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Solarbio Inc tris-buffered saline-tween (tbst
Tris Buffered Saline Tween (Tbst, supplied by Solarbio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tbst+buffer/tris+buffered+saline+tween+20++tbst/pmc07746446-51-15-18
Average 90 stars, based on 1 article reviews
tris-buffered saline-tween (tbst - by Bioz Stars, 2026-11
90/100 stars

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Article Title: Regulation of macrophage polarization and glucose metabolism by the ERK/MAPK-HK1 signaling pathway in paraquat-induced acute lung injury.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Article Title: Exosomal miR-192-5p secreted by bone marrow mesenchymal stem cells inhibits hepatic stellate cell activation and targets PPP2R3A.
Article Snippet: Bone marrow mesenchymal stem cell (BSMC)-derived extracellular vehicles (EVs) have a pivotal therapeutic potential in hepatic fibrosis (HF).. Activation of hepatic stellate cells (HSCs) is the key mechanism in HF progression.. Downregulation of miR-192-5p was previously observed in activated HSCs.

Article Title: The Degree of Helicobacter pylori Infection Affects the State of Macrophage Polarization through Crosstalk between ROS and HIF-1 α
Article Snippet: After incubation with primary antibodies at 4°C overnight, the membranes were washed three times with Tris-buffered saline-Tween (TBST) (Solarbio Biotechnology) and then incubated with a horseradish peroxidase-conjugated secondary antibody (Zhongshan Golden Bridge Biotech; dilution 1 : 5,000) for 1 h at room temperature.

Article Title: Region-specific sympatho-adrenergic regulation of specialized vasculature in bone homeostasis and regeneration
Article Snippet: Tris-buffered saline-Tween (TBS-T) , Solarbio , Cat# T1081-10.

Article Title: Baicalin attenuates dexamethasone-induced apoptosis of bone marrow mesenchymal stem cells by activating the hedgehog signaling pathway
Article Snippet: Subsequently, those membranes were placed in tris-buffered saline with Tween 20 (TBST) (Solarbio) containing 5% skimmed milk powder for 1.5 hours.

Staining:

Article Title: Exploring a blue-light-sensing transcription factor to double the peak productivity of oil in Nannochloropsis oceanica
Article Snippet: .. Specifically, below was inserted into Results (Line 235-237): “The 280 NobZIP77:gfp fusion protein was colocalized with the DAPI-stained nuclei, indicating 281 nucleus targeting (Fig. 4A).” 282 283 Moreover, below was inserted into Methods (Line 639-643): “To validate the nucleus- 284 localization of NobZIP77, the NobZIP77:GFP line and the WT strain were stained with 0.5 285 μg/mL 4',6-diamidino-2-phenylindole (DAPI, Solarbio) for 5 min at room temperature and 286 then twice-washed with Tris buffered saline Tween (TBST, Solarbio) for 4 min at room 287 temperature. ..

Saline:

Article Title: Exploring a blue-light-sensing transcription factor to double the peak productivity of oil in Nannochloropsis oceanica
Article Snippet: .. Specifically, below was inserted into Results (Line 235-237): “The 280 NobZIP77:gfp fusion protein was colocalized with the DAPI-stained nuclei, indicating 281 nucleus targeting (Fig. 4A).” 282 283 Moreover, below was inserted into Methods (Line 639-643): “To validate the nucleus- 284 localization of NobZIP77, the NobZIP77:GFP line and the WT strain were stained with 0.5 285 μg/mL 4',6-diamidino-2-phenylindole (DAPI, Solarbio) for 5 min at room temperature and 286 then twice-washed with Tris buffered saline Tween (TBST, Solarbio) for 4 min at room 287 temperature. ..

Article Title: Interaction between the VP2 protein of deformed wing virus and host snapin protein and its effect on viral replication
Article Snippet: After 24 h, the cells were fixed in 2 ml of 4% paraformaldehyde (Solarbio, Beijing, China) at 4°C for 20 min and then washed three times with PBS. .. Next, the cells were permeabilized with 2 ml of 0.5% Triton X-100 (Sangon Biotech, Shanghai, China) for 30 min at room temperature, washed three times with PBS, and then blocked in Tris-buffered saline-Tween 20 (TBST) containing 2% bovine serum albumin (Solarbio) for 1 h at room temperature. .. The coverslips were incubated overnight together with His-tagged and Flag-tagged antibodies (1:400 dilution; Proteintech) at 4°C.

Binding Assay:

Article Title: Excessive Lipid Peroxidation in Uterine Epithelium Causes Implantation Failure and Pregnancy Loss
Article Snippet: .. All slides were performed with heat‐induced epitope retrieval in Tris‐EDTA buffer (pH9.0) for 15 min, then washed with 1× TBST buffer (Solarbio, T1082) and blocked non‐specific binding with 30% goat serum for 1 h. According to the manufacturer's instructions, primary antibodies were diluted with Opal Antibody Diluent (ARD1001EA) and applied at 4 °C overnight. ..



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