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DIAGENODE DIAGNOSTICS bioruptor
Bioruptor, supplied by DIAGENODE DIAGNOSTICS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thruplex+dna-seq+12s+kit/bioruptor/pm35411071-273-6-7
Average 90 stars, based on 1 article reviews
bioruptor - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Lysis:

Article Title: Chromatin interaction-based annotation of regulatory elements reveals dynamic promoter-enhancer interactions in lymphocyte development
Article Snippet: Nuclei were isolated by incubation in Nuclei Isolation buffer (50 mM Tris, pH 8, 60 mM KCl, and 0.5% NP40) + protease inhibitor mixture (PIC) (Roche Diagnostics) for 10 min on ice. .. Pelleted nuclei were resuspended in Lysis buffer (0.5% SDS, 10 mM EDTA, 0.5 mM EGTA, and 50 mM Tris–HCl (pH 8))) + PIC and sonicated on a Bioruptor (Diagenode), followed by pelleting of debris. .. The supernatant was diluted 5× in HBSS (Lonza) + PIC and 2× radioimmunoprecipitation assay buffer (20 mM Tris–HCl (pH 7.5), 2 mM EDTA, 2% Triton X-100, 0.1% SDS, 0.2% sodium deoxycholate, and 200 mM NaCl) + PIC.

Sonication:

Article Title: Chromatin interaction-based annotation of regulatory elements reveals dynamic promoter-enhancer interactions in lymphocyte development
Article Snippet: Nuclei were isolated by incubation in Nuclei Isolation buffer (50 mM Tris, pH 8, 60 mM KCl, and 0.5% NP40) + protease inhibitor mixture (PIC) (Roche Diagnostics) for 10 min on ice. .. Pelleted nuclei were resuspended in Lysis buffer (0.5% SDS, 10 mM EDTA, 0.5 mM EGTA, and 50 mM Tris–HCl (pH 8))) + PIC and sonicated on a Bioruptor (Diagenode), followed by pelleting of debris. .. The supernatant was diluted 5× in HBSS (Lonza) + PIC and 2× radioimmunoprecipitation assay buffer (20 mM Tris–HCl (pH 7.5), 2 mM EDTA, 2% Triton X-100, 0.1% SDS, 0.2% sodium deoxycholate, and 200 mM NaCl) + PIC.

Article Title: Induction of host genes by nested genes during C. elegans development
Article Snippet: The embryo pellet was resuspended in cold 4°C lysis buffer (0.5% Nonidet P40 substitute, 50 mM Tris/HCl pH7.4, 100 mM KCl, 1 mM MgCl2, 1 mM EGTA, 10% Glycerol, 1 mM DTT, cOmplete Mini Protease Inhibitor Cocktail (Roche #11836153001)), and stored at -80°C. .. To extract proteins, the sample was thawed on ice and sonicated with a Diagenode Bioruptor (an average of 15 cycles, 30 sec ON - 30 sec OFF, amplitude HIGH, 4°C). ..

Article Title: Enhancer profiling uncovers Jmjd1c as an essential suppressor in neuropathic pain by targeting Socs3
Article Snippet: .. Briefly, 1 × 10 7 nerve cells were cross-linked with formaldehyde at a final concentration of 1 %, and then the cells were subjected to sonication using a Diagenode Bioruptor to generate DNA fragments in the 200–500 bp size range, followed by immunoprecipitation using antibodies specific to KLF15 (Abcam, ab2647) and a control normal immunoglobulin G (IgG) (CST, 2729S). .. Briefly, 1 × 10 7 nerve cells were cross-linked with formaldehyde at a final concentration of 1 %, and then the cells were subjected to sonication using a Diagenode Bioruptor to generate DNA fragments in the 200–500 bp size range, followed by immunoprecipitation using antibodies specific to KLF15 (Abcam, ab2647) and a control normal immunoglobulin G (IgG) (CST, 2729S).

other:

Article Title: The potassium channel K 2P 2.1 shapes the morphology and function of brain endothelial cells via actin network remodeling
Article Snippet: Staining of Cfl1 and pCfl1, rabbit anti-Cofilin 1 (Abcam # ab42824, 1:400) and rabbit anti-p (S3) -Cofilin 1 (Abcam # ab12866, 1:400) were used with AF488 goat anti-rabbit secondary antibody (Invitrogen # A11034, 1:500).

