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nebnext rrna depletion kit  (New England Biolabs)


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    Structured Review

    New England Biolabs nebnext rrna depletion kit
    Nebnext Rrna Depletion Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 924 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thruplex+dna-seq+12s+kit/NEBNext+rRNA+Depletion+Kit/custom%40e6310%4027393904
    Average 97 stars, based on 924 article reviews
    nebnext rrna depletion kit - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Immunoprecipitation:

    Article Title: The RNA m 6 A landscape during human oocyte-to-embryo transition.
    Article Snippet: .. Reagent/resource Reference or source Identifier or catalog number Tris-HCl pH 7.5 solution Life Technologies Cat #: 15567-027 Tris-HCl pH 8.0 solution Life Technologies Cat #: 15568025 NaCl solution Sigma-Aldrich Cat #: 71386 NP-40 Thermofisher Scientific Cat #: 85124 RiboLock RNase inhibitor Thermofisher Scientific Cat #: EO0381 NaCl solution Sigma-Aldrich Cat #: 71386 Triton X-100 Merck Life Sciences Cat #: T878750ML SDS solution Thermofisher Scientific Cat #: 11538896 EDTA pH 8 solution BioWORLD Cat #: 40520000 EGTA pH 8 solution BioWORLD Cat #: 50-255- 956 Sodium deoxycholate, 10% solution BioWORLD Cat #: 40430018-1 Proteinase K New England Biolabs Cat #: P8107S 3M sodium acetate, pH 5.2 Thermofisher Scientific Cat #: R1181 Linear acrylamide Thermofisher Scientific Cat #: AM9520 SMART-Seq® Stranded Kit Takara Cat #: 634442 STM2457 MCE Cat #: HY134836 DynabeadsTM Protein A for Immunoprecipitation Thermofisher Scientific Cat #: 10001D NEBNext rRNA depletion kit New England Biolabs Cat #: E6310L Hijack the library quantification kit Roche Cat #: kk4824 High Sensitivity D1000 ScreenTape Agilent Cat #: 5067- 5584 Software FastQC (v0.11.8) https://www.bioinformatics. babraham.ac.uk/projects/ fastqc/ Cutadapt (v1.8.1) EMBnetjournal (Marcel, 2011) HISAT2 (v2.1.0) Nat Methods (Kim et al, 2015) SAMtools (v1.9) Bioinformatics (Li et al, 2009) BEDTools (v2.28.0) Bioinformatics (Quinlan and Hall, 2010) StringTie (v1.3.5) Nat Biotechnol (Pertea et al, 2015) NormExpression (v0.1.0) Front Genet (Wu et al, 2019) deepTools (v3.2.0) Nucleic Acids Res (Ramirez et al, 2014) MACS2 (v2.1.2) Genome Biol (Zhang et al, 2008) MetaPlotR Bioinformatics (OlarerinGeorge and Jaffrey, 2017) Homer (v4.11.1) Mol Cell (Heinz et al, 2010) Reagent/resource Reference or source Identifier or catalog number BioQC (v1.10.0) BMC Genomics (Zhang et al, 2017) DAVID (v6.8) Nat Protoc (Huang et al, 2009) Other Illumina NovaSeq sequencing Illumina Paired-end mode ..

    Library Quantification:

