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protein thermal shift buffer  (Thermo Fisher)


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    Thermo Fisher protein thermal shift buffer
    Protein Thermal Shift Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thermal+shift+buffer/pmc11744305-82-4-23
    Average 90 stars, based on 1 article reviews
    protein thermal shift buffer - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: The novel target, mechanism and agonist of α-Ketoglutaric acid in delaying mesenchymal stem cell senescence
    Article Snippet: IDH1 activity assay To detect the direct effect of Scu on the IDH1 protein in MSCs, IDH1 antibody plates were generated by coating IDH1 antibody (Proteintech, Wuhan, China) onto 96-well plates (100 ng/well) overnight at 4°C.

    Purification:

    Article Title: Characterization of p38α autophosphorylation inhibitors that target the non-canonical activation pathway.
    Article Snippet: .. For purified proteins, sampleswere prepared in triplicates bymixing in MultiPlate 96-well white plates purified GST-p38α or GST proteins (1.5μM) with assay buffer (20mM TRIS pH 7.5, 100mM NaCl, 2mM MgCl2, 2mM DTT), Protein Thermal Shift buffer (1X final concentration, Applied Biosystem Protein Thermal ShiftTM Kit) and NC-p38i compounds at different concentrations (10mM stock in 100% DMSO) or the equivalent volume of DMSO as a control. .. After 30min incubation at RT, 1X SYPROOrangewas added and fluorescence intensity was measured at 1 °C intervals from 25 to 95 °C at a rate of 0.5 °C/s (CFX96, BioRad).

    Concentration Assay:

    Article Title: Characterization of p38α autophosphorylation inhibitors that target the non-canonical activation pathway.
    Article Snippet: .. For purified proteins, sampleswere prepared in triplicates bymixing in MultiPlate 96-well white plates purified GST-p38α or GST proteins (1.5μM) with assay buffer (20mM TRIS pH 7.5, 100mM NaCl, 2mM MgCl2, 2mM DTT), Protein Thermal Shift buffer (1X final concentration, Applied Biosystem Protein Thermal ShiftTM Kit) and NC-p38i compounds at different concentrations (10mM stock in 100% DMSO) or the equivalent volume of DMSO as a control. .. After 30min incubation at RT, 1X SYPROOrangewas added and fluorescence intensity was measured at 1 °C intervals from 25 to 95 °C at a rate of 0.5 °C/s (CFX96, BioRad).

    Control:

    Article Title: Characterization of p38α autophosphorylation inhibitors that target the non-canonical activation pathway.
    Article Snippet: .. For purified proteins, sampleswere prepared in triplicates bymixing in MultiPlate 96-well white plates purified GST-p38α or GST proteins (1.5μM) with assay buffer (20mM TRIS pH 7.5, 100mM NaCl, 2mM MgCl2, 2mM DTT), Protein Thermal Shift buffer (1X final concentration, Applied Biosystem Protein Thermal ShiftTM Kit) and NC-p38i compounds at different concentrations (10mM stock in 100% DMSO) or the equivalent volume of DMSO as a control. .. After 30min incubation at RT, 1X SYPROOrangewas added and fluorescence intensity was measured at 1 °C intervals from 25 to 95 °C at a rate of 0.5 °C/s (CFX96, BioRad).

    Article Title: 19 F chemical library and 19 F-NMR for a weakly bound complex structure.
    Article Snippet: .. For the no protein control (NPC) sample, 5 μL of protein thermal shift buffer (ABI), 2.5 μL of 1000×-diluted protein thermal shiftTM dye (ABI), and 12.5 μL of solvent, which was used for dissolving FKBP12 in the other samples, were mixed. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Discovery of a common light chain bispecific antibody targeting PD-1 and PD-L1 by Hybridoma-to-Phage-to-Yeast (H2PtY) platform
    Article Snippet: The melting temperature (Tm) values of the candidates were measured with a Protein Thermal Shift Dye Kit (Thermo Fisher Scientific, USA). .. Samples were mixed with Protein Thermal Shift buffer and Protein Thermal Shift dye solution, and then, protein melt curves were assessed in an Applied Biosystems Real-Time PCR System (QuantStudio 3, Thermo Fisher Scientific, USA). ..

    Solvent:

    Article Title: 19 F chemical library and 19 F-NMR for a weakly bound complex structure.
    Article Snippet: .. For the no protein control (NPC) sample, 5 μL of protein thermal shift buffer (ABI), 2.5 μL of 1000×-diluted protein thermal shiftTM dye (ABI), and 12.5 μL of solvent, which was used for dissolving FKBP12 in the other samples, were mixed. ..

    Adhesive:

    Article Title: Glycoside Hydrolase Catalysis: Do Substrates and Mechanism-Based Covalent Inhibitors React via Matching Transition States?
    Article Snippet: Reactions were performed in MicroAmp 96-well optical PCR plates (Applied Biosystems) at a final volume of 20 μL per well. .. Each reaction contained between 0.3 and 0.5 mg/mL protein, 1× Protein Thermal Shift Dye (Thermo Fisher Scientific) in 1× Protein Thermal Shift Buffer (Thermo Fisher Scientific) and sealed with MicroAmp Optical Adhesive Film (Thermo Fisher Scientific). ..



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    Protein Thermal Shift assay data documenting the β-clamp thermal stabilisation induced by a subset of the yBRET-identified hit compounds and their analogues. ΔTmB (i.e. ΔTm calculated using the Boltzmann method) is reported as average (with SD indicated by error bars) of 4 replicates; black dots represent individual data points. Analogues of hit compounds 11 and 19 are shown as purple and green bars, respectively (see Tables S3 and S4 for details). Reference compound 5 is represented by the blue bar; no significant thermal stabilisation (PTS) effect was detected for hit compound 7 and its analogues.

    Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

    Article Title: Small molecules targeting the eubacterial β-sliding clamp discovered by combined in silico and in vitro screening approaches

    doi: 10.1080/14756366.2024.2440861

    Figure Lengend Snippet: Protein Thermal Shift assay data documenting the β-clamp thermal stabilisation induced by a subset of the yBRET-identified hit compounds and their analogues. ΔTmB (i.e. ΔTm calculated using the Boltzmann method) is reported as average (with SD indicated by error bars) of 4 replicates; black dots represent individual data points. Analogues of hit compounds 11 and 19 are shown as purple and green bars, respectively (see Tables S3 and S4 for details). Reference compound 5 is represented by the blue bar; no significant thermal stabilisation (PTS) effect was detected for hit compound 7 and its analogues.

    Article Snippet: Protein Thermal Shift TM Buffer (ThermoFisher; 5 μL) was added to each well of a RP, LF, SEMI Sk, 96 well plate (0.2 ml) (#AB19700, BIOplastics).

    Techniques: Thermal Shift Assay, Analogues