Article Title: TIGAR DEFICIENCY ENHANCES CARDIAC RESILIENCE THROUGH EPIGENETIC PROGRAMMING OF PARKIN EXPRESSION
Article Snippet: The minced tissue was cross-linked with 1% formaldehyde (ThermoFisher Scientific, #28908) and processed for chromatin isolation using the ChromaFlash Chromatin Extraction Kit (EpigenTek, P-2001).

Sampling:

Article Title: Mitogenome data of Hylobates abbotti Kloss, 1929 (Abbott’s Gray Gibbon)
Article Snippet: .. Data collection , Buccal swab DNA sampling DNA extraction: Genomic DNA extracted using QIAamp Blood & Tissue Kit (Qiagen, Germany) Library Preparation and Sequencing hardware: Qubit high sensitivity dsDNA assay (Thermo Fisher Scientific), Bioruptor (Diagenode), Fragment Analyzer (Agilent) and sequenced using the NovaSEQ6000 platform. Data analysis hardware: Adapter-trimmed using fastp v0.21, A de novo assembly based on Megahit v1.29 assembler, mitogenome assembly within mitoZ v3.5 pipeline. Phylogenetic analysis: IQ –TREE 2.2 was used to construct the Maximum Likelihood phylogenetic tree.. .. Data source location , The individual was rescued from Sibu, Sarawak, Malaysia, by the Sarawak Forestry Corporation (SFC) and is currently housed at Matang Wildlife Centre for rehabilitation..

DNA Extraction:

Article Title: Mitogenome data of Hylobates abbotti Kloss, 1929 (Abbott’s Gray Gibbon)
Article Snippet: .. Data collection , Buccal swab DNA sampling DNA extraction: Genomic DNA extracted using QIAamp Blood & Tissue Kit (Qiagen, Germany) Library Preparation and Sequencing hardware: Qubit high sensitivity dsDNA assay (Thermo Fisher Scientific), Bioruptor (Diagenode), Fragment Analyzer (Agilent) and sequenced using the NovaSEQ6000 platform. Data analysis hardware: Adapter-trimmed using fastp v0.21, A de novo assembly based on Megahit v1.29 assembler, mitogenome assembly within mitoZ v3.5 pipeline. Phylogenetic analysis: IQ –TREE 2.2 was used to construct the Maximum Likelihood phylogenetic tree.. .. Data source location , The individual was rescued from Sibu, Sarawak, Malaysia, by the Sarawak Forestry Corporation (SFC) and is currently housed at Matang Wildlife Centre for rehabilitation..

Tissue:

Article Title: Mitogenome data of Hylobates abbotti Kloss, 1929 (Abbott’s Gray Gibbon)
Article Snippet: .. Data collection , Buccal swab DNA sampling DNA extraction: Genomic DNA extracted using QIAamp Blood & Tissue Kit (Qiagen, Germany) Library Preparation and Sequencing hardware: Qubit high sensitivity dsDNA assay (Thermo Fisher Scientific), Bioruptor (Diagenode), Fragment Analyzer (Agilent) and sequenced using the NovaSEQ6000 platform. Data analysis hardware: Adapter-trimmed using fastp v0.21, A de novo assembly based on Megahit v1.29 assembler, mitogenome assembly within mitoZ v3.5 pipeline. Phylogenetic analysis: IQ –TREE 2.2 was used to construct the Maximum Likelihood phylogenetic tree.. .. Data source location , The individual was rescued from Sibu, Sarawak, Malaysia, by the Sarawak Forestry Corporation (SFC) and is currently housed at Matang Wildlife Centre for rehabilitation..

Sequencing:

Article Title: Mitogenome data of Hylobates abbotti Kloss, 1929 (Abbott’s Gray Gibbon)
Article Snippet: .. Data collection , Buccal swab DNA sampling DNA extraction: Genomic DNA extracted using QIAamp Blood & Tissue Kit (Qiagen, Germany) Library Preparation and Sequencing hardware: Qubit high sensitivity dsDNA assay (Thermo Fisher Scientific), Bioruptor (Diagenode), Fragment Analyzer (Agilent) and sequenced using the NovaSEQ6000 platform. Data analysis hardware: Adapter-trimmed using fastp v0.21, A de novo assembly based on Megahit v1.29 assembler, mitogenome assembly within mitoZ v3.5 pipeline. Phylogenetic analysis: IQ –TREE 2.2 was used to construct the Maximum Likelihood phylogenetic tree.. .. Data source location , The individual was rescued from Sibu, Sarawak, Malaysia, by the Sarawak Forestry Corporation (SFC) and is currently housed at Matang Wildlife Centre for rehabilitation..

