test compound hil-15, lot no: 6870-011  (Immunex Corporation)

Journal: Microbiology Spectrum

Article Title: Exploring the anti-biofilm effect of darobactin B and colistin in static and dynamic environments

doi: 10.1128/spectrum.02868-25

Figure Lengend Snippet: Results of microplate-based static assay. P. aeruginosa ATCC 27853 biofilms after 6-h treatment with colistin ( A ) and darobactin B ( B ): bar graphs showing the mean ± standard deviation of calculated area of red-stained, green-stained, and total cells of the biofilms after the treatment (i); representative fluorescence images (ii) (images of full assay plates are available in ); and CFU count results (iii). Statistical analysis shows significant differences between the treatments with the five-highest antibiotic concentrations compared to untreated biofilms (**** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05; ns, not significant).

Article Snippet: First, we used a microplate-based biofilm eradication assay to determine the primary activity of the test compounds against P. aeruginosa ATCC 27853.

Techniques: Standard Deviation, Staining, Fluorescence

Journal: Microbiology Spectrum

Article Title: Exploring the anti-biofilm effect of darobactin B and colistin in static and dynamic environments

doi: 10.1128/spectrum.02868-25

Figure Lengend Snippet: Analysis of P. aeruginosa biofilm formation. ( A ) Surface coverage of P. aeruginosa ATCC 27853 during the biofilm formation step. ( B ) Representative bright-field microscopic images of various developmental stages of P. aeruginosa ATCC 27853 biofilms: initial attachment (0 h), strong adhesion (8 h), early developmental stage (microcolony formation—12 h), maturation (macrocolony formation—16, 24, and 48 h).

Article Snippet: First, we used a microplate-based biofilm eradication assay to determine the primary activity of the test compounds against P. aeruginosa ATCC 27853.

Techniques:

Journal: Microbiology Spectrum

Article Title: Exploring the anti-biofilm effect of darobactin B and colistin in static and dynamic environments

doi: 10.1128/spectrum.02868-25

Figure Lengend Snippet: ( A ) Biofilms of P. aeruginosa ATCC 27853 stained with SYBR Green I and PI before and after antibiotic treatment, as well as during the regrowth phase. Bottom: effect of PEN, CST, and DAR B on mature P. aeruginosa ATCC 27853 biofilms during the 3-h treatment ( B ) and during the regrowth phase ( C ). The ratio of membrane-compromised (red-stained) cells to total cells was calculated over time during the treatment and regrowth phases.

Article Snippet: First, we used a microplate-based biofilm eradication assay to determine the primary activity of the test compounds against P. aeruginosa ATCC 27853.

Techniques: Staining, SYBR Green Assay, Membrane

Journal: Microbiology Spectrum

Article Title: Exploring the anti-biofilm effect of darobactin B and colistin in static and dynamic environments

doi: 10.1128/spectrum.02868-25

Figure Lengend Snippet: ( A ) Representative microscopic images of P. aeruginosa ATCC 27853 biofilms stained with SYBR Green I and PI before and after treatment(s) with 64 µg/mL DAR B, as well as during the 48-h regrowth phase in the multidose experiments. Each treatment involved the application of 64 µg/mL DARB for 2 h with a 24-h interval. Bottom: effect of DAR B on mature P. aeruginosa ATCC 27853 biofilms during the sequential 2-h treatments ( B ) and the regrowth phase ( C ). The ratio of membrane-compromised (red-stained) cells to total cells and percentage of surface coverage were calculated over time during the treatment and regrowth phases (simplified; detailed calculations of each 2-h treatment are shown in ).

Article Snippet: First, we used a microplate-based biofilm eradication assay to determine the primary activity of the test compounds against P. aeruginosa ATCC 27853.

Techniques: Staining, SYBR Green Assay, Membrane