mouse monoclonal antibody against human anln (Danaher Inc)

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Danaher Inc mouse monoclonal antibody against human anln
Mouse Monoclonal Antibody Against Human Anln, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 19347 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue+microarray+based+immunohistochemistry+analysis/mouse+monoclonal+Anti-SOX2+antibody/pmc06686567-100-6-13
Average 99 stars, based on 19347 article reviews

mouse monoclonal antibody against human anln - by Bioz Stars, 2026-10

99/100 stars

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Blocking Assay:

Article Title: Treatment with β-sitosterol ameliorates the effects of cerebral ischemia/reperfusion injury by suppressing cholesterol overload, endoplasmic reticulum stress, and apoptosis
Article Snippet: .. After blocking, the membranes were incubated with rabbit polyclonal anti-GRP78/Bip (1:1000, Abcam, Cat# ab21685, RRID: AB_2119834), rabbit polyclonal anti-Caspase 12 (1:1000, Abcam, Cat# ab62484, RRID: AB_955729), mouse monoclonal anti-Caspase 9 (1:1000, Abcam, Cat# ab115792, RRID: AB_10938323), rabbit polyclonal anti-Caspase 3 (1:1000, Abcam, Cat# ab13847, RRID: AB_443014), mouse polyclonal anti-Bax (1:1000, Abcam, Cat# ab216494), rabbit polyclonal anti-Bcl-2 (1:1000, Abcam, Cat# ab196495, RRID: AB_2924862), goat polyclonal anti-JNK2 (1:1000, Abcam, Cat# ab134567), rabbit polyclonal anti-p-JNK2 (1:1000, Abcam, Cat# ab21685), mouse monoclonal anti-STAT3 (1:1000, Abcam, Cat# ab119352, RRID: AB_10901752), rabbit polyclonal anti-p-STAT3 (1:1000, Abcam, Cat# ab278669, RRID: AB_779085), rabbit monoclonal anti-Niemann-Pick C1 like 1 (NPC1L1) (1:1000, Abcam, Cat# ab124801, RRID: AB_10976493), and rabbit polyclonal anti-β-actin (1:5000, Abcam, Cat# ab8227, RRID: AB_2305186) overnight at 4°C. .. Next, the membranes were washed three times with Tris-buffered saline and Tween 20, then incubated with goat anti-rabbit IgG H&L (HRP) (1:10,000, Abcam, Cat# ab6721, RRID: AB_955447) and goat anti-mouse IgG H&L (HRP) (1:10,000, Abcam, Cat# ab6789, RRID: AB 955439) at room temperature for 1 hour.

Incubation:

Article Title: Cutaneous Toll-like Receptor 9 Pre-Defines Hydroxychloroquine Dosage in Patients with Both Discoid and Subacute Lupus Erythematosus
Article Snippet: .. Antigen retrieval was performed by immersing the slides in EDTA (pH 8.0; 0.01 M) and subjecting them to 97 °C in a water bath for 30 min. Each set of slides was then incubated with specific anti–TLRAbs for 12 h. The first set of slides was incubated with the rabbit monoclonal anti-TLR7 antibody (EPR2088(2); Abcam (Cambridge, UK); dilution of 1:100); the second set of slides was incubated with the mouse monoclonal anti-TLR8 antibody (44C143; Abcam; dilution of 1:100); the third set of slides was incubated with the mouse monoclonal anti-TLR9 antibody (26C593.2; Abcam; dilution of 1:100). .. Polyclonal secondary antibodies conjugated to the horseradish peroxidase (HRP) enzyme (Ultra Vision LP Value Detection System HRP Polymer, Lab Vision, ThermoScientific, USA) were applied to visualize the obtained antigen–antibody complexes, using DAB (3,3′-diaminobenzidine) as the chromogen.

Western Blot:

