anti mouse sca1 microbead kit (Miltenyi Biotec)

Miltenyi Biotec is a verified supplier

Miltenyi Biotec manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Miltenyi Biotec anti mouse sca1 microbead kit
$FACS Plots Showing Lin − <t>Sca1</t> + c-Kit + (LSK) Cell Fractions after MACS Purification and 2 Days of Culture Bone marrow cells were stained with α-mouse lineage cocktail, Sca1, and c-Kit antibodies. (A) The negative fraction of the LS column should be almost completely depleted of Sca1 + cells. (B) The positive fraction eluted from the LS or MS separation column is enriched in LSK cells and progenitor cells, which all express Sca1. The LSK population should comprise 6 to 8% of the Sca1 + cell fraction. Lymphoid progenitor cells require IL-7 to survive and will be depleted upon culturing with 50 ng/mL mTPO, mSCF, mFLT-3, and hIL-11. (C) Purified LSK cell fraction after 2 days of culture in HSPC medium.$
Anti Mouse Sca1 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thruplex+dna+seq+6s+12+kit/Anti-Sca-1+MicroBead+Kit+(Vio+Bright+FITC)%2C+mouse/pmc07357675-65-0-7
Average 93 stars, based on 10 article reviews

anti mouse sca1 microbead kit - by Bioz Stars, 2026-11

93/100 stars

Images

1) Product Images from "Protocol for Efficient CRISPR/Cas9/AAV-Mediated Homologous Recombination in Mouse Hematopoietic Stem and Progenitor Cells"

Article Title: Protocol for Efficient CRISPR/Cas9/AAV-Mediated Homologous Recombination in Mouse Hematopoietic Stem and Progenitor Cells

Journal: STAR Protocols

doi: 10.1016/j.xpro.2020.100028

$FACS Plots Showing Lin − Sca1 + c-Kit + (LSK) Cell Fractions after MACS Purification and 2 Days of Culture Bone marrow cells were stained with α-mouse lineage cocktail, Sca1, and c-Kit antibodies. (A) The negative fraction of the LS column should be almost completely depleted of Sca1 + cells. (B) The positive fraction eluted from the LS or MS separation column is enriched in LSK cells and progenitor cells, which all express Sca1. The LSK population should comprise 6 to 8% of the Sca1 + cell fraction. Lymphoid progenitor cells require IL-7 to survive and will be depleted upon culturing with 50 ng/mL mTPO, mSCF, mFLT-3, and hIL-11. (C) Purified LSK cell fraction after 2 days of culture in HSPC medium.$
Figure Legend Snippet: FACS Plots Showing Lin − Sca1 + c-Kit + (LSK) Cell Fractions after MACS Purification and 2 Days of Culture Bone marrow cells were stained with α-mouse lineage cocktail, Sca1, and c-Kit antibodies. (A) The negative fraction of the LS column should be almost completely depleted of Sca1 + cells. (B) The positive fraction eluted from the LS or MS separation column is enriched in LSK cells and progenitor cells, which all express Sca1. The LSK population should comprise 6 to 8% of the Sca1 + cell fraction. Lymphoid progenitor cells require IL-7 to survive and will be depleted upon culturing with 50 ng/mL mTPO, mSCF, mFLT-3, and hIL-11. (C) Purified LSK cell fraction after 2 days of culture in HSPC medium.

Techniques Used: Purification, Staining

Figure Legend Snippet:

Techniques Used: Virus, Recombinant, DNA Extraction, Cloning, Modification, Plasmid Preparation, Software, Adhesive, Real-time Polymerase Chain Reaction, Sterility, Centrifugation

Lysis:

Article Title: Neonatal gene therapy effectively prevents disease manifestations in a murine model of Mucopolysaccharidosis type I.
Article Snippet: .. After lysis with ammonium chloride (ACK) lysing buffer (STEMCELL Technologies, Vancouver, British Columbia, Canada), BM cell populations were enriched in progenitor cells using the anti-Sca-1 MicroBead Kit (Miltenyi Biotech, Bergisch Gladbach, Germany), according to the manufacturer’s instructions. .. The enriched cells were cultured at the concentration of 1 × 106 cells/mL in StemSpan SFEM (STEMCELL Technologies) supplemented with 1% glutamine, 1% Pen/Strep, 100 ng/ μL human Fms-related tyrosine kinase 3 ligand (hFlt3-L), 100 ng/ μL murine stem cell factor, 50 ng/μL murine thromobopoietin (TPO), and 20 ng/μL human interleukin 3 (Peprotech, Cranbury, NJ, USA).

Article Title: Neonatal gene therapy effectively prevents disease manifestations in a murine model of Mucopolysaccharidosis type I
Article Snippet: .. After lysis with ammonium chloride (ACK) lysing buffer (STEMCELL Technologies, Vancouver, British Columbia, Canada), BM cell populations were enriched in progenitor cells using the anti-Sca-1 MicroBead Kit (Miltenyi Biotech, Bergisch Gladbach, Germany), according to the manufacturer’s instructions. .. The enriched cells were cultured at the concentration of 1 × 10 6 cells/mL in StemSpan SFEM (STEMCELL Technologies) supplemented with 1% glutamine, 1% Pen/Strep, 100 ng/μL human Fms-related tyrosine kinase 3 ligand (hFlt3-L), 100 ng/μL murine stem cell factor, 50 ng/μL murine thromobopoietin (TPO), and 20 ng/μL human interleukin 3 (Peprotech, Cranbury, NJ, USA).

other:

Article Title: Transplantation of Wild-Type Hematopoietic Stem and Progenitor Cells Improves Disease Phenotypes in a Mucopolysaccharidosis IIIC Mouse Model.
Article Snippet: HSPCs were isolated by immunomagnetic separation using an anti-Sca1+ antibody conjugated to magnetic beads (130-123-124, Miltenyi Biotec, Germany).

Isolation:

Article Title: Identification of Critical Molecular Pathways Induced by HDAC11 Overexpression in Cardiac Mesenchymal Stem Cells
Article Snippet: .. Lin − Sca-1 + cells were subsequently isolated from the cardiac-derived cells using a hematopoietic Lin-depletion cocktail (StemCell Technologies, Vancouver, BC, Canada) followed by magnetic sorting with an anti-Sca-1-microbead kit (Miltenyi Biotec), following the manufacturer’s instructions. ..

Article Title: Identification of Critical Molecular Pathways Induced by HDAC11 Overexpression in Cardiac Mesenchymal Stem Cells.
Article Snippet: .. Lin−Sca-1+ cells were subsequently isolated from the cardiac-derived cells using a hematopoietic Lindepletion cocktail (StemCell Technologies, Vancouver, BC, Canada) followed by magnetic sorting with an anti-Sca-1-microbead kit (Miltenyi Biotec), following the manufacturer’s instructions. ..

Review