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Boehringer Mannheim tmb substrate solution tetramethylbenzidine (tmb)
Tmb Substrate Solution Tetramethylbenzidine (Tmb), supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tmb+(tetramethylbenzidine)+solution/tmb+substrate+solution+tetramethylbenzidine++tmb+/us07071332-1874-3-5
Average 90 stars, based on 1 article reviews
tmb substrate solution tetramethylbenzidine (tmb) - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

BrdU Incorporation Assay:

Article Title: Tyrosine kinase inhibitors
Article Snippet: .. Cellular/Biologic Assays Assay 1: PDGF-Induced BrdU Incorporation Assay Materials and Reagents: (1) PDGF: human PDGF B/B; 1276-956, Boehringer Mannheim, Germany. (2) BrdU Labeling Reagent: 10 mM, in PBS (pH7.4), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (3) FixDenat: fixation solution (ready to use), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (4) Anti-BrdU-POD: mouse monoclonal antibody conjugated with peroxidase, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (5) TMB Substrate Solution: tetramethylbenzidine (TMB), ready to use, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (6) PBS Washing Solution: 1× PBS, pH 7.4, made in house (Sugen, Inc., Redwood City, Calif.). (7) Albumin, Bovine (BSA): fraction V powder; A-8551, Sigma Chemical Co., USA. (8) 3T3 cell line genetically engineered to express human PDGF-R. Protocol (1) Cells are seeded at 8000 cells/well in DMEM, 10% CS, 2 mM Gln in a 96 well plate. ..

Article Title: Pyrrole substituted 2-indolinone protein kinase inhibitors
Article Snippet: .. Cellular/Biologic Assays PDGF-Induced BrdU Incorporation Assay Materials and Reagents: (1) PDGF: human PDGF B/B, 1276-956, Boehringer Mannheim, Germany. (2) BrdU Labeling Reagent: 10 mM, in PBS (pH7.4), Cat. No. 1 647 229, Boehringer Mannheim, Germany.. (3) FixDenat: fixation solution (ready to use), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (4) Anti-BrdU-POD: mouse monoclonal antibody conjugated with peroxidase, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (5) TMB Substrate Solution: tetramethylbenzidine (TMB), ready to use, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (6) PBS Washing Solution : 1X PBS, pH 7.4 (Sugen, Inc., Redwood City, Calif.). (7) Albumin, Bovine (BSA): fraction V powder, A-8551, Sigma Chemical Co., USA. (8) 3T3 cell line genetically engineered to express human PDGF-R. Protocol (1) Cells are seeded at 8000 cells/well in DMEM, 10% CS, 2 mM Gln in a 96 well plate. .. Cellular/Biologic Assays PDGF-Induced BrdU Incorporation Assay Materials and Reagents: (1) PDGF: human PDGF B/B, 1276-956, Boehringer Mannheim, Germany. (2) BrdU Labeling Reagent: 10 mM, in PBS (pH7.4), Cat. No. 1 647 229, Boehringer Mannheim, Germany.. (3) FixDenat: fixation solution (ready to use), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (4) Anti-BrdU-POD: mouse monoclonal antibody conjugated with peroxidase, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (5) TMB Substrate Solution: tetramethylbenzidine (TMB), ready to use, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (6) PBS Washing Solution : 1X PBS, pH 7.4 (Sugen, Inc., Redwood City, Calif.). (7) Albumin, Bovine (BSA): fraction V powder, A-8551, Sigma Chemical Co., USA. (8) 3T3 cell line genetically engineered to express human PDGF-R. Protocol (1) Cells are seeded at 8000 cells/well in DMEM, 10% CS, 2 mM Gln in a 96 well plate.

Article Title: Pyrrole substituted 2-indolinone protein kinase inhibitors
Article Snippet: IGF1-Induced BrdU Incorporation Assay Materials and Reagents (1) IGF1 Ligand: human, recombinant, G511, Promega Corp, USA. (2) BrdU Labeling Reagent: 10 mM, in PBS (pH7.4), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (3) FixDenat: fixation solution (ready to use), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (4) Anti-BrdU-POD: mouse monoclonal antibody conjugated with peroxidase, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (5) TMB Substrate Solution: tetramethylbenzidine (TMB), ready to use, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (6) PBS Washing Solution: 1X PBS, pH 7.4 (Sugen, Inc., Redwood City, Calif.). (7) Albumin, Bovine (BSA): fraction V powder, A-8551, Sigma Chemical Co., USA. (8) 3T3 cell line genetically engineered to express human IGF-1 receptor. .. IGF1-Induced BrdU Incorporation Assay Materials and Reagents (1) IGF1 Ligand: human, recombinant, G511, Promega Corp, USA. (2) BrdU Labeling Reagent: 10 mM, in PBS (pH7.4), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (3) FixDenat: fixation solution (ready to use), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (4) Anti-BrdU-POD: mouse monoclonal antibody conjugated with peroxidase, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (5) TMB Substrate Solution: tetramethylbenzidine (TMB), ready to use, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (6) PBS Washing Solution: 1X PBS, pH 7.4 (Sugen, Inc., Redwood City, Calif.). (7) Albumin, Bovine (BSA): fraction V powder, A-8551, Sigma Chemical Co., USA. (8) 3T3 cell line genetically engineered to express human IGF-1 receptor. ..

