syntaxin 4 (Santa Cruz Biotechnology)
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Syntaxin 4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/syntaxin/Syntaxin+4+Antibody/pm11208155-200-19-40
Average 92 stars, based on 27 article reviews
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Blocking Assay:Article Title: CRISPR–Cas9 screens reveal regulators of ageing in neural stem cells Article Snippet: Slides were treated with 50–70 μl blocking solution (5% normal donkey serum (NDS, ImmunoReagents, SP-072-VX10), 1% BSA (Sigma-Aldrich, A1595-50ML), 8.5 ml PBS) in a humidified chamber at room temperature for 30 min. .. Blocking solution was replaced with antibody solution consisting of blocking solution with antibodies as follows: mCherry (Invitrogen, M11217, clone 16D7) 1:500, GFAP (Abcam, 53554) 1:500, GFP (Abcam, 13970) 1:500, GLUT4 (for in vivo staining R&D Systems, MAB1262, clone 1F8) 1:500, Ki67 (Invitrogen, 14-5698-082, clone SolA15) 1:500, Article Title: CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells. Article Snippet: Slides were treated with 50–70 μl blocking solution (5% normal donkey serum (NDS, ImmunoReagents, SP-072-VX10), 1% BSA (Sigma-Aldrich, A1595-50ML), 8.5 ml PBS) in a humidified chamber at room temperature for 30 min. .. Blocking solution was replaced with antibody solution consisting of blocking solution with antibodies as follows: mCherry (Invitrogen, M11217, clone 16D7) 1:500, GFAP (Abcam, 53554) 1:500, GFP (Abcam, 13970) 1:500, GLUT4 (for in vivo staining R&D Systems, MAB1262, clone 1F8) 1:500, Ki67 (Invitrogen, 14-5698-082, clone SolA15) 1:500, In Vivo:Article Title: CRISPR–Cas9 screens reveal regulators of ageing in neural stem cells Article Snippet: Slides were treated with 50–70 μl blocking solution (5% normal donkey serum (NDS, ImmunoReagents, SP-072-VX10), 1% BSA (Sigma-Aldrich, A1595-50ML), 8.5 ml PBS) in a humidified chamber at room temperature for 30 min. .. Blocking solution was replaced with antibody solution consisting of blocking solution with antibodies as follows: mCherry (Invitrogen, M11217, clone 16D7) 1:500, GFAP (Abcam, 53554) 1:500, GFP (Abcam, 13970) 1:500, GLUT4 (for in vivo staining R&D Systems, MAB1262, clone 1F8) 1:500, Ki67 (Invitrogen, 14-5698-082, clone SolA15) 1:500, Article Title: CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells. Article Snippet: Slides were treated with 50–70 μl blocking solution (5% normal donkey serum (NDS, ImmunoReagents, SP-072-VX10), 1% BSA (Sigma-Aldrich, A1595-50ML), 8.5 ml PBS) in a humidified chamber at room temperature for 30 min. .. Blocking solution was replaced with antibody solution consisting of blocking solution with antibodies as follows: mCherry (Invitrogen, M11217, clone 16D7) 1:500, GFAP (Abcam, 53554) 1:500, GFP (Abcam, 13970) 1:500, GLUT4 (for in vivo staining R&D Systems, MAB1262, clone 1F8) 1:500, Ki67 (Invitrogen, 14-5698-082, clone SolA15) 1:500, Article Title: CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells. Article Snippet: Alexa 488/594/647 conjugated antibodies, (Fisher Scientific, A21206, A21209, A31571) 1:500. .. Immunofluorescence straining in brain sections in vivo: mCherry (Invitrogen, M11217 clone 16D7) 1:500, GFAP (Abcam, 53554) 1:500, GFP (Abcam, 13970) 1:500, GLUT4 (R&D Systems, MAB1262 clone 1F8) 1:500, Ki67 (Invitrogen, 14-5698-082 clone SolA15) 1:500, Staining:Article Title: CRISPR–Cas9 screens reveal regulators of ageing in neural stem cells Article Snippet: Slides were treated with 50–70 μl blocking solution (5% normal donkey serum (NDS, ImmunoReagents, SP-072-VX10), 1% BSA (Sigma-Aldrich, A1595-50ML), 8.5 ml PBS) in a humidified chamber at room temperature for 30 min. .. Blocking solution was replaced with antibody solution consisting of blocking solution with antibodies as follows: mCherry (Invitrogen, M11217, clone 16D7) 1:500, GFAP (Abcam, 53554) 1:500, GFP (Abcam, 13970) 1:500, GLUT4 (for in vivo staining R&D Systems, MAB1262, clone 1F8) 1:500, Ki67 (Invitrogen, 14-5698-082, clone SolA15) 1:500, Article Title: CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells. Article Snippet: Slides were treated with 50–70 μl blocking solution (5% normal donkey serum (NDS, ImmunoReagents, SP-072-VX10), 1% BSA (Sigma-Aldrich, A1595-50ML), 8.5 ml PBS) in a humidified chamber at room temperature for 30 min. .. Blocking solution was replaced with antibody solution consisting of blocking solution with antibodies as follows: mCherry (Invitrogen, M11217, clone 16D7) 1:500, GFAP (Abcam, 53554) 1:500, GFP (Abcam, 13970) 1:500, GLUT4 (for in vivo