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immunohistochemical staining for th  (Novus Biologicals)


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    Structured Review

    Novus Biologicals immunohistochemical staining for th
    Immunohistochemical Staining For Th, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 268 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/th+immunostaining/Tyrosine+Hydroxylase+Antibody/pmc12370004-100-11-19
    Average 96 stars, based on 268 article reviews
    immunohistochemical staining for th - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Reduced Cortical Pyramidal Neuron Membrane Excitability and Synaptic Function in Parkinsonian Mice and Their Restoration by L-Dopa Treatment: Indirect Mediation by Striatal Dopaminergic Activity.
    Article Snippet: The free-floating sections were incubated with 1% normal donkey serum and 1% normal bovine serum (cat. # 017-000-121, 001-000-121, Jackson ImmunoResearch, West Groves, PA, USA), and 0.4% Triton X-100 in PBS for 1.5 h at room temperature to block non-specific binding and permeabilize the cell membrane, respectively (all primary and secondary antibodies were diluted in this same blocking and permeabilizing solution). .. Then, these free-floating sections were incubated for 24 h at room temperature with the primary antibody, a polyclonal tyrosine hydroxylase antibody produced in rabbit (diluted https://doi.org/10.3390/brainsci16030285 at 1:1000; Novus Biologicals, Littleton, CO, USA; cat. # NB300-109), and then rinsed thoroughly (10 min × 4) in PBS, followed by incubating with a donkey anti-rabbit secondary antibody conjugated with the green Alexa Fluor 488 (diluted at 1:400; Invitrogen, Waltham, MA, USA) for 3 h at room temperature followed by thorough rinsing in PBS (10 min × 4). .. For D1R staining, the primary D1R antibody is a rat monoclonal antibody purchased from Sigma (cat. # D-187, Sigma, St. Louis, MO, USA; used at 1:500 dilution).

    Article Title: Evaluation of Hydrogen Peroxide as an Environmental Model of Parkinson’s Disease in Drosophila Larvae
    Article Snippet: .. Preparations were incubated overnight at room temperature with agitation in primary antibodies diluted in blocking solution: rabbit anti-tyrosine hydroxylase (TH; 1:200; Novus Biologicals, NB300-109) and mouse anti-Bruchpilot (BRP; nc82; 1:100; Developmental Studies Hybridoma Bank [DSHB]). .. Following three 10-min washes in PBST, samples were incubated for 2 h at room temperature with agitation in goat anti-rabbit Cy3 (1:200; Fisher Scientific, AP132CMI) and goat anti-mouse Alexa Fluor 488 (1:200; Fisher Scientific, A11001) secondary antibodies diluted in blocking solution.

    Produced:

    Article Title: Reduced Cortical Pyramidal Neuron Membrane Excitability and Synaptic Function in Parkinsonian Mice and Their Restoration by L-Dopa Treatment: Indirect Mediation by Striatal Dopaminergic Activity.
    Article Snippet: The free-floating sections were incubated with 1% normal donkey serum and 1% normal bovine serum (cat. # 017-000-121, 001-000-121, Jackson ImmunoResearch, West Groves, PA, USA), and 0.4% Triton X-100 in PBS for 1.5 h at room temperature to block non-specific binding and permeabilize the cell membrane, respectively (all primary and secondary antibodies were diluted in this same blocking and permeabilizing solution). .. Then, these free-floating sections were incubated for 24 h at room temperature with the primary antibody, a polyclonal tyrosine hydroxylase antibody produced in rabbit (diluted https://doi.org/10.3390/brainsci16030285 at 1:1000; Novus Biologicals, Littleton, CO, USA; cat. # NB300-109), and then rinsed thoroughly (10 min × 4) in PBS, followed by incubating with a donkey anti-rabbit secondary antibody conjugated with the green Alexa Fluor 488 (diluted at 1:400; Invitrogen, Waltham, MA, USA) for 3 h at room temperature followed by thorough rinsing in PBS (10 min × 4). .. For D1R staining, the primary D1R antibody is a rat monoclonal antibody purchased from Sigma (cat. # D-187, Sigma, St. Louis, MO, USA; used at 1:500 dilution).

    other:

    Article Title: Hair metal dysregulation in Parkinson’s disease: Implications for diagnosis and gut-brain axis involvement
    Article Snippet: Anti-TH , Novus , Cat# NB300-109; RRID: AB_10077691.

