buffer kits illumina (Illumina Inc)
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Illumina Inc
buffer kits illumina
Buffer Kits Illumina, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 999 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thruplex+dna-seq+12s+kit/Illumina+Tagment+DNA+Enzyme+and+Buffer+Small+Kit/pm34582804-617-236-238
Average 97 stars, based on 999 article reviews
Buffer Kits Illumina, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 999 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thruplex+dna-seq+12s+kit/Illumina+Tagment+DNA+Enzyme+and+Buffer+Small+Kit/pm34582804-617-236-238
Average 97 stars, based on 999 article reviews
buffer kits illumina - by Bioz Stars,
2026-11
97/100 stars
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ATAC-seq:Article Title: Transient SUMOylation inhibition in human pre-adipocytes stably imprints a transcriptional beiging fate Article Snippet: .. Samples for assay for transposase-accessible chromatin sequencing (ATAC-seq) were prepared based on previously published protocols with the Illumina Article Title: PUS7 pseudouridylation of 7SK orchestrates human somatic cell reprogramming into iPS cells. Article Snippet: Briefly, 100,000 human iPS cells were dissociated for each replicate and mixed with 20 μl of Drosophila nuclei spike-in (Active Motif, 53154). .. Cells were permeabilized using the buffer provided in the ATAC-seq kit (Active Motif, 53150) and accessible chromatin regions were tagmented using other:Article Title: Uncovering essential RNA components of fusion oncoprotein condensates using bicon-seq. Article Snippet: Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models dTAG-V5-NUP98::KDM5A Troester et al (2025) NA dTAG-GFP-V5-NUP98::KDM5A Troester et al (2025) NA GFP-V5-NUP98::KDM5A Zhang et al (2025) NA DDX6-dTAG MOLM13 Kodali et al (2024) NA LentiX 293T cells Takara bio 632180 U2OS ATCC HTB-96 Recombinant DNA LeGO-lnc-MONC Addgene #86966 LeGO-lnc-Gas5 This study NA pLenti-SFFV-dCas9-KRABMeCP2-P2A-Blasti Addgene #122205 psPAX2 Addgene #12260 pMD2.G Addgene #12259 Antibodies V5-Tag (D3H8Q) Rabbit mAb Cell Signaling Technology 13202S Rabbit IgG Diagenode C15410206 Mouse anti-CD11b/Mac-1 BV 421 (clone M1/70) BioLegend 101235 Mouse anti-Gr-1/Ly-6C PE-Cy7 (clone RB6-8C5) BioLegend 108415 Mouse anti-CD117/c-Kit APC (clone 2B8) BioLegend 105811 Anti-GFP Abcam ab290 Normal Rabbit IgG Cell Signaling Technology 2729 Rabbit anti-DDX6 Novus Biologicals NB200-192 Mouse anti-G3BP1 (F6X8V) Cell Signaling Technology 48372 Rabbit anti-PSPC1 (E7L8B) Cell Signaling Technology 65992 Rabbit anti-NPM1 Abcam ab15440 Rabbit anti-Coilin Abcam ab210785 Rabbit anti-TRAP220/MED1 Abcam ab64965 F(ab’)2-Goat anti-Rabbit IgG (H+ L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen A-21246 14 The EMBO Journal © The Author(s) Reagent/Resource Reference or Source Identifier or Catalog Number Goat anti-Mouse IgG (H+ L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen A-21235 Oligonucleotides and other sequence-based reagents PCR primers This study Dataset EV1 Chemicals, enzymes and other reagents Biotinylated isoxazole Sigma-Aldrich 900572 7-AAD Viability Staining Solution Invitrogen 00-6993-42 Annexin V Pacific Blue conjugate Invitrogen #A35122 Sodium arsenite Sigma-Aldrich S7400 DMSO Sigma-Aldrich D4540 dTAG-13 Sigma-Aldrich SML2601 DMEM, high glucose, no glutamine Gibco, Thermo Fisher Scientific 11510416 IMDM Gibco, Thermo Fisher Scientific 11504556 FBS Sigma-Aldrich F9665 Penicillin-Streptomycin Gibco, Thermo Fisher Scientific 11548876 L-Glutamine Gibco, Thermo Fisher Scientific 11500626 2-Mercaptoethanol Gibco, Fisher Scientific 11528926 SCF PeproTech, Thermo Fisher Scientific 250-03-10UG mIL-3 PeproTech, Thermo Fisher Scientific 213-13-10UG mIL-6 PeproTech, Thermo Fisher Scientific 216-16-10UG RPMI 1640 Gibco, Thermo Fisher Scientific 11544446 HEPES Sigma-Aldrich H0887 Sodium pyruvate Sigma-Aldrich S8636 DMEM, high glucose, GlutaMAX Supplement Gibco, Thermo Fisher Scientific 10566016 Polybrene Szabo-Scandic SACSC134220A 0.45 μM filter