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Structured Review

ViraQuest Inc ad5cmv(gfp
Ad5cmv(gfp, supplied by ViraQuest Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue-specific+patterns+of+expression/pvqad+cmv+k+npa/pm23936314-251-8-14
Average 90 stars, based on 1 article reviews
ad5cmv(gfp - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Over Expression:

Article Title: Mechanism of dysfunction of human variants of the IRAK4 kinase and a role for its kinase activity in interleukin-1 receptor signaling
Article Snippet: IRAK4 constructs of the WT, D329A, and R12C compound variant containing C-terminal FLAG tag were cloned into pVQAd CMV K-NpA vector (Viraquest, North Liberty, IN).

Article Title: Genetic dissection of the miR-200–Zeb1 axis reveals its importance in tumor differentiation and invasion
Article Snippet: Fragments were cloned downstream of a CMV promoter in pVQAd CMV K-NpA for recombinant adenovirus production (ViraQuest).

Article Title: In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion
Article Snippet: Mouse Myc-DKK tagged cDNAs of Tial1 (NM_00009383) were provided by Origene and cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites KpnI and PmeI (Ad-Tial1).

Article Title: High density lipoprotein binding protein (Hdlbp/Vigilin) iRNA compositions and methods of use thereof
Article Snippet: Adenoviral Infections The sequence of V5-tagged hVigilin was cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites BamHI and XhoI (NEB).

Article Title: SorCS1 for the treatment of obesity
Article Snippet: TABLE 2 Sequence identity to human SorCS1 Protein DNA Species (% identity) (% identity) Human 100 100 Chimpanzee 99.6 99.4 Dog 97.6 92.5 Cow 92.9 89.8 Mouse 93.2 87.7 Rat 93.2 88.0 Chicken 85.3 79.7 FIGS. 17A-17B: Decreased plasma glucose levels in female wild-type and SorCS1 knockout mice after hepatic overexpression of soluble SorCS1.

Article Title: Inflammation-induced TRIM21 represses hepatic steatosis by promoting the ubiquitination of lipogenic regulators
Article Snippet: For TRIM21 overexpression, the human TRIM21 cDNA (NM_003141) tagged by Myc-DDK was provided by Origene (RC202088) and subcloned under the CMV promoter into the adenovirus expression vector pVQAd CMV K-NpA from Viraquest (Ad-Trim21).

Article Title: Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches
Article Snippet: hBMSC culture medium: α-MEM, 20 % lot-selected FBS, 2 mM glutamine, 100 U/mL of penicillin, and 100 mg/mL of streptomycin sulfate (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Thioesterase superfamily member 1 undergoes stimulus-coupled conformational reorganization to regulate metabolism in mice
Article Snippet: The full-length cDNA for Them1 was cloned into a pVQAd CMV K-NpA vector (ViraQuest, North Liberty, IA) along with C-terminal EGFP downstream of the CMV promoter.

Expressing:

Article Title: Mechanism of dysfunction of human variants of the IRAK4 kinase and a role for its kinase activity in interleukin-1 receptor signaling
Article Snippet: IRAK4 constructs of the WT, D329A, and R12C compound variant containing C-terminal FLAG tag were cloned into pVQAd CMV K-NpA vector (Viraquest, North Liberty, IN).

Article Title: Genetic dissection of the miR-200–Zeb1 axis reveals its importance in tumor differentiation and invasion
Article Snippet: Fragments were cloned downstream of a CMV promoter in pVQAd CMV K-NpA for recombinant adenovirus production (ViraQuest).

Article Title: In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion
Article Snippet: Mouse Myc-DKK tagged cDNAs of Tial1 (NM_00009383) were provided by Origene and cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites KpnI and PmeI (Ad-Tial1).

Article Title: High density lipoprotein binding protein (Hdlbp/Vigilin) iRNA compositions and methods of use thereof
Article Snippet: Adenoviral Infections The sequence of V5-tagged hVigilin was cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites BamHI and XhoI (NEB).

Article Title: SorCS1 for the treatment of obesity
Article Snippet: TABLE 2 Sequence identity to human SorCS1 Protein DNA Species (% identity) (% identity) Human 100 100 Chimpanzee 99.6 99.4 Dog 97.6 92.5 Cow 92.9 89.8 Mouse 93.2 87.7 Rat 93.2 88.0 Chicken 85.3 79.7 FIGS. 17A-17B: Decreased plasma glucose levels in female wild-type and SorCS1 knockout mice after hepatic overexpression of soluble SorCS1.

