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multiple tissue northern blot  (OriGene)


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    Structured Review

    OriGene multiple tissue northern blot
    Figure 1. Expression of a gene identified by differential display. (A) <t>Northern</t> <t>blot</t> showing differential expression of an RNA species in Fas-sensitive primary B cells stimu- lated by CD40L alone (2) and in Fas-resistant B cells stimulated by CD40L plus anti-Ig (1), as de- scribed in Materials and Methods. Arrows indicate the location of ri- bosomal RNA. (B) Northern blot showing expression of an RNA species in various murine <t>tissues,</t> as indicated in figure.
    Multiple Tissue Northern Blot, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tissue+sections/Frozen+Tissue+Sections%2C+Multiple+digestive+system+sites/pm10075978-50-48-55
    Average 90 stars, based on 8 article reviews
    multiple tissue northern blot - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "A novel gene coding for a Fas apoptosis inhibitory molecule (FAIM) isolated from inducibly Fas-resistant B lymphocytes."

    Article Title: A novel gene coding for a Fas apoptosis inhibitory molecule (FAIM) isolated from inducibly Fas-resistant B lymphocytes.

    Journal: The Journal of experimental medicine

    doi: 10.1084/jem.189.6.949

    Figure 1. Expression of a gene identified by differential display. (A) Northern blot showing differential expression of an RNA species in Fas-sensitive primary B cells stimu- lated by CD40L alone (2) and in Fas-resistant B cells stimulated by CD40L plus anti-Ig (1), as de- scribed in Materials and Methods. Arrows indicate the location of ri- bosomal RNA. (B) Northern blot showing expression of an RNA species in various murine tissues, as indicated in figure.
    Figure Legend Snippet: Figure 1. Expression of a gene identified by differential display. (A) Northern blot showing differential expression of an RNA species in Fas-sensitive primary B cells stimu- lated by CD40L alone (2) and in Fas-resistant B cells stimulated by CD40L plus anti-Ig (1), as de- scribed in Materials and Methods. Arrows indicate the location of ri- bosomal RNA. (B) Northern blot showing expression of an RNA species in various murine tissues, as indicated in figure.

    Techniques Used: Expressing, Northern Blot, Quantitative Proteomics

    Related Articles

    Hybridization:

    Article Title: Structure, function, and tissue expression pattern of human SN2, a subtype of the amino acid transport system N.
    Article Snippet: w 9 a c n a m i w i o l e t c We have cloned a new subtype of the amino acid ransport system N from a human liver cell line.. This ransporter, designated SN2, consists of 472 amino acds and exhibits 62% identity with human SN1 at the evel of amino acid sequence.. SN2-specific transcripts re expressed predominantly in the stomach, brain, iver, lung, and intestinal tract.

    Article Title: TNAP, a Novel Repressor of NF-κB-inducing Kinase, Suppresses NF-κB Activation
    Article Snippet: .. The tissue-specific expression of TNAP was examined by hybridization of a human multiple tissue Northern blot containing 2 g of poly(A) RNA (OriGene). ..

    Expressing:

    Article Title: Structure, function, and tissue expression pattern of human SN2, a subtype of the amino acid transport system N.
    Article Snippet: w 9 a c n a m i w i o l e t c We have cloned a new subtype of the amino acid ransport system N from a human liver cell line.. This ransporter, designated SN2, consists of 472 amino acds and exhibits 62% identity with human SN1 at the evel of amino acid sequence.. SN2-specific transcripts re expressed predominantly in the stomach, brain, iver, lung, and intestinal tract.

    Article Title: TNAP, a Novel Repressor of NF-κB-inducing Kinase, Suppresses NF-κB Activation
    Article Snippet: .. The tissue-specific expression of TNAP was examined by hybridization of a human multiple tissue Northern blot containing 2 g of poly(A) RNA (OriGene). ..

