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fluorogenic substrate ac thz tle leu glnmca  (Peptide Institute)

 
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    Structured Review

    Peptide Institute fluorogenic substrate ac thz tle leu glnmca
    Fluorogenic Substrate Ac Thz Tle Leu Glnmca, supplied by Peptide Institute, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tle/acetyl+aminomethylcoumarin+asp+fluorogenic+glu+substrate+valasp/pm41107840-70-8-11
    Average 86 stars, based on 1 article reviews
    fluorogenic substrate ac thz tle leu glnmca - by Bioz Stars, 2026-10
    86/100 stars

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    Activity Assay:

    Article Title: Yersinia enterocolitica YopP-induced Apoptosis of Macrophages Involves the Apoptotic Signaling Cascade Upstream of Bid
    Article Snippet: .. Caspase activity was determined by incubation of cell lysates (containing 40 mg of total protein) with 50 mM of the fluorogenic substrate acetyl-Asp-Glu-ValAsp-aminomethylcoumarin (Ac-DEVD-amc) (Peptide Institute Inc., Osaka, Japan) in 150 ml of cell-free system buffer, comprising 10 mM Hepes, pH 7.4, 220 mM mannitol, 68 mM sucrose, 2 mM NaCl, 2.5 mM KH2PO4, 0.5 mM EGTA, 2 mM MgCl2, 5 mM pyruvate, 0.1 mM phenylmethylsulfonyl fluoride, and 10 mM dithiothreitol (13). .. The release of fluorescent aminomethylcoumarin was measured by fluorometry and expressed as fluorescence increase per min (DF/min). (Cytoflor; PerSeptive Biosystems, Cambridge, MA).

    Incubation:

    Article Title: Yersinia enterocolitica YopP-induced Apoptosis of Macrophages Involves the Apoptotic Signaling Cascade Upstream of Bid
    Article Snippet: .. Caspase activity was determined by incubation of cell lysates (containing 40 mg of total protein) with 50 mM of the fluorogenic substrate acetyl-Asp-Glu-ValAsp-aminomethylcoumarin (Ac-DEVD-amc) (Peptide Institute Inc., Osaka, Japan) in 150 ml of cell-free system buffer, comprising 10 mM Hepes, pH 7.4, 220 mM mannitol, 68 mM sucrose, 2 mM NaCl, 2.5 mM KH2PO4, 0.5 mM EGTA, 2 mM MgCl2, 5 mM pyruvate, 0.1 mM phenylmethylsulfonyl fluoride, and 10 mM dithiothreitol (13). .. The release of fluorescent aminomethylcoumarin was measured by fluorometry and expressed as fluorescence increase per min (DF/min). (Cytoflor; PerSeptive Biosystems, Cambridge, MA).



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