Article Title: Inflammation-induced TRIM21 represses hepatic steatosis by promoting the ubiquitination of lipogenic regulators
Article Snippet: For TRIM21 overexpression, the human TRIM21 cDNA (NM_003141) tagged by Myc-DDK was provided by Origene (RC202088) and subcloned under the CMV promoter into the adenovirus expression vector pVQAd CMV K-NpA from Viraquest (Ad-Trim21).

Article Title: Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches
Article Snippet: hBMSC culture medium: α-MEM, 20 % lot-selected FBS, 2 mM glutamine, 100 U/mL of penicillin, and 100 mg/mL of streptomycin sulfate (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Thioesterase superfamily member 1 undergoes stimulus-coupled conformational reorganization to regulate metabolism in mice
Article Snippet: The full-length cDNA for Them1 was cloned into a pVQAd CMV K-NpA vector (ViraQuest, North Liberty, IA) along with C-terminal EGFP downstream of the CMV promoter.

Plasmid Preparation:

Article Title: Mechanism of dysfunction of human variants of the IRAK4 kinase and a role for its kinase activity in interleukin-1 receptor signaling
Article Snippet: IRAK4 constructs of the WT, D329A, and R12C compound variant containing C-terminal FLAG tag were cloned into pVQAd CMV K-NpA vector (Viraquest, North Liberty, IN).

Article Title: Genetic dissection of the miR-200–Zeb1 axis reveals its importance in tumor differentiation and invasion
Article Snippet: Fragments were cloned downstream of a CMV promoter in pVQAd CMV K-NpA for recombinant adenovirus production (ViraQuest).

Article Title: In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion
Article Snippet: Mouse Myc-DKK tagged cDNAs of Tial1 (NM_00009383) were provided by Origene and cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites KpnI and PmeI (Ad-Tial1).

Article Title: High density lipoprotein binding protein (Hdlbp/Vigilin) iRNA compositions and methods of use thereof
Article Snippet: Adenoviral Infections The sequence of V5-tagged hVigilin was cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites BamHI and XhoI (NEB).

Article Title: SorCS1 for the treatment of obesity
Article Snippet: TABLE 2 Sequence identity to human SorCS1 Protein DNA Species (% identity) (% identity) Human 100 100 Chimpanzee 99.6 99.4 Dog 97.6 92.5 Cow 92.9 89.8 Mouse 93.2 87.7 Rat 93.2 88.0 Chicken 85.3 79.7 FIGS. 17A-17B: Decreased plasma glucose levels in female wild-type and SorCS1 knockout mice after hepatic overexpression of soluble SorCS1.

Article Title: Inflammation-induced TRIM21 represses hepatic steatosis by promoting the ubiquitination of lipogenic regulators
Article Snippet: For TRIM21 overexpression, the human TRIM21 cDNA (NM_003141) tagged by Myc-DDK was provided by Origene (RC202088) and subcloned under the CMV promoter into the adenovirus expression vector pVQAd CMV K-NpA from Viraquest (Ad-Trim21).

Article Title: Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches
Article Snippet: hBMSC culture medium: α-MEM, 20 % lot-selected FBS, 2 mM glutamine, 100 U/mL of penicillin, and 100 mg/mL of streptomycin sulfate (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Thioesterase superfamily member 1 undergoes stimulus-coupled conformational reorganization to regulate metabolism in mice
Article Snippet: The full-length cDNA for Them1 was cloned into a pVQAd CMV K-NpA vector (ViraQuest, North Liberty, IA) along with C-terminal EGFP downstream of the CMV promoter.

Sequencing:

Article Title: Mechanism of dysfunction of human variants of the IRAK4 kinase and a role for its kinase activity in interleukin-1 receptor signaling
Article Snippet: IRAK4 constructs of the WT, D329A, and R12C compound variant containing C-terminal FLAG tag were cloned into pVQAd CMV K-NpA vector (Viraquest, North Liberty, IN).

