anti-syntaxin-12 (Synaptic Systems)
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Anti Syntaxin 12, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/syntaxin+12/anti+syntaxin+12/pmc05577145-256-61-64
Average 90 stars, based on 1 article reviews
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1) Product Images from "The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes"
Article Title: The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes
Journal: Scientific Reports
doi: 10.1038/s41598-017-10607-4
Figure Legend Snippet: Immunohistochemical detection of TPC1 in kidney and co-localization with syntaxin-12. ( a ) TPC1 (red) is predominantly expressed in the proximal tubule as indicated by co-staining with the marker Lotus tetragonolobus lectin (green) (Vector laboratories #B1325). ( b ) Control staining using a kidney section from a TPC1 knock-out mouse. Staining with antibodies as in ( a ), scale bar = 100 µm for ( a ) and ( b ). ( c ) Co-localization of TPC1 (red, anti-TPC1, generated by Gramsch Laboratories) with syntaxin-12 (green, anti-STX12 Synaptic Systems) at the apical membrane of kidney proximal tubules. DAPI staining in blue; scale bar = 20 µm.
Techniques Used: Immunohistochemical staining, Staining, Marker, Plasmid Preparation, Knock-Out, Generated
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Incubation:Article Title: The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes Article Snippet: For antigen retrieval kidney cryosections were treated with citrate buffer (pH 6, microwave heating for 10 min) and then incubated with blocking solution (phosphate buffer containing 1% Triton X-100, 1% bovine serum albumin and 5% normal goat serum) for 1–2 h. Sections were incubated for 3 h at room temperature with either the anti-TPC1 antibody (5 μg/ml in blocking solution) and Article Title: The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes. Article Snippet: For antigen retrieval kidney cryosections were treated with citrate buffer (pH 6, microwave heating for 10 min) and then incubated with blocking solution (phosphate buffer containing 1% Triton X-100, 1% bovine serum albumin and 5% normal goat serum) for 1–2 h. Sections were incubated for 3 h at room temperature with either the anti-TPC1 antibody (5 μg/ml in blocking solution) and Blocking Assay:Article Title: The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes Article Snippet: For antigen retrieval kidney cryosections were treated with citrate buffer (pH 6, microwave heating for 10 min) and then incubated with blocking solution (phosphate buffer containing 1% Triton X-100, 1% bovine serum albumin and 5% normal goat serum) for 1–2 h. Sections were incubated for 3 h at room temperature with either the anti-TPC1 antibody (5 μg/ml in blocking solution) and Article Title: The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes. Article Snippet: For antigen retrieval kidney cryosections were treated with citrate buffer (pH 6, microwave heating for 10 min) and then incubated with blocking solution (phosphate buffer containing 1% Triton X-100, 1% bovine serum albumin and 5% normal goat serum) for 1–2 h. Sections were incubated for 3 h at room temperature with either the anti-TPC1 antibody (5 μg/ml in blocking solution) and Immunohistochemical staining:Article Title: The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes Article Snippet: For antigen retrieval kidney cryosections were treated with citrate buffer (pH 6, microwave heating for 10 min) and then incubated with blocking solution (phosphate buffer containing 1% Triton X-100, 1% bovine serum albumin and 5% normal goat serum) for 1–2 h. Sections were incubated for 3 h at room temperature with either the anti-TPC1 antibody (5 μg/ml in blocking solution) and Article Title: The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes. Article Snippet: For antigen retrieval kidney cryosections were treated with citrate buffer (pH 6, microwave heating for 10 min) and then incubated with blocking solution (phosphate buffer containing 1% Triton X-100, 1% bovine serum albumin and 5% normal goat serum) for 1–2 h. Sections were incubated for 3 h at room temperature with either the anti-TPC1 antibody (5 μg/ml in blocking solution) and Staining:Article Title: The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes Article Snippet: For antigen retrieval kidney cryosections were treated with citrate buffer (pH 6, microwave heating for 10 min) and then incubated with blocking solution (phosphate buffer containing 1% Triton X-100, 