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elisa kit  (R&D Systems)


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    Structured Review

    R&D Systems elisa kit
    Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tim3/Human+TIM-3+Quantikine+ELISA+Kit/pm36591283-74-7-9
    Average 93 stars, based on 16 article reviews
    elisa kit - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Soluble Tim-3 serves as a tumor prognostic marker and therapeutic target for CD8 + T cell exhaustion and anti-PD-1 resistance.
    Article Snippet: .. Serum sTim-3 concentrations in patients and tumor-bearingmice weremeasured using the human enzyme-linked immunosorbent assay (ELISA) kit (cat#DTIM30, R&D Systems, Inc., Minneapolis, MN, USA). .. d The RNA-seq data generated in this study have been deposited in the National Center for Biotechnology Information (NCBI) database under BioProject accession number SRA: PRJNA1135313.

    Article Title: Soluble TIM-3 and galectin-9 predict survival in gastric and gastroesophageal junction cancer
    Article Snippet: Seventeen different soluble immune checkpoint proteins were measured using Luminex xMAP technology (RRID: SCR_018025 ) as described before (data for sTIM-3 shown in A and S3B). .. Enzyme-linked immunosorbent assay (ELISA) was used for validation according to the manufacturer’s protocol for quantification of sTIM-3 and Gal-9 (Human TIM-3 ELISA Kit – Quantikine, Catalog #: DTIM30, Human Gal-9 ELISA Kit – Quantikine Catalog #: DGAL90, R&D Systems, Minneapolis, USA). ..

    Article Title: Mesothelin methylation, soluble mesothelin related protein levels and inflammation profiling in workers chronically exposed to naturally occurring asbestos fibers.
    Article Snippet: .. Levels of serum interleukin-1β (IL-1β), IL-6, IL-8, and TNF-α were determined utilizing sensitive quantitative sandwich enzyme-linked immunosorbent assays (Quantikine ELISA Kit, R&D systems, USA) [22,33]. .. Soluble mesothelin related protein (SMRP) levels were quantified utilizing the MESOMARKTM ELISA kit (Fujirebio Diagnostics, Inc., Malvern, PA, USA) following the manufacturer’s guidelines.

    Article Title: Soluble TIM-3 and galectin-9 predict survival in gastric and gastroesophageal junction cancer
    Article Snippet: Seventeen different soluble immune checkpoint proteins were measured using 452 Luminex xMAP technology (RRID:SCR_018025) as described before (data for sTIM-3 shown in 453 Supplementary Fig. S3A, B)53. .. Enzyme-linked immunosorbent assay (ELISA) was used for 454 validation according to the manufacturer’s protocol for quantification of sTIM-3 and Gal-9 (Human 455 TIM-3 ELISA Kit – Quantikine, Catalog #: DTIM30, Human Gal-9 ELISA Kit – Quantikine Catalog 456 #: DGAL90, R&D Systems, Minneapolis, USA). ..

    Article Title: Serum soluble Tim‑3 is elevated in patients with cervical cancer and is higher in advanced clinical stages
    Article Snippet: .. The serum concentration of sTim-3 was determined using a Quantikine Human Tim-3 enzyme-linked immunosorbent assay (ELISA) kit (cat. no. DTIM30; R&D Systems Inc.), according to the manufacturer's instructions. ..

    Article Title: TIM-3 antagonists for the treatment and diagnosis of cancers
    Article Snippet: .. To compare soluble TIM3 expression in the serum of healthy subjects and cancer patients, frozen serum samples from 20 normal healthy volunteers, 20 colon, 20 kidney and 20 lung cancer patients were thawed on ice and tested at 1:4 dilution for soluble Tim-3 with a commercially available ELISA kit (Quantikine ELISA, cat #DTIM30, R&D Systems). ..

    Article Title: Serum soluble Tim‑3 is elevated in patients with cervical cancer and is higher in advanced clinical stages.
    Article Snippet: .. The serum concentration of sTim‐3 was determined using a Quantikine Human Tim‐3 enzyme‐linked immunosorbent assay (ELISA) kit (cat. no. DTIM30; R&D Systems Inc.), according to the manufacturer's instructions. ..

    Article Title: Soluble Tim-3 serves as a tumor prognostic marker and therapeutic target for CD8 T cell exhaustion and anti-PD-1 resistance
    Article Snippet: .. Serum sTim-3 concentrations in patients and tumor-bearingmice weremeasured using the human enzyme-linked immunosorbent assay (ELISA) kit (cat#DTIM30, R&D Systems, Inc., Minneapolis, MN, USA). .. The spontaneous orthotopic HCC mouse model The SB100 transposase-expressing vector pCMV(CAT)T7-SB100 was a gift from Zsuzsanna Izsvak (Addgene plasmid # 34879).

    Biomarker Discovery:

    Article Title: Soluble TIM-3 and galectin-9 predict survival in gastric and gastroesophageal junction cancer
    Article Snippet: Seventeen different soluble immune checkpoint proteins were measured using Luminex xMAP technology (RRID: SCR_018025 ) as described before (data for sTIM-3 shown in A and S3B). .. Enzyme-linked immunosorbent assay (ELISA) was used for validation according to the manufacturer’s protocol for quantification of sTIM-3 and Gal-9 (Human TIM-3 ELISA Kit – Quantikine, Catalog #: DTIM30, Human Gal-9 ELISA Kit – Quantikine Catalog #: DGAL90, R&D Systems, Minneapolis, USA). ..