Article Title: Genetic dissection of the miR-200–Zeb1 axis reveals its importance in tumor differentiation and invasion
Article Snippet: Fragments were cloned downstream of a CMV promoter in pVQAd CMV K-NpA for recombinant adenovirus production (ViraQuest).

Article Title: In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion
Article Snippet: Mouse Myc-DKK tagged cDNAs of Tial1 (NM_00009383) were provided by Origene and cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites KpnI and PmeI (Ad-Tial1).

Article Title: High density lipoprotein binding protein (Hdlbp/Vigilin) iRNA compositions and methods of use thereof
Article Snippet: Adenoviral Infections The sequence of V5-tagged hVigilin was cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites BamHI and XhoI (NEB).

Article Title: SorCS1 for the treatment of obesity
Article Snippet: TABLE 2 Sequence identity to human SorCS1 Protein DNA Species (% identity) (% identity) Human 100 100 Chimpanzee 99.6 99.4 Dog 97.6 92.5 Cow 92.9 89.8 Mouse 93.2 87.7 Rat 93.2 88.0 Chicken 85.3 79.7 FIGS. 17A-17B: Decreased plasma glucose levels in female wild-type and SorCS1 knockout mice after hepatic overexpression of soluble SorCS1.

Article Title: Inflammation-induced TRIM21 represses hepatic steatosis by promoting the ubiquitination of lipogenic regulators
Article Snippet: For TRIM21 overexpression, the human TRIM21 cDNA (NM_003141) tagged by Myc-DDK was provided by Origene (RC202088) and subcloned under the CMV promoter into the adenovirus expression vector pVQAd CMV K-NpA from Viraquest (Ad-Trim21).

Article Title: Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches
Article Snippet: hBMSC culture medium: α-MEM, 20 % lot-selected FBS, 2 mM glutamine, 100 U/mL of penicillin, and 100 mg/mL of streptomycin sulfate (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Thioesterase superfamily member 1 undergoes stimulus-coupled conformational reorganization to regulate metabolism in mice
Article Snippet: The full-length cDNA for Them1 was cloned into a pVQAd CMV K-NpA vector (ViraQuest, North Liberty, IA) along with C-terminal EGFP downstream of the CMV promoter.

Clone Assay:

Article Title: Mechanism of dysfunction of human variants of the IRAK4 kinase and a role for its kinase activity in interleukin-1 receptor signaling
Article Snippet: IRAK4 constructs of the WT, D329A, and R12C compound variant containing C-terminal FLAG tag were cloned into pVQAd CMV K-NpA vector (Viraquest, North Liberty, IN).

Article Title: Genetic dissection of the miR-200–Zeb1 axis reveals its importance in tumor differentiation and invasion
Article Snippet: Fragments were cloned downstream of a CMV promoter in pVQAd CMV K-NpA for recombinant adenovirus production (ViraQuest).

Article Title: In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion
Article Snippet: Mouse Myc-DKK tagged cDNAs of Tial1 (NM_00009383) were provided by Origene and cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites KpnI and PmeI (Ad-Tial1).

Article Title: High density lipoprotein binding protein (Hdlbp/Vigilin) iRNA compositions and methods of use thereof
Article Snippet: Adenoviral Infections The sequence of V5-tagged hVigilin was cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites BamHI and XhoI (NEB).

Article Title: SorCS1 for the treatment of obesity
Article Snippet: TABLE 2 Sequence identity to human SorCS1 Protein DNA Species (% identity) (% identity) Human 100 100 Chimpanzee 99.6 99.4 Dog 97.6 92.5 Cow 92.9 89.8 Mouse 93.2 87.7 Rat 93.2 88.0 Chicken 85.3 79.7 FIGS. 17A-17B: Decreased plasma glucose levels in female wild-type and SorCS1 knockout mice after hepatic overexpression of soluble SorCS1.

Article Title: Inflammation-induced TRIM21 represses hepatic steatosis by promoting the ubiquitination of lipogenic regulators
Article Snippet: For TRIM21 overexpression, the human TRIM21 cDNA (NM_003141) tagged by Myc-DDK was provided by Origene (RC202088) and subcloned under the CMV promoter into the adenovirus expression vector pVQAd CMV K-NpA from Viraquest (Ad-Trim21).

