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human heart tissue cryosections  (OriGene)


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    Structured Review

    OriGene human heart tissue cryosections
    Fig. 4. The t-tubule network develops in the absence of O-mannosylated α-DG. (A and B) Immunofluorescence of <t>cryosections</t> of ventricles from control and Pomt1 cKO mice to detect matriglycan (A and B) and β-DG (B). Ten µm cryosections were used in A. (Scale bar, 10 µm.) Sixteen µm cryosections were used in B. (Scale bar, 5 µm.) (C) FM 464-FX fluorescence of whole left ventricles to detect plasma membranes, including t-tubule membranes. (Scale bar, 20 µm.) (D) Quantification of the percent of myofibers with normal or disrupted t-tubule staining within a 90× magnification field. (E) Line scan analysis to determine the peak signal intensity of FM 464-FX labeled t-tubules (TT). (F) Number of t-tubules observed within 16 µm regions. Image analysis was performed on hearts of mice from both sexes (n = 4 controls; n = 5 cKO). Unpaired t tests with the Holm–Sidak post hoc test were performed. Data are expressed as mean ± SD.
    Human Heart Tissue Cryosections, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tissue+sections/Frozen+Tissue+Sections%2C+Heart/pm38771868-337-0-7
    Average 92 stars, based on 1 article reviews
    human heart tissue cryosections - by Bioz Stars, 2026-11
    92/100 stars

    Images

    1) Product Images from "Matriglycan maintains t-tubule structural integrity in cardiac muscle."

    Article Title: Matriglycan maintains t-tubule structural integrity in cardiac muscle.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.2402890121

    Fig. 4. The t-tubule network develops in the absence of O-mannosylated α-DG. (A and B) Immunofluorescence of cryosections of ventricles from control and Pomt1 cKO mice to detect matriglycan (A and B) and β-DG (B). Ten µm cryosections were used in A. (Scale bar, 10 µm.) Sixteen µm cryosections were used in B. (Scale bar, 5 µm.) (C) FM 464-FX fluorescence of whole left ventricles to detect plasma membranes, including t-tubule membranes. (Scale bar, 20 µm.) (D) Quantification of the percent of myofibers with normal or disrupted t-tubule staining within a 90× magnification field. (E) Line scan analysis to determine the peak signal intensity of FM 464-FX labeled t-tubules (TT). (F) Number of t-tubules observed within 16 µm regions. Image analysis was performed on hearts of mice from both sexes (n = 4 controls; n = 5 cKO). Unpaired t tests with the Holm–Sidak post hoc test were performed. Data are expressed as mean ± SD.
    Figure Legend Snippet: Fig. 4. The t-tubule network develops in the absence of O-mannosylated α-DG. (A and B) Immunofluorescence of cryosections of ventricles from control and Pomt1 cKO mice to detect matriglycan (A and B) and β-DG (B). Ten µm cryosections were used in A. (Scale bar, 10 µm.) Sixteen µm cryosections were used in B. (Scale bar, 5 µm.) (C) FM 464-FX fluorescence of whole left ventricles to detect plasma membranes, including t-tubule membranes. (Scale bar, 20 µm.) (D) Quantification of the percent of myofibers with normal or disrupted t-tubule staining within a 90× magnification field. (E) Line scan analysis to determine the peak signal intensity of FM 464-FX labeled t-tubules (TT). (F) Number of t-tubules observed within 16 µm regions. Image analysis was performed on hearts of mice from both sexes (n = 4 controls; n = 5 cKO). Unpaired t tests with the Holm–Sidak post hoc test were performed. Data are expressed as mean ± SD.

    Techniques Used: Immunofluorescence, Control, Fluorescence, Clinical Proteomics, Staining, Labeling

    Related Articles

    Immunofluorescence:

    Article Title: Matriglycan maintains t-tubule structural integrity in cardiac muscle.
    Article Snippet: Peak intensity and t- tubule count were averaged across the two regions of interest per myofiber.Peak intensity and t- tubule count were averaged across the two regions of interest per myofiber.. Human heart tissue cryosections were purchased from OriGene (CS616190; CS616185; Rockville, Maryland, USA).. Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.

