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e coli atcc 25922  (ATCC)


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    Structured Review

    ATCC e coli atcc 25922
    MIC values of SET-M33D peptide on selected strains of major pathogen species.
    E Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5672 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tissue+expression+of+vim/Escherichia+coli/pmc07760307-41-0-2
    Average 99 stars, based on 5672 article reviews
    e coli atcc 25922 - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Antibacterial and Anti-Inflammatory Activity of an Antimicrobial Peptide Synthesized with D Amino Acids"

    Article Title: Antibacterial and Anti-Inflammatory Activity of an Antimicrobial Peptide Synthesized with D Amino Acids

    Journal: Antibiotics

    doi: 10.3390/antibiotics9120840

    MIC values of SET-M33D peptide on selected strains of major pathogen species.
    Figure Legend Snippet: MIC values of SET-M33D peptide on selected strains of major pathogen species.

    Techniques Used: Bla VIM Assay

    Frequency of selection (mean ± SD) of resistant mutants on three reference strains after exposure to SET-M33D and to colistin.
    Figure Legend Snippet: Frequency of selection (mean ± SD) of resistant mutants on three reference strains after exposure to SET-M33D and to colistin.

    Techniques Used: Selection, Mutagenesis

    Effect of SET-M33D on protein release or gene expression of proinflammatory cytokines. ( A , B ) ELISA measurement of TNF-α and IL-6 produced by RAW264.7 cells after stimulation with LPS from P. aeruginosa (20 ng/mL) or LTA from S. aureus (2 µg/mL) in the presence of different concentration of SET-M33D. Data is expressed as percentage inhibition of cytokines with respect to LPS or LTA values (100%). Values are the mean ± SD of five independent experiments (n = 5). IC50s are reported in the text. ( C – L ) Gene expression of proinflammatory cytokines MIP1, KC, IP10, TNF-α, and IL-6 was analyzed by RT-PCR. RAW264.7 cells were stimulated with LPS from E. coli ( C – G ) or with LTA from S. aureus ( H – L ) in presence of SET-M33D at 10 or 1 µM. Densitometric analysis of cDNA bands (pictures under the columns) was carried out using ImageJ software. The reduction of cDNA is indicated as fold change with respect to control ± SD of two independent experiments (n = 2). * p < 0.05, ** p < 0.01 calculated using Student’s t -test with GraphPad Prism.
    Figure Legend Snippet: Effect of SET-M33D on protein release or gene expression of proinflammatory cytokines. ( A , B ) ELISA measurement of TNF-α and IL-6 produced by RAW264.7 cells after stimulation with LPS from P. aeruginosa (20 ng/mL) or LTA from S. aureus (2 µg/mL) in the presence of different concentration of SET-M33D. Data is expressed as percentage inhibition of cytokines with respect to LPS or LTA values (100%). Values are the mean ± SD of five independent experiments (n = 5). IC50s are reported in the text. ( C – L ) Gene expression of proinflammatory cytokines MIP1, KC, IP10, TNF-α, and IL-6 was analyzed by RT-PCR. RAW264.7 cells were stimulated with LPS from E. coli ( C – G ) or with LTA from S. aureus ( H – L ) in presence of SET-M33D at 10 or 1 µM. Densitometric analysis of cDNA bands (pictures under the columns) was carried out using ImageJ software. The reduction of cDNA is indicated as fold change with respect to control ± SD of two independent experiments (n = 2). * p < 0.05, ** p < 0.01 calculated using Student’s t -test with GraphPad Prism.

    Techniques Used: Gene Expression, Enzyme-linked Immunosorbent Assay, Produced, Concentration Assay, Inhibition, Reverse Transcription Polymerase Chain Reaction, Software, Control

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    Activity Assay:

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    Article Snippet: .. Antibacterial activity of CSGHs was evaluated against a panel of ten bacterial strains, including five Gram-positive species: Bacillus cereus (ATCC 11778), Listeria monocytogenes (ATCC 19117), Micrococcus luteus (ATCC 4698), Staphylococcus aureus (ATCC 25923), and Enterococcus faecium (ATCC 29212), and five Gram-negative species: Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 13883), Pseudomonas fluorescens (ATCC 13525), Salmonella enterica (ATCC 10708), and Salmonella typhi (ATCC 19430). ..

    Control:

    Article Title: Antimicrobial resistance and genomic characteristics of avian Pasteurella multocida
    Article Snippet: .. Escherichia coli ATCC 25922 was used as the quality control strain, and results were interpreted according to CLSI standards (CLSI M100-ED32:2022). ..

    Article Title: Fifty years of chicken-source Salmonella in China: Evolutionary shifts in epidemiology, antimicrobial resistance, and genomic lineages
    Article Snippet: .. Escherichia coli ATCC 25922 and ATCC 35218 were used as quality control strains. ..

    Article Title: The synthetic peptide GATR-3 shows significant antibacterial and biofilm-inhibition activity against shellfish- and oyster-associated bacteria Vibrio vulnificus and Vibrio parahaemolyticus
    Article Snippet: .. Against the control strain E. coli ATCC 25922, GATR-3 was extremely potent, with an EC50 of 4.44 × 10−5 μM (1.27 × 10−4 μg/mL), confirming its broad-spectrum and potent efficacy. ..

    Article Title: Effective treatment of multidrug-resistant Riemerella anatipestifer infection in ducks using a compound Chinese herbal medicine in drinking water
    Article Snippet: Antibiotic discs (Hangzhou Microbial Reagent, China) were placed on the agar surface, and the plates were incubated at 37°C for 24 h. Inhibition zone diameters were measured and interpreted in accordance with clinical breakpoints. .. Escherichia coli ATCC 25922 was used as the quality control strain. ..

    Article Title: Genetic diversity, serotype, and antimicrobial profiles of Riemerella anatipestifer isolated from ducks and chickens in Thailand
    Article Snippet: The antimicrobial susceptibility of R. anatipestifer isolates was determined using the agar dilution method, in accordance with the Clinical and Laboratory Standards Institute guidelines ( ). .. Escherichia coli ATCC® 25922 and Pseudomonas aeruginosa ATCC® 27853 were used as quality control reference strains. .. The 11 antibiotics tested in this study were amoxicillin, ampicillin, ceftriaxone, gentamicin, streptomycin, doxycycline, tetracycline, chloramphenicol, colistin, sulfamethoxazole, and trimethoprim (Sigma-Aldrich®, USA).



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