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buffer  (Thermo Fisher)


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Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
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  • 95

    Structured Review

    Thermo Fisher buffer
    Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tbe+buffer/TBE+urea+sample+buffer/pm42235517-900-18-19
    Average 95 stars, based on 1 article reviews
    buffer - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Staining:

    Article Title: Structural basis for protein-free catalysis by ribonuclease P ribozyme.
    Article Snippet: .. Samples were analyzed on an 8 M urea Tris-borate-EDTA (TBE) denaturing preparative 8% polyacrylamide (29:1 acrylamide: bis-acrylamide) gel and stained with SYBRTM Gold (Invitrogen Thermo Fisher Scientific). ..

    Article Title: The E. coli DEAD box ATPase CsdA, is a NAD + capped RNA binding protein
    Article Snippet: RNA was extracted post DNase treatment using acid phenol: chloroform: isoamyl alcohol, 25:24:1 (Sigma Aldrich) and recovered by ethanol precipitation. .. The RNAs were resuspended in RNase free water post recovery, and purity confirmed by TBE Urea PAGE gel electrophoresis on a 10% TBE Urea gel (Thermo fisher Scientific) and visualized by SYBR Gold staining (Thermo Fisher Scientific). .. In vitro synthesized single stranded probe RNA was subjected to 3′ biotinylation using pCp-Biotin (Jena Bioscience) in presence of T4 RNA ligase 1 (New England Biolabs).

    Polyacrylamide Gel Electrophoresis:

    Article Title: The E. coli DEAD box ATPase CsdA, is a NAD + capped RNA binding protein
    Article Snippet: RNA was extracted post DNase treatment using acid phenol: chloroform: isoamyl alcohol, 25:24:1 (Sigma Aldrich) and recovered by ethanol precipitation. .. The RNAs were resuspended in RNase free water post recovery, and purity confirmed by TBE Urea PAGE gel electrophoresis on a 10% TBE Urea gel (Thermo fisher Scientific) and visualized by SYBR Gold staining (Thermo Fisher Scientific). .. In vitro synthesized single stranded probe RNA was subjected to 3′ biotinylation using pCp-Biotin (Jena Bioscience) in presence of T4 RNA ligase 1 (New England Biolabs).

    Nucleic Acid Electrophoresis:

    Article Title: The E. coli DEAD box ATPase CsdA, is a NAD + capped RNA binding protein
    Article Snippet: RNA was extracted post DNase treatment using acid phenol: chloroform: isoamyl alcohol, 25:24:1 (Sigma Aldrich) and recovered by ethanol precipitation. .. The RNAs were resuspended in RNase free water post recovery, and purity confirmed by TBE Urea PAGE gel electrophoresis on a 10% TBE Urea gel (Thermo fisher Scientific) and visualized by SYBR Gold staining (Thermo Fisher Scientific). .. In vitro synthesized single stranded probe RNA was subjected to 3′ biotinylation using pCp-Biotin (Jena Bioscience) in presence of T4 RNA ligase 1 (New England Biolabs).

    other:

    Article Title: Systematic identification of variant-specific RNA structure-small molecule interactions exemplified by RNA G-quadruplexes.
    Article Snippet: For the index PCR, a 25μL reaction containing amplicon PCR product, 0.5 μL of Platinum SuperFi II DNA Polymerase, 1× SuperFi II Buffer (Thermo Fisher Scientific), and 0.5μM each of Nextera XT Index Kit v2 index primers (Illumina) was prepared.

    Purification:

