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tangential flow filtration tff system  (Sartorius AG)


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    Sartorius AG tangential flow filtration tff system
    Tangential Flow Filtration Tff System, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tangential-flow+filtration/Vivaflow+TFF+Cassette+PES/pmc12376549-241-8-20
    Average 94 stars, based on 31 article reviews
    tangential flow filtration tff system - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Toward food-grade production of the Glutamicibacter halophytocola diamine oxidase using Komagataella phaffii.
    Article Snippet: Subsequently, DAO-GH 309 (523 mL) was partially purified by HIC (column volume of 350 mL) using the Toyopearl 310 Phenyl-650 M resin (Tosoh Bioscience, Tokyo, Japan). .. 311 312 Concentration of K. phaffii culture supernatant using cross-flow filtration 313 The K. phaffii culture supernatant obtained after secretory DAO-GH production with 314 the antibiotic-resistance-free clone was concentrated using a Vivaflow® 200 laboratory 315 cross-flow cassette (Sartorius AG, Göttingen, Germany) equipped with a 316 polyethersulfone membrane, which had a 10 kDa molecular weight cut-off. .. Subsequently, DAO-GH 309 (523 mL) was partially purified by HIC (column volume of 350 mL) using the Toyopearl 310 Phenyl-650 M resin (Tosoh Bioscience, Tokyo, Japan).

    Article Title: Microbiome as a Tool to Monitor Aquarium Systems
    Article Snippet: .. Concentration of Nucleic Acids from Water Samples To optimize the DNA yield, water samples were concentrated using a tangential flow filtration (TFF) system under negative pressure produced by a Masterflex Easy-loadTM pump (Thermo Fisher Scientific Inc., Waltham, MA, USA), and the water was flowed through a VivaflowTM 50 tangential flow cassette (Sartorius AG, Goettingen, Germany). ..

    Filtration:

    Article Title: Toward food-grade production of the Glutamicibacter halophytocola diamine oxidase using Komagataella phaffii.
    Article Snippet: Subsequently, DAO-GH 309 (523 mL) was partially purified by HIC (column volume of 350 mL) using the Toyopearl 310 Phenyl-650 M resin (Tosoh Bioscience, Tokyo, Japan). .. 311 312 Concentration of K. phaffii culture supernatant using cross-flow filtration 313 The K. phaffii culture supernatant obtained after secretory DAO-GH production with 314 the antibiotic-resistance-free clone was concentrated using a Vivaflow® 200 laboratory 315 cross-flow cassette (Sartorius AG, Göttingen, Germany) equipped with a 316 polyethersulfone membrane, which had a 10 kDa molecular weight cut-off. .. Subsequently, DAO-GH 309 (523 mL) was partially purified by HIC (column volume of 350 mL) using the Toyopearl 310 Phenyl-650 M resin (Tosoh Bioscience, Tokyo, Japan).

    Article Title: Limited consensus of marine viral diversity observed across techniques.
    Article Snippet: .. The seawater sample was filtered through a 0.45 μm and a 0.2 μm polyethersulfone (PES) membrane filter, and the viruses present in the samples were concentrated using tangential flow filtration with six 30 kDa PES Vivaflow 200 membranes (ref. VF20P0, Sartorius, Germany) connected in two parallel groups of three Vivaflow units in series. .. Next, the samples were filtered again through a 0.22 μm PES syringe membrane and stored at 4 °C until the next day, when the purified viral fractions were further concentrated to 250 μL using Amicon Ultra-15 mL centrifugal filter units (ref. UFC901024D, Merck Millipore, Massachusetts, USA).

    Article Title: Limited consensus of marine viral diversity observed across techniques
    Article Snippet: .. The seawater sample was filtered through a 0.45 μm and a 0.2 μm polyethersulfone (PES) membrane filter, and the viruses present in the samples were concentrated using tangential flow filtration with six 30 kDa PES Vivaflow 200 membranes (ref. VF20P0, Sartorius, Germany) connected in two parallel groups of three Vivaflow units in series. .. Next, the samples were filtered again through a 0.22 μm PES syringe membrane and stored at 4 °C until the next day, when the purified viral fractions were further concentrated to 250 μL using Amicon Ultra-15 mL centrifugal filter units (ref. UFC901024D, Merck Millipore, Massachusetts, USA).

