recombinant human tgfβ1 (R&D Systems)
R&D Systems is a verified supplier 
R&D Systems manufactures this product 
97
Structured Review
R&D Systems
recombinant human tgfβ1
Recombinant Human Tgfβ1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1997 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue+microarray+based+immunohistochemistry+analysis/Recombinant+Human+TGF-beta+1+Protein/pmc04309391-45-23-42
Average 97 stars, based on 1997 article reviews
Recombinant Human Tgfβ1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1997 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue+microarray+based+immunohistochemistry+analysis/Recombinant+Human+TGF-beta+1+Protein/pmc04309391-45-23-42
Average 97 stars, based on 1997 article reviews
recombinant human tgfβ1 - by Bioz Stars,
2026-11
97/100 stars
Images
1) Product Images from "Activin A balance regulates epithelial invasiveness and tumorigenesis"
Article Title: Activin A balance regulates epithelial invasiveness and tumorigenesis
Journal: Laboratory investigation; a journal of technical methods and pathology
doi: 10.1038/labinvest.2014.97
Figure Legend Snippet: (a) Immunohistochemistry staining with antibody against phosphorylated Smad2 (pSmad2) and TGFβ receptor II (TβRII) showed increased nuclear signal for pSmad2 in the invasive ECdnT organotypic cultures. Scale bar 50 micron. (b) Analysis of immunohistochemistry staining for TβRII and pSmad2 in 83 ESCC cases in a tissue microarray shows no significant correlation. Fisher’s exact test, two tailed p= 0.3182. (c) Five paired normal adjacent and ESCC tissues (GSE17531) were analyzed for INHBA mRNA expression, which identified upregulation of INHBA in four ESCC samples. (d) Waterfall plot of a publically available dataset (GSE23400) represented upregulation of INHBA in the ESCC (grey bars) samples vs. normal (black bars).
Techniques Used: Immunohistochemistry, Staining, Microarray, Two Tailed Test, Expressing
Figure Legend Snippet: (a) Esophageal epithelial cells expressing wild-type full-length E-cadherin (E), dominant-negative mutant E-cadherin (EC) or dominant-negative mutant E-cadherin and TGFβ receptor II (ECdnT) were grown in organotypic cultures with either fetal esophageal fibroblasts (FEF) or cancer-associated fibroblasts (CAF) embedded in the underlying matrix. Immunofluorescence staining with antibody against αSMA (green) and podoplanin (red) showed similar expression pattern in the cultures. Scale bar is 50 micron. (b) Activin A concentration in conditioned media from organotypic cultures is higher in invasive cultures as measured using indirect ELISA. * p=0.003, ** p= 0.005, *** p=0.03 (c) Stimulation of epithelial cells with Act A in monolayer plastic culture demonstrated phosphorylation of Smad. Neutralizing antibody against Activin (nAb) prevented the induction of pSmad2 by Act A. Following stimulation with Act A or with conditioned media from organotypic culture increased expression of vimentin was detected after 48 hours by Western Blot. The increase was reversed in the presence of neutralizing antibody (nAb). (d) Inhibition with the Act A antagonist, Follistatin, or a pan-TGFβ inhibitor A83-01 suppressed MMP-9 secretion in E, EC and ECdnT cells as measured by gelatin zymography. Upper bands reflect pro-MMP, lower bands activated, cleaved MMP (arrow).
Techniques Used: Expressing, Dominant Negative Mutation, Immunofluorescence, Staining, Concentration Assay, Indirect ELISA, Phospho-proteomics, Western Blot, Inhibition, Zymography
Figure Legend Snippet: (a) Esophageal epithelial cells expressing wild-type full-length E-cadherin (E), dominant-negative mutant E-cadherin (EC) or dominant-negative mutant E-cadherin and TGFβ receptor II (ECdnT) were grown in organotypic cultures in the presence of recombinant Activin A (Act A), its antagonist Follistatin or a neutralizing antibody against Activin A (nAb); H&E staining. Stimulation with Act A inhibited invasion of E and EC cells, but failed to suppress ECdnT cell invasion. Follistatin increased cell invasion in all cell types, while the neutralizing antibody prevented invasion of E and EC cells, without an effect on ECdnT cells. (b) Immunohistochemistry staining with ki67-antibody showed no differences in cell proliferation. Scale bars are 50 micron. (c) Indirect ELISA with antibody against Act A measured increased levels after addition of recombinant Act A in fibroblasts (FEF) and ECdnT. Untreated ECdnT cells (Control) secreted higher baseline levels of Act A than FEF, which were reduced by Follistatin. (d) TGFβ1 concentration was increased in response to stimulation with Act A and overall baseline secretion was higher in control ECdnT cells than fibroblasts as measured by indirect ELISA. Follistatin inhibited TGFβ1 secretion.
