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customized microarray chip  (Agilent technologies)


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    Structured Review

    Agilent technologies customized microarray chip
    Customized Microarray Chip, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/the+customized+microarray+chips/pm38158220-248-32-41
    Average 90 stars, based on 1 article reviews
    customized microarray chip - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: The highly polymorphic CYP6M7 cytochrome P450 gene partners with the directionally selected CYP6P9a and CYP6P9b genes to expand the pyrethroid resistance front in the malaria vector Anopheles funestus in Africa.
    Article Snippet: .. To detect the genes associated with the expansion of the pyrethroid resistance front in An. funestus, a new 8×60 k Agilent microarray chip was designed using the eArray program (Agilent) (A-MEXP-2374) by adding the 15,527 Expressed Sequence Tags (ESTs) generated from another transcriptome sequencing of An. funestus [16] to the previous 4×44 k array used by [4]. ..

    Article Title: Artemisinin resistance in the malaria parasite, Plasmodium falciparum, originates from its initial transcriptional response.
    Article Snippet: The amplified product was purified using Ampure XP magnetic beads (Beckman Coulter) on Microlab Nimbus robotic platform (Hamilton) and 100 ng of cDNA was used for subsequent 10 rounds of amplification to generate aminoallyl-coupled cDNA for the hybridizations74,75. .. 17ul (~5 μg) of each Cy-5labelled (GE Healthcare) cDNA of patient’s sample and an equal amount of Cy-3labelled (GE Healthcare) cDNA of the reference pool were then hybridized together on our customized microarray chip using commercially available hybridization platform (Agilent) for 20 h at 70 °C with rotation at 10 rpm. .. Microarrays were washed and immediately scanned using Power Scanner (Tecan) at 10 μM resolution and with automated photomultiplier tubes gain adjustments to balance the signal intensities between both channels.

    Article Title: Phenotypic and microarray analysis reveals salinity stress-induced oxidative tolerance in transgenic rice expressing a DEAD-box RNA helicase, OsDB10.
    Article Snippet: Helicases are the motor proteins not only involved in the process of mRNA metabolism but also played a significant role in providing abiotic stresses tolerance.. In this study, a DEAD-box RNA helicase OsDB10 was cloned and functionally characterized.. The transcript levels of OsDB10 were increased both in shoot and root upon salt, heat, cold, and ABA application and was more prominent in shoot compared to root.

    Article Title: Extracellular vesicles from bone marrow mesenchymal stem cells alleviate osteoporosis in mice through USP7-mediated YAP1 protein stability and the Wnt/β-catenin pathway.
    Article Snippet: Mesenchymal stem cells (MSCs) and their derived extracellular vesicles (EVs) have emerged as promising tools for promoting bone regeneration.. This study investigates the functions of EVs derived from bone marrow-derived MSCs (BMSCs) in osteoporosis (OP) and the molecular mechanism.. EVs were isolated from primary BMSCs in mice.

    Article Title: Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model.
    Article Snippet: .. 17 μl (~5 μg) of each Cy5-labeled (GE Healthcare) cDNA of the sample and an equal amount of Cy3labeled (GE Healthcare) cDNA of the reference pool were then hybridized together on a customized microarray chip using a commercially available hybridization platform (Agilent) for 20 h at 70°C with gentle rotation. .. Microarrays were washed and immediately scanned using Power Scanner (Tecan) at 10-μm resolution and with automated photomultiplier tubes gain adjustments to balance the signal intensities between both channels.

    Article Title: An integrative bioinformatics approach to decipher adipocyte-induced transdifferentiation of osteoblast.
    Article Snippet: Actively secreted proteins were selected based on their association with the “extracellular matrix” (GO:0031012) or “extracellular space” (GO:0005615) gene ontology (GO) terms from NCBI and Ensembl GO annotations. .. Transcriptional changes were previously monitored in 7 biological replicates, each consisting of osteoblasts grown alone and cocultured osteoblasts after 9 h or 48 h, using the Agilent Human SurePrint G3 Microarray, 8 × 60 K microarray chip (Agilent Technologies) [7]. ..

    Article Title: Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model
    Article Snippet: .. 17 μl (∼5 μg) of each Cy5-labeled (GE Healthcare) cDNA of the sample and an equal amount of Cy3-labeled (GE Healthcare) cDNA of the reference pool were then hybridized together on a customized microarray chip using a commercially available hybridization platform (Agilent) for 20 h at 70°C with gentle rotation. .. Microarrays were washed and immediately scanned using Power Scanner (Tecan) at 10-μm resolution and with automated photomultiplier tubes gain adjustments to balance the signal intensities between both channels.

    Sequencing:

    Article Title: The highly polymorphic CYP6M7 cytochrome P450 gene partners with the directionally selected CYP6P9a and CYP6P9b genes to expand the pyrethroid resistance front in the malaria vector Anopheles funestus in Africa.
    Article Snippet: .. To detect the genes associated with the expansion of the pyrethroid resistance front in An. funestus, a new 8×60 k Agilent microarray chip was designed using the eArray program (Agilent) (A-MEXP-2374) by adding the 15,527 Expressed Sequence Tags (ESTs) generated from another transcriptome sequencing of An. funestus [16] to the previous 4×44 k array used by [4]. ..