    Article Title: Differential translocation of bacteriophages across the intestinal barrier in health and Crohn's disease.
    Article Snippet: McMurdie and Holmes 83 https://www.bioconductor.org/packages/ release/bioc/html/phyloseq.html Samtools 1.9 Li et al. 84 https://www.htslib.org/ SPAdes 3.14.0 Bankevich et al. 85 https://github.com/ablab/spades vContact2 0.9.19 Jang et al. 86 https://bitbucket.org/MAVERICLab/vcontact2 VIBRANT 1.2.1 Kieft et al. 87 https://github.com/AnantharamanLab/ VIBRANT VirSorter2 2.2.3 Guo et al. 88 https://github.com/jiarong/VirSorter2 Cell Reports 45, 116726, January 27, 2026 19 incubation and lysis of the bacteria by the phages, remaining bacteria and debris were removed by centrifugation for 30 min at 3,000 × g, and the supernatant filtered sequentially through 0.45-μm and 0.2-μm cellulose acetate filters (VWR Avantor). .. Phages were concentrated by tangential flow filtration using a Vivaflow cassette system (Sartorius Stedim Biotech) and stored in a final solution of salt-magnesium (SM) buffer (17 mM MgSO 4 ⋅7H 2 O, 100 mM NaCl, 5 mM Tris-HCl (pH 7.5) at 4 ◦ C. Lysates from the same bacteria cultured without phages were used as a mock negative control and were generated through a series of freeze/thaw cycles followed by sonication to closely mimic phage-induced lysis. ..

    Article Title: Microbiome as a Tool to Monitor Aquarium Systems
    Article Snippet: .. Concentration of Nucleic Acids from Water Samples To optimize the DNA yield, water samples were concentrated using a tangential flow filtration (TFF) system under negative pressure produced by a Masterflex Easy-loadTM pump (Thermo Fisher Scientific Inc., Waltham, MA, USA), and the water was flowed through a VivaflowTM 50 tangential flow cassette (Sartorius AG, Goettingen, Germany). ..

    Article Title: Nanoplastic concentration and potential transport in the Arctic Ocean
    Article Snippet: .. The pretreatment process started with ultrafiltering samples using an Amicon® Stirred Cell (500mL, Millipore) through 150 kDa (~12.7 nm in hydrodynamic diameter) poly(ether sulfone)filtrationmembranes (UP150, 1120871, Sterlitech Co.) or a Vivaflow® Tangential Flow Filtration Cassette (50 kDa, ~7.2 nm, 14558321, Sartorius Inc.) to concentrate nanoplastics in theon-board laboratory (Fig. S2).Themembranewas rinsedwith5%HCl to remove potential organic interference before use. ..

    Membrane:

    Article Title: Toward food-grade production of the Glutamicibacter halophytocola diamine oxidase using Komagataella phaffii.
    Article Snippet: Subsequently, DAO-GH 309 (523 mL) was partially purified by HIC (column volume of 350 mL) using the Toyopearl 310 Phenyl-650 M resin (Tosoh Bioscience, Tokyo, Japan). .. 311 312 Concentration of K. phaffii culture supernatant using cross-flow filtration 313 The K. phaffii culture supernatant obtained after secretory DAO-GH production with 314 the antibiotic-resistance-free clone was concentrated using a Vivaflow® 200 laboratory 315 cross-flow cassette (Sartorius AG, Göttingen, Germany) equipped with a 316 polyethersulfone membrane, which had a 10 kDa molecular weight cut-off. .. Subsequently, DAO-GH 309 (523 mL) was partially purified by HIC (column volume of 350 mL) using the Toyopearl 310 Phenyl-650 M resin (Tosoh Bioscience, Tokyo, Japan).

    Article Title: Limited consensus of marine viral diversity observed across techniques.
    Article Snippet: .. The seawater sample was filtered through a 0.45 μm and a 0.2 μm polyethersulfone (PES) membrane filter, and the viruses present in the samples were concentrated using tangential flow filtration with six 30 kDa PES Vivaflow 200 membranes (ref. VF20P0, Sartorius, Germany) connected in two parallel groups of three Vivaflow units in series. .. Next, the samples were filtered again through a 0.22 μm PES syringe membrane and stored at 4 °C until the next day, when the purified viral fractions were further concentrated to 250 μL using Amicon Ultra-15 mL centrifugal filter units (ref. UFC901024D, Merck Millipore, Massachusetts, USA).

