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tb green test kit  (Bio-Rad)


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    Structured Review

    Bio-Rad tb green test kit
    Tb Green Test Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/test+kit/iTaq+Universal+SYBR+Green+One-Step+Kit/pmc12993172-116-23-31
    Average 96 stars, based on 1313 article reviews
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    Related Articles

    Targeted Gene Expression:

    Article Title: HIV–opioid interactions in the brain microenvironment drive neuroimmune, synaptic, and chromatin-associated transcriptomic remodeling during neurological impairment: insights from human and murine models
    Article Snippet: Cycle threshold (Ct) values were analyzed using the manufacturer’s GeneGlobe Data Analysis Center (https://geneglobe.qiagen.com/us/analyze). .. For targeted gene expression analysis, mouse brain RNA was subjected to quantitative PCR using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad; Hercules, CA, USA) on a CFX96 Real-Time PCR System (Bio-Rad). ..

    Analysis:

    Article Title: HIV–opioid interactions in the brain microenvironment drive neuroimmune, synaptic, and chromatin-associated transcriptomic remodeling during neurological impairment: insights from human and murine models
    Article Snippet: Cycle threshold (Ct) values were analyzed using the manufacturer’s GeneGlobe Data Analysis Center (https://geneglobe.qiagen.com/us/analyze). .. For targeted gene expression analysis, mouse brain RNA was subjected to quantitative PCR using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad; Hercules, CA, USA) on a CFX96 Real-Time PCR System (Bio-Rad). ..

    Real-time Polymerase Chain Reaction:

    Article Title: HIV–opioid interactions in the brain microenvironment drive neuroimmune, synaptic, and chromatin-associated transcriptomic remodeling during neurological impairment: insights from human and murine models
    Article Snippet: Cycle threshold (Ct) values were analyzed using the manufacturer’s GeneGlobe Data Analysis Center (https://geneglobe.qiagen.com/us/analyze). .. For targeted gene expression analysis, mouse brain RNA was subjected to quantitative PCR using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad; Hercules, CA, USA) on a CFX96 Real-Time PCR System (Bio-Rad). ..

    Article Title: Scalable synthetic peptide hydrogel enables self-organized luminal cavity architecture within hiPSC 3D colonies supporting functional hepatocyte differentiation
    Article Snippet: Upon completion of the differentiation process, the 3D colonies were retrieved, and total RNA was extracted from the samples with a Direct-zol RNA MiniPrep Kit (Zymo Research Corp., Irvine, CA, USA) and diluted to 20 ng/μL. .. RT‒qPCR was conducted using the Bio-Rad CFX96TM TouchTM Real-time PCR Detection System and an iTaq Universal SYBR Green One-Step Kit (Bio-Rad, Hercules, CA, USA). ..

    Article Title: Enhancing reverse methanogenesis using antisense RNA-mediated silencing
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Comparison of Human Costal-Derived Chondrocyte Donor Sites and Microtia-Derived Chondrocytes for Auricular Tissue Engineering.
    Article Snippet: cDNA was prepared from 1 μg of RNA via the ProScript II First Strand cDNA Synthesis Kit (New England Biolabs, USA) according to the manufacturer’s instructions. .. Real-time quantitative PCR was performed using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad, USA) on the CFX Duet Real-Time PCR System (Bio-Rad, USA). ..

    SYBR Green Assay:

    Article Title: HIV–opioid interactions in the brain microenvironment drive neuroimmune, synaptic, and chromatin-associated transcriptomic remodeling during neurological impairment: insights from human and murine models
    Article Snippet: Cycle threshold (Ct) values were analyzed using the manufacturer’s GeneGlobe Data Analysis Center (https://geneglobe.qiagen.com/us/analyze). .. For targeted gene expression analysis, mouse brain RNA was subjected to quantitative PCR using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad; Hercules, CA, USA) on a CFX96 Real-Time PCR System (Bio-Rad). ..

    Article Title: Biomimetic de novo construction of hierarchically aligned and gradient-mineralized collagen for tendon-bone integrated regeneration
    Article Snippet: After 14 days of culture, cells were digested with 0.25% trypsin solution, and mRNA was extracted by TRIzol reagent (Bio-Rad, USA) according to the manufacturer's instructions. .. The extracted mRNA was reverse transcribed into cDNA by test kit (iScript1TM cDNA Bio-Rad kit USA), and RT-PCR was performed by using a TB Green test kit (iTaq Universal SYBR Green, Bio-Rad, USA) as per the manufacturer's protocol (n = 3). ..

    Article Title: Scalable synthetic peptide hydrogel enables self-organized luminal cavity architecture within hiPSC 3D colonies supporting functional hepatocyte differentiation
    Article Snippet: Upon completion of the differentiation process, the 3D colonies were retrieved, and total RNA was extracted from the samples with a Direct-zol RNA MiniPrep Kit (Zymo Research Corp., Irvine, CA, USA) and diluted to 20 ng/μL. .. RT‒qPCR was conducted using the Bio-Rad CFX96TM TouchTM Real-time PCR Detection System and an iTaq Universal SYBR Green One-Step Kit (Bio-Rad, Hercules, CA, USA). ..