Article Title: Bicyclic protein kinase inhibitors
Article Snippet: .. Assay 4: IGF1-Induced BrdU Incorporation Assay Materials and Reagents: (1) IGF1 Ligand: human, recombinant; G511, Promega Corp, USA. (2) BrdU Labeling Reagent: 10 mM, in PBS (pH7.4), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (3) FixDenat: fixation solution (ready to use), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (4) Anti-BrdU-POD: mouse monoclonal antibody conjugated with peroxidase, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (5) TMB Substrate Solution: tetramethylbenzidine (TMB), ready to use, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (6) PBS Washing Solution: 1× PBS, pH 7.4, made in house (Sugen, Inc., Redwood City, Calif.). (7) Albumin, Bovine (BSA): fraction V powder; A-8551, Sigma Chemical Co., USA. (8) 3T3 cell line genetically engineered to express human IGF-1 receptor. ..

Labeling:

Article Title: Tyrosine kinase inhibitors
Article Snippet: .. Cellular/Biologic Assays Assay 1: PDGF-Induced BrdU Incorporation Assay Materials and Reagents: (1) PDGF: human PDGF B/B; 1276-956, Boehringer Mannheim, Germany. (2) BrdU Labeling Reagent: 10 mM, in PBS (pH7.4), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (3) FixDenat: fixation solution (ready to use), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (4) Anti-BrdU-POD: mouse monoclonal antibody conjugated with peroxidase, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (5) TMB Substrate Solution: tetramethylbenzidine (TMB), ready to use, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (6) PBS Washing Solution: 1× PBS, pH 7.4, made in house (Sugen, Inc., Redwood City, Calif.). (7) Albumin, Bovine (BSA): fraction V powder; A-8551, Sigma Chemical Co., USA. (8) 3T3 cell line genetically engineered to express human PDGF-R. Protocol (1) Cells are seeded at 8000 cells/well in DMEM, 10% CS, 2 mM Gln in a 96 well plate. ..

Article Title: Pyrrole substituted 2-indolinone protein kinase inhibitors
Article Snippet: .. Cellular/Biologic Assays PDGF-Induced BrdU Incorporation Assay Materials and Reagents: (1) PDGF: human PDGF B/B, 1276-956, Boehringer Mannheim, Germany. (2) BrdU Labeling Reagent: 10 mM, in PBS (pH7.4), Cat. No. 1 647 229, Boehringer Mannheim, Germany.. (3) FixDenat: fixation solution (ready to use), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (4) Anti-BrdU-POD: mouse monoclonal antibody conjugated with peroxidase, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (5) TMB Substrate Solution: tetramethylbenzidine (TMB), ready to use, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (6) PBS Washing Solution : 1X PBS, pH 7.4 (Sugen, Inc., Redwood City, Calif.). (7) Albumin, Bovine (BSA): fraction V powder, A-8551, Sigma Chemical Co., USA. (8) 3T3 cell line genetically engineered to express human PDGF-R. Protocol (1) Cells are seeded at 8000 cells/well in DMEM, 10% CS, 2 mM Gln in a 96 well plate. .. Cellular/Biologic Assays PDGF-Induced BrdU Incorporation Assay Materials and Reagents: (1) PDGF: human PDGF B/B, 1276-956, Boehringer Mannheim, Germany. (2) BrdU Labeling Reagent: 10 mM, in PBS (pH7.4), Cat. No. 1 647 229, Boehringer Mannheim, Germany.. (3) FixDenat: fixation solution (ready to use), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (4) Anti-BrdU-POD: mouse monoclonal antibody conjugated with peroxidase, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (5) TMB Substrate Solution: tetramethylbenzidine (TMB), ready to use, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (6) PBS Washing Solution : 1X PBS, pH 7.4 (Sugen, Inc., Redwood City, Calif.). (7) Albumin, Bovine (BSA): fraction V powder, A-8551, Sigma Chemical Co., USA. (8) 3T3 cell line genetically engineered to express human PDGF-R. Protocol (1) Cells are seeded at 8000 cells/well in DMEM, 10% CS, 2 mM Gln in a 96 well plate.