    Article Title: Limited consensus of marine viral diversity observed across techniques
    Article Snippet: .. The seawater sample was filtered through a 0.45 μm and a 0.2 μm polyethersulfone (PES) membrane filter, and the viruses present in the samples were concentrated using tangential flow filtration with six 30 kDa PES Vivaflow 200 membranes (ref. VF20P0, Sartorius, Germany) connected in two parallel groups of three Vivaflow units in series. .. Next, the samples were filtered again through a 0.22 μm PES syringe membrane and stored at 4 °C until the next day, when the purified viral fractions were further concentrated to 250 μL using Amicon Ultra-15 mL centrifugal filter units (ref. UFC901024D, Merck Millipore, Massachusetts, USA).

    Article Title: Probiotic-inspired hybrid nanovesicles for enhancing immune checkpoint therapy efficiency via tumor immune microenvironment modulation
    Article Snippet: .. The filtrate was concentrated using a 10,000-molecular-weight-cutoff (MWCO) polyethersulphone (PES) membrane (Sartorius, Göttingen, Germany; catalog no. VF20P0) and the concentrated medium further centrifuged at 120,000 g at 4 °C for 2 h. Then, the precipitate was resuspended in PBS (pH 7.4). .. Micro BCA Protein Assay Kit (Thermo Fisher Scientific) was used to test the protein concentration of OMV solution.

    Molecular Weight:

    Article Title: Toward food-grade production of the Glutamicibacter halophytocola diamine oxidase using Komagataella phaffii.
    Article Snippet: Subsequently, DAO-GH 309 (523 mL) was partially purified by HIC (column volume of 350 mL) using the Toyopearl 310 Phenyl-650 M resin (Tosoh Bioscience, Tokyo, Japan). .. 311 312 Concentration of K. phaffii culture supernatant using cross-flow filtration 313 The K. phaffii culture supernatant obtained after secretory DAO-GH production with 314 the antibiotic-resistance-free clone was concentrated using a Vivaflow® 200 laboratory 315 cross-flow cassette (Sartorius AG, Göttingen, Germany) equipped with a 316 polyethersulfone membrane, which had a 10 kDa molecular weight cut-off. .. Subsequently, DAO-GH 309 (523 mL) was partially purified by HIC (column volume of 350 mL) using the Toyopearl 310 Phenyl-650 M resin (Tosoh Bioscience, Tokyo, Japan).

    Bacteria:

    Article Title: Differential translocation of bacteriophages across the intestinal barrier in health and Crohn's disease.
    Article Snippet: McMurdie and Holmes 83 https://www.bioconductor.org/packages/ release/bioc/html/phyloseq.html Samtools 1.9 Li et al. 84 https://www.htslib.org/ SPAdes 3.14.0 Bankevich et al. 85 https://github.com/ablab/spades vContact2 0.9.19 Jang et al. 86 https://bitbucket.org/MAVERICLab/vcontact2 VIBRANT 1.2.1 Kieft et al. 87 https://github.com/AnantharamanLab/ VIBRANT VirSorter2 2.2.3 Guo et al. 88 https://github.com/jiarong/VirSorter2 Cell Reports 45, 116726, January 27, 2026 19 incubation and lysis of the bacteria by the phages, remaining bacteria and debris were removed by centrifugation for 30 min at 3,000 × g, and the supernatant filtered sequentially through 0.45-μm and 0.2-μm cellulose acetate filters (VWR Avantor). .. Phages were concentrated by tangential flow filtration using a Vivaflow cassette system (Sartorius Stedim Biotech) and stored in a final solution of salt-magnesium (SM) buffer (17 mM MgSO 4 ⋅7H 2 O, 100 mM NaCl, 5 mM Tris-HCl (pH 7.5) at 4 ◦ C. Lysates from the same bacteria cultured without phages were used as a mock negative control and were generated through a series of freeze/thaw cycles followed by sonication to closely mimic phage-induced lysis. ..