    Article Title: The RNA m 6 A landscape during human oocyte-to-embryo transition.
    Article Snippet: .. Reagent/resource Reference or source Identifier or catalog number Tris-HCl pH 7.5 solution Life Technologies Cat #: 15567-027 Tris-HCl pH 8.0 solution Life Technologies Cat #: 15568025 NaCl solution Sigma-Aldrich Cat #: 71386 NP-40 Thermofisher Scientific Cat #: 85124 RiboLock RNase inhibitor Thermofisher Scientific Cat #: EO0381 NaCl solution Sigma-Aldrich Cat #: 71386 Triton X-100 Merck Life Sciences Cat #: T878750ML SDS solution Thermofisher Scientific Cat #: 11538896 EDTA pH 8 solution BioWORLD Cat #: 40520000 EGTA pH 8 solution BioWORLD Cat #: 50-255- 956 Sodium deoxycholate, 10% solution BioWORLD Cat #: 40430018-1 Proteinase K New England Biolabs Cat #: P8107S 3M sodium acetate, pH 5.2 Thermofisher Scientific Cat #: R1181 Linear acrylamide Thermofisher Scientific Cat #: AM9520 SMART-Seq® Stranded Kit Takara Cat #: 634442 STM2457 MCE Cat #: HY134836 DynabeadsTM Protein A for Immunoprecipitation Thermofisher Scientific Cat #: 10001D NEBNext rRNA depletion kit New England Biolabs Cat #: E6310L Hijack the library quantification kit Roche Cat #: kk4824 High Sensitivity D1000 ScreenTape Agilent Cat #: 5067- 5584 Software FastQC (v0.11.8) https://www.bioinformatics. babraham.ac.uk/projects/ fastqc/ Cutadapt (v1.8.1) EMBnetjournal (Marcel, 2011) HISAT2 (v2.1.0) Nat Methods (Kim et al, 2015) SAMtools (v1.9) Bioinformatics (Li et al, 2009) BEDTools (v2.28.0) Bioinformatics (Quinlan and Hall, 2010) StringTie (v1.3.5) Nat Biotechnol (Pertea et al, 2015) NormExpression (v0.1.0) Front Genet (Wu et al, 2019) deepTools (v3.2.0) Nucleic Acids Res (Ramirez et al, 2014) MACS2 (v2.1.2) Genome Biol (Zhang et al, 2008) MetaPlotR Bioinformatics (OlarerinGeorge and Jaffrey, 2017) Homer (v4.11.1) Mol Cell (Heinz et al, 2010) Reagent/resource Reference or source Identifier or catalog number BioQC (v1.10.0) BMC Genomics (Zhang et al, 2017) DAVID (v6.8) Nat Protoc (Huang et al, 2009) Other Illumina NovaSeq sequencing Illumina Paired-end mode ..

    Software:

    Article Title: The RNA m 6 A landscape during human oocyte-to-embryo transition.
    Article Snippet: .. Reagent/resource Reference or source Identifier or catalog number Tris-HCl pH 7.5 solution Life Technologies Cat #: 15567-027 Tris-HCl pH 8.0 solution Life Technologies Cat #: 15568025 NaCl solution Sigma-Aldrich Cat #: 71386 NP-40 Thermofisher Scientific Cat #: 85124 RiboLock RNase inhibitor Thermofisher Scientific Cat #: EO0381 NaCl solution Sigma-Aldrich Cat #: 71386 Triton X-100 Merck Life Sciences Cat #: T878750ML SDS solution Thermofisher Scientific Cat #: 11538896 EDTA pH 8 solution BioWORLD Cat #: 40520000 EGTA pH 8 solution BioWORLD Cat #: 50-255- 956 Sodium deoxycholate, 10% solution BioWORLD Cat #: 40430018-1 Proteinase K New England Biolabs Cat #: P8107S 3M sodium acetate, pH 5.2 Thermofisher Scientific Cat #: R1181 Linear acrylamide Thermofisher Scientific Cat #: AM9520 SMART-Seq® Stranded Kit Takara Cat #: 634442 STM2457 MCE Cat #: HY134836 DynabeadsTM Protein A for Immunoprecipitation Thermofisher Scientific Cat #: 10001D NEBNext rRNA depletion kit New England Biolabs Cat #: E6310L Hijack the library quantification kit Roche Cat #: kk4824 High Sensitivity D1000 ScreenTape Agilent Cat #: 5067- 5584 Software FastQC (v0.11.8) https://www.bioinformatics. babraham.ac.uk/projects/ fastqc/ Cutadapt (v1.8.1) EMBnetjournal (Marcel, 2011) HISAT2 (v2.1.0) Nat Methods (Kim et al, 2015) SAMtools (v1.9) Bioinformatics (Li et al, 2009) BEDTools (v2.28.0) Bioinformatics (Quinlan and Hall, 2010) StringTie (v1.3.5) Nat Biotechnol (Pertea et al, 2015) NormExpression (v0.1.0) Front Genet (Wu et al, 2019) deepTools (v3.2.0) Nucleic Acids Res (Ramirez et al, 2014) MACS2 (v2.1.2) Genome Biol (Zhang et al, 2008) MetaPlotR Bioinformatics (OlarerinGeorge and Jaffrey, 2017) Homer (v4.11.1) Mol Cell (Heinz et al, 2010) Reagent/resource Reference or source Identifier or catalog number BioQC (v1.10.0) BMC Genomics (Zhang et al, 2017) DAVID (v6.8) Nat Protoc (Huang et al, 2009) Other Illumina NovaSeq sequencing Illumina Paired-end mode ..