Article Title: Lysosome damage triggers direct ATG8 conjugation and ATG2 engagement via non-canonical autophagy
Article Snippet: .. Antibodies used were rabbit polyclonal anti-ATG16L1 (Cat#8090, WB 1:1,000; Cell Signalling), rabbit polyclonal anti-LC3A/B (Cat#4108, WB 1:1,000, IF 1:100; Cell Signalling), rabbit polyclonal anti-LC3B (Cat#L7543, RRID:AB_796155, WB 1:1,000; Sigma-Aldrich), rabbit monoclonal anti-ATP6V1A (Cat#ab199326, RRID:AB_2802119, WB 1:2,000; Abcam), mouse monoclonal anti-ATP6V0d1 (Cat#ab56441, WB 1:1,000; Abcam), mouse monoclonal anti-LAMP1 (Cat#555798, IF 1:100; BD Biosciences), mouse monoclonal anti LAMP1 (Cat#611042, WB, 1:500; BD Bioscience), mouse monoclonal anti-GAPDH (Cat#ab8245, RRID:AB_2107448 WB 1:1,000; Abcam), rabbit anti-ATG13 (Cat#6940, WB 1:1,000; Cell Signalling), mouse anti-GFP (Cat#1181446000, WB 1:1,000; Roche), mouse anti-WIPI2 (Cat#MCA5780GA, IF 1:100; BioRad), rabbit anti ATG2B (Cat#25155-1-AP, WB 1:1,000; ProteinTech), rabbit anti-ATG2A (Cat#23226-1-AP, WB 1:1,000; ProteinTech), mouse anti-Galectin 3 (Cat#32790, IF 1:100; Scbt), rabbit anti-b tubulin (Cat#2128, WB 1:1,000; Cell Signalling), rabbit monoclonal anti-Rab10 (Cat#8127, WB 1:1,000; Cell Signalling), rabbit monoclonal anti-pT73 Rab10 (Cat#ab230261, RRID:AB_2811274, WB 1:1,000; Abcam), Alexa Fluor 488 polyclonal goat anti-rabbit IgG (Cat#A-11034, IF 1:500; Thermo Fisher Scientific), Alexa Fluor 568 polyclonal goat anti-mouse IgG (Cat#A-11004, IF 1:500; Thermo Fisher Scientific), Alexa Fluor 568 polyclonal goat anti-rabbit IgG (Cat#A-11011, RRID:AB_143157, IF 1:500; Thermo Fisher Scientific), HRP-conjugated anti-rabbit IgG (Cat#7074, WB 1:2,000; Cell Signalling), and HRP-conjugated anti-mouse IgG (Cat#7076, RRID:AB_330924, WB 1:2,000; Cell Signaling Technology). .. Reagents and chemicals used were LLOMe (L7393; Sigma-Aldrich), GPN (SC-252858; Scbt), BafA1 (1334; Tocris), PP242 (4257; Tocris), Monensin (M5273; Sigma-Aldrich), human serum (H2918; Sigma-Aldrich), Zymosan (Z4250; Sigma-Aldrich), murine IFNγ (315-05; Peprotech), DAPI (D9542; Sigma-Aldrich), IN-1 (17392; Caymen Chemical), MLi2 (5756; Tocris), and saliphenylhalamide (SaliP), and TRPML1 agonist C8 and ATG7 inhibitor ATG7-IN-3 were kindly provided by Casma Therapeutics.

Marker:

Article Title: Functional brain region-specific neural spheroids for modeling neurological diseases and therapeutics screening
Article Snippet: For (2) neuronal cell-type markers, polyclonal chicken anti-Tyrosine Hydroxylase (Abcam, cat# ab76442, 1:500) was used to stain dopaminergic neurons, monoclonal mouse anti-vGluT1 (Abcam, cat# ab242204, 1:250) was used to stain glutamatergic neurons, and polyclonal rabbit anti-parvalbumin (Abcam, cat# ab11427, 1:500) was used to stain GABAergic neurons. .. For (3), mouse monoclonal anti-bassoon antibody (Abcam, cat# ab82958, 1:100) was used as a presynaptic marker while rabbit polyclonal anti-homer1 antibody (Abcam, cat# ab97593, 1:100) was used as a postsynaptic marker. .. Secondary antibodies used in this study include goat anti-mouse Alexa Fluor 488, goat anti-rabbit Alexa Fluor 488, goat anti-chicken Alexa Fluor 568, goat anti-rabbit Alexa Fluor 568, goat anti-mouse, Alexa Fluor 568, goat anti-rabbit Alexa Fluor 647, goat anti-chicken Alexa Fluor 647, and goat anti-mouse Alexa Fluor 647 (Invitrogen, cat# A28175, A11008, A11041, A11011, A11004, A32733, A32933, A32728, respectively).

Staining:

Article Title: Progesterone receptor-Grb2 interaction is associated with better outcomes in breast cancer.
Article Snippet: In addition to mediating nuclear transcription, PR mediates extranuclear functions mainly through the PR polyproline domain (PPD) interaction with the SH3 domain of cytoplasmic signaling molecules.. PR-PPD-SH3 interaction inhibits EGF-mediated signaling and decreases lung cancer cell proliferation.. Grb2 is an essential adaptor molecule with an SH2 domain flanked by two SH3 domains.

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