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Sangon Biotech te trisedta buffer
Te Trisedta Buffer, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/te+trisedta+buffer/buffer+te/pm41258095-264-7-17
Average 86 stars, based on 1 article reviews
te trisedta buffer - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Staining:

Article Title: De novo-designed ribozyme-controlled riboregulator for cell-free diagnostics.
Article Snippet: Flag-His tag protein detection test strips (cat. no. BF06219) were purchased from Biodragon. .. The T7 High Yield RNA Synthesis Kit (cat. no. B639253), TE buffer (pH 7.4) (cat. no. B548106), RNase-free ddH2O (cat. no. B541018), Acrylamide (cat. no. A601032), 10,000 × 4S Red Plus Nucleic Acid Stain (cat. no. A606695), 50 × Tris-acetate-EDTA (TAE) buffer (cat. no. B548101), and 5 × Tris-boric acidEDTA (TBE) buffer (cat. no. B548102) were all purchased from Sangon Biotech. .. The PrimeScriptTM FAST RT Kit (cat. no. RR092A) and TB Green® Premix Ex TaqTM II (Tli RNaseH AR TI CL E IN P RE SS Plus) Kit (cat. no. RR820A) were obtained from TaKaRa.

Incubation:

Article Title: Genetic Diversity Analysis of 96 Gossypium hirsutum - Gossypium barbadense Introgression Lines and Early Maturing Northern China Cotton Lines Using a 40K Liquid-Phase Chip.
Article Snippet: .. Briefly, leaf samples were ground in liquid nitrogen and incubated in 2% CTAB buffer containing 2% β-mercaptoethanol (Sangon Biotech, Shanghai, China) at 65 ◦C for 60 min. After extraction with chloroform-isoamyl alcohol (24:1) (Sangon Biotech, Shanghai, China) twice, DNA was precipitated with isopropanol (Sangon Biotech, Shanghai, China), washed twice with 75% ethanol (Sangon Biotech, Shanghai, China), and dissolved in TE buffer (Sangon Biotech, Shanghai, China). ..

Article Title: Genetic Diversity Analysis of 96 Gossypium hirsutum - Gossypium barbadense Introgression Lines and Early Maturing Northern China Cotton Lines Using a 40K Liquid-Phase Chip
Article Snippet: .. Briefly, leaf samples were ground in liquid nitrogen and incubated in 2% CTAB buffer containing 2% β-mercaptoethanol (Sangon Biotech, Shanghai, China) at 65 °C for 60 min. After extraction with chloroform-isoamyl alcohol (24:1) (Sangon Biotech, Shanghai, China) twice, DNA was precipitated with isopropanol (Sangon Biotech, Shanghai, China), washed twice with 75% ethanol (Sangon Biotech, Shanghai, China), and dissolved in TE buffer (Sangon Biotech, Shanghai, China). ..

Extraction:

Article Title: Genetic Diversity Analysis of 96 Gossypium hirsutum - Gossypium barbadense Introgression Lines and Early Maturing Northern China Cotton Lines Using a 40K Liquid-Phase Chip.
Article Snippet: .. Briefly, leaf samples were ground in liquid nitrogen and incubated in 2% CTAB buffer containing 2% β-mercaptoethanol (Sangon Biotech, Shanghai, China) at 65 ◦C for 60 min. After extraction with chloroform-isoamyl alcohol (24:1) (Sangon Biotech, Shanghai, China) twice, DNA was precipitated with isopropanol (Sangon Biotech, Shanghai, China), washed twice with 75% ethanol (Sangon Biotech, Shanghai, China), and dissolved in TE buffer (Sangon Biotech, Shanghai, China). ..

Article Title: Genetic Diversity Analysis of 96 Gossypium hirsutum - Gossypium barbadense Introgression Lines and Early Maturing Northern China Cotton Lines Using a 40K Liquid-Phase Chip
Article Snippet: .. Briefly, leaf samples were ground in liquid nitrogen and incubated in 2% CTAB buffer containing 2% β-mercaptoethanol (Sangon Biotech, Shanghai, China) at 65 °C for 60 min. After extraction with chloroform-isoamyl alcohol (24:1) (Sangon Biotech, Shanghai, China) twice, DNA was precipitated with isopropanol (Sangon Biotech, Shanghai, China), washed twice with 75% ethanol (Sangon Biotech, Shanghai, China), and dissolved in TE buffer (Sangon Biotech, Shanghai, China). ..

Saline:

Article Title: Comparative evaluation of ultrafiltration and EMSA formats for siRNA plasma protein binding: methodological determinants and artifacts
Article Snippet: E-GelTM Power 150 Snap Electrophoresis Device and E-GelTM EX Double Comb 2% agarose were purchased from 151 Thermo Fisher Scientific. .. Tris-EDTA (TE) Buffer was obtained from Sangon Biotech (Shanghai) 152 Co., Ltd. Tween 20 and BupHTM Phosphate Buffered Saline Packs was obtained from Sigma-153 Aldrich. .. Amicon® Ultra-15 (30 kDa MWCO) and Ultra-0.5 (50 kDa MWCO) Centrifugal filter 154 devices were both obtained from Millipore Sigma (Merck KGaA, Germany).

Article Title: Flexible regulation of CRISPR/Cas12a activity by spatial confinement effect
Article Snippet: .. HPLC-purified oligonucleotides , TE buffer, RNase inhibitor, 10 × phosphate-buffered saline (PBS), RNase-free water, and all DNase/RNase-free tips were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). .. 6 × loading buffer and 20 bp DNA Marker were obtained from Takara Biotech Inc. (Dalian, China).

Article Title: Modular CRISPR-Cas12a-Activated Gold Nanoparticle Assay for Rapid Visual Detection of Hepatocellular Carcinoma-Related miRNAs.
Article Snippet: ). he ABSTRACT: MicroRNAs (miRNAs) are emerging biomarkers for early hepatocellular carcinoma (HCC) detection, but most CRISPR−Cas12a assays rely on reverse transcription and preamplification and often lack simple visual readouts.. Here, we develop a reverse transcription-free, cleavage-guided strategy that converts miRNA recognition into visual outputs.. In the presence of the target miRNA, a scaffold RNA forms an active crRNA that triggers Cas12a trans-cleavage using a preformed dsDNA activator, enabling two readout formats.

High Performance Liquid Chromatography:

Article Title: Flexible regulation of CRISPR/Cas12a activity by spatial confinement effect
Article Snippet: .. HPLC-purified oligonucleotides , TE buffer, RNase inhibitor, 10 × phosphate-buffered saline (PBS), RNase-free water, and all DNase/RNase-free tips were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). .. 6 × loading buffer and 20 bp DNA Marker were obtained from Takara Biotech Inc. (Dalian, China).



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