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tmb developing reagent  (Biosynth Carbosynth)


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    Biosynth Carbosynth tmb developing reagent
    Tmb Developing Reagent, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tmb+developing+reagent/Tetramethylbenzidine/pmc11175361-57-13-16
    Average 91 stars, based on 2 article reviews
    tmb developing reagent - by Bioz Stars, 2026-11
    91/100 stars

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    other:

    Article Title: Targeting of TAM Receptors Ameliorates Fibrotic Mechanisms in Idiopathic Pulmonary Fibrosis
    Article Snippet: After incubation, cells were washed and incubated with an HRP conjugated rabbit anti-mouse antibody (1:5000 diluted, R&D systems) for 30 min at room temperature.

    Incubation:

    Article Title: Human-monoclonal-antibody therapy protects nonhuman primates against advanced Lassa fever.
    Article Snippet: Enzyme-linked immunosorbent assays (ELISA) using recombinant LASV proteins (NP; Genbank HQ688672), which were optimized for the detection of virus-specific IgM and IgG levels in serum, were coated in strip-well plates, blocked, dried, and packaged with desiccating packs (Zalgen Labs; 10004 ReLASVTM Pan-Lassa IgG/IgM ELISA Test Kit)20. .. For analysis, primate sera were diluted 1:100 in a proprietary-sample dilution buffer and incubated in wells for 30 min at room temperature, washed, and then incubated with a 1:2,500 dilution of HRPlabeled anti-monkey IgG (Fitzgerald Industries International, 43R-IG020HRP) for an additional 30 min. After washing, detection was performed with tetramethylbenzidine substrate for 10 min at room temperature, stopped with sulfuric acid, and then read at an absorbance of 450 nm using a Molecular Devices ELISA plate reader. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Human-monoclonal-antibody therapy protects nonhuman primates against advanced Lassa fever.
    Article Snippet: Enzyme-linked immunosorbent assays (ELISA) using recombinant LASV proteins (NP; Genbank HQ688672), which were optimized for the detection of virus-specific IgM and IgG levels in serum, were coated in strip-well plates, blocked, dried, and packaged with desiccating packs (Zalgen Labs; 10004 ReLASVTM Pan-Lassa IgG/IgM ELISA Test Kit)20. .. For analysis, primate sera were diluted 1:100 in a proprietary-sample dilution buffer and incubated in wells for 30 min at room temperature, washed, and then incubated with a 1:2,500 dilution of HRPlabeled anti-monkey IgG (Fitzgerald Industries International, 43R-IG020HRP) for an additional 30 min. After washing, detection was performed with tetramethylbenzidine substrate for 10 min at room temperature, stopped with sulfuric acid, and then read at an absorbance of 450 nm using a Molecular Devices ELISA plate reader. ..



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    Image Search Results


    A Representative scheme of our homemade ELISA test, i.e. ELEXO, including EV-coated microtiter plate, primary antibody, HRP-conjugated secondary antibody and colorimetric reaction with TMB substrate. B H1975 and H1299 EVs analyzed by ELEXO assay. IgG isotype and Phosphate-Buffered Saline (PBS) were used as internal controls. Data are reported as mean and SD ( n = 3) of arbitrary units of O.D. (Optical Density) at the specified wavelength (nm) (* p = 0.02). C , D Representative images of SEM ( C ) and TEM ( D ) analysis of EVs isolated from the sera of prostate cancer patients. Images are representative of pivotal and training cohort PCas. E Box plot representative of serum EV size distribution evaluated by SEM analysis. F , G ELEXO measurement of EpCAM ( F ) and PD-L1 ( G ) antigens in EVs isolated from colon and lung cancer patients, respectively. CD81 has been used as endogenous EV control. Data are reported as mean and SD ( n = 3) of arbitrary units of O.D. (Optical Density) at the specified wavelength (nm). Internal reference controls were reported as IgG, CD-81, EpCAM, or PD-L1 antibody in PBS condition. H Absolute RPPA quantification of EGFR_pY1068 synthetic peptide in dilution curve along with isolated EVs from sera of colon and lung cancer patients. The expression levels in EVs fall below the lowest EGFR_pY1068 peptide dilution point and their scale is magnified in the left panel of the plot. The reference curve in the lower panel was obtained as in Fig. to predict absolute EGFR_pY1068 RPPA levels in EV samples.

    Journal: Cell Death & Disease

    Article Title: Diagnostic and prognostic potential of the proteomic profiling of serum-derived extracellular vesicles in prostate cancer

    doi: 10.1038/s41419-021-03909-z

    Figure Lengend Snippet: A Representative scheme of our homemade ELISA test, i.e. ELEXO, including EV-coated microtiter plate, primary antibody, HRP-conjugated secondary antibody and colorimetric reaction with TMB substrate. B H1975 and H1299 EVs analyzed by ELEXO assay. IgG isotype and Phosphate-Buffered Saline (PBS) were used as internal controls. Data are reported as mean and SD ( n = 3) of arbitrary units of O.D. (Optical Density) at the specified wavelength (nm) (* p = 0.02). C , D Representative images of SEM ( C ) and TEM ( D ) analysis of EVs isolated from the sera of prostate cancer patients. Images are representative of pivotal and training cohort PCas. E Box plot representative of serum EV size distribution evaluated by SEM analysis. F , G ELEXO measurement of EpCAM ( F ) and PD-L1 ( G ) antigens in EVs isolated from colon and lung cancer patients, respectively. CD81 has been used as endogenous EV control. Data are reported as mean and SD ( n = 3) of arbitrary units of O.D. (Optical Density) at the specified wavelength (nm). Internal reference controls were reported as IgG, CD-81, EpCAM, or PD-L1 antibody in PBS condition. H Absolute RPPA quantification of EGFR_pY1068 synthetic peptide in dilution curve along with isolated EVs from sera of colon and lung cancer patients. The expression levels in EVs fall below the lowest EGFR_pY1068 peptide dilution point and their scale is magnified in the left panel of the plot. The reference curve in the lower panel was obtained as in Fig. to predict absolute EGFR_pY1068 RPPA levels in EV samples.

    Article Snippet: After washing, the color-developing reaction with TMB (Thermoscientific, Massachusetts, USA) was started and then stopped with H2SO4.

    Techniques: Enzyme-linked Immunosorbent Assay, Saline, Isolation, Control, Expressing