    Northern Blot:

    Article Title: The human and mouse methylenetetrahydrofolate reductase (MTHFR) genes: genomic organization, mRNA structure and linkage to the CLCN6 gene.
    Article Snippet: Methylenetetrahydrofolate reductase (MTHFR), a pivotal enzyme in folate metabolism, regulates the proportional distribution of one-carbon moieties between cellular methylation reactions and nucleic acid synthesis.. The organization of the MTHFR gene and the structure of its mRNA were characterized in human and mouse.. There are three mRNA transcripts of 2.8, 7.2 and 9.8 kb in human and two of 3.2 and 7.5 kb in mouse.

    Article Title: Siglec-9, a Novel Sialic Acid Binding Member of the Immunoglobulin Superfamily Expressed Broadly on Human Blood Leukocytes
    Article Snippet: .. Northern Blot Analysis—A human Multiple Tissue Northern blot containing approximately 2 mg of poly(A)1 RNA per lane from various human tissues was purchased from Origene Technologies Ltd. (Rockville, MD) and hybridized with a 32P-labeled probe corresponding to a region of Siglec-9 (bases 960–1522) that was predicted not to crosshybridize with mRNA encoding the other highly related Siglecs. ..

    Article Title: Characterization of KIBRA, a novel WW domain-containing protein.
    Article Snippet: In a yeast two hybrid screen with the human isoform of Dendrin (KIAA0749), a putative modulator of the postsynaptic cytoskeleton, we isolated a cDNA coding for a novel protein, KIBRA, possessing two amino-terminal WW domains, an internal C2like domain and a carboxy-terminal glutamic acid-rich stretch.. Northern blot analysis revealed that the expression of KIBRA mRNA was predominately found in kidney and brain.. In vitro interaction studies revealed that the first KIBRA WW domain binds specifically to PPxY motifs.

    Article Title: Interaction between Tyrosine Kinase Etk and a RUN Domain- and FYVE Domain-containing Protein RUFY1
    Article Snippet: The bound primary antibody was visualized by enhanced chemiluminescent detection (Amersham Biosciences). .. The human multiple tissue blots were purchased from OriGene Technologies, Inc. Northern blotting was performed as described in the manufacturer’s application guide using 32P-labeled cDNA probes specific for human RUFY1, RUFY2, and -actin (control), respectively. ..

    Article Title: Molecular and Functional Analysis of a Novel Neuronal Vesicular Glutamate Transporter
    Article Snippet: .. Northern Blot Analysis—A mouse, multiple-tissue Northern blot was purchased from Origene (Rockville, MD). ..

    Article Title: TNAP, a Novel Repressor of NF-κB-inducing Kinase, Suppresses NF-κB Activation
    Article Snippet: .. The tissue-specific expression of TNAP was examined by hybridization of a human multiple tissue Northern blot containing 2 g of poly(A) RNA (OriGene). ..

    Nick Translation:

    Article Title: Characterization of KIBRA, a novel WW domain-containing protein.
    Article Snippet: In a yeast two hybrid screen with the human isoform of Dendrin (KIAA0749), a putative modulator of the postsynaptic cytoskeleton, we isolated a cDNA coding for a novel protein, KIBRA, possessing two amino-terminal WW domains, an internal C2like domain and a carboxy-terminal glutamic acid-rich stretch.. Northern blot analysis revealed that the expression of KIBRA mRNA was predominately found in kidney and brain.. In vitro interaction studies revealed that the first KIBRA WW domain binds specifically to PPxY motifs.

    Control:

    Article Title: Interaction between Tyrosine Kinase Etk and a RUN Domain- and FYVE Domain-containing Protein RUFY1
    Article Snippet: The bound primary antibody was visualized by enhanced chemiluminescent detection (Amersham Biosciences). .. The human multiple tissue blots were purchased from OriGene Technologies, Inc. Northern blotting was performed as described in the manufacturer’s application guide using 32P-labeled cDNA probes specific for human RUFY1, RUFY2, and -actin (control), respectively. ..



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