10x tbst (EZ Bioresearch)

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EZ Bioresearch 10x tbst

10x Tbst, supplied by EZ Bioresearch, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tbst+buffer/tbst+buffer/pmc08784773-36-0-3
Average 90 stars, based on 1 article reviews

10x tbst - by Bioz Stars, 2026-11

90/100 stars

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1) Product Images from "Pegylated arginine deiminase drives arginine turnover and systemic autophagy to dictate energy metabolism"

Article Title: Pegylated arginine deiminase drives arginine turnover and systemic autophagy to dictate energy metabolism

Journal: Cell Reports Medicine

doi: 10.1016/j.xcrm.2021.100498

Figure Legend Snippet:

Techniques Used: Virus, Plasmid Preparation, Recombinant, Injection, SYBR Green Assay, Staining, Western Blot, Enzyme-linked Immunosorbent Assay, Colorimetric Assay, Solvent, Reverse Transcription, Gene Expression, Software

Virus:

Article Title: Pegylated arginine deiminase drives arginine turnover and systemic autophagy to dictate energy metabolism.
Article Snippet: .. Reagent or resource Source Identifier Antibodies FGF21 Abcam Cat# ab171941; RRID:AB_2629460 b-ACTIN Cell Signaling Technology Cat# 3700S; RRID:AB_2242334 LC3B Novus Biologicals Cat# NB100-2220; RRID:AB_10003146 p62/SQSTM1 Abcam Cat# ab56416; RRID:AB_945626 GHRL Santa Cruz Cat# sc-517596; RRID: NA phospho-mTOR (Ser2448) Cell Signaling Technology Cat# 5536S; RRID:AB_10691552 mTOR Cell Signaling Technology Cat# 2983S; RRID:AB_2105622 phospho-ULK1 (Ser757) Cell Signaling Technology Cat# 14202S; RRID:AB_2665508 ULK1 Cell Signaling Technology Cat# 8054S; RRID:AB_11178668 phospho-p70 S6 Kinase (Thr389) Cell Signaling Technology Cat# 9234S; RRID:AB_2269803 p70 S6 Kinase Cell Signaling Technology Cat# 2708S; RRID:AB_390722 phospho-4E-BP1 (Thr37/46) Cell Signaling Technology Cat# 2855S; RRID:AB_560835 4E-BP1 Cell Signaling Technology Cat# 9644S; RRID:AB_2097841 Horse Anti-Mouse IgG-HRP Cell Signaling Technology Cat# 7076S; RRID:AB_330924 Goat Anti-Rabbit IgG-HRP Cell Signaling Technology Cat# 7074S; RRID:AB_2099233 Bacterial and virus strains AAV8-eGFP Vector Biolabs Cat# 1060 AAV8-TGB-arcA Vector Biolabs NA Ad-CMV-eGFP Vector Biolabs NA Ad-CMV-arcA-eGFP Vector Biolabs NA Biological samples Fetal Bovine Serum (FBS) GIBCO Cat# 26140-079 Chemicals, peptides, and recombinant proteins ADI-PEG 20 Polaris Pharmaceuticals Inc. Kit# 36386 TRIzol Invitrogen Cat# 15596018 0.9% Sodium Chloride Injection, USP Hospira Fast SYBR Green Master Mix Applied Biosystems Cat# 4385612 Pierce Protease and Phosphatase Inhibitor Mini Tablets, EDTA-Free Thermo Scientific Cat# A32961 10X TBST EZ BioResearch Cat# S-1012 10X Tris/Glycine Buffer Bio-Rad Cat# 1610734 10X Tris/Glycine/SDS Buffer Bio-Rad Cat# 1610732 Mini-PROTEAN TGX Stain-Free Gels Bio-Rad Cat# 4568094 Clarity Western ECL Substrate Bio-Rad Cat# 1705060 Humulin R Lilly USA, LLC NDC 0002-8215-17 Ultra Sensitive Mouse Insulin ELISA Kit Crystal Chem Cat# 90080 Mouse/Rat Fibroblast Growth Factor 21 ELISA BioVendor Cat# RD291108200R Glucose Colorimetric Assay Kit Cayman Chemical Cat# 10009582 Triglycerides Standard Pointe Scientific Cat# T7531-STD Infinity Triglycerides Thermo Scientific Cat# TR22421 Cholesterol Standard Stanbio Cat# 1012-030 Infinity Cholesterol Thermo Scientific Cat# TR13421 NEFA Standard Solution Fujifilm Cat# 276-76491 (Continued on next page) Cell Reports Medicine 3, 100498, January 18, 2022 e1 .. Reagent or resource Source Identifier HR Series NEFA-HR(2) Color Reagent A Fujifilm Cat# 999-34691 HR Series NEFA-HR(2) Solvent A Fujifilm Cat# 995-34791 HR Series NEFA-HR(2) Color Reagent B Fujifilm Cat# 991-34891 HR Series NEFA-HR(2) Solvent B Fujifilm Cat# 993-35191 HDL-C/LDL-C Calibrator Fujifilm Cat# 990-28011 L-Type LDL-C Reagent 1 Fujifilm Cat# 993-00404 L-Type LDL-C Reagent 2 Fujifilm Cat# 999-00504 Albumin from Bovine Serum Sigma-Aldrich SKU A3983-50G 2-Propanol Sigma-Aldrich SKU 190764-500ML Methanol Sigma-Aldrich SKU 179337-4L-PB Chloroform Sigma-Aldrich SKU 319988-500ML Ethanol Decon Laboratories, Inc. Cat# 2701 D-(+)-Glucose Sigma-Aldrich SKU G8270-100G DMEM/F12(1:1) (1X) GIBCO Cat# 11330-032 Insulin-Transferrin-Selenium 100X GIBCO Cat# 41400-045 Dexamethasone Sigma-Aldrich SKU D4902-100MG Pen Strep GIBCO Cat# 15140-122 Rodent Diet: Adjusted Calories Diet (42% from fat) Envigo Teklad Diets Cat# TD.88137 Nuclease-Free Water Invitrogen Cat# AM9937 Critical commercial assays Seahorse XF Cell Mito Stress Test Kit Agilent Cat# 103015-100 QuantiTect Reverse Transcription Kit QIAGEN Cat# 205314 Deposited data Gene Expression RNA-seq data This paper GEO: GSE191295 Single-cell ATAC-seq data This paper GEO: GSE192413 Experimental models: Cell lines Mouse: AML12 ATCC Cat# CRL-2254; RRID:CVCL_0140 Experimental models: Organisms/strains Mouse: C57B/J6 Jackson Laboratory RRID: IMSR_JAX:000664 Mouse: db/db Jackson Laboratory RRID: IMSR_JAX:000642 Mouse: Becn1+/ Jackson Laboratory RRID: IMSR_JAX:018429 Mouse: Becn1 flox Jackson Laboratory RRID: IMSR_JAX:028794 Mouse: Alb1-cre Jackson Laboratory RRID: IMSR_JAX:016832 Mouse: Fgf21 flox Jackson Laboratory RRID: IMSR_JAX:022361 Oligonucleotides qPCR Primers This paper Table S1 Software and algorithms GraphPad Prism 7 GraphPad Software Inc. http://www.graphpad.com; RRID:SCR_002798 ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/; RRID:SCR_003070

