te buffer (Thermo Fisher)

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Thermo Fisher te buffer
Te Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/te+buffer/TE+buffer%2C+pH+8%2E0%2C+RNAse+free/pmc13085942-115-41-45
Average 94 stars, based on 1 article reviews

te buffer - by Bioz Stars, 2026-11

94/100 stars

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Purification:

Article Title: Extracellular vesicles from wild-type Epstein–Barr virus–transformed B-cells export host DNA and the viral lncRNA EBER1
Article Snippet: EV DNA was isolated from extracellular vesicles using the Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Mini Kit (Norgen Biotek Corp, Thorold, ON, Canada). .. Prior to column purification, lysates were treated with RNase A (ThermoFisher Scientific, Carlsbad, CA, USA). .. DNA quality and size distribution were evaluated using TapeStation Genomic DNA and High Sensitivity D5000 assays (Agilent Technologies, Waldbronn, Germany).

Article Title: Wolbachia Induces Epigenetic and Transcriptional Modifications in the Orthopteran Chorthippus parallelus (Acrididae: Gomphocerinae).
Article Snippet: Briefly, the total RNA was extracted using 500 μL of TRIzol Reagent (Invitrogen, Life Technologies, Carlsbad, CA, USA), following the manufacturer’s protocol. .. RNA was subsequently treated with 1 μL of DNase I and 5 μL of the corresponding 10× DNase Buffer (Invitrogen, Life Technologies, Carlsbad, CA, USA) and further purified using 200 μL of phenol:chloroform alcohol (Fluka, Neu-Ulm, Germany), using 5PRIME Phase Lock Gel Light tubes (Quantabio, Beverly, MA, USA). .. Purified RNA was resuspended in nuclease-free water, quantified spectrophotometrically at 260 nm with a BioPhotometer® (Eppendorf, Hamburg, Germany), and stored at −80 ◦C.

Real-time Polymerase Chain Reaction:

Article Title: Optimising Vaginal Microbiome Profiling for Clinical Translation: A Comparative Assessment of Sample Storage Methods and a Vagina-Specific 16S rRNA Gene Database.
Article Snippet: .. The qPCR targeted the V4 hypervariable region of the 16S rRNA gene, using the broadly conserved primers recommended by the Earth Microbiome Project (EMP): 515F (5′-GTGCCAGCMGCCGCGGTAA-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3′) [41,42]. qPCR reactions were carried out in 25 μL volumes, containing: 2 mM MgCl2 (Applied Biosystems Inc. [ABI], Foster City, CA, USA), 4 U/μL AmpliTaq Gold DNA Polymerase (ABI), 1X Taq polymerase buffer (ABI), 0.4 μM dNTPs (Astral Scientific, Sydney, Australia), 0.1 mg/mL bovine serum albumin (BSA; Fisher Biotec, Perth, Australia), 0.2X SYBR Green (Invitrogen, Carlsbad, CA, USA), 0.4 μM of each primer (Integrated DNA Technologies [IDT], Melbourne, Australia), and 4 μL of DNA template (either undiluted or diluted 1:2). ..

SYBR Green Assay:

Synthesized:

Article Title: 2'-O-Methyl-guanosine RNA fragments antagonize TLR7 and TLR8 to limit autoimmunity.
Article Snippet: 2′-OMe guanosine, 2′-OMe adenosine, 2′-OMe uridine, 2′-OMe cytosine, guanosine, adenosine and cytosine were resuspended in DMSO and purchased from MedChemExpress for cell-based studies and LC-MS analyses as standards. .. Immunostimulatory ssRNA40PS (ref. 12), RNA9.2PS7 (ref. 12), B-406-AS-1 (ref. 37), ssRNA40PO and RNA9.2sPO ssRNAs, trimer and longer oligonucleotides were all commercially synthesized by Integrated DNA Technologies, Syngenis or Wuxi AppTec and resuspended in RNase-free TE buffer, pH 8.0 (Thermo Fisher Scientific). ..

Lysis:

Article Title: Differential DNA methylation clock ages across buffy coat (BC), peripheral blood mononuclear cells (PBMC), and saliva in individuals in early-to-mid adulthood
Article Snippet: .. Briefly, 600 μL of saliva in lysis buffer was sequentially treated with RNase A and proteinase K followed by precipitation with two volumes of isopropyl alcohol and pelleted, washed with 1 mL 70% ethanol, air-dried and resuspended in 110 μL of TE Buffer pH 8.0 (Invitrogen). ..

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