Article Title: Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches
Article Snippet: hBMSC culture medium: α-MEM, 20 % lot-selected FBS, 2 mM glutamine, 100 U/mL of penicillin, and 100 mg/mL of streptomycin sulfate (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Thioesterase superfamily member 1 undergoes stimulus-coupled conformational reorganization to regulate metabolism in mice
Article Snippet: The full-length cDNA for Them1 was cloned into a pVQAd CMV K-NpA vector (ViraQuest, North Liberty, IA) along with C-terminal EGFP downstream of the CMV promoter.

Virus:

Article Title: Mechanism of dysfunction of human variants of the IRAK4 kinase and a role for its kinase activity in interleukin-1 receptor signaling
Article Snippet: IRAK4 constructs of the WT, D329A, and R12C compound variant containing C-terminal FLAG tag were cloned into pVQAd CMV K-NpA vector (Viraquest, North Liberty, IN).

Article Title: Genetic dissection of the miR-200–Zeb1 axis reveals its importance in tumor differentiation and invasion
Article Snippet: Fragments were cloned downstream of a CMV promoter in pVQAd CMV K-NpA for recombinant adenovirus production (ViraQuest).

Article Title: In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion
Article Snippet: Mouse Myc-DKK tagged cDNAs of Tial1 (NM_00009383) were provided by Origene and cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites KpnI and PmeI (Ad-Tial1).

Article Title: High density lipoprotein binding protein (Hdlbp/Vigilin) iRNA compositions and methods of use thereof
Article Snippet: Adenoviral Infections The sequence of V5-tagged hVigilin was cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites BamHI and XhoI (NEB).

Article Title: SorCS1 for the treatment of obesity
Article Snippet: TABLE 2 Sequence identity to human SorCS1 Protein DNA Species (% identity) (% identity) Human 100 100 Chimpanzee 99.6 99.4 Dog 97.6 92.5 Cow 92.9 89.8 Mouse 93.2 87.7 Rat 93.2 88.0 Chicken 85.3 79.7 FIGS. 17A-17B: Decreased plasma glucose levels in female wild-type and SorCS1 knockout mice after hepatic overexpression of soluble SorCS1.

Article Title: Inflammation-induced TRIM21 represses hepatic steatosis by promoting the ubiquitination of lipogenic regulators
Article Snippet: For TRIM21 overexpression, the human TRIM21 cDNA (NM_003141) tagged by Myc-DDK was provided by Origene (RC202088) and subcloned under the CMV promoter into the adenovirus expression vector pVQAd CMV K-NpA from Viraquest (Ad-Trim21).

Article Title: Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches
Article Snippet: hBMSC culture medium: α-MEM, 20 % lot-selected FBS, 2 mM glutamine, 100 U/mL of penicillin, and 100 mg/mL of streptomycin sulfate (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Thioesterase superfamily member 1 undergoes stimulus-coupled conformational reorganization to regulate metabolism in mice
Article Snippet: The full-length cDNA for Them1 was cloned into a pVQAd CMV K-NpA vector (ViraQuest, North Liberty, IA) along with C-terminal EGFP downstream of the CMV promoter.

Negative Control:

Article Title: Mechanism of dysfunction of human variants of the IRAK4 kinase and a role for its kinase activity in interleukin-1 receptor signaling
Article Snippet: IRAK4 constructs of the WT, D329A, and R12C compound variant containing C-terminal FLAG tag were cloned into pVQAd CMV K-NpA vector (Viraquest, North Liberty, IN).

Article Title: Genetic dissection of the miR-200–Zeb1 axis reveals its importance in tumor differentiation and invasion
Article Snippet: Fragments were cloned downstream of a CMV promoter in pVQAd CMV K-NpA for recombinant adenovirus production (ViraQuest).

Article Title: In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion
Article Snippet: Mouse Myc-DKK tagged cDNAs of Tial1 (NM_00009383) were provided by Origene and cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites KpnI and PmeI (Ad-Tial1).

Article Title: High density lipoprotein binding protein (Hdlbp/Vigilin) iRNA compositions and methods of use thereof
Article Snippet: Adenoviral Infections The sequence of V5-tagged hVigilin was cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites BamHI and XhoI (NEB).