Recombinant:

Injection:

SYBR Green Assay:

Staining:

Western Blot:

Enzyme-linked Immunosorbent Assay:

Colorimetric Assay:

other:

Article Title: Mutational scanning of CRX classifies clinical variants and reveals biochemical properties of the transcriptional effector domain.
Article Snippet: TBST (EZ BioResearch 681 #S-1012, diluted to 1X in deionized water) and EveryBlot Blocking Buffer (Bio-Rad 682 #12010020) were used for washing and blocking, respectively.

Article Title: Mutational scanning of CRX classifies clinical variants and reveals biochemical properties of the transcriptional effector domain
Article Snippet: TBST (EZ BioResearch S-1012, diluted to 1× in deionized water) and EveryBlot Blocking Buffer (Bio-Rad 12010020) were used for washing and blocking, respectively.

Membrane:

Article Title: Labor induction with oxytocin in pregnant rats is not associated with oxidative stress in the fetal brain.
Article Snippet: .. The membrane was subsequently blocked with TBST buffer (catalog # S1012, EZ Bioresearch) containing 5% milk for 1 h at room temperature on a shaker. .. Following a brief wash with TBST buffer, the membrane was immunoblotted overnight at 4 °C on a shaker with primary rabbit anti-OxtR antibody (catalog # TA351476, OriGene Technologies), at a dilution of 1:250 in 5% milk-TBST buffer.

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