non specific antigens (MedChemExpress)

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MedChemExpress non specific antigens
Non Specific Antigens, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tbs+t+buffer/Rapid+Blocking+Buffer+(TBS-T)+Powder/pmc13065056-72-2-9
Average 94 stars, based on 13 article reviews

non specific antigens - by Bioz Stars, 2026-11

94/100 stars

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Article Title: Erianin protects chondrocytes against IL-1β-induced oxidative stress and ferroptosis by activating GPX4/STING signaling in osteoarthritis.
Article Snippet: 30 mg of cellular proteins were transferred onto a polyvinylidene fluoride (PVDF) membrane (Bio-Rad, United States) via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).

Membrane:

Article Title: Erianin protects chondrocytes against IL-1β-induced oxidative stress and ferroptosis by activating GPX4/STING signaling in osteoarthritis
Article Snippet: .. After washing with TBST (Cat. No.: HY-K1027, MedChemExpress, USA) three times, the membrane was incubated with the secondary antibody for 1 h at room temperature. .. The protein blots were then detected using an ultra-sensitive ECL chemiluminescence kit (Beyotime, China).

Incubation:

Article Title: Targeting SKAP2 restores sperm motility and morphology through modulating mitochondrial organization and cytoskeletal remodeling
Article Snippet: Proteins were then transferred onto PVDF membranes (Bio-Rad) via electroblotting. .. Membranes were blocked with Rapid Blocking Buffer (TBS-T) Powder (100 mL of 1×) from MedChemExpress (Monmouth Junction, NJ, USA; Cat# HY-K1027) and incubated with primary antibodies, followed by HRP-conjugated secondary antibodies for 1–2 h at room temperature. .. Signals were visualized using Sensitive ECL Kit from MedChemExpress (Monmouth Junction, NJ, USA; Cat# HY-K2006) and captured with the ChemiDoc XRS+ imaging system (Bio-Rad).

Article Title: Multiparity exacerbates Aβ accumulation and promotes cellular senescence in a mouse model of amyloidosis.
Article Snippet: The proteins were then transferred to a PVDF membrane using the Trans-blot Turbo Transfer system (Bio-Rad). .. Following transfer, membranes were incubated with rapid blocking buffer (MCE, HY-K1027) for 15 min at room temperature. ..

Blocking Assay:

Article Title: JMJD3 regulates the M2-like macrophage polarization and promotes the growth of breast cancer cells via STAT6/IRF4 axis
Article Snippet: Proteins (20 μg per sample) were separated by SDS-PAGE (Whatman, Maidstone, Kent, UK) and transferred to a PVDF membrane. .. After blocking non-specific antigens with quick blocking buffer (HY-K1027; MCE), the membranes were incubated overnight at 4°C with primary antibodies: Anti-JMJD3 (1:2000) (PA5−72751; Thermo Fisher Scientific, New York, USA), Anti-Histone H3 (tri methl K27) (1:1000) (ab6002; Abcam, Cambridge, UK), Anti-IRF4 (1:1000) (ab133590; Abcam), Anti-CD206 (1:1000) (MA5−32498; Thermo Fisher Scientific), Anti-STAT6 (phospho Y641) (1:2000) (ab28829; Abcam), Anti-Arg1 (1:500) (14-9779-82; Thermo Fisher Scientific), GAPDH (1:5000) (R1108-1; HUABIO, Hangzhou, China), β -actin (1:10000) (ET1702−52; HUABIO). .. ECL-Plus (Thermo Fisher Scientific, New York, USA) and secondary HRP-conjugated antibodies were used to visualize proteins.

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