anti-syntaxin 13 (Synaptic Systems)
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Anti Syntaxin 13, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/syntaxin+13/syntaxin+13+antibody/pmc05699080-188-34-43
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Purification:Article Title: A new probe for super-resolution imaging of membranes elucidates trafficking pathways. Article Snippet: .. The following antibodies were used: VGLUT1/2 (rabbit polyclonal, 135 503; Synaptic Systems), synaptophysin (rabbit polyclonal raised against synaptophysin purified from rat synaptic vesicles; see Jahn et al., 1985; provided by R. Jahn), synaptotagmin 1 (rabbit polyclonal, 105 102; Synaptic Systems), VAMP2 (mouse monoclonal, 104211; Synaptic Systems), synapsin (rabbit polyclonal, 106 002; Synaptic Systems), Article Title: A new probe for super-resolution imaging of membranes elucidates trafficking pathways Article Snippet: .. The following antibodies were used: VGLUT1/2 (rabbit polyclonal, 135 503; Synaptic Systems), synaptophysin (rabbit polyclonal raised against synaptophysin purified from rat synaptic vesicles; see ; provided by R. Jahn), synaptotagmin 1 (rabbit polyclonal, 105 102; Synaptic Systems), VAMP2 (mouse monoclonal, 104211; Synaptic Systems), synapsin (rabbit polyclonal, 106 002; Synaptic Systems), Incubation:Article Title: Axonal BACE1 dynamics and targeting in hippocampal neurons: a role for Rab11 GTPase Article Snippet: .. The coverslips were incubated for 1 h at room temperature with the primary antibodies diluted in PBS containing 3% BSA: MAP2 mAb (1:5,000; Sigma), EEA1 (1:200; Millipore), other:Article Title: Phosphorylation of Synapsin I by cAMP-Dependent Protein Kinase Controls Synaptic Vesicle Dynamics in Developing Neurons Article Snippet: The anti-syntaxin 13 polyclonal antibody and anti-synaptotagmin I [cytoplasmic domain (SytC) and Article Title: Phosphorylation of Synapsin I by cAMP-Dependent Protein Kinase Controls Synaptic Vesicle Dynamics in Developing Neurons Article Snippet: The anti-syntaxin 13 polyclonal antibody and anti-synaptotagmin I [cytoplasmic domain (Syt C ) and Article Title: Nuclear Calcium/Calmodulin Regulates Memory Consolidation Article Snippet: Monoclonal anti-CaM and polyclonal anti-Fos were obtained from Upstate Biotechnology (Lake Placid, NY); polyclonal anti-, total-, and phospho-MAPK were from Cell Signaling Technology (Beverly, MA); the nuclear loading control monoclonal anti-neuronal nuclei was from Chemicon (Temecula, CA); the cytoplasmic Article Title: Probing and manipulating intracellular membrane traffic by microinjection of artificial vesicles Article Snippet: Primary antibodies were obtained from the following companies: anti-GM130, anti-EEA1, and anti-p150 Glued from BD Biosciences; anti-LAMP1, anti–α-tubulin, and anti-mitofilin from Abcam; anti-FLAG from Sigma-Aldrich; anti-dynein intermediate chain from Millipore; anti-PDI from ABfinity; anti-GFP, anti-syntaxin 6, anti-syntaxin 13, anti-vti1a, anti-VAMP4, and |
Figure 1 from ∼100 organs of Corti (per biological replicate) and used as starting material in VGluT3-and control IgG-specific immunoisolations. Two technical replicates (indicated as T1 and T2) from two independent immunoisolation procedures (biological replicates R1 and R2) were measured. B and C , approach used for the analysis of the MS data. B , protein enrichment was assessed by comparing VGluT3 immunoisolates with both control IgG immunoisolates and input S2 samples. The –log 10 adjusted p value was plotted against the log 2 iBAQ fold change of VGluT3 over control (IgG or input), with a significant t test FDR threshold of 5% and S 0 = 0. C , to visualize protein enrichment in VGluT3 immunoisolates as compared to both control IgG and input S2, log 2 iBAQ fold change VGluT3/Control IgG vs. log 2 iBAQ fold change VGluT3/Input was plotted; proteins in the upper right quadrant were enriched in VGluT3 immunoisolates – a threshold of log 2 iBAQ fold difference >0.7 was set to consider only proteins with at least 1.5-fold enrichment. D and E , Scatter plots showing differential enrichment of proteins in VGluT3 immunoisolates when compared to both control IgG and inputs at P8 ( D ) and P23 ( E ); displayed are IHC marker proteins VGluT3 and otoferlin, classical SV proteins, SNAREs, SNARE-binding proteins and other proteins. Numbers in parenthesis refer to the total number of significantly enriched proteins in VGluT3 immunoisolates over control IgG and Input (>1.5-fold enrichment). Gene names are displayed. Stx12 gene annotated in UniProt refers to 
Figure 4 , D and E ( upper right quadrant and >1.5-fold enrichment). Stx12 gene annotated in UniProt refers to syntaxin-12/13 protein (syntaxin-12 and syntaxin-13 are the same protein; syntaxin-13 is the accepted term by the scientific community, but is still annotated in most databases as syntaxin-12). Source data are available for this figure (
supplemental Table S4 . B–F , Scatter plots showing positively enriched proteins in VGluT3 immunoisolates at P23 when compared to both control IgG and input; log 2 iBAQ fold change VGluT3/Control IgG vs. log 2 iBAQ fold change VGluT3/Input was plotted. Displayed are proteins involved in trafficking events in different trafficking organelles (SV, endolysosomal, Golgi, and ER proteins) including SNAREs and resident proteins. Several Rab GTPase proteins, mostly of endolysosomal nature, were also enriched ( F ). Annotations were done manually and based on information available in several databases; proteins were grouped according to cellular compartment and biological function. For detailed annotation see