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tetramethylbenzidine chromogenic solution (tmb) it0250  (Beijing Solarbio Science)


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    Beijing Solarbio Science tetramethylbenzidine chromogenic solution (tmb) it0250
    Tetramethylbenzidine Chromogenic Solution (Tmb) It0250, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tetramethylbenzidine+tmb+solution/tetramethylbenzidine+chromogenic+solution++tmb++it0250/pmc10298237-167-120-125
    Average 90 stars, based on 1 article reviews
    tetramethylbenzidine chromogenic solution (tmb) it0250 - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Virus:

    Article Title: The Role of H2-Calponin Antigen in Cancer Metastasis: Presence of Autoantibodies in Liver Cancer Patients
    Article Snippet: After washing, the plates were blocked with 0.5% bovine serum albumin (BSA) (A8010, Solabor Bioengineering Co., Ltd., Beijing, China) at 37 °C for 1 h. The serum of HCC, hepatitis B virus, liver cirrhosis, and normal tissue was diluted 1024 times with PBST, then 100 μL/well was added to the ELISA plate, and it was incubated at 37 °C for 1 h. Biotin-goat anti-human IgG (H + L) antibody (K1218, APExBIO, Shanghai, Beijing) was diluted 20,000 times, 100 μL/well was added to the ELISA plate, and it was incubated at 37 °C for 1 h. Streptavidin-HRP was diluted 9000 times, then 100 μL/well was added to the ELISA plate, and it was incubated at 37 °C for 1 h. Then, tetramethylbenzidine chromogenic solution (TMB) (IT0250, Solarbio Bioengineering Co., Ltd., Beijing, China) was added to the ELISA plate, with 100 μL/well, and it was incubated away from light for 15 min.

    Article Title: The Role of H2-Calponin Antigen in Cancer Metastasis: Presence of Autoantibodies in Liver Cancer Patients.
    Article Snippet: After washing, the plates were blocked with 0.5% bovine serum albumin (BSA) (A8010, Solabor Bioengineering Co., Ltd., Beijing, China) at 37 ◦C for 1 h. The serum of HCC, hepatitis B virus, liver cirrhosis, and normal tissue was diluted 1024 times with PBST, then 100 μL/well was added to the ELISA plate, and it was incubated at 37 ◦C for 1 h. Biotin-goat anti-human IgG (H + L) antibody (K1218, APExBIO, Shanghai, Beijing) was diluted 20,000 times, 100 μL/well was added to the ELISA plate, and it was incubated at 37 ◦C for 1 h. Streptavidin-HRP was diluted 9000 times, then 100 μL/well was added to the ELISA plate, and it was incubated at 37 ◦C for 1 h. Then, tetramethylbenzidine chromogenic solution (TMB) (IT0250, Solarbio Bioengineering Co., Ltd., Beijing, China) was added to the ELISA plate, with 100 μL/well, and it was incubated away from light for 15 min.

    Enzyme-linked Immunosorbent Assay:

    Article Title: The Role of H2-Calponin Antigen in Cancer Metastasis: Presence of Autoantibodies in Liver Cancer Patients
    Article Snippet: After washing, the plates were blocked with 0.5% bovine serum albumin (BSA) (A8010, Solabor Bioengineering Co., Ltd., Beijing, China) at 37 °C for 1 h. The serum of HCC, hepatitis B virus, liver cirrhosis, and normal tissue was diluted 1024 times with PBST, then 100 μL/well was added to the ELISA plate, and it was incubated at 37 °C for 1 h. Biotin-goat anti-human IgG (H + L) antibody (K1218, APExBIO, Shanghai, Beijing) was diluted 20,000 times, 100 μL/well was added to the ELISA plate, and it was incubated at 37 °C for 1 h. Streptavidin-HRP was diluted 9000 times, then 100 μL/well was added to the ELISA plate, and it was incubated at 37 °C for 1 h. Then, tetramethylbenzidine chromogenic solution (TMB) (IT0250, Solarbio Bioengineering Co., Ltd., Beijing, China) was added to the ELISA plate, with 100 μL/well, and it was incubated away from light for 15 min.

    Article Title: The Role of H2-Calponin Antigen in Cancer Metastasis: Presence of Autoantibodies in Liver Cancer Patients.
    Article Snippet: After washing, the plates were blocked with 0.5% bovine serum albumin (BSA) (A8010, Solabor Bioengineering Co., Ltd., Beijing, China) at 37 ◦C for 1 h. The serum of HCC, hepatitis B virus, liver cirrhosis, and normal tissue was diluted 1024 times with PBST, then 100 μL/well was added to the ELISA plate, and it was incubated at 37 ◦C for 1 h. Biotin-goat anti-human IgG (H + L) antibody (K1218, APExBIO, Shanghai, Beijing) was diluted 20,000 times, 100 μL/well was added to the ELISA plate, and it was incubated at 37 ◦C for 1 h. Streptavidin-HRP was diluted 9000 times, then 100 μL/well was added to the ELISA plate, and it was incubated at 37 ◦C for 1 h. Then, tetramethylbenzidine chromogenic solution (TMB) (IT0250, Solarbio Bioengineering Co., Ltd., Beijing, China) was added to the ELISA plate, with 100 μL/well, and it was incubated away from light for 15 min.

    Incubation:

    Article Title: The Role of H2-Calponin Antigen in Cancer Metastasis: Presence of Autoantibodies in Liver Cancer Patients
    Article Snippet: After washing, the plates were blocked with 0.5% bovine serum albumin (BSA) (A8010, Solabor Bioengineering Co., Ltd., Beijing, China) at 37 °C for 1 h. The serum of HCC, hepatitis B virus, liver cirrhosis, and normal tissue was diluted 1024 times with PBST, then 100 μL/well was added to the ELISA plate, and it was incubated at 37 °C for 1 h. Biotin-goat anti-human IgG (H + L) antibody (K1218, APExBIO, Shanghai, Beijing) was diluted 20,000 times, 100 μL/well was added to the ELISA plate, and it was incubated at 37 °C for 1 h. Streptavidin-HRP was diluted 9000 times, then 100 μL/well was added to the ELISA plate, and it was incubated at 37 °C for 1 h. Then, tetramethylbenzidine chromogenic solution (TMB) (IT0250, Solarbio Bioengineering Co., Ltd., Beijing, China) was added to the ELISA plate, with 100 μL/well, and it was incubated away from light for 15 min.

    Article Title: The Role of H2-Calponin Antigen in Cancer Metastasis: Presence of Autoantibodies in Liver Cancer Patients.
    Article Snippet: After washing, the plates were blocked with 0.5% bovine serum albumin (BSA) (A8010, Solabor Bioengineering Co., Ltd., Beijing, China) at 37 ◦C for 1 h. The serum of HCC, hepatitis B virus, liver cirrhosis, and normal tissue was diluted 1024 times with PBST, then 100 μL/well was added to the ELISA plate, and it was incubated at 37 ◦C for 1 h. Biotin-goat anti-human IgG (H + L) antibody (K1218, APExBIO, Shanghai, Beijing) was diluted 20,000 times, 100 μL/well was added to the ELISA plate, and it was incubated at 37 ◦C for 1 h. Streptavidin-HRP was diluted 9000 times, then 100 μL/well was added to the ELISA plate, and it was incubated at 37 ◦C for 1 h. Then, tetramethylbenzidine chromogenic solution (TMB) (IT0250, Solarbio Bioengineering Co., Ltd., Beijing, China) was added to the ELISA plate, with 100 μL/well, and it was incubated away from light for 15 min.



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