    Cell Culture:

    Article Title: Differential translocation of bacteriophages across the intestinal barrier in health and Crohn's disease.
    Article Snippet: McMurdie and Holmes 83 https://www.bioconductor.org/packages/ release/bioc/html/phyloseq.html Samtools 1.9 Li et al. 84 https://www.htslib.org/ SPAdes 3.14.0 Bankevich et al. 85 https://github.com/ablab/spades vContact2 0.9.19 Jang et al. 86 https://bitbucket.org/MAVERICLab/vcontact2 VIBRANT 1.2.1 Kieft et al. 87 https://github.com/AnantharamanLab/ VIBRANT VirSorter2 2.2.3 Guo et al. 88 https://github.com/jiarong/VirSorter2 Cell Reports 45, 116726, January 27, 2026 19 incubation and lysis of the bacteria by the phages, remaining bacteria and debris were removed by centrifugation for 30 min at 3,000 × g, and the supernatant filtered sequentially through 0.45-μm and 0.2-μm cellulose acetate filters (VWR Avantor). .. Phages were concentrated by tangential flow filtration using a Vivaflow cassette system (Sartorius Stedim Biotech) and stored in a final solution of salt-magnesium (SM) buffer (17 mM MgSO 4 ⋅7H 2 O, 100 mM NaCl, 5 mM Tris-HCl (pH 7.5) at 4 ◦ C. Lysates from the same bacteria cultured without phages were used as a mock negative control and were generated through a series of freeze/thaw cycles followed by sonication to closely mimic phage-induced lysis. ..

    Negative Control:

    Article Title: Differential translocation of bacteriophages across the intestinal barrier in health and Crohn's disease.
    Article Snippet: McMurdie and Holmes 83 https://www.bioconductor.org/packages/ release/bioc/html/phyloseq.html Samtools 1.9 Li et al. 84 https://www.htslib.org/ SPAdes 3.14.0 Bankevich et al. 85 https://github.com/ablab/spades vContact2 0.9.19 Jang et al. 86 https://bitbucket.org/MAVERICLab/vcontact2 VIBRANT 1.2.1 Kieft et al. 87 https://github.com/AnantharamanLab/ VIBRANT VirSorter2 2.2.3 Guo et al. 88 https://github.com/jiarong/VirSorter2 Cell Reports 45, 116726, January 27, 2026 19 incubation and lysis of the bacteria by the phages, remaining bacteria and debris were removed by centrifugation for 30 min at 3,000 × g, and the supernatant filtered sequentially through 0.45-μm and 0.2-μm cellulose acetate filters (VWR Avantor). .. Phages were concentrated by tangential flow filtration using a Vivaflow cassette system (Sartorius Stedim Biotech) and stored in a final solution of salt-magnesium (SM) buffer (17 mM MgSO 4 ⋅7H 2 O, 100 mM NaCl, 5 mM Tris-HCl (pH 7.5) at 4 ◦ C. Lysates from the same bacteria cultured without phages were used as a mock negative control and were generated through a series of freeze/thaw cycles followed by sonication to closely mimic phage-induced lysis. ..

    Generated:

    Article Title: Differential translocation of bacteriophages across the intestinal barrier in health and Crohn's disease.
    Article Snippet: McMurdie and Holmes 83 https://www.bioconductor.org/packages/ release/bioc/html/phyloseq.html Samtools 1.9 Li et al. 84 https://www.htslib.org/ SPAdes 3.14.0 Bankevich et al. 85 https://github.com/ablab/spades vContact2 0.9.19 Jang et al. 86 https://bitbucket.org/MAVERICLab/vcontact2 VIBRANT 1.2.1 Kieft et al. 87 https://github.com/AnantharamanLab/ VIBRANT VirSorter2 2.2.3 Guo et al. 88 https://github.com/jiarong/VirSorter2 Cell Reports 45, 116726, January 27, 2026 19 incubation and lysis of the bacteria by the phages, remaining bacteria and debris were removed by centrifugation for 30 min at 3,000 × g, and the supernatant filtered sequentially through 0.45-μm and 0.2-μm cellulose acetate filters (VWR Avantor). .. Phages were concentrated by tangential flow filtration using a Vivaflow cassette system (Sartorius Stedim Biotech) and stored in a final solution of salt-magnesium (SM) buffer (17 mM MgSO 4 ⋅7H 2 O, 100 mM NaCl, 5 mM Tris-HCl (pH 7.5) at 4 ◦ C. Lysates from the same bacteria cultured without phages were used as a mock negative control and were generated through a series of freeze/thaw cycles followed by sonication to closely mimic phage-induced lysis. ..