    Generated:

    Article Title: The highly polymorphic CYP6M7 cytochrome P450 gene partners with the directionally selected CYP6P9a and CYP6P9b genes to expand the pyrethroid resistance front in the malaria vector Anopheles funestus in Africa.
    Article Snippet: .. To detect the genes associated with the expansion of the pyrethroid resistance front in An. funestus, a new 8×60 k Agilent microarray chip was designed using the eArray program (Agilent) (A-MEXP-2374) by adding the 15,527 Expressed Sequence Tags (ESTs) generated from another transcriptome sequencing of An. funestus [16] to the previous 4×44 k array used by [4]. ..

    Hybridization:

    Article Title: Artemisinin resistance in the malaria parasite, Plasmodium falciparum, originates from its initial transcriptional response.
    Article Snippet: The amplified product was purified using Ampure XP magnetic beads (Beckman Coulter) on Microlab Nimbus robotic platform (Hamilton) and 100 ng of cDNA was used for subsequent 10 rounds of amplification to generate aminoallyl-coupled cDNA for the hybridizations74,75. .. 17ul (~5 μg) of each Cy-5labelled (GE Healthcare) cDNA of patient’s sample and an equal amount of Cy-3labelled (GE Healthcare) cDNA of the reference pool were then hybridized together on our customized microarray chip using commercially available hybridization platform (Agilent) for 20 h at 70 °C with rotation at 10 rpm. .. Microarrays were washed and immediately scanned using Power Scanner (Tecan) at 10 μM resolution and with automated photomultiplier tubes gain adjustments to balance the signal intensities between both channels.

    Article Title: Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model.
    Article Snippet: .. 17 μl (~5 μg) of each Cy5-labeled (GE Healthcare) cDNA of the sample and an equal amount of Cy3labeled (GE Healthcare) cDNA of the reference pool were then hybridized together on a customized microarray chip using a commercially available hybridization platform (Agilent) for 20 h at 70°C with gentle rotation. .. Microarrays were washed and immediately scanned using Power Scanner (Tecan) at 10-μm resolution and with automated photomultiplier tubes gain adjustments to balance the signal intensities between both channels.

    Article Title: Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model
    Article Snippet: .. 17 μl (∼5 μg) of each Cy5-labeled (GE Healthcare) cDNA of the sample and an equal amount of Cy3-labeled (GE Healthcare) cDNA of the reference pool were then hybridized together on a customized microarray chip using a commercially available hybridization platform (Agilent) for 20 h at 70°C with gentle rotation. .. Microarrays were washed and immediately scanned using Power Scanner (Tecan) at 10-μm resolution and with automated photomultiplier tubes gain adjustments to balance the signal intensities between both channels.

    other:

    Article Title: Comprehensive analysis of the differential cellular and EBV miRNA expression profiles in mature T and NK cell lymphomas.
    Article Snippet: Epstein-Barr virus (EBV) has been detected in several subtypes of Mature T and NK cell lymphomas.. However, the impact of EBV status on the survival and the mechanism is still unknown.. Numerous recent investigations have demonstrated that the EBV-encoded microRNAs (EBV-miRNAs) were associated with the development and prognosis of lymphoma.

    Labeling:

    Article Title: Extracellular vesicles from bone marrow mesenchymal stem cells alleviate osteoporosis in mice through USP7-mediated YAP1 protein stability and the Wnt/β-catenin pathway.
    Article Snippet: Mesenchymal stem cells (MSCs) and their derived extracellular vesicles (EVs) have emerged as promising tools for promoting bone regeneration.. This study investigates the functions of EVs derived from bone marrow-derived MSCs (BMSCs) in osteoporosis (OP) and the molecular mechanism.. EVs were isolated from primary BMSCs in mice.

    Gentle:

    Article Title: Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model.
    Article Snippet: .. 17 μl (~5 μg) of each Cy5-labeled (GE Healthcare) cDNA of the sample and an equal amount of Cy3labeled (GE Healthcare) cDNA of the reference pool were then hybridized together on a customized microarray chip using a commercially available hybridization platform (Agilent) for 20 h at 70°C with gentle rotation. .. Microarrays were washed and immediately scanned using Power Scanner (Tecan) at 10-μm resolution and with automated photomultiplier tubes gain adjustments to balance the signal intensities between both channels.

    Article Title: Sterile protection against P. vivax malaria by repeated blood stage infection in the Aotus monkey model
    Article Snippet: .. 17 μl (∼5 μg) of each Cy5-labeled (GE Healthcare) cDNA of the sample and an equal amount of Cy3-labeled (GE Healthcare) cDNA of the reference pool were then hybridized together on a customized microarray chip using a commercially available hybridization platform (Agilent) for 20 h at 70°C with gentle rotation. .. Microarrays were washed and immediately scanned using Power Scanner (Tecan) at 10-μm resolution and with automated photomultiplier tubes gain adjustments to balance the signal intensities between both channels.



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