Qubit:

Article Title: Mitogenome data of Hylobates abbotti Kloss, 1929 (Abbott’s Gray Gibbon)
Article Snippet: .. Data collection , Buccal swab DNA sampling DNA extraction: Genomic DNA extracted using QIAamp Blood & Tissue Kit (Qiagen, Germany) Library Preparation and Sequencing hardware: Qubit high sensitivity dsDNA assay (Thermo Fisher Scientific), Bioruptor (Diagenode), Fragment Analyzer (Agilent) and sequenced using the NovaSEQ6000 platform. Data analysis hardware: Adapter-trimmed using fastp v0.21, A de novo assembly based on Megahit v1.29 assembler, mitogenome assembly within mitoZ v3.5 pipeline. Phylogenetic analysis: IQ –TREE 2.2 was used to construct the Maximum Likelihood phylogenetic tree.. .. Data source location , The individual was rescued from Sibu, Sarawak, Malaysia, by the Sarawak Forestry Corporation (SFC) and is currently housed at Matang Wildlife Centre for rehabilitation..

Sensitivity:

Article Title: Mitogenome data of Hylobates abbotti Kloss, 1929 (Abbott’s Gray Gibbon)
Article Snippet: .. Data collection , Buccal swab DNA sampling DNA extraction: Genomic DNA extracted using QIAamp Blood & Tissue Kit (Qiagen, Germany) Library Preparation and Sequencing hardware: Qubit high sensitivity dsDNA assay (Thermo Fisher Scientific), Bioruptor (Diagenode), Fragment Analyzer (Agilent) and sequenced using the NovaSEQ6000 platform. Data analysis hardware: Adapter-trimmed using fastp v0.21, A de novo assembly based on Megahit v1.29 assembler, mitogenome assembly within mitoZ v3.5 pipeline. Phylogenetic analysis: IQ –TREE 2.2 was used to construct the Maximum Likelihood phylogenetic tree.. .. Data source location , The individual was rescued from Sibu, Sarawak, Malaysia, by the Sarawak Forestry Corporation (SFC) and is currently housed at Matang Wildlife Centre for rehabilitation..

dsDNA Assay:

Article Title: Mitogenome data of Hylobates abbotti Kloss, 1929 (Abbott’s Gray Gibbon)
Article Snippet: .. Data collection , Buccal swab DNA sampling DNA extraction: Genomic DNA extracted using QIAamp Blood & Tissue Kit (Qiagen, Germany) Library Preparation and Sequencing hardware: Qubit high sensitivity dsDNA assay (Thermo Fisher Scientific), Bioruptor (Diagenode), Fragment Analyzer (Agilent) and sequenced using the NovaSEQ6000 platform. Data analysis hardware: Adapter-trimmed using fastp v0.21, A de novo assembly based on Megahit v1.29 assembler, mitogenome assembly within mitoZ v3.5 pipeline. Phylogenetic analysis: IQ –TREE 2.2 was used to construct the Maximum Likelihood phylogenetic tree.. .. Data source location , The individual was rescued from Sibu, Sarawak, Malaysia, by the Sarawak Forestry Corporation (SFC) and is currently housed at Matang Wildlife Centre for rehabilitation..

Analysis:

Article Title: Mitogenome data of Hylobates abbotti Kloss, 1929 (Abbott’s Gray Gibbon)
Article Snippet: .. Data collection , Buccal swab DNA sampling DNA extraction: Genomic DNA extracted using QIAamp Blood & Tissue Kit (Qiagen, Germany) Library Preparation and Sequencing hardware: Qubit high sensitivity dsDNA assay (Thermo Fisher Scientific), Bioruptor (Diagenode), Fragment Analyzer (Agilent) and sequenced using the NovaSEQ6000 platform. Data analysis hardware: Adapter-trimmed using fastp v0.21, A de novo assembly based on Megahit v1.29 assembler, mitogenome assembly within mitoZ v3.5 pipeline. Phylogenetic analysis: IQ –TREE 2.2 was used to construct the Maximum Likelihood phylogenetic tree.. .. Data source location , The individual was rescued from Sibu, Sarawak, Malaysia, by the Sarawak Forestry Corporation (SFC) and is currently housed at Matang Wildlife Centre for rehabilitation..