TPP TPP-99745 NaCl Carl Roth HN00.3 NP-40 alternative Millipore 492016 EDTA Sigma-Aldrich E9884 EGTA Millipore 324626 Glycerol Thermo Scientific Chemicals 158920025 Protease Inhibitor Cocktail Roche 11697498001 NaF Sigma-Aldrich 201154 PMSF Roche 10837091001 DTT Thermo Scientific A39255 TPCK Sigma-Aldrich T4376 Reagent/Resource Reference or Source Identifier or Catalog Number Pierce Bradford Protein Assay Kit Thermo Scientific 23200 NEB Monarch Total RNA Miniprep Kit NEB T1110 QuantSeq 3‘ mRNA-Seq Library Prep Kit Lexogen 015.96 Tris-HCl ITW reagents,AppliChem A1087,1000 MgCl2 Sigma-Aldrich M8266 Vanadyl Ribonucleoside Complex NEB S1402S RNA ChIP-IT Magnetic Chromatin Immunoprecipitation Kit Active Motif Cat#53024 Formaldehyde Sigma-Aldrich 252549 Trizol LS Reagent Invitrogen 10296010 Chloroform Merck 102447 Isopropanol Merck 190764 Linear acrylamide Thermo Scientific Chemicals J67830-XF RevertAid First Strand cDNA Synthesis Kit Thermo Scientific K1621 SsoAdvanced Universal SYBR Green Supermix Bio-Rad #1725271 RNAscope Multiplex Fluorescent v2 Assay ACD Bio-Techne 323100 Histofix Roth P087.6 Ethanol Sigma-Aldrich 51976 RNAscope probe-Mm-Gas5 Tocris Bioscience #561631 RNAscope probe-Mm-Malat1 Tocris Bioscience #313391 RNAscope negative control probe Tocris Bioscience #320871 TSA-Cy3 Tocris Bioscience #7527 Polyethylenimine Merck 408727 Spermidine Sigma-Aldrich S2626 Digitonin Sigma-Aldrich D141 BioMagPlus Concanavalin A Polysciences 86057 RNase A (DNase-free) ITW Reagents Lysis:Article Title: Long-term immunoprophylaxis by TLR9 ligand mediates macrophage-ILC cross-talk against respiratory viral infection Article Snippet: .. Cells were lysed in cold lysis buffer [10 mM tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl 2 , and 0.1% IGEPAL CA-630] on ice for 10 min. After centrifugation, nuclei pellets were resuspended with 50 μl of transposase reaction mix [25 μl of Tagment DNA buffer (Illumina), 2.5 μl of Centrifugation:Article Title: Long-term immunoprophylaxis by TLR9 ligand mediates macrophage-ILC cross-talk against respiratory viral infection Article Snippet: .. Cells were lysed in cold lysis buffer [10 mM tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl 2 , and 0.1% IGEPAL CA-630] on ice for 10 min. After centrifugation, nuclei pellets were resuspended with 50 μl of transposase reaction mix [25 μl of Tagment DNA buffer (Illumina), 2.5 μl of Incubation:Article Title: Long-term immunoprophylaxis by TLR9 ligand mediates macrophage-ILC cross-talk against respiratory viral infection Article Snippet: .. Cells were lysed in cold lysis buffer [10 mM tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl 2 , and 0.1% IGEPAL CA-630] on ice for 10 min. After centrifugation, nuclei pellets were resuspended with 50 μl of transposase reaction mix [25 μl of Tagment DNA buffer (Illumina), 2.5 μl of Article Title: Transient SUMOylation inhibition in human pre-adipocytes stably imprints a transcriptional beiging fate Article Snippet: .. The supernatant was removed and the nuclei pellet was resuspended in 50 μl transposition mixture [25 μl Tagment DNA Buffer (1× final, Illumina, 20034197), 2.5 μl Purification:Article Title: Long-term immunoprophylaxis by TLR9 ligand mediates macrophage-ILC cross-talk against respiratory viral infection Article Snippet: .. Cells were lysed in cold lysis buffer [10 mM tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl 2 , and 0.1% IGEPAL CA-630] on ice for 10 min. After centrifugation, nuclei pellets were resuspended with 50 μl of transposase reaction mix [25 μl of Tagment DNA buffer (Illumina), 2.5 μl of Polymerase Chain Reaction:Article Title: Long-term immunoprophylaxis by TLR9 ligand mediates macrophage-ILC cross-talk against respiratory viral infection Article Snippet: .. Cells were lysed in cold lysis buffer [10 mM tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl 2 , and 0.1% IGEPAL CA-630] on ice for 10 min. After centrifugation, nuclei pellets were resuspended with 50 μl of transposase reaction mix [25 μl of Tagment DNA buffer (Illumina), 2.5 μl of |