tetramethylbenzidine substrate solution (Rockland Immunochemicals)

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Rockland Immunochemicals tetramethylbenzidine substrate solution
Tetramethylbenzidine Substrate Solution, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tetramethylbenzidine/TMB+Prestained+Red+ELISA+Peroxidase+Substrate/pm41677760-85-21-24
Average 94 stars, based on 5 article reviews

tetramethylbenzidine substrate solution - by Bioz Stars, 2026-09

94/100 stars

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Incubation:

Article Title: Systemic and mucosal immune signatures of protection against SARS-CoV-2 transmission in humans.
Article Snippet: .. After incubation with the corresponding secondary HRP-conjugated antibodies, plates were washed four times with PBS-T with shaking using the plate washer and 100 μL of TMBE (Rockland) was added to all wells for 10 min. ..

Article Title: Systemic and mucosal immune signatures of protection against SARS-CoV-2 transmission in humans
Article Snippet: .. After incubation with the corresponding secondary HRP-conjugated antibodies, plates were washed four times with PBS-T with shaking using the plate washer and 100 μL of TMBE (Rockland) was added to all wells for 10 min. ..

Article Title: Induction of Autoimmune Myocarditis in Diversity Outbred Mice
Article Snippet: Serum samples diluted 1:100 were then added in duplicates and incubated at 37 °C for 1 h. After washing, horseradish peroxide (HRP)-labeled goat anti-mouse total Ig, IgM, IgG1, IgG2a, IgG2b, and IgG3 (cat. no. 5300-05), as well as IgG2c (cat. no. 1079-05), diluted 1:500 in assay buffer, were added as secondary antibodies (Southern Biotech, Birmingham, AL, USA). .. The plates were incubated at room temperature for 2 h, followed by washing and the addition of 100 μL of 1× tetramethylbenzidine substrate solution (Rockland Immunochemicals, Pottstown, PA, USA; cat. no. TMBE-RED-1000). .. Reactions were stopped using 1 M phosphoric acid, and the optical density (OD) values were measured at 450 nm using an automated ELISA reader (BioTek Instruments, Winooski, VT, USA).

Article Title: Induction of Autoimmune Myocarditis in Diversity Outbred Mice.
Article Snippet: Serum samples diluted 1:100 were then added in duplicates and incubated at 37 ◦C for 1 h. After washing, horseradish peroxide (HRP)-labeled goat anti-mouse total Ig, IgM, IgG1, IgG2a, IgG2b, and IgG3 (cat. no. 5300-05), as well as IgG2c (cat. no. 1079-05), diluted 1:500 in assay buffer, were added as secondary antibodies (Southern Biotech, Birmingham, AL, USA). .. The plates were incubated at room temperature for 2 h, followed by washing and the addition of 100 μL of 1× tetramethylbenzidine substrate solution (Rockland Immunochemicals, Pottstown, PA, USA; cat. no. TMBE-RED-1000). .. Reactions were stopped using 1 M phosphoric acid, and the optical density (OD) values were measured at 450 nm using an automated ELISA reader (BioTek Instruments, Winooski, VT, USA).

Infection:

Article Title: Systemic and mucosal immune signatures of protection against SARS-CoV-2 transmission in humans.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human IgG-HRP antibody (Fc-specific) Sigma-Aldrich Cat#A0170; RRID: AB_257868 Anti-human IgM-HRP antibody Sigma-Aldrich Cat#A6907; RRID: AB_258318 Anti-human IgA-HRP antibody (α-chain) Sigma-Aldrich Cat#A0295; RRID: AB-257876 Anti-human sIgA antibody Millipore Cat#411423; RRID: AB_10681347 Anti-mouse IgG-HRP antibody Abcam Cat#ab6823; RRID: AB_955395 Biological samples Human serum samples from COVID-19 infected and exposed individuals from ‘The CIDS Cohort’ University Hospital of Burgos, Burgos (Spain) This paper Human nasopharyngeal swabs samples from COVID-19 infected and exposed individuals from ‘The CIDS Cohort’ University Hospital of Burgos, Burgos (Spain) This paper Chemicals, peptides, and recombinant proteins SARS-CoV-2 full-length spike protein Sino Biological Cat#40589-V08H4 SARS-CoV-2 S1 protein Sino Biological Cat#40591-V08H SARS-CoV-2 S2 protein Sino Biological Cat#40590-V08H1 SARS-CoV-2 RBD protein Sino Biological Cat#40592-V08H80 OC43 full-length spike protein Sino Biological Cat#40607-V08B OC43 S1 protein Sino Biological Cat#40607-V08H1 OC43 S2 protein Sino Biological Cat#40607-V08B1 229e full-length spike protein Sino Biological Cat#40605-V08B 3,3’,5,5’ -Tetra- methylbenzidine (TMBE) Rockland CatTMBE-1000 Sulfuric Acid 2M Fisher Scientific Cat#S25898 Critical commercial assays E.Z.N.A Viral RNA kit Omega Bio-Tek Cat#R6874 QuantiFast Pathogen RT-PCR + IC Kit QIAGEN Cat# 211454 Oligonucleotides RT-qPCR primers RUO Kit Integrated DNA Technologies Cat#10006713 Software and algorithms IBM SPSS Statistics N/A GraphPad Prism 9 GraphPad N/A Other Phosphate-buffered saline (PBS) Corning Cat#21-040-CV Tween 20 Fisher Scientific Cat#BP337-100 Non-fat milk powder RPI Cat#M17200-500 Triton X-100 Fisher Scientific Cat#BP151-500 4 HBX 96-well microtiter plates Thermo Fisher Scientific Cat#3855 LightCycler 480 Multiwell Plate 384 Roche Cat#04729749001 LightCycler 480 II Roche N/A 405 TS microplate washer BioTek N/A Synergy 4 plate reader BioTek N/A Cell Reports Medicine 6, 102505, December 16, 2025 e1 ..

Recombinant:

Reverse Transcription Polymerase Chain Reaction:

Quantitative RT-PCR:

Software:

Saline:

other:

Article Title: Systemic and mucosal immune signatures of protection against SARS-CoV-2 transmission in humans
Article Snippet: 3,3’,5,5’ -Tetra- methylbenzidine (TMBE) , Rockland , CatTMBE-1000.

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