Article Title: SorCS1 for the treatment of obesity
Article Snippet: TABLE 2 Sequence identity to human SorCS1 Protein DNA Species (% identity) (% identity) Human 100 100 Chimpanzee 99.6 99.4 Dog 97.6 92.5 Cow 92.9 89.8 Mouse 93.2 87.7 Rat 93.2 88.0 Chicken 85.3 79.7 FIGS. 17A-17B: Decreased plasma glucose levels in female wild-type and SorCS1 knockout mice after hepatic overexpression of soluble SorCS1.

Article Title: Inflammation-induced TRIM21 represses hepatic steatosis by promoting the ubiquitination of lipogenic regulators
Article Snippet: For TRIM21 overexpression, the human TRIM21 cDNA (NM_003141) tagged by Myc-DDK was provided by Origene (RC202088) and subcloned under the CMV promoter into the adenovirus expression vector pVQAd CMV K-NpA from Viraquest (Ad-Trim21).

Article Title: Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches
Article Snippet: hBMSC culture medium: α-MEM, 20 % lot-selected FBS, 2 mM glutamine, 100 U/mL of penicillin, and 100 mg/mL of streptomycin sulfate (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Thioesterase superfamily member 1 undergoes stimulus-coupled conformational reorganization to regulate metabolism in mice
Article Snippet: The full-length cDNA for Them1 was cloned into a pVQAd CMV K-NpA vector (ViraQuest, North Liberty, IA) along with C-terminal EGFP downstream of the CMV promoter.

Injection:

Article Title: Mechanism of dysfunction of human variants of the IRAK4 kinase and a role for its kinase activity in interleukin-1 receptor signaling
Article Snippet: IRAK4 constructs of the WT, D329A, and R12C compound variant containing C-terminal FLAG tag were cloned into pVQAd CMV K-NpA vector (Viraquest, North Liberty, IN).

Article Title: Genetic dissection of the miR-200–Zeb1 axis reveals its importance in tumor differentiation and invasion
Article Snippet: Fragments were cloned downstream of a CMV promoter in pVQAd CMV K-NpA for recombinant adenovirus production (ViraQuest).

Article Title: In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion
Article Snippet: Mouse Myc-DKK tagged cDNAs of Tial1 (NM_00009383) were provided by Origene and cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites KpnI and PmeI (Ad-Tial1).

Article Title: High density lipoprotein binding protein (Hdlbp/Vigilin) iRNA compositions and methods of use thereof
Article Snippet: Adenoviral Infections The sequence of V5-tagged hVigilin was cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites BamHI and XhoI (NEB).

Article Title: SorCS1 for the treatment of obesity
Article Snippet: TABLE 2 Sequence identity to human SorCS1 Protein DNA Species (% identity) (% identity) Human 100 100 Chimpanzee 99.6 99.4 Dog 97.6 92.5 Cow 92.9 89.8 Mouse 93.2 87.7 Rat 93.2 88.0 Chicken 85.3 79.7 FIGS. 17A-17B: Decreased plasma glucose levels in female wild-type and SorCS1 knockout mice after hepatic overexpression of soluble SorCS1.

Article Title: Inflammation-induced TRIM21 represses hepatic steatosis by promoting the ubiquitination of lipogenic regulators
Article Snippet: For TRIM21 overexpression, the human TRIM21 cDNA (NM_003141) tagged by Myc-DDK was provided by Origene (RC202088) and subcloned under the CMV promoter into the adenovirus expression vector pVQAd CMV K-NpA from Viraquest (Ad-Trim21).

Article Title: Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches
Article Snippet: hBMSC culture medium: α-MEM, 20 % lot-selected FBS, 2 mM glutamine, 100 U/mL of penicillin, and 100 mg/mL of streptomycin sulfate (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Thioesterase superfamily member 1 undergoes stimulus-coupled conformational reorganization to regulate metabolism in mice
Article Snippet: The full-length cDNA for Them1 was cloned into a pVQAd CMV K-NpA vector (ViraQuest, North Liberty, IA) along with C-terminal EGFP downstream of the CMV promoter.

Recombinant:

Article Title: Mechanism of dysfunction of human variants of the IRAK4 kinase and a role for its kinase activity in interleukin-1 receptor signaling
Article Snippet: IRAK4 constructs of the WT, D329A, and R12C compound variant containing C-terminal FLAG tag were cloned into pVQAd CMV K-NpA vector (Viraquest, North Liberty, IN).