    Sonication:

    Article Title: Differential translocation of bacteriophages across the intestinal barrier in health and Crohn's disease.
    Article Snippet: McMurdie and Holmes 83 https://www.bioconductor.org/packages/ release/bioc/html/phyloseq.html Samtools 1.9 Li et al. 84 https://www.htslib.org/ SPAdes 3.14.0 Bankevich et al. 85 https://github.com/ablab/spades vContact2 0.9.19 Jang et al. 86 https://bitbucket.org/MAVERICLab/vcontact2 VIBRANT 1.2.1 Kieft et al. 87 https://github.com/AnantharamanLab/ VIBRANT VirSorter2 2.2.3 Guo et al. 88 https://github.com/jiarong/VirSorter2 Cell Reports 45, 116726, January 27, 2026 19 incubation and lysis of the bacteria by the phages, remaining bacteria and debris were removed by centrifugation for 30 min at 3,000 × g, and the supernatant filtered sequentially through 0.45-μm and 0.2-μm cellulose acetate filters (VWR Avantor). .. Phages were concentrated by tangential flow filtration using a Vivaflow cassette system (Sartorius Stedim Biotech) and stored in a final solution of salt-magnesium (SM) buffer (17 mM MgSO 4 ⋅7H 2 O, 100 mM NaCl, 5 mM Tris-HCl (pH 7.5) at 4 ◦ C. Lysates from the same bacteria cultured without phages were used as a mock negative control and were generated through a series of freeze/thaw cycles followed by sonication to closely mimic phage-induced lysis. ..

    Lysis:

    Article Title: Differential translocation of bacteriophages across the intestinal barrier in health and Crohn's disease.
    Article Snippet: McMurdie and Holmes 83 https://www.bioconductor.org/packages/ release/bioc/html/phyloseq.html Samtools 1.9 Li et al. 84 https://www.htslib.org/ SPAdes 3.14.0 Bankevich et al. 85 https://github.com/ablab/spades vContact2 0.9.19 Jang et al. 86 https://bitbucket.org/MAVERICLab/vcontact2 VIBRANT 1.2.1 Kieft et al. 87 https://github.com/AnantharamanLab/ VIBRANT VirSorter2 2.2.3 Guo et al. 88 https://github.com/jiarong/VirSorter2 Cell Reports 45, 116726, January 27, 2026 19 incubation and lysis of the bacteria by the phages, remaining bacteria and debris were removed by centrifugation for 30 min at 3,000 × g, and the supernatant filtered sequentially through 0.45-μm and 0.2-μm cellulose acetate filters (VWR Avantor). .. Phages were concentrated by tangential flow filtration using a Vivaflow cassette system (Sartorius Stedim Biotech) and stored in a final solution of salt-magnesium (SM) buffer (17 mM MgSO 4 ⋅7H 2 O, 100 mM NaCl, 5 mM Tris-HCl (pH 7.5) at 4 ◦ C. Lysates from the same bacteria cultured without phages were used as a mock negative control and were generated through a series of freeze/thaw cycles followed by sonication to closely mimic phage-induced lysis. ..

    Produced:

    Article Title: Microbiome as a Tool to Monitor Aquarium Systems
    Article Snippet: .. Concentration of Nucleic Acids from Water Samples To optimize the DNA yield, water samples were concentrated using a tangential flow filtration (TFF) system under negative pressure produced by a Masterflex Easy-loadTM pump (Thermo Fisher Scientific Inc., Waltham, MA, USA), and the water was flowed through a VivaflowTM 50 tangential flow cassette (Sartorius AG, Goettingen, Germany). ..



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    Figure Lengend Snippet:

    Article Snippet: Tangential flow filtration 100 kDa hollow fiber membrane , Repligen , Cat# D02-E100-05-N.

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