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lightcycler 480 sw 1 5 1 program  (Roche)


Bioz Manufacturer Symbol Roche manufactures this product  
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    Structured Review

    