    Article Title: Smooth muscle cell mineralocorticoid receptor does not contribute to atherosclerosis in male or female ApoE-knockout mice
    Article Snippet: RNA was eluted in RNase-free water and quantified using a NanoDrop spectrophotometer. .. One-step RT-qPCR was performed using iTaq Universal SYBR Green One-Step Kit (Bio-Rad). ..

    Article Title: Enhancing reverse methanogenesis using antisense RNA-mediated silencing
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Abscisic acid and epibrassinolide reprogram barley drought responses across multiple regulatory layers
    Article Snippet: The concentration and purity of the isolates were determined using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific). .. cDNA was synthesized from total RNA (100 ng/μl) using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad, Hercules, USA; www.bio-rad.com) according to the manufacturer’s protocol. ..

    Article Title: Comparison of Human Costal-Derived Chondrocyte Donor Sites and Microtia-Derived Chondrocytes for Auricular Tissue Engineering.
    Article Snippet: cDNA was prepared from 1 μg of RNA via the ProScript II First Strand cDNA Synthesis Kit (New England Biolabs, USA) according to the manufacturer’s instructions. .. Real-time quantitative PCR was performed using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad, USA) on the CFX Duet Real-Time PCR System (Bio-Rad, USA). ..

    Reverse Transcription:

    Article Title: Biomimetic de novo construction of hierarchically aligned and gradient-mineralized collagen for tendon-bone integrated regeneration
    Article Snippet: After 14 days of culture, cells were digested with 0.25% trypsin solution, and mRNA was extracted by TRIzol reagent (Bio-Rad, USA) according to the manufacturer's instructions. .. The extracted mRNA was reverse transcribed into cDNA by test kit (iScript1TM cDNA Bio-Rad kit USA), and RT-PCR was performed by using a TB Green test kit (iTaq Universal SYBR Green, Bio-Rad, USA) as per the manufacturer's protocol (n = 3). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Biomimetic de novo construction of hierarchically aligned and gradient-mineralized collagen for tendon-bone integrated regeneration
    Article Snippet: After 14 days of culture, cells were digested with 0.25% trypsin solution, and mRNA was extracted by TRIzol reagent (Bio-Rad, USA) according to the manufacturer's instructions. .. The extracted mRNA was reverse transcribed into cDNA by test kit (iScript1TM cDNA Bio-Rad kit USA), and RT-PCR was performed by using a TB Green test kit (iTaq Universal SYBR Green, Bio-Rad, USA) as per the manufacturer's protocol (n = 3). ..

    Quantitative RT-PCR:

    Article Title: Smooth muscle cell mineralocorticoid receptor does not contribute to atherosclerosis in male or female ApoE-knockout mice
    Article Snippet: RNA was eluted in RNase-free water and quantified using a NanoDrop spectrophotometer. .. One-step RT-qPCR was performed using iTaq Universal SYBR Green One-Step Kit (Bio-Rad). ..

    Synthesized:

    Article Title: Abscisic acid and epibrassinolide reprogram barley drought responses across multiple regulatory layers
    Article Snippet: The concentration and purity of the isolates were determined using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific). .. cDNA was synthesized from total RNA (100 ng/μl) using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad, Hercules, USA; www.bio-rad.com) according to the manufacturer’s protocol. ..



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    Image Search Results


    Genetic and Pharmacological Inhibition of Nupr1 Attenuates Ang II Induced Mitochondrial Apoptosis in VSMCs. (A) Cell ability was evaluated by CCK8 assay ( n = 8 per group). (B) Lactate production of indicated groups ( n = 6 per group). (C) Representative images of TEM in VSMCs from the indicated groups. (D) Quantification of mitochondrial size in VSMCs from indicated groups ( n = 6 per group). (E) Quantification of circularity index in VSMC from indicated groups ( n = 6 per group). (F) Real‐time monitoring of oxygen consumption rate (OCR) in indicated groups detected by O2k. (G–I) Quantification of ATP‐coupled respiration, mitochondrial basal respiration, and maximal respiration from indicated groups ( n = 6 per group). (J) Representative images of calcein and Annexin V fluorescence results showing mPTP opening and apoptosis in indicated groups. Stronger green fluorescence represents a lower degree of mPTP opening, while diminished fluorescence indicates increased mPTP opening. Stronger red fluorescence represents a higher apoptosis rate. (K, L) Quantification of fluorescence intensity, including Annexin V and Calcein ( n = 6 per group). (M) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (N) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group). (O) Quantification of maximal respiration from indicated groups ( n = 6 per group). (P) Real‐time monitoring of OCR in indicated groups detected by O2k. (Q, R) Quantification of ATP‐coupled respiration and mitochondrial basal respiration from indicated groups ( n = 6 per group). (S) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (T) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group).