    Article Title: Soluble TIM-3 and galectin-9 predict survival in gastric and gastroesophageal junction cancer
    Article Snippet: Seventeen different soluble immune checkpoint proteins were measured using 452 Luminex xMAP technology (RRID:SCR_018025) as described before (data for sTIM-3 shown in 453 Supplementary Fig. S3A, B)53. .. Enzyme-linked immunosorbent assay (ELISA) was used for 454 validation according to the manufacturer’s protocol for quantification of sTIM-3 and Gal-9 (Human 455 TIM-3 ELISA Kit – Quantikine, Catalog #: DTIM30, Human Gal-9 ELISA Kit – Quantikine Catalog 456 #: DGAL90, R&D Systems, Minneapolis, USA). ..

    Concentration Assay:

    Article Title: Serum soluble Tim‑3 is elevated in patients with cervical cancer and is higher in advanced clinical stages
    Article Snippet: .. The serum concentration of sTim-3 was determined using a Quantikine Human Tim-3 enzyme-linked immunosorbent assay (ELISA) kit (cat. no. DTIM30; R&D Systems Inc.), according to the manufacturer's instructions. ..

    Article Title: Serum soluble Tim‑3 is elevated in patients with cervical cancer and is higher in advanced clinical stages.
    Article Snippet: .. The serum concentration of sTim‐3 was determined using a Quantikine Human Tim‐3 enzyme‐linked immunosorbent assay (ELISA) kit (cat. no. DTIM30; R&D Systems Inc.), according to the manufacturer's instructions. ..

    Expressing:

    Article Title: TIM-3 antagonists for the treatment and diagnosis of cancers
    Article Snippet: .. To compare soluble TIM3 expression in the serum of healthy subjects and cancer patients, frozen serum samples from 20 normal healthy volunteers, 20 colon, 20 kidney and 20 lung cancer patients were thawed on ice and tested at 1:4 dilution for soluble Tim-3 with a commercially available ELISA kit (Quantikine ELISA, cat #DTIM30, R&D Systems). ..



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    tim3  (R&D Systems)
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    R&D Systems tim3
    (A) A UMAP showing the integration of tumour and benign cells of the TME, and healthy lymph node data from two public studies (PMID35027729, PMID39566559). Cells are coloured according to the data source, and scANVI-predicted cell types are shown in (B), (C) and (D). Images of an ALK+ ALCL case colored by Cell2location-predicted cell type abundances in each Visium spot. (C) Predictions for the locations of malignant cells, <t>TIM3+</t> T cells (T_TIM3+) and cytotoxic T cells (T_CD8+_cytotoxic), (D) Predictions for the locations of dark zone B cells (B_GC_DZ), light zone B cells (B_GC_LZ) and follicular dendritic cells (FDC). (E) A dotplot showing non-negative matrix factorisation (NMF) factors, where each factor is a group of co-localized cell types. In the dotplot, the size and color density represent the loading of each cell type in each factor. (F) Staining of TIM3 and CD30 expression on two representative ALK+ ALCL cases (G) Summary of the % TIM3+ infiltrating T lymphocytes in each of the 9 ALK+ ALCL cases analysed.
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    Image Search Results


    (A) A UMAP showing the integration of tumour and benign cells of the TME, and healthy lymph node data from two public studies (PMID35027729, PMID39566559). Cells are coloured according to the data source, and scANVI-predicted cell types are shown in (B), (C) and (D). Images of an ALK+ ALCL case colored by Cell2location-predicted cell type abundances in each Visium spot. (C) Predictions for the locations of malignant cells, TIM3+ T cells (T_TIM3+) and cytotoxic T cells (T_CD8+_cytotoxic), (D) Predictions for the locations of dark zone B cells (B_GC_DZ), light zone B cells (B_GC_LZ) and follicular dendritic cells (FDC). (E) A dotplot showing non-negative matrix factorisation (NMF) factors, where each factor is a group of co-localized cell types. In the dotplot, the size and color density represent the loading of each cell type in each factor. (F) Staining of TIM3 and CD30 expression on two representative ALK+ ALCL cases (G) Summary of the % TIM3+ infiltrating T lymphocytes in each of the 9 ALK+ ALCL cases analysed.

    Journal: bioRxiv

    Article Title: Single-Cell Profiling Reveals Developmental Trajectories and identifies SYK and TIM3 as Targets in some T Cell Lymphomas

    doi: 10.64898/2026.03.27.714741

    Figure Lengend Snippet: (A) A UMAP showing the integration of tumour and benign cells of the TME, and healthy lymph node data from two public studies (PMID35027729, PMID39566559). Cells are coloured according to the data source, and scANVI-predicted cell types are shown in (B), (C) and (D). Images of an ALK+ ALCL case colored by Cell2location-predicted cell type abundances in each Visium spot. (C) Predictions for the locations of malignant cells, TIM3+ T cells (T_TIM3+) and cytotoxic T cells (T_CD8+_cytotoxic), (D) Predictions for the locations of dark zone B cells (B_GC_DZ), light zone B cells (B_GC_LZ) and follicular dendritic cells (FDC). (E) A dotplot showing non-negative matrix factorisation (NMF) factors, where each factor is a group of co-localized cell types. In the dotplot, the size and color density represent the loading of each cell type in each factor. (F) Staining of TIM3 and CD30 expression on two representative ALK+ ALCL cases (G) Summary of the % TIM3+ infiltrating T lymphocytes in each of the 9 ALK+ ALCL cases analysed.

    Article Snippet: Following heat-mediated antigen retrieval, consecutive sections were stained for TIM3 (MAB23652, R&D Systems) and CD30 (M0751, Agilent Dako) both at a 1:50 dilution.

    Techniques: Staining, Expressing