Techniques Used: Expressing, Dominant Negative Mutation, Recombinant, Staining, Immunohistochemistry, Indirect ELISA, Control, Concentration Assay
Figure Legend Snippet: (a) Separating the cellular matrix and epithelium of the organotypic cultures growing ECdnT cells through a collagen I layer, dashed lines, prevented cell invasion in the absence (control) and presence of Act A (+Act A). When the cellular matrix of the organotypic culture was treated with puromycin to kill the embedded fibroblasts before the ECdnT cells were seeded, epithelial formation occurred but invasion was inhibited with and without Act A stimulation. (b) Treatment of ECdnT organotypic cultures with a pan-MMP inhibitor, GM6001, suppressed cell invasion, which was not restored in the presence of Act A. Untreated (no tx) control ECdnT cells in organotypic culture invaded into the underlying matrix. TGFβ1 treatment inhibited epithelial cell invasion. Scale bars are 50 micron. (c) Immunohistochemistry showed nuclear localization of phosphorylated Smad (pSmad2, red) in control and Act A stimulated conditions. Collagen IV, red, was disrupted in invasive cultures after Act A treatment. Loss of the fibroblasts (FEF), labeled green with antibody against vimentin (no staining in the lower panels), had no effect on the nuclear localization of pSmad2. The collagen IV layer was not disrupted in non-invasive cultures in the absence of FEFs.
Techniques Used: Control, Immunohistochemistry, Labeling, Staining
Related Articles
Cell Culture:Article Title: Aberrant laminin signaling drives melanocyte dedifferentiation and unveils a tractable therapeutic target in vitiligo. Article Snippet: .. For mesodermal differentiation, cells were cultured in a 1:1 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM; #D5921, SigmaAldrich) and F-12 (#N4888, Sigma-Aldrich), supplemented with 1× nonessential amino acid solution (#139-15651, FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 60 pM AR TI CL E IN Modification:Article Title: Aberrant laminin signaling drives melanocyte dedifferentiation and unveils a tractable therapeutic target in vitiligo. Article Snippet: .. For mesodermal differentiation, cells were cultured in a 1:1 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM; #D5921, SigmaAldrich) and F-12 (#N4888, Sigma-Aldrich), supplemented with 1× nonessential amino acid solution (#139-15651, FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 60 pM AR TI CL E IN Recombinant:Article Title: Aberrant laminin signaling drives melanocyte dedifferentiation and unveils a tractable therapeutic target in vitiligo. Article Snippet: .. For mesodermal differentiation, cells were cultured in a 1:1 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM; #D5921, SigmaAldrich) and F-12 (#N4888, Sigma-Aldrich), supplemented with 1× nonessential amino acid solution (#139-15651, FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 60 pM AR TI CL E IN Article Title: TGF-β1 modulates PFC glial cell activation to facilitate exercise-induced analgesia in mice with spared nerve injury Article Snippet: .. To validate the specificity of the TGF-β1 antibody, Article Title: Induction of fibrosis in human kidney organoids delineates mechanisms and therapeutic targets of fibrotic kidney disease Article Snippet: .. To induce fibrosis in kidney organoids, the organoids were treated with 10 ng/ml Article Title: N-glycosylation is essential for aberrant Tfh cells differentiation in systemic lupus erythematosus. Article Snippet: Aberrant differentiation of T follicular helper (Tfh) cells contributes to the pathogenesis of autoimmune diseases such as systemic lupus erythematosus (SLE).. However, whether Tfh cell differentiation is regulated by glycosylation remains unclear.. In this study, we found that inhibition of N-glycosylation completely blocked Tfh cell differentiation. Article Title: Single-cell morphodynamical trajectories enable prediction of gene expression accompanying cell state change. Article Snippet: .. Cells were maintained in DMEM/ F12 supplemented with insulin, sodium selenite, hydrocortisone, β-estrogen, and transferrin, and maintained in 5% C02 at 37◦C.111 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Recombinant Human EGF Protein, CF R&D Systems Cat#236-EG