Article Title: Genetic dissection of the miR-200–Zeb1 axis reveals its importance in tumor differentiation and invasion
Article Snippet: Fragments were cloned downstream of a CMV promoter in pVQAd CMV K-NpA for recombinant adenovirus production (ViraQuest).

Article Title: In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion
Article Snippet: Mouse Myc-DKK tagged cDNAs of Tial1 (NM_00009383) were provided by Origene and cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites KpnI and PmeI (Ad-Tial1).

Article Title: High density lipoprotein binding protein (Hdlbp/Vigilin) iRNA compositions and methods of use thereof
Article Snippet: Adenoviral Infections The sequence of V5-tagged hVigilin was cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites BamHI and XhoI (NEB).

Article Title: SorCS1 for the treatment of obesity
Article Snippet: TABLE 2 Sequence identity to human SorCS1 Protein DNA Species (% identity) (% identity) Human 100 100 Chimpanzee 99.6 99.4 Dog 97.6 92.5 Cow 92.9 89.8 Mouse 93.2 87.7 Rat 93.2 88.0 Chicken 85.3 79.7 FIGS. 17A-17B: Decreased plasma glucose levels in female wild-type and SorCS1 knockout mice after hepatic overexpression of soluble SorCS1.

Article Title: Inflammation-induced TRIM21 represses hepatic steatosis by promoting the ubiquitination of lipogenic regulators
Article Snippet: For TRIM21 overexpression, the human TRIM21 cDNA (NM_003141) tagged by Myc-DDK was provided by Origene (RC202088) and subcloned under the CMV promoter into the adenovirus expression vector pVQAd CMV K-NpA from Viraquest (Ad-Trim21).

Article Title: Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches
Article Snippet: hBMSC culture medium: α-MEM, 20 % lot-selected FBS, 2 mM glutamine, 100 U/mL of penicillin, and 100 mg/mL of streptomycin sulfate (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Thioesterase superfamily member 1 undergoes stimulus-coupled conformational reorganization to regulate metabolism in mice
Article Snippet: The full-length cDNA for Them1 was cloned into a pVQAd CMV K-NpA vector (ViraQuest, North Liberty, IA) along with C-terminal EGFP downstream of the CMV promoter.

Construct:

Article Title: Mechanism of dysfunction of human variants of the IRAK4 kinase and a role for its kinase activity in interleukin-1 receptor signaling
Article Snippet: IRAK4 constructs of the WT, D329A, and R12C compound variant containing C-terminal FLAG tag were cloned into pVQAd CMV K-NpA vector (Viraquest, North Liberty, IN).

Article Title: Genetic dissection of the miR-200–Zeb1 axis reveals its importance in tumor differentiation and invasion
Article Snippet: Fragments were cloned downstream of a CMV promoter in pVQAd CMV K-NpA for recombinant adenovirus production (ViraQuest).

Article Title: In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion
Article Snippet: Mouse Myc-DKK tagged cDNAs of Tial1 (NM_00009383) were provided by Origene and cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites KpnI and PmeI (Ad-Tial1).

Article Title: High density lipoprotein binding protein (Hdlbp/Vigilin) iRNA compositions and methods of use thereof
Article Snippet: Adenoviral Infections The sequence of V5-tagged hVigilin was cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites BamHI and XhoI (NEB).

Article Title: SorCS1 for the treatment of obesity
Article Snippet: TABLE 2 Sequence identity to human SorCS1 Protein DNA Species (% identity) (% identity) Human 100 100 Chimpanzee 99.6 99.4 Dog 97.6 92.5 Cow 92.9 89.8 Mouse 93.2 87.7 Rat 93.2 88.0 Chicken 85.3 79.7 FIGS. 17A-17B: Decreased plasma glucose levels in female wild-type and SorCS1 knockout mice after hepatic overexpression of soluble SorCS1.

Article Title: Inflammation-induced TRIM21 represses hepatic steatosis by promoting the ubiquitination of lipogenic regulators
Article Snippet: For TRIM21 overexpression, the human TRIM21 cDNA (NM_003141) tagged by Myc-DDK was provided by Origene (RC202088) and subcloned under the CMV promoter into the adenovirus expression vector pVQAd CMV K-NpA from Viraquest (Ad-Trim21).

