syntaxin 12 13 (Synaptic Systems)
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syntaxin 12 13
Syntaxin 12 13, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/syntaxin+13/110+131/pmc06504893-211-26-28
Average 90 stars, based on 7 article reviews
Syntaxin 12 13, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/syntaxin+13/110+131/pmc06504893-211-26-28
Average 90 stars, based on 7 article reviews
syntaxin 12 13 - by Bioz Stars,
2026-10
90/100 stars
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Western Blot:Article Title: Synaptotagmin 17 controls neurite outgrowth and synaptic physiology via distinct cellular pathways Article Snippet: .. The input lysate and eluates were assayed by Western blot using primary antibodies to syntaxin-5 or Article Title: Enhanced Recruitment of Endosomal Na + /H + Exchanger NHE6 into Dendritic Spines of Hippocampal Pyramidal Neurons during NMDA Receptor-Dependent Long-Term Potentiation Article Snippet: All products used for neuronal organotypic slice and primary cell culture were purchased from Gibco/Invitrogen unless otherwise indicated. .. Protein localization studies using immunofluorescence and immunoblotting were performed using the following commercial antibodies: mouse monoclonal anti-hemagglutinin (HA) antibody (Covance), rabbit polyclonal anti-HA (Abcam), anti-β-tubulin antibody (Sigma-Aldrich), anti-transferrin receptor antibody (Invitrogen), Article Title: A BLOC-1 Mutation Screen Reveals that PLDN Is Mutated in Hermansky-Pudlak Syndrome Type 9 Article Snippet: Antibodies Mouse monoclonal antibodies against the following proteins were acquired as follows: syntaxin-13 (used for immunoblotting; clone 15G12, Abcam, Cambridge MA), Myc-tag (clone 9E10), β-actin (Clone AC-15), α-tubulin (clone B-5-1-2; all from Sigma Aldrich, St. Louis MO), TYRP1 (used for immunofluorescence and internalization assays; clone TA99, ATCC, Masasses VA), tyrosinase (clone T311, Santa Cruz Biotechnology, Santa Cruz, CA), and cappuccino (clone S-5; Santa Cruz Biotechnology). .. Rabbit polyclonal antibodies against the following proteins were acquired as follows: Incubation:Article Title: Synaptotagmin 17 controls neurite outgrowth and synaptic physiology via distinct cellular pathways Article Snippet: .. The input lysate and eluates were assayed by Western blot using primary antibodies to syntaxin-5 or Purification:Article Title: A new probe for super-resolution imaging of membranes elucidates trafficking pathways Article Snippet: .. The following antibodies were used: VGLUT1/2 (rabbit polyclonal, 135 503; Synaptic Systems), synaptophysin (rabbit polyclonal raised against synaptophysin purified from rat synaptic vesicles; see ; provided by R. Jahn), synaptotagmin 1 (rabbit polyclonal, 105 102; Synaptic Systems), VAMP2 (mouse monoclonal, 104211; Synaptic Systems), synapsin (rabbit polyclonal, 106 002; Synaptic Systems), Immunofluorescence:Article Title: Enhanced Recruitment of Endosomal Na + /H + Exchanger NHE6 into Dendritic Spines of Hippocampal Pyramidal Neurons during NMDA Receptor-Dependent Long-Term Potentiation Article Snippet: All products used for neuronal organotypic slice and primary cell culture were purchased from Gibco/Invitrogen unless otherwise indicated. .. Protein localization studies using immunofluorescence and immunoblotting were performed using the following commercial antibodies: mouse monoclonal anti-hemagglutinin (HA) antibody (Covance), rabbit polyclonal anti-HA (Abcam), anti-β-tubulin antibody (Sigma-Aldrich), anti-transferrin receptor antibody (Invitrogen), Article Title: A BLOC-1 Mutation Screen Reveals that PLDN Is Mutated in Hermansky-Pudlak Syndrome Type 9 Article Snippet: Antibodies Mouse monoclonal antibodies against the following proteins were acquired as follows: syntaxin-13 (used for immunoblotting; clone 15G12, Abcam, Cambridge MA), Myc-tag (clone 9E10), β-actin (Clone AC-15), α-tubulin (clone B-5-1-2; all from Sigma Aldrich, St. Louis MO), TYRP1 (used for immunofluorescence and internalization assays; clone TA99, ATCC, Masasses VA), tyrosinase (clone T311, Santa Cruz Biotechnology, Santa Cruz, CA), and cappuccino (clone S-5; Santa Cruz Biotechnology). .. Rabbit polyclonal antibodies against the following proteins were acquired as follows: other:Article Title: Comparative synaptosome imaging: a semi-quantitative method to obtain copy numbers for synaptic and neuronal proteins Article Snippet: Syntaxin13 , 13.925 , 0.842 ( |
Figure 1 from ∼100 organs of Corti (per biological replicate) and used as starting material in VGluT3-and control IgG-specific immunoisolations. Two technical replicates (indicated as T1 and T2) from two independent immunoisolation procedures (biological replicates R1 and R2) were measured. B and C , approach used for the analysis of the MS data. B , protein enrichment was assessed by comparing VGluT3 immunoisolates with both control IgG immunoisolates and input S2 samples. The –log 10 adjusted p value was plotted against the log 2 iBAQ fold change of VGluT3 over control (IgG or input), with a significant t test FDR threshold of 5% and S 0 = 0. C , to visualize protein enrichment in VGluT3 immunoisolates as compared to both control IgG and input S2, log 2 iBAQ fold change VGluT3/Control IgG vs. log 2 iBAQ fold change VGluT3/Input was plotted; proteins in the upper right quadrant were enriched in VGluT3 immunoisolates – a threshold of log 2 iBAQ fold difference >0.7 was set to consider only proteins with at least 1.5-fold enrichment. D and E , Scatter plots showing differential enrichment of proteins in VGluT3 immunoisolates when compared to both control IgG and inputs at P8 ( D ) and P23 ( E ); displayed are IHC marker proteins VGluT3 and otoferlin, classical SV proteins, SNAREs, SNARE-binding proteins and other proteins. Numbers in parenthesis refer to the total number of significantly enriched proteins in VGluT3 immunoisolates over control IgG and Input (>1.5-fold enrichment). Gene names are displayed. Stx12 gene annotated in UniProt refers to 
Figure 4 , D and E ( upper right quadrant and >1.5-fold enrichment). Stx12 gene annotated in UniProt refers to syntaxin-12/13 protein (syntaxin-12 and syntaxin-13 are the same protein; syntaxin-13 is the accepted term by the scientific community, but is still annotated in most databases as syntaxin-12). Source data are available for this figure ( 
supplemental Table S4 . B–F , Scatter plots showing positively enriched proteins in VGluT3 immunoisolates at P23 when compared to both control IgG and input; log 2 iBAQ fold change VGluT3/Control IgG vs. log 2 iBAQ fold change VGluT3/Input was plotted. Displayed are proteins involved in trafficking events in different trafficking organelles (SV, endolysosomal, Golgi, and ER proteins) including SNAREs and resident proteins. Several Rab GTPase proteins, mostly of endolysosomal nature, were also enriched ( F ). Annotations were done manually and based on information available in several databases; proteins were grouped according to cellular compartment and biological function. For detailed annotation see 