Construct:

Article Title: Mitogenome data of Hylobates abbotti Kloss, 1929 (Abbott’s Gray Gibbon)
Article Snippet: .. Data collection , Buccal swab DNA sampling DNA extraction: Genomic DNA extracted using QIAamp Blood & Tissue Kit (Qiagen, Germany) Library Preparation and Sequencing hardware: Qubit high sensitivity dsDNA assay (Thermo Fisher Scientific), Bioruptor (Diagenode), Fragment Analyzer (Agilent) and sequenced using the NovaSEQ6000 platform. Data analysis hardware: Adapter-trimmed using fastp v0.21, A de novo assembly based on Megahit v1.29 assembler, mitogenome assembly within mitoZ v3.5 pipeline. Phylogenetic analysis: IQ –TREE 2.2 was used to construct the Maximum Likelihood phylogenetic tree.. .. Data source location , The individual was rescued from Sibu, Sarawak, Malaysia, by the Sarawak Forestry Corporation (SFC) and is currently housed at Matang Wildlife Centre for rehabilitation..

Concentration Assay:

Article Title: Enhancer profiling uncovers Jmjd1c as an essential suppressor in neuropathic pain by targeting Socs3
Article Snippet: .. Briefly, 1 × 10 7 nerve cells were cross-linked with formaldehyde at a final concentration of 1 %, and then the cells were subjected to sonication using a Diagenode Bioruptor to generate DNA fragments in the 200–500 bp size range, followed by immunoprecipitation using antibodies specific to KLF15 (Abcam, ab2647) and a control normal immunoglobulin G (IgG) (CST, 2729S). .. Briefly, 1 × 10 7 nerve cells were cross-linked with formaldehyde at a final concentration of 1 %, and then the cells were subjected to sonication using a Diagenode Bioruptor to generate DNA fragments in the 200–500 bp size range, followed by immunoprecipitation using antibodies specific to KLF15 (Abcam, ab2647) and a control normal immunoglobulin G (IgG) (CST, 2729S).

Immunoprecipitation:

Article Title: Enhancer profiling uncovers Jmjd1c as an essential suppressor in neuropathic pain by targeting Socs3
Article Snippet: .. Briefly, 1 × 10 7 nerve cells were cross-linked with formaldehyde at a final concentration of 1 %, and then the cells were subjected to sonication using a Diagenode Bioruptor to generate DNA fragments in the 200–500 bp size range, followed by immunoprecipitation using antibodies specific to KLF15 (Abcam, ab2647) and a control normal immunoglobulin G (IgG) (CST, 2729S). .. Briefly, 1 × 10 7 nerve cells were cross-linked with formaldehyde at a final concentration of 1 %, and then the cells were subjected to sonication using a Diagenode Bioruptor to generate DNA fragments in the 200–500 bp size range, followed by immunoprecipitation using antibodies specific to KLF15 (Abcam, ab2647) and a control normal immunoglobulin G (IgG) (CST, 2729S).

Control:

Article Title: Enhancer profiling uncovers Jmjd1c as an essential suppressor in neuropathic pain by targeting Socs3
Article Snippet: .. Briefly, 1 × 10 7 nerve cells were cross-linked with formaldehyde at a final concentration of 1 %, and then the cells were subjected to sonication using a Diagenode Bioruptor to generate DNA fragments in the 200–500 bp size range, followed by immunoprecipitation using antibodies specific to KLF15 (Abcam, ab2647) and a control normal immunoglobulin G (IgG) (CST, 2729S). .. Briefly, 1 × 10 7 nerve cells were cross-linked with formaldehyde at a final concentration of 1 %, and then the cells were subjected to sonication using a Diagenode Bioruptor to generate DNA fragments in the 200–500 bp size range, followed by immunoprecipitation using antibodies specific to KLF15 (Abcam, ab2647) and a control normal immunoglobulin G (IgG) (CST, 2729S).



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