Article Title: Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches
Article Snippet: hBMSC culture medium: α-MEM, 20 % lot-selected FBS, 2 mM glutamine, 100 U/mL of penicillin, and 100 mg/mL of streptomycin sulfate (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Thioesterase superfamily member 1 undergoes stimulus-coupled conformational reorganization to regulate metabolism in mice
Article Snippet: The full-length cDNA for Them1 was cloned into a pVQAd CMV K-NpA vector (ViraQuest, North Liberty, IA) along with C-terminal EGFP downstream of the CMV promoter.

Variant Assay:

Article Title: Mechanism of dysfunction of human variants of the IRAK4 kinase and a role for its kinase activity in interleukin-1 receptor signaling
Article Snippet: IRAK4 constructs of the WT, D329A, and R12C compound variant containing C-terminal FLAG tag were cloned into pVQAd CMV K-NpA vector (Viraquest, North Liberty, IN).

Article Title: Genetic dissection of the miR-200–Zeb1 axis reveals its importance in tumor differentiation and invasion
Article Snippet: Fragments were cloned downstream of a CMV promoter in pVQAd CMV K-NpA for recombinant adenovirus production (ViraQuest).

Article Title: In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion
Article Snippet: Mouse Myc-DKK tagged cDNAs of Tial1 (NM_00009383) were provided by Origene and cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites KpnI and PmeI (Ad-Tial1).

Article Title: High density lipoprotein binding protein (Hdlbp/Vigilin) iRNA compositions and methods of use thereof
Article Snippet: Adenoviral Infections The sequence of V5-tagged hVigilin was cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites BamHI and XhoI (NEB).

Article Title: SorCS1 for the treatment of obesity
Article Snippet: TABLE 2 Sequence identity to human SorCS1 Protein DNA Species (% identity) (% identity) Human 100 100 Chimpanzee 99.6 99.4 Dog 97.6 92.5 Cow 92.9 89.8 Mouse 93.2 87.7 Rat 93.2 88.0 Chicken 85.3 79.7 FIGS. 17A-17B: Decreased plasma glucose levels in female wild-type and SorCS1 knockout mice after hepatic overexpression of soluble SorCS1.

Article Title: Inflammation-induced TRIM21 represses hepatic steatosis by promoting the ubiquitination of lipogenic regulators
Article Snippet: For TRIM21 overexpression, the human TRIM21 cDNA (NM_003141) tagged by Myc-DDK was provided by Origene (RC202088) and subcloned under the CMV promoter into the adenovirus expression vector pVQAd CMV K-NpA from Viraquest (Ad-Trim21).

Article Title: Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches
Article Snippet: hBMSC culture medium: α-MEM, 20 % lot-selected FBS, 2 mM glutamine, 100 U/mL of penicillin, and 100 mg/mL of streptomycin sulfate (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Thioesterase superfamily member 1 undergoes stimulus-coupled conformational reorganization to regulate metabolism in mice
Article Snippet: The full-length cDNA for Them1 was cloned into a pVQAd CMV K-NpA vector (ViraQuest, North Liberty, IA) along with C-terminal EGFP downstream of the CMV promoter.

FLAG-tag:

Article Title: Mechanism of dysfunction of human variants of the IRAK4 kinase and a role for its kinase activity in interleukin-1 receptor signaling
Article Snippet: IRAK4 constructs of the WT, D329A, and R12C compound variant containing C-terminal FLAG tag were cloned into pVQAd CMV K-NpA vector (Viraquest, North Liberty, IN).

Article Title: Genetic dissection of the miR-200–Zeb1 axis reveals its importance in tumor differentiation and invasion
Article Snippet: Fragments were cloned downstream of a CMV promoter in pVQAd CMV K-NpA for recombinant adenovirus production (ViraQuest).

Article Title: In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion
Article Snippet: Mouse Myc-DKK tagged cDNAs of Tial1 (NM_00009383) were provided by Origene and cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites KpnI and PmeI (Ad-Tial1).

Article Title: High density lipoprotein binding protein (Hdlbp/Vigilin) iRNA compositions and methods of use thereof
Article Snippet: Adenoviral Infections The sequence of V5-tagged hVigilin was cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites BamHI and XhoI (NEB).