Recombinant Human Oncostatin M (HEK-293-expressed) Protein R&D Systems Positive Control:Article Title: TGF-β1 modulates PFC glial cell activation to facilitate exercise-induced analgesia in mice with spared nerve injury Article Snippet: .. To validate the specificity of the TGF-β1 antibody, Sequencing:Article Title: Single-cell morphodynamical trajectories enable prediction of gene expression accompanying cell state change. Article Snippet: .. Cells were maintained in DMEM/ F12 supplemented with insulin, sodium selenite, hydrocortisone, β-estrogen, and transferrin, and maintained in 5% C02 at 37◦C.111 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Recombinant Human EGF Protein, CF R&D Systems Cat#236-EG Recombinant Human Oncostatin M (HEK-293-expressed) Protein R&D Systems RNA sequencing:Article Title: Single-cell morphodynamical trajectories enable prediction of gene expression accompanying cell state change. Article Snippet: .. Cells were maintained in DMEM/ F12 supplemented with insulin, sodium selenite, hydrocortisone, β-estrogen, and transferrin, and maintained in 5% C02 at 37◦C.111 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Recombinant Human EGF Protein, CF R&D Systems Cat#236-EG Recombinant Human Oncostatin M (HEK-293-expressed) Protein R&D Systems Expressing:Article Title: Single-cell morphodynamical trajectories enable prediction of gene expression accompanying cell state change. Article Snippet: .. Cells were maintained in DMEM/ F12 supplemented with insulin, sodium selenite, hydrocortisone, β-estrogen, and transferrin, and maintained in 5% C02 at 37◦C.111 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Recombinant Human EGF Protein, CF R&D Systems Cat#236-EG Recombinant Human Oncostatin M (HEK-293-expressed) Protein R&D Systems Live Cell Imaging:Article Title: Single-cell morphodynamical trajectories enable prediction of gene expression accompanying cell state change. Article Snippet: .. Cells were maintained in DMEM/ F12 supplemented with insulin, sodium selenite, hydrocortisone, β-estrogen, and transferrin, and maintained in 5% C02 at 37◦C.111 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Recombinant Human EGF Protein, CF R&D Systems Cat#236-EG Recombinant Human Oncostatin M (HEK-293-expressed) Protein R&D Systems HMT Assay:Article Title: Single-cell morphodynamical trajectories enable prediction of gene expression accompanying cell state change. Article Snippet: .. Cells were maintained in DMEM/ F12 supplemented with insulin, sodium selenite, hydrocortisone, β-estrogen, and transferrin, and maintained in 5% C02 at 37◦C.111 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Recombinant Human EGF Protein, CF R&D Systems Cat#236-EG Recombinant Human Oncostatin M (HEK-293-expressed) Protein R&D Systems Plasmid Preparation:Article Title: Single-cell morphodynamical trajectories enable prediction of gene expression accompanying cell state change. Article Snippet: .. Cells were maintained in DMEM/ F12 supplemented with insulin, sodium selenite, hydrocortisone, β-estrogen, and transferrin, and maintained in 5% C02 at 37◦C.111 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Recombinant Human EGF Protein, CF R&D Systems Cat#236-EG Recombinant Human Oncostatin M (HEK-293-expressed) Protein R&D Systems Software:Article Title: Single-cell morphodynamical trajectories enable prediction of gene expression accompanying cell state change. Article Snippet: .. Cells were maintained in DMEM/ F12 supplemented with insulin, sodium selenite, hydrocortisone, β-estrogen, and transferrin, and maintained in 5% C02 at 37◦C.111 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Recombinant Human EGF Protein, CF R&D Systems Cat#236-EG Recombinant Human Oncostatin M (HEK-293-expressed) Protein R&D Systems Fluorescence:Article Title: Single-cell morphodynamical trajectories enable prediction of gene expression accompanying cell state change. Article Snippet: .. Cells were maintained in DMEM/ F12 supplemented with insulin, sodium selenite, hydrocortisone, β-estrogen, and transferrin, and maintained in 5% C02 at 37◦C.111 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Recombinant Human EGF Protein, CF R&D Systems Cat#236-EG Recombinant Human Oncostatin M (HEK-293-expressed) Protein R&D Systems |