Article Title: Pyrrole substituted 2-indolinone protein kinase inhibitors
Article Snippet: IGF1-Induced BrdU Incorporation Assay Materials and Reagents (1) IGF1 Ligand: human, recombinant, G511, Promega Corp, USA. (2) BrdU Labeling Reagent: 10 mM, in PBS (pH7.4), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (3) FixDenat: fixation solution (ready to use), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (4) Anti-BrdU-POD: mouse monoclonal antibody conjugated with peroxidase, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (5) TMB Substrate Solution: tetramethylbenzidine (TMB), ready to use, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (6) PBS Washing Solution: 1X PBS, pH 7.4 (Sugen, Inc., Redwood City, Calif.). (7) Albumin, Bovine (BSA): fraction V powder, A-8551, Sigma Chemical Co., USA. (8) 3T3 cell line genetically engineered to express human IGF-1 receptor. .. IGF1-Induced BrdU Incorporation Assay Materials and Reagents (1) IGF1 Ligand: human, recombinant, G511, Promega Corp, USA. (2) BrdU Labeling Reagent: 10 mM, in PBS (pH7.4), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (3) FixDenat: fixation solution (ready to use), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (4) Anti-BrdU-POD: mouse monoclonal antibody conjugated with peroxidase, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (5) TMB Substrate Solution: tetramethylbenzidine (TMB), ready to use, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (6) PBS Washing Solution: 1X PBS, pH 7.4 (Sugen, Inc., Redwood City, Calif.). (7) Albumin, Bovine (BSA): fraction V powder, A-8551, Sigma Chemical Co., USA. (8) 3T3 cell line genetically engineered to express human IGF-1 receptor. ..

Article Title: Bicyclic protein kinase inhibitors
Article Snippet: .. Assay 4: IGF1-Induced BrdU Incorporation Assay Materials and Reagents: (1) IGF1 Ligand: human, recombinant; G511, Promega Corp, USA. (2) BrdU Labeling Reagent: 10 mM, in PBS (pH7.4), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (3) FixDenat: fixation solution (ready to use), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (4) Anti-BrdU-POD: mouse monoclonal antibody conjugated with peroxidase, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (5) TMB Substrate Solution: tetramethylbenzidine (TMB), ready to use, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (6) PBS Washing Solution: 1× PBS, pH 7.4, made in house (Sugen, Inc., Redwood City, Calif.). (7) Albumin, Bovine (BSA): fraction V powder; A-8551, Sigma Chemical Co., USA. (8) 3T3 cell line genetically engineered to express human IGF-1 receptor. ..

Recombinant:

Article Title: Pyrrole substituted 2-indolinone protein kinase inhibitors
Article Snippet: IGF1-Induced BrdU Incorporation Assay Materials and Reagents (1) IGF1 Ligand: human, recombinant, G511, Promega Corp, USA. (2) BrdU Labeling Reagent: 10 mM, in PBS (pH7.4), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (3) FixDenat: fixation solution (ready to use), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (4) Anti-BrdU-POD: mouse monoclonal antibody conjugated with peroxidase, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (5) TMB Substrate Solution: tetramethylbenzidine (TMB), ready to use, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (6) PBS Washing Solution: 1X PBS, pH 7.4 (Sugen, Inc., Redwood City, Calif.). (7) Albumin, Bovine (BSA): fraction V powder, A-8551, Sigma Chemical Co., USA. (8) 3T3 cell line genetically engineered to express human IGF-1 receptor. .. IGF1-Induced BrdU Incorporation Assay Materials and Reagents (1) IGF1 Ligand: human, recombinant, G511, Promega Corp, USA. (2) BrdU Labeling Reagent: 10 mM, in PBS (pH7.4), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (3) FixDenat: fixation solution (ready to use), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (4) Anti-BrdU-POD: mouse monoclonal antibody conjugated with peroxidase, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (5) TMB Substrate Solution: tetramethylbenzidine (TMB), ready to use, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (6) PBS Washing Solution: 1X PBS, pH 7.4 (Sugen, Inc., Redwood City, Calif.). (7) Albumin, Bovine (BSA): fraction V powder, A-8551, Sigma Chemical Co., USA. (8) 3T3 cell line genetically engineered to express human IGF-1 receptor. ..

Article Title: Bicyclic protein kinase inhibitors
Article Snippet: .. Assay 4: IGF1-Induced BrdU Incorporation Assay Materials and Reagents: (1) IGF1 Ligand: human, recombinant; G511, Promega Corp, USA. (2) BrdU Labeling Reagent: 10 mM, in PBS (pH7.4), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (3) FixDenat: fixation solution (ready to use), Cat. No. 1 647 229, Boehringer Mannheim, Germany. (4) Anti-BrdU-POD: mouse monoclonal antibody conjugated with peroxidase, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (5) TMB Substrate Solution: tetramethylbenzidine (TMB), ready to use, Cat. No. 1 647 229, Boehringer Mannheim, Germany. (6) PBS Washing Solution: 1× PBS, pH 7.4, made in house (Sugen, Inc., Redwood City, Calif.). (7) Albumin, Bovine (BSA): fraction V powder; A-8551, Sigma Chemical Co., USA. (8) 3T3 cell line genetically engineered to express human IGF-1 receptor. ..

other:

Article Title: (2-Oxindol-3-ylidenyl) acetic acid derivatives and their use as protein kinase inhibitors
Article Snippet: TMB Substrate Solution: tetramethylbenzidine (TMB, Boehringer Mannheim, Germany).



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