Article Title: SorCS1 for the treatment of obesity
Article Snippet: TABLE 2 Sequence identity to human SorCS1 Protein DNA Species (% identity) (% identity) Human 100 100 Chimpanzee 99.6 99.4 Dog 97.6 92.5 Cow 92.9 89.8 Mouse 93.2 87.7 Rat 93.2 88.0 Chicken 85.3 79.7 FIGS. 17A-17B: Decreased plasma glucose levels in female wild-type and SorCS1 knockout mice after hepatic overexpression of soluble SorCS1.

Article Title: Inflammation-induced TRIM21 represses hepatic steatosis by promoting the ubiquitination of lipogenic regulators
Article Snippet: For TRIM21 overexpression, the human TRIM21 cDNA (NM_003141) tagged by Myc-DDK was provided by Origene (RC202088) and subcloned under the CMV promoter into the adenovirus expression vector pVQAd CMV K-NpA from Viraquest (Ad-Trim21).

Article Title: Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches
Article Snippet: hBMSC culture medium: α-MEM, 20 % lot-selected FBS, 2 mM glutamine, 100 U/mL of penicillin, and 100 mg/mL of streptomycin sulfate (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Thioesterase superfamily member 1 undergoes stimulus-coupled conformational reorganization to regulate metabolism in mice
Article Snippet: The full-length cDNA for Them1 was cloned into a pVQAd CMV K-NpA vector (ViraQuest, North Liberty, IA) along with C-terminal EGFP downstream of the CMV promoter.

Infection:

Article Title: Mechanism of dysfunction of human variants of the IRAK4 kinase and a role for its kinase activity in interleukin-1 receptor signaling
Article Snippet: IRAK4 constructs of the WT, D329A, and R12C compound variant containing C-terminal FLAG tag were cloned into pVQAd CMV K-NpA vector (Viraquest, North Liberty, IN).

Article Title: Genetic dissection of the miR-200–Zeb1 axis reveals its importance in tumor differentiation and invasion
Article Snippet: Fragments were cloned downstream of a CMV promoter in pVQAd CMV K-NpA for recombinant adenovirus production (ViraQuest).

Article Title: In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion
Article Snippet: Mouse Myc-DKK tagged cDNAs of Tial1 (NM_00009383) were provided by Origene and cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites KpnI and PmeI (Ad-Tial1).

Article Title: High density lipoprotein binding protein (Hdlbp/Vigilin) iRNA compositions and methods of use thereof
Article Snippet: Adenoviral Infections The sequence of V5-tagged hVigilin was cloned into pVQAd CMV K-NpA (Viraquest) using the restriction sites BamHI and XhoI (NEB).

Article Title: SorCS1 for the treatment of obesity
Article Snippet: TABLE 2 Sequence identity to human SorCS1 Protein DNA Species (% identity) (% identity) Human 100 100 Chimpanzee 99.6 99.4 Dog 97.6 92.5 Cow 92.9 89.8 Mouse 93.2 87.7 Rat 93.2 88.0 Chicken 85.3 79.7 FIGS. 17A-17B: Decreased plasma glucose levels in female wild-type and SorCS1 knockout mice after hepatic overexpression of soluble SorCS1.

Article Title: Inflammation-induced TRIM21 represses hepatic steatosis by promoting the ubiquitination of lipogenic regulators
Article Snippet: For TRIM21 overexpression, the human TRIM21 cDNA (NM_003141) tagged by Myc-DDK was provided by Origene (RC202088) and subcloned under the CMV promoter into the adenovirus expression vector pVQAd CMV K-NpA from Viraquest (Ad-Trim21).

Article Title: Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches
Article Snippet: hBMSC culture medium: α-MEM, 20 % lot-selected FBS, 2 mM glutamine, 100 U/mL of penicillin, and 100 mg/mL of streptomycin sulfate (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Thioesterase superfamily member 1 undergoes stimulus-coupled conformational reorganization to regulate metabolism in mice
Article Snippet: The full-length cDNA for Them1 was cloned into a pVQAd CMV K-NpA vector (ViraQuest, North Liberty, IA) along with C-terminal EGFP downstream of the CMV promoter.



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