Standard protein marker Thermo Fisher Scientific Cat#26616 Recombinant A35 protein This paper N/A Recombinant M1 protein This paper N/A Recombinant GvO-DAM protein This paper N/A Recombinant Sd-Ferritin protein This paper N/A Recombinant DAM protein This paper N/A Recombinant A29 protein This paper N/A Recombinant B6 protein This paper N/A Recombinant E8 protein This paper N/A Recombinant Ferritin protein This paper N/A Critical commercial assays Plasmid extraction kit Macherey-Nagel Cat#740412.50 ClonExpress Ultra One Step Cloning Kit Vazyme Cat#C117-02 BCA protein assay kit Thermo Fisher Scientific Cat#A55864 FastPure Viral DNA/RNA Mini Kit Pro Vazyme Cat#RC323-01 2xChamQ Universal SYBR qPCR Master Mix Vazyme Cat#Q711-02 Cytofix/CytopermTM Fixation/ Permeabilization Kit BD Bioscience Cat#554714; RRID: AB_2869008 ToxinSensor Chromogenic LAL Endotoxin Assay Kit GenScript Cat#L00350 Experimental models: Cell lines Expi 293F Thermo Fisher Scientific Cat#A14527 Vero E6 ATCC Cat#CRL1586 (Continued on next page) 18 Cell Reports 44, 116358, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Balb/C mice Guangdong Medical Laboratory Animal Center N/A New Zealand rabbit Guangdong Medical Laboratory Animal Center N/A Oligonucleotides VACV-F 5′-ACATCTGGAGAATCCACAACA-3′ Ren et al.51 N/A VACV-R 5′-CATCATCGGTGGTTGATTTA-3′ Ren et al.51 N/A VACV HA TaqMan probe 5′- FAM-GAGACTCCGGAACCAATTAMRA-3′ Ren et al.51 N/A Recombinant DNA VRC8400-M1R-his This paper N/A VRC8400-B6R-his This paper N/A VRC8400-GVO-DAM-his This paper N/A VRC8400-DAM-his This paper N/A pTO-T7-Sd-Ferritin This paper N/A pTO-T7-Ferritin This paper N/A pTO-T7-A35R-his This paper N/A pTO-T7-A29L-his This paper N/A pTO-T7-E8L-his This paper N/A pCAGGS-7D11(VH)-IgG1 This paper N/A pCAGGS-7D11(VL)-IgG1 This paper N/A pCAGGS-A27D7(VH)-IgG2a This paper N/A pCAGGS-A27D7(VL)-IgG2a This paper N/A Software and algorithms GraphPad Prism V9.5.1 Graphpad https://www.graphpad.com/ SlideViewer 3DHISTECH Ltd. https://www.3dhistech.com/ Tanon 1600 automatic Digital Gel Image Analysis system Tanon https://tanon.cnreagent.com/ Zetasizer Ultra ZS Xplorer V2.0 Malvern Panalytical https://www.malvernpanalytical.com/ PyMol The PyMOL Molecular Graphics System, Version 2.0 Schrö dinger, LLC. https://pymol.org/ FlowJo V10 FlowJo https://www.flowjo.com Octet BLI Analysis 12.2 Fortebio N/A Other Expi 293F serum-free medium OPM Biosciences Cat#AC601501-1000mL Protein A Resin GenScript Cat#L00210-50 Protein G Resin GenScript Cat#L00209-50 Filter paper Cytiva Cat#1004047 Superdex 200 Increase 10/300 GL column Cytiva Cat#28990944 Superose 6 Increase 10/300 GL column Cytiva Cat#29091596 HiLoad 26/600 Superdex 200 pg SEC column Cytiva Cat#28989336 Ni Sepharose excel resin Cytiva Cat#17371203 HiScreen Capto Core 700 column Cytiva Cat#17548115 Capto DEAE column Cytiva Cat#17544310 10K MWCO ultracentrifuge tube Merck millipore Cat#UFC901096 (Continued on next page) Cell Reports 44, 116358, October 28, 2025 19



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