ki67 (Danaher Inc)
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99
Structured Review
Danaher Inc
ki67
Ki67, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 10855 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue+microarray+based+immunohistochemistry+analysis/Anti-Ki67+antibody/pmc08487498-215-44-47
Average 99 stars, based on 10855 article reviews
Ki67, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 10855 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue+microarray+based+immunohistochemistry+analysis/Anti-Ki67+antibody/pmc08487498-215-44-47
Average 99 stars, based on 10855 article reviews
ki67 - by Bioz Stars,
2026-11
99/100 stars
Images
1) Product Images from "Distinct B cell subsets in Peyer’s patches convey probiotic effects by Limosilactobacillus reuteri"
Article Title: Distinct B cell subsets in Peyer’s patches convey probiotic effects by Limosilactobacillus reuteri
Journal: Microbiome
doi: 10.1186/s40168-021-01128-4
Figure Legend Snippet: Phenotypic and transcriptional distinction between B cell subsets in Peyer’s patches. a Flow cytometry of PPs live CD3 - CD19 + B220 + B lymphocytes (gated as shown in Fig. S1A) and separated into the small and large B cell subsets by forward scatter area and expressed as mean FSC-A (size), and the number of small-B and large-B cells per mg tissue ( n = 6 mice left panel; n = 24 mice right panel). b Imaging flow cytometry of the areas (μm 2 ) of small-B and large-B cell from three independent experiments. c , d Immunohistochemistry of PPs stained with anti-B220 (magenta), anti-CD138 (yellow) and Hoechst (blue) in c or anti-B220 (white), anti-GL7 (green), anti-Ki67 (cyan), anti-IgA (yellow), and anti-IgD (blue) in d . Scale Bars equal 100 μm or 10 μm in closed-up reviews. e Enrichment of gene ontology categories (Biological Process, BP) for genes differentially expressed in large B (upper panel) versus small B cells (lower panel) determined by microarray of lin - CD19 + B220 + cells sorted on FSC-A ( n = 4 samples per group), the number of genes in each functional category is shown. Data were adjusted by false discovery rate control (FDR). f, g Displays of heat maps of genes expression by q-RT-PCR in sorted small and large B cells. The expression was normalized to the mean value of large B and each column represents one sample. h , i Heat maps and histograms depicting expression of surface markers on small and large B cells ( n = 6 mice per group). The frequency of indicated markers (normalized to the mean value of large B cells). i MFI of B cells positive for GL7 and S1PR1, and percentage in each subset. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 using two-tailed Student’s t test
Techniques Used: Flow Cytometry, Imaging, Immunohistochemistry, Staining, Microarray, Functional Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Two Tailed Test
Figure Legend Snippet: L. reuteri expands the GC-like B cell population and reprograms its functional gene signature. a , c Immunohistochemistry of the GL7 + area stained with anti-B220 (magenta), anti-GL7 (green) and anti-Ki67 (cyan). Scale bars equal 100 μm. Representative images of PPs from the control or L. reuteri -treatment are shown in a and c , and the corresponding quantification shown in Fig. S3G, H. b , d Flow cytometry quantification of GL7 + B cells (in CD3 − CD19 + B220 + , n = 5–6 mice per group) and Ki67 + cells ( n = 5–6 mice per group). e In vivo proliferation assay was performed by flow cytometry following i.p . injection of EdU. A histogram depicting EdU expression in GL7 − , GL7 + or EdU + CD19 + B220 + B cells and the number of EdU + GL7 + B cells ( n = 6 mice per group). f Expression of Mki67 , Gsk-3α , and Hif-1α in large GC-like B cells from control or L. reuteri -treated mice (GOI, gene of interest; A.U., arbitrary unit). g q-RT-PCR analysis of gene expression levels ( n = 6 mice per group) and expression of TGFβR1 (microarray analysis). h Flow cytometry analyzing the numbers of TGFβ1 + B cells as well as the TGFβ1 levels (MFI, n = 6–18 mice per group). i The PPs tissue was analyzed for cytokine/chemokine production by Multi-Plex Mesoscale and ELISA, normalized to tissue protein content ( n = 6 mice per group). j Expression of α germline transcripts ( αGT ) in PPs ( n = 6 mice per group). k The numbers of B cells of different subsets in PPs in untreated mice and in response to L. reuteri R2LC and L. reuteri R2LC_ΔADO ( n = 6 mice per group). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01 using Student’s t test or ANOVA with Tukey’s post hoc test
Techniques Used: Functional Assay, Immunohistochemistry, Staining, Flow Cytometry, In Vivo, Proliferation Assay, Injection, Expressing, Reverse Transcription Polymerase Chain Reaction, Microarray, Enzyme-linked Immunosorbent Assay
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