    Journal: Advanced Science

    Article Title: Targeting Nupr1‐Mediated p53 Mitochondrial Sequestration Alleviates Aortic Dissection by Restoring the Gadd45b‐Dependent Survival Program

    doi: 10.1002/advs.78143

    Figure Lengend Snippet: Genetic and Pharmacological Inhibition of Nupr1 Attenuates Ang II Induced Mitochondrial Apoptosis in VSMCs. (A) Cell ability was evaluated by CCK8 assay ( n = 8 per group). (B) Lactate production of indicated groups ( n = 6 per group). (C) Representative images of TEM in VSMCs from the indicated groups. (D) Quantification of mitochondrial size in VSMCs from indicated groups ( n = 6 per group). (E) Quantification of circularity index in VSMC from indicated groups ( n = 6 per group). (F) Real‐time monitoring of oxygen consumption rate (OCR) in indicated groups detected by O2k. (G–I) Quantification of ATP‐coupled respiration, mitochondrial basal respiration, and maximal respiration from indicated groups ( n = 6 per group). (J) Representative images of calcein and Annexin V fluorescence results showing mPTP opening and apoptosis in indicated groups. Stronger green fluorescence represents a lower degree of mPTP opening, while diminished fluorescence indicates increased mPTP opening. Stronger red fluorescence represents a higher apoptosis rate. (K, L) Quantification of fluorescence intensity, including Annexin V and Calcein ( n = 6 per group). (M) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (N) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group). (O) Quantification of maximal respiration from indicated groups ( n = 6 per group). (P) Real‐time monitoring of OCR in indicated groups detected by O2k. (Q, R) Quantification of ATP‐coupled respiration and mitochondrial basal respiration from indicated groups ( n = 6 per group). (S) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (T) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group).

    Article Snippet: According to mPTP test kit instructions (C2009S, Beyotime), 200 μL of Calcein AM staining solution and fluorescence quenching working solution were added to each well after the respective cell treatments.

    Techniques: Inhibition, CCK-8 Assay, Real-time Monitoring, Fluorescence, Flow Cytometry, Analysis, Staining

    Gadd45b is Indispensable for the Protective Effects of Nupr1 Deficiency Against Mitochondrial Apoptosis. (A) Enrichment of KEGG pathways. (B) GSEA analysis in VSMCs treated with Ang II stimulation and Nupr1 deficiency. (C) Chordal diagram of GO pathways related with cell fate and the contributing DEPs. (D) Stem plot of DEPs in the apoptosis pathway from the indicated groups. (E) RT‐qPCR analysis of mRNA levels of candidate genes in VSMCs. Data were presented as relative fold change to controls ( n = 6 per group). (F) Expressions of Gadd45b in VSMCs measured by Western blot and its quantification from indicated groups measured by Western blot ( n = 5 per group). (G, H) Representative images of ROS staining in indicated groups. Semi‐quantitative analysis of mean fluorescence intensity in indicated groups ( n = 6 per group). (I) Representative images of calcein and Annexin V fluorescence results showing mPTP opening in indicated groups. (J) Quantification of fluorescence intensity, including Annexin V and mPTP ( n = 3 per group). (K) Co‐immunoprecipitation and Western blot assays of Myc‐Nupr1 and Gadd45b. (L) Co‐immunoprecipitation assays of Myc‐Gadd45b and Nupr1.

    Journal: Advanced Science

    Article Title: Targeting Nupr1‐Mediated p53 Mitochondrial Sequestration Alleviates Aortic Dissection by Restoring the Gadd45b‐Dependent Survival Program

    doi: 10.1002/advs.78143

    Figure Lengend Snippet: Gadd45b is Indispensable for the Protective Effects of Nupr1 Deficiency Against Mitochondrial Apoptosis. (A) Enrichment of KEGG pathways. (B) GSEA analysis in VSMCs treated with Ang II stimulation and Nupr1 deficiency. (C) Chordal diagram of GO pathways related with cell fate and the contributing DEPs. (D) Stem plot of DEPs in the apoptosis pathway from the indicated groups. (E) RT‐qPCR analysis of mRNA levels of candidate genes in VSMCs. Data were presented as relative fold change to controls ( n = 6 per group). (F) Expressions of Gadd45b in VSMCs measured by Western blot and its quantification from indicated groups measured by Western blot ( n = 5 per group). (G, H) Representative images of ROS staining in indicated groups. Semi‐quantitative analysis of mean fluorescence intensity in indicated groups ( n = 6 per group). (I) Representative images of calcein and Annexin V fluorescence results showing mPTP opening in indicated groups. (J) Quantification of fluorescence intensity, including Annexin V and mPTP ( n = 3 per group). (K) Co‐immunoprecipitation and Western blot assays of Myc‐Nupr1 and Gadd45b. (L) Co‐immunoprecipitation assays of Myc‐Gadd45b and Nupr1.

    Article Snippet: According to mPTP test kit instructions (C2009S, Beyotime), 200 μL of Calcein AM staining solution and fluorescence quenching working solution were added to each well after the respective cell treatments.

    Techniques: Analysis, Quantitative RT-PCR, Western Blot, Staining, Fluorescence, Co-Immunoprecipitation Assay, Significance Assay