1% bovine serum albumin and 5% normal goat serum) for 1–2 h. Sections were incubated for 3 h at room temperature with either the anti-TPC1 antibody (5 μg/ml in blocking solution) and Article Title: The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes. Article Snippet: For antigen retrieval kidney cryosections were treated with citrate buffer (pH 6, microwave heating for 10 min) and then incubated with blocking solution (phosphate buffer containing 1% Triton X-100, 1% bovine serum albumin and 5% normal goat serum) for 1–2 h. Sections were incubated for 3 h at room temperature with either the anti-TPC1 antibody (5 μg/ml in blocking solution) and Marker:Article Title: The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes Article Snippet: For antigen retrieval kidney cryosections were treated with citrate buffer (pH 6, microwave heating for 10 min) and then incubated with blocking solution (phosphate buffer containing 1% Triton X-100, 1% bovine serum albumin and 5% normal goat serum) for 1–2 h. Sections were incubated for 3 h at room temperature with either the anti-TPC1 antibody (5 μg/ml in blocking solution) and Article Title: The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes. Article Snippet: For antigen retrieval kidney cryosections were treated with citrate buffer (pH 6, microwave heating for 10 min) and then incubated with blocking solution (phosphate buffer containing 1% Triton X-100, 1% bovine serum albumin and 5% normal goat serum) for 1–2 h. Sections were incubated for 3 h at room temperature with either the anti-TPC1 antibody (5 μg/ml in blocking solution) and Plasmid Preparation:Article Title: The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes Article Snippet: For antigen retrieval kidney cryosections were treated with citrate buffer (pH 6, microwave heating for 10 min) and then incubated with blocking solution (phosphate buffer containing 1% Triton X-100, 1% bovine serum albumin and 5% normal goat serum) for 1–2 h. Sections were incubated for 3 h at room temperature with either the anti-TPC1 antibody (5 μg/ml in blocking solution) and Article Title: The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes. Article Snippet: For antigen retrieval kidney cryosections were treated with citrate buffer (pH 6, microwave heating for 10 min) and then incubated with blocking solution (phosphate buffer containing 1% Triton X-100, 1% bovine serum albumin and 5% normal goat serum) for 1–2 h. Sections were incubated for 3 h at room temperature with either the anti-TPC1 antibody (5 μg/ml in blocking solution) and Knock-Out:Article Title: The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes Article Snippet: For antigen retrieval kidney cryosections were treated with citrate buffer (pH 6, microwave heating for 10 min) and then incubated with blocking solution (phosphate buffer containing 1% Triton X-100, 1% bovine serum albumin and 5% normal goat serum) for 1–2 h. Sections were incubated for 3 h at room temperature with either the anti-TPC1 antibody (5 μg/ml in blocking solution) and Article Title: The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes. Article Snippet: For antigen retrieval kidney cryosections were treated with citrate buffer (pH 6, microwave heating for 10 min) and then incubated with blocking solution (phosphate buffer containing 1% Triton X-100, 1% bovine serum albumin and 5% normal goat serum) for 1–2 h. Sections were incubated for 3 h at room temperature with either the anti-TPC1 antibody (5 μg/ml in blocking solution) and Generated:Article Title: The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes Article Snippet: For antigen retrieval kidney cryosections were treated with citrate buffer (pH 6, microwave heating for 10 min) and then incubated with blocking solution (phosphate buffer containing 1% Triton X-100, 1% bovine serum albumin and 5% normal goat serum) for 1–2 h. Sections were incubated for 3 h at room temperature with either the anti-TPC1 antibody (5 μg/ml in blocking solution) and Article Title: The two-pore channel TPC1 is required for efficient protein processing through early and recycling endosomes. Article Snippet: For antigen retrieval kidney cryosections were treated with citrate buffer (pH 6, microwave heating for 10 min) and then incubated with blocking solution (phosphate buffer containing 1% Triton X-100, 1% bovine serum albumin and 5% normal goat serum) for 1–2 h. Sections were incubated for 3 h at room temperature with either the anti-TPC1 antibody (5 μg/ml in blocking solution) and |