    Article Title: DMN-seq enriches DNA hypomethylated regions for biomarker discovery using 5-methylcytosine glycosylase.
    Article Snippet: The final gel filtration was performed on a HiLoad 16/600 Superdex 200-pg column (GE Healthcare). .. 100ng of 82bp dsDNA 5mC oligo and 82bp dsDNA 5hmC oligo (IDT, Additional file 1: Table S1), respectively, were treated in 1x DME reaction buffer (80 mM Tris-HCl, pH 8, 200 mM NaCl, 200 mM EDTA, 1 mM dithiothreitol (DTT), 400 μg/mL BSA) and a reaction volume of 30 μl with 0, 125, 250, 500, 750 and 1000 ng of DME, and the treatment reaction was carried out at 37 °C for 2 h. After purification with a DNA Clean & Concentrate kit (Zymo Research), the oligos were eluted in 20 μl of water and mixed with 20 μl of Novex 2x Tris-borate-EDTA (TBE)-Urea Sample Buffer (Invitrogen) to run on 10% Novex TBE-Urea Gel (Invitrogen) at constant 180 V for 80 min. .. The gel was stained with 2 μl of SYBR Gold Nucleic Acid Gel Stain (Invitrogen) in 25 ml of TBE buffer at room temperature for 15 min.

    Luciferase:

    Article Title: Historical Pandemic and Contemporary Influenza A Viruses Reveal PB2 M631L as a Convergent Adaptation to Human ANP32
    Article Snippet: .. Equal amounts of cell lysates from luciferase assay replicates were pooled; denatured in reducing SDS-PAGE sample buffer for 5 min at 95 °C; separated on 8% SDS gels; subjected to immunoblotting with antibodies against PB2 (rabbit, ThermoFisher/Pierce, Dreieich, Germany, #PAS-32221, used at 1:2000 dilution), PB1 (rabbit, ThermoFisher/Pierce, Dreieich, Germany, # PAS34914 , 1:1000), PA (rabbit, GeneTex, Freising, Germany, #125932, 1:1000), NP (mouse, Acris, Herford, Germany, #AM00929PU-N, 1:2000) and actin (mouse, Sigma-Aldrich, St. Louis, MO, USA, #A2228, 1:5000), respectively and detected with HRP-labeled anti-mouse (DAKO, Glostrup, Denmark, #P0260) or anti-rabbit antibodies (DAKO, Glostrup, Denmark, #P0217), respectively. ..

    SDS Page:

    Article Title: Historical Pandemic and Contemporary Influenza A Viruses Reveal PB2 M631L as a Convergent Adaptation to Human ANP32
    Article Snippet: .. Equal amounts of cell lysates from luciferase assay replicates were pooled; denatured in reducing SDS-PAGE sample buffer for 5 min at 95 °C; separated on 8% SDS gels; subjected to immunoblotting with antibodies against PB2 (rabbit, ThermoFisher/Pierce, Dreieich, Germany, #PAS-32221, used at 1:2000 dilution), PB1 (rabbit, ThermoFisher/Pierce, Dreieich, Germany, # PAS34914 , 1:1000), PA (rabbit, GeneTex, Freising, Germany, #125932, 1:1000), NP (mouse, Acris, Herford, Germany, #AM00929PU-N, 1:2000) and actin (mouse, Sigma-Aldrich, St. Louis, MO, USA, #A2228, 1:5000), respectively and detected with HRP-labeled anti-mouse (DAKO, Glostrup, Denmark, #P0260) or anti-rabbit antibodies (DAKO, Glostrup, Denmark, #P0217), respectively. ..

    Western Blot:

    Article Title: Historical Pandemic and Contemporary Influenza A Viruses Reveal PB2 M631L as a Convergent Adaptation to Human ANP32
    Article Snippet: .. Equal amounts of cell lysates from luciferase assay replicates were pooled; denatured in reducing SDS-PAGE sample buffer for 5 min at 95 °C; separated on 8% SDS gels; subjected to immunoblotting with antibodies against PB2 (rabbit, ThermoFisher/Pierce, Dreieich, Germany, #PAS-32221, used at 1:2000 dilution), PB1 (rabbit, ThermoFisher/Pierce, Dreieich, Germany, # PAS34914 , 1:1000), PA (rabbit, GeneTex, Freising, Germany, #125932, 1:1000), NP (mouse, Acris, Herford, Germany, #AM00929PU-N, 1:2000) and actin (mouse, Sigma-Aldrich, St. Louis, MO, USA, #A2228, 1:5000), respectively and detected with HRP-labeled anti-mouse (DAKO, Glostrup, Denmark, #P0260) or anti-rabbit antibodies (DAKO, Glostrup, Denmark, #P0217), respectively. ..



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