staining R&D Systems, MAB1262, clone 1F8) 1:500, Ki67 (Invitrogen, 14-5698-082, clone SolA15) 1:500, other:Article Title: α-Synuclein Deletion Impairs Platelet Function: A Role for SNARE Complex Assembly Article Snippet: FITC-labelled CD41 antibody (553848) was purchased from BD Pharmingen (Franklin Lakes, NJ, USA). α-synuclein (sc-515879), P-α-synuclein (ser129, sc-135638), syntaxin 11 (sc-377121), syntaxin 4 (sc-101301), and Incubation:Article Title: Alpha-Taxilin: A Potential Diagnosis and Therapeutics Target in Rheumatoid Arthritis Which Interacts with Key Glycolytic Enzymes Associated with Metabolic Shifts in Fibroblast-Like Synoviocytes Article Snippet: The lysate was centrifuged (15,000 × g, 4°C, 30 min), and protein 40 microgram (μg) estimated by bicinchoninic acid assay (BCA) (G-biosciences, USA) was run on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a nitrocellulose membrane (NC) (G-biosciences, USA) using a semi dry transfer unit (Bio-Rad, USA). .. The membranes were then incubated (4°C overnight) with 5% Bovine Serum Albumin (BSA), followed by washing and incubation (overnight at 4°C) separately with diluted (1:3000) primary antibodies; Anti-p-65 (Santa Cruz, USA), anti-α-Taxilin (Santa Cruz, USA), anti- glyceraldehyde3-phosphate dehydrogenase (GAPDH) (Santa Cruz, USA), anti-STX-3 (Santa Cruz, USA), Article Title: Alpha-Taxilin: A Potential Diagnosis and Therapeutics Target in Rheumatoid Arthritis Which Interacts with Key Glycolytic Enzymes Associated with Metabolic Shifts in Fibroblast-Like Synoviocytes Article Snippet: The lysate was centrifuged (15,000 × g, 4°C, 30 min), and protein 40 microgram (μg) estimated by bicinchoninic acid assay (BCA) (G-biosciences, USA) was run on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a nitrocellulose membrane (NC) (G-biosciences, USA) using a semi dry transfer unit (Bio-Rad, USA). .. The membranes were then incubated (4°C overnight) with 5% Bovine Serum Albumin (BSA), followed by washing and incubation (overnight at 4°C) separately with diluted (1:3000) primary antibodies; Anti-p-65 (Santa Cruz, USA), anti-α-Taxilin (Santa Cruz, USA), anti- glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Santa Cruz, USA), anti-STX-3 (Santa Cruz, USA), Article Title: Carbon Dot-Loaded Apoptotic Vesicles Improve the Liver Kupffer Cell-Mediated Antibacterial Effect to Synergistically Alleviate Sepsis. Article Snippet: Sepsis is a lethal systemic inflammatory disease against infection that lacks effective therapeutic approaches.. Liver resident macrophage Kupffer cell (KC)-initiated bacterial clearance is crucial for the host to defend against infection.. However, it remains unclear whether this process also governs the antibacterial therapy of sepsis that would be used to improve therapeutic outcomes. Chromatin Immunoprecipitation:Article Title: CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells. Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Immunofluorescence: In vitro: GLUT4 (Abcam, 33780) 1:500, Ki67 (Invitrogen, 14-5698-082 clone SolA15) 1:500, Flow Cytometry:Article Title: CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells. Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Immunofluorescence: In vitro: GLUT4 (Abcam, 33780) 1:500, Ki67 (Invitrogen, 14-5698-082 clone SolA15) 1:500, Magnetic Resonance Imaging:Article Title: CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells. Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Immunofluorescence: In vitro: GLUT4 (Abcam, 33780) 1:500, Ki67 (Invitrogen, 14-5698-082 clone SolA15) 1:500, Immunofluorescence:Article Title: CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells. Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Immunofluorescence: In vitro: GLUT4 (Abcam, 33780) 1:500, Ki67 (Invitrogen, 14-5698-082 clone SolA15) 1:500, Article Title: CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells. Article Snippet: Alexa 488/594/647 conjugated antibodies, (Fisher Scientific, A21206, A21209, A31571) 1:500. .. Immunofluorescence straining in brain sections in vivo: mCherry (Invitrogen, M11217 clone 16D7) 1:500, GFAP (Abcam, 53554) 1:500, GFP (Abcam, 13970) 1:500, GLUT4 (R&D Systems, MAB1262 clone 1F8) 1:500, Ki67 (Invitrogen, 14-5698-082 clone SolA15) 1:500, In Vitro:Article Title: CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells. Article Snippet: .. 3 nature portfolio | reporting sum m ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Immunofluorescence: In vitro: GLUT4 (Abcam, 33780) 1:500, Ki67 (Invitrogen, 14-5698-082 clone SolA15) 1:500, |