    Blocking Assay:

    Article Title: Evaluation of Hydrogen Peroxide as an Environmental Model of Parkinson’s Disease in Drosophila Larvae
    Article Snippet: .. Preparations were incubated overnight at room temperature with agitation in primary antibodies diluted in blocking solution: rabbit anti-tyrosine hydroxylase (TH; 1:200; Novus Biologicals, NB300-109) and mouse anti-Bruchpilot (BRP; nc82; 1:100; Developmental Studies Hybridoma Bank [DSHB]). .. Following three 10-min washes in PBST, samples were incubated for 2 h at room temperature with agitation in goat anti-rabbit Cy3 (1:200; Fisher Scientific, AP132CMI) and goat anti-mouse Alexa Fluor 488 (1:200; Fisher Scientific, A11001) secondary antibodies diluted in blocking solution.



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    Novus Biologicals immunohistochemical staining against tyrosine hydroxylase th
    Representative image of nerve with HE and TH. A representative image of nerve with HE (A) and a corresponding image stained by TH (B) in the control group. A representative image of nerve with HE (C) and a corresponding image stained by TH (D) in the SBRT group. (E) <t>Immunohistochemical</t> staining showed mean intensity of TH of all sympathetic nerves between the control group and SBRT group. HE, hematoxylin and eosin; TH, tyrosine <t>hydroxylase;</t> Con, control group; SBRT, stereotactic body radiotherapy group. Non-normally distributed quantitative variables were compared using the Mann–Whitney U test. Comparisons in same group before and after establishing ATEPAH rabbits: p > 0.05. n = 6 rabbits per group for all analyses.
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    Representative image of nerve with HE and TH. A representative image of nerve with HE (A) and a corresponding image stained by TH (B) in the control group. A representative image of nerve with HE (C) and a corresponding image stained by TH (D) in the SBRT group. (E) <t>Immunohistochemical</t> staining showed mean intensity of TH of all sympathetic nerves between the control group and SBRT group. HE, hematoxylin and eosin; TH, tyrosine <t>hydroxylase;</t> Con, control group; SBRT, stereotactic body radiotherapy group. Non-normally distributed quantitative variables were compared using the Mann–Whitney U test. Comparisons in same group before and after establishing ATEPAH rabbits: p > 0.05. n = 6 rabbits per group for all analyses.
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    Representative image of nerve with HE and TH. A representative image of nerve with HE (A) and a corresponding image stained by TH (B) in the control group. A representative image of nerve with HE (C) and a corresponding image stained by TH (D) in the SBRT group. (E) <t>Immunohistochemical</t> staining showed mean intensity of TH of all sympathetic nerves between the control group and SBRT group. HE, hematoxylin and eosin; TH, tyrosine <t>hydroxylase;</t> Con, control group; SBRT, stereotactic body radiotherapy group. Non-normally distributed quantitative variables were compared using the Mann–Whitney U test. Comparisons in same group before and after establishing ATEPAH rabbits: p > 0.05. n = 6 rabbits per group for all analyses.
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    Image Search Results


    Representative image of nerve with HE and TH. A representative image of nerve with HE (A) and a corresponding image stained by TH (B) in the control group. A representative image of nerve with HE (C) and a corresponding image stained by TH (D) in the SBRT group. (E) Immunohistochemical staining showed mean intensity of TH of all sympathetic nerves between the control group and SBRT group. HE, hematoxylin and eosin; TH, tyrosine hydroxylase; Con, control group; SBRT, stereotactic body radiotherapy group. Non-normally distributed quantitative variables were compared using the Mann–Whitney U test. Comparisons in same group before and after establishing ATEPAH rabbits: p > 0.05. n = 6 rabbits per group for all analyses.

    Journal: Frontiers in Medicine

    Article Title: Stereotactic body radiotherapy: a new non-invasive way to conduct pulmonary artery denervation

    doi: 10.3389/fmed.2025.1607638

    Figure Lengend Snippet: Representative image of nerve with HE and TH. A representative image of nerve with HE (A) and a corresponding image stained by TH (B) in the control group. A representative image of nerve with HE (C) and a corresponding image stained by TH (D) in the SBRT group. (E) Immunohistochemical staining showed mean intensity of TH of all sympathetic nerves between the control group and SBRT group. HE, hematoxylin and eosin; TH, tyrosine hydroxylase; Con, control group; SBRT, stereotactic body radiotherapy group. Non-normally distributed quantitative variables were compared using the Mann–Whitney U test. Comparisons in same group before and after establishing ATEPAH rabbits: p > 0.05. n = 6 rabbits per group for all analyses.

    Article Snippet: After the histological evaluation of all slides, sections from pulmonary arteries in each group were chosen for subsequent immunohistochemical staining against tyrosine hydroxylase (TH) (dilution 1:100, NB300-110SS, Novus).

    Techniques: Staining, Control, Immunohistochemical staining, MANN-WHITNEY