    Article Title: Matriglycan maintains t-tubule structural integrity in cardiac muscle
    Article Snippet: Peak intensity and t-tubule count were averaged across the two regions of interest per myofiber.Peak intensity and t-tubule count were averaged across the two regions of interest per myofiber.. Human heart tissue cryosections were purchased from OriGene (CS616190; CS616185; Rockville, Maryland, USA).. Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.

    Control:

    Article Title: Matriglycan maintains t-tubule structural integrity in cardiac muscle.
    Article Snippet: Peak intensity and t- tubule count were averaged across the two regions of interest per myofiber.Peak intensity and t- tubule count were averaged across the two regions of interest per myofiber.. Human heart tissue cryosections were purchased from OriGene (CS616190; CS616185; Rockville, Maryland, USA).. Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.

    Article Title: Matriglycan maintains t-tubule structural integrity in cardiac muscle
    Article Snippet: Peak intensity and t-tubule count were averaged across the two regions of interest per myofiber.Peak intensity and t-tubule count were averaged across the two regions of interest per myofiber.. Human heart tissue cryosections were purchased from OriGene (CS616190; CS616185; Rockville, Maryland, USA).. Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.

    Fluorescence:

    Article Title: Matriglycan maintains t-tubule structural integrity in cardiac muscle.
    Article Snippet: Peak intensity and t- tubule count were averaged across the two regions of interest per myofiber.Peak intensity and t- tubule count were averaged across the two regions of interest per myofiber.. Human heart tissue cryosections were purchased from OriGene (CS616190; CS616185; Rockville, Maryland, USA).. Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.

    Article Title: Matriglycan maintains t-tubule structural integrity in cardiac muscle
    Article Snippet: Peak intensity and t-tubule count were averaged across the two regions of interest per myofiber.Peak intensity and t-tubule count were averaged across the two regions of interest per myofiber.. Human heart tissue cryosections were purchased from OriGene (CS616190; CS616185; Rockville, Maryland, USA).. Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.

    Clinical Proteomics:

    Article Title: Matriglycan maintains t-tubule structural integrity in cardiac muscle.
    Article Snippet: Peak intensity and t- tubule count were averaged across the two regions of interest per myofiber.Peak intensity and t- tubule count were averaged across the two regions of interest per myofiber.. Human heart tissue cryosections were purchased from OriGene (CS616190; CS616185; Rockville, Maryland, USA).. Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.

    Article Title: Matriglycan maintains t-tubule structural integrity in cardiac muscle
    Article Snippet: Peak intensity and t-tubule count were averaged across the two regions of interest per myofiber.Peak intensity and t-tubule count were averaged across the two regions of interest per myofiber.. Human heart tissue cryosections were purchased from OriGene (CS616190; CS616185; Rockville, Maryland, USA).. Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.

    Staining:

    Article Title: Matriglycan maintains t-tubule structural integrity in cardiac muscle.
    Article Snippet: Peak intensity and t- tubule count were averaged across the two regions of interest per myofiber.Peak intensity and t- tubule count were averaged across the two regions of interest per myofiber.. Human heart tissue cryosections were purchased from OriGene (CS616190; CS616185; Rockville, Maryland, USA).. Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.

    Article Title: Matriglycan maintains t-tubule structural integrity in cardiac muscle
    Article Snippet: Peak intensity and t-tubule count were averaged across the two regions of interest per myofiber.Peak intensity and t-tubule count were averaged across the two regions of interest per myofiber.. Human heart tissue cryosections were purchased from OriGene (CS616190; CS616185; Rockville, Maryland, USA).. Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.

    Labeling:

    Article Title: Matriglycan maintains t-tubule structural integrity in cardiac muscle.
    Article Snippet: Peak intensity and t- tubule count were averaged across the two regions of interest per myofiber.Peak intensity and t- tubule count were averaged across the two regions of interest per myofiber.. Human heart tissue cryosections were purchased from OriGene (CS616190; CS616185; Rockville, Maryland, USA).. Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.

    Article Title: Matriglycan maintains t-tubule structural integrity in cardiac muscle
    Article Snippet: Peak intensity and t-tubule count were averaged across the two regions of interest per myofiber.Peak intensity and t-tubule count were averaged across the two regions of interest per myofiber.. Human heart tissue cryosections were purchased from OriGene (CS616190; CS616185; Rockville, Maryland, USA).. Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.Five μm frozen tissue sections were mounted onto a glass slide and shipped on dry ice to the University of Iowa.



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