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te redissolving buffer  (MACHEREY NAGEL)


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    Structured Review

    MACHEREY NAGEL te redissolving buffer
    Te Redissolving Buffer, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/te+buffer/Redissolving+Buffer+TE%E2%80%91EF/pm37832537-644-23-26
    Average 91 stars, based on 2 article reviews
    te redissolving buffer - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Adhesion-based capture stabilizes nascent microvilli at epithelial cell junctions.
    Article Snippet: After centrifugation for 10 min at 11,000Xg, the supernatant was collected, and 600 mLmolecular grade isopropanol (Fisher Scientific Cat# BP2618500) was added and the tube was mixed by inverting. .. After pelleting by centrifugation for 5 min at 11,000Xg, the pellet was washed in 70% ethanol (Macherey Nagel) and resuspended in 25 mL TE Redissolving Buffer (Macherey Nagel Cat# 740797.1) and DNA concentration was measured on a NanoDrop. .. Genomic PCR A region of 610bp encompassing exons 4 and 5 of the porcine CDHR2 genomic sequence (see key resources table for primers) was generated by PCR following the Q5 High-Fidelity Polymerase (New England Biolabs Cat# M0491L) protocol with a genomic template of 200 ng from the clonal ‘‘KO’’ populations.

    Concentration Assay:

    Article Title: Adhesion-based capture stabilizes nascent microvilli at epithelial cell junctions.
    Article Snippet: After centrifugation for 10 min at 11,000Xg, the supernatant was collected, and 600 mLmolecular grade isopropanol (Fisher Scientific Cat# BP2618500) was added and the tube was mixed by inverting. .. After pelleting by centrifugation for 5 min at 11,000Xg, the pellet was washed in 70% ethanol (Macherey Nagel) and resuspended in 25 mL TE Redissolving Buffer (Macherey Nagel Cat# 740797.1) and DNA concentration was measured on a NanoDrop. .. Genomic PCR A region of 610bp encompassing exons 4 and 5 of the porcine CDHR2 genomic sequence (see key resources table for primers) was generated by PCR following the Q5 High-Fidelity Polymerase (New England Biolabs Cat# M0491L) protocol with a genomic template of 200 ng from the clonal ‘‘KO’’ populations.

    other:

    Article Title: Adhesion-based capture stabilizes nascent microvilli at epithelial cell junctions.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-PCLKC [CDHR2] (mouse) [1:25] Abnova Cat# H00054825-M01; RRID: AB_490042 anti-CDHR5 (rabbit) [1:250] Sigma-Aldrich Cat# HPA009173; RRID: AB_1079429 anti-GFP (chicken) [1:200] Aves Labs Cat# GFP-1020; RRID: AB_10000240 anti-ZO-1 (rat) [1:100] Millipore Cat# MABT11; RRID: AB_10616098 anti-ZO-1 (rabbit) [1:50] ThermoFisher Cat# 61-7300; RRID: AB_2533938 Goat anti-mouse Alexa Fluor 488 F(ab’)2 fragment [1:1000] ThermoFisher Cat# A-11017; RRID: AB_2534084 Goat anti-rabbit Alexa Fluor 488 F(ab’)2 fragment [1:1000] ThermoFisher Cat# A-11070; RRID: AB_2534114 Goat anti-rabbit Alexa Fluor 568 F(ab’)2 fragment [1:1000] ThermoFisher Cat# A-21069; RRID: AB_253573 Goat anti-chicken Alexa Fluor 488 IgG (H+L) [1:1000] ThermoFisher Cat# A-11039; RRID: AB_142924 Goat anti-rat Alexa Fluor 647 IgG (H+L) [1:200] ThermoFisher Cat# A-21247; RRID: AB_141778 Bacterial and virus strains E. coli DH5-Alpha competent cells Molecular Cell Biology Resource Core, Vanderbilt Medical Center Item# DH5 Alpha Chemicals, peptides, and recombinant proteins Alexa Fluor Plus 405 Phalloidin [1:200] ThermoFisher Cat# A30104 Alexa Fluor 647 Phalloidin [1:200] ThermoFisher Cat# A22287 Janelia Fluor 635 (JF635) Janelia N/A Paraformaldehyde, 16% Electron Microscopy Sciences Cat# 15710 Triton X-100 Sigma Cat# T8787 ProLong Gold Antifade Reagent Invitrogen Cat# P36930 FuGENE 6 Promega Cat# E2691 Lipofectamine 2000 ThermoFisher Cat# 11668019 Polybrene Infection Reagent Sigma-Aldrich Cat# TR-1003 G418 Sulfate Gold Biotechnology Cat#G-418-1 Puromycin Gold Biotechnology Cat# P-600-100 Antibiotic-antimycotic (anti-anti) Gibco Cat# 15240062 (-)-Blebbistatin Selleck Chemicals Cat# S7099 DMSO Sigma Cat# D8418 BsmBI-v2 enzyme New England Biolabs Cat# R0739 Q5 High-Fidelity DNA Polymerase New England Biolabs Cat# M0491L Q5 High GC Enhancer New England Biolabs Cat# B9028A Q5 Reaction Buffer New England Biolabs Cat# B9027S Deoxynucleotide Mix, 10mM Sigma-Aldrich Cat# D7295 EDTA, 0.5M Corning Cat# 46-034-Cl Nuclei Lysis Solution Promega Cat# A7941 Protein Precipitation Solution Promega Cat# A795A Proteinase K Sigma Cat# P4850 Molecular Biology Grade Isopropanol Fisher Scientific Cat# BP2618500 70% Ethanol Macherey-Nagel N/A TE-EF Redissolving Buffer Macherey-Nagel Cat# 740797.1 Glutaraldehyde, 25% Electron Microscopy Sciences Cat# 16220 Tannic Acid Electron Microscopy Sciences Cat# 21700 Osmium Tetroxide Electron Microscopy Sciences Cat# 19112 (Continued on next page) Developmental Cell 58, 2048–2062.e1–e7, October 23, 2023 e1



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