    Sequencing:

    Article Title: The RNA m 6 A landscape during human oocyte-to-embryo transition.
    Article Snippet: .. Reagent/resource Reference or source Identifier or catalog number Tris-HCl pH 7.5 solution Life Technologies Cat #: 15567-027 Tris-HCl pH 8.0 solution Life Technologies Cat #: 15568025 NaCl solution Sigma-Aldrich Cat #: 71386 NP-40 Thermofisher Scientific Cat #: 85124 RiboLock RNase inhibitor Thermofisher Scientific Cat #: EO0381 NaCl solution Sigma-Aldrich Cat #: 71386 Triton X-100 Merck Life Sciences Cat #: T878750ML SDS solution Thermofisher Scientific Cat #: 11538896 EDTA pH 8 solution BioWORLD Cat #: 40520000 EGTA pH 8 solution BioWORLD Cat #: 50-255- 956 Sodium deoxycholate, 10% solution BioWORLD Cat #: 40430018-1 Proteinase K New England Biolabs Cat #: P8107S 3M sodium acetate, pH 5.2 Thermofisher Scientific Cat #: R1181 Linear acrylamide Thermofisher Scientific Cat #: AM9520 SMART-Seq® Stranded Kit Takara Cat #: 634442 STM2457 MCE Cat #: HY134836 DynabeadsTM Protein A for Immunoprecipitation Thermofisher Scientific Cat #: 10001D NEBNext rRNA depletion kit New England Biolabs Cat #: E6310L Hijack the library quantification kit Roche Cat #: kk4824 High Sensitivity D1000 ScreenTape Agilent Cat #: 5067- 5584 Software FastQC (v0.11.8) https://www.bioinformatics. babraham.ac.uk/projects/ fastqc/ Cutadapt (v1.8.1) EMBnetjournal (Marcel, 2011) HISAT2 (v2.1.0) Nat Methods (Kim et al, 2015) SAMtools (v1.9) Bioinformatics (Li et al, 2009) BEDTools (v2.28.0) Bioinformatics (Quinlan and Hall, 2010) StringTie (v1.3.5) Nat Biotechnol (Pertea et al, 2015) NormExpression (v0.1.0) Front Genet (Wu et al, 2019) deepTools (v3.2.0) Nucleic Acids Res (Ramirez et al, 2014) MACS2 (v2.1.2) Genome Biol (Zhang et al, 2008) MetaPlotR Bioinformatics (OlarerinGeorge and Jaffrey, 2017) Homer (v4.11.1) Mol Cell (Heinz et al, 2010) Reagent/resource Reference or source Identifier or catalog number BioQC (v1.10.0) BMC Genomics (Zhang et al, 2017) DAVID (v6.8) Nat Protoc (Huang et al, 2009) Other Illumina NovaSeq sequencing Illumina Paired-end mode ..

    other:

    Article Title: Expression of an interleukin-2 partial agonist enhances regulatory T cell persistence and efficacy in mouse autoimmune models
    Article Snippet: RNA library preparation were realized following manufacturer’s recommendations (NEBnext rRNA depletion #E6310 + Ultra II Directional RNA kit #E7760L from New England Biolabs).

    Article Title: Sex-dimorphic gene regulation in murine macrophages across niches.
    Article Snippet: Library quality was assessed using the 4200 TapeStation system (Agilent Technologies) with a High Sensitivity DNA ScreenTape 1000 (Agilent, no. 5067-5584).



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