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Structured Review

Nikon tirf module
Focal adhesion distribution and cell stiffness. (A) <t>TIRF-based</t> investigation of hippocampal cells on glass control (left column), pSLG (central column) and fSLG (right column). Micrographs in the first row show cells stained against β-tubulin III (in red, acquired as <t>an</t> <t>epi-fluorescence</t> signal), those in the second row show cells labeled against vinculin (in green, acquired as a TIRF signal). Scale bars: 20 μm. (B) Bar plot summarizing vinculin puncta density across the three conditions. Significant differences were detected. (C) Bar plot showing vinculin-positive puncta size. Statistically significant differences emerged between the three experimental groups. (D) Box plot depicting cell stiffnesses measured on neurons grown above the three examined substrates. A significant reduction in cell stiffness was found in the case of fSLG-interfaced cells. Significance: * p < 0.05, ** p < 0.01, *** p < 0.001.
Tirf Module, supplied by Nikon, used in various techniques. Bioz Stars score: 95/100, based on 311 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tiu+microscope/Maximum+Magnification+Module/pmc08788235-100-32-34
Average 95 stars, based on 311 article reviews
tirf module - by Bioz Stars, 2026-09
95/100 stars

Images

1) Product Images from "Bidirectional Modulation of Neuronal Cells Electrical and Mechanical Properties Through Pristine and Functionalized Graphene Substrates"

Article Title: Bidirectional Modulation of Neuronal Cells Electrical and Mechanical Properties Through Pristine and Functionalized Graphene Substrates

Journal: Frontiers in Neuroscience

doi: 10.3389/fnins.2021.811348

Focal adhesion distribution and cell stiffness. (A) TIRF-based investigation of hippocampal cells on glass control (left column), pSLG (central column) and fSLG (right column). Micrographs in the first row show cells stained against β-tubulin III (in red, acquired as an epi-fluorescence signal), those in the second row show cells labeled against vinculin (in green, acquired as a TIRF signal). Scale bars: 20 μm. (B) Bar plot summarizing vinculin puncta density across the three conditions. Significant differences were detected. (C) Bar plot showing vinculin-positive puncta size. Statistically significant differences emerged between the three experimental groups. (D) Box plot depicting cell stiffnesses measured on neurons grown above the three examined substrates. A significant reduction in cell stiffness was found in the case of fSLG-interfaced cells. Significance: * p < 0.05, ** p < 0.01, *** p < 0.001.
Figure Legend Snippet: Focal adhesion distribution and cell stiffness. (A) TIRF-based investigation of hippocampal cells on glass control (left column), pSLG (central column) and fSLG (right column). Micrographs in the first row show cells stained against β-tubulin III (in red, acquired as an epi-fluorescence signal), those in the second row show cells labeled against vinculin (in green, acquired as a TIRF signal). Scale bars: 20 μm. (B) Bar plot summarizing vinculin puncta density across the three conditions. Significant differences were detected. (C) Bar plot showing vinculin-positive puncta size. Statistically significant differences emerged between the three experimental groups. (D) Box plot depicting cell stiffnesses measured on neurons grown above the three examined substrates. A significant reduction in cell stiffness was found in the case of fSLG-interfaced cells. Significance: * p < 0.05, ** p < 0.01, *** p < 0.001.

Techniques Used: Control, Staining, Fluorescence, Labeling

Related Articles

Microscopy:

Article Title: Methods for multiplex imaging using labeled nucleic acid imaging agents
Article Snippet: .. Samples were loaded onto an inverted Nikon Eclipse Ti microscope (Nikon Instruments) with a fluorescence module and an Andor Zyla sCMOS camera. ..

Article Title: Methods for multiplex imaging using labeled nucleic acid imaging agents
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Article Title: Enhancing Lateral Resolution Using Two-Colour Direct Stochastic Optical Reconstruction Microscopy to Unravel Synaptic Tau Pathology in Alzheimer's Disease.
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Fluorescence:

Article Title: Methods for multiplex imaging using labeled nucleic acid imaging agents
Article Snippet: .. Samples were loaded onto an inverted Nikon Eclipse Ti microscope (Nikon Instruments) with a fluorescence module and an Andor Zyla sCMOS camera. ..

Article Title: Methods for multiplex imaging using labeled nucleic acid imaging agents
Article Snippet: .. Samples were imaged using an inverted Nikon Eclipse Ti microscope (Nikon Instruments) with a fluorescence module and an Andor Zyla sCMOS camera. ..

Software:

Article Title: Amylin exacerbates tau pathology in the visual cortex of diabetic mice by impairing lysosomal activity
Article Snippet: .. High-magnification z-stack images 265 were converted to maximum intensity 2D projections and deconvolved in NIS-Elements 266 software (Nikon) 52. ..

Article Title: Enhancing Lateral Resolution Using Two-Colour Direct Stochastic Optical Reconstruction Microscopy to Unravel Synaptic Tau Pathology in Alzheimer's Disease.
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Imaging:

Article Title: Enhancing Lateral Resolution Using Two-Colour Direct Stochastic Optical Reconstruction Microscopy to Unravel Synaptic Tau Pathology in Alzheimer's Disease.
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Immunofluorescence:

Article Title: Enhancing Lateral Resolution Using Two-Colour Direct Stochastic Optical Reconstruction Microscopy to Unravel Synaptic Tau Pathology in Alzheimer's Disease.
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Activation Assay:

Article Title: Enhancing Lateral Resolution Using Two-Colour Direct Stochastic Optical Reconstruction Microscopy to Unravel Synaptic Tau Pathology in Alzheimer's Disease.
Article Snippet: Aims: In Alzheimer's disease (AD), the pathological accumulation of tau in synapses contributes to synapse dysfunction and loss.. However, the small and complex structure of synapses limits the investigation when using conventional techniques.. In this work, we describe the combination of array tomography (AT) with twocolour direct stochastic optical reconstruction microscopy (dSTORM) to enhance lateral resolution for resolving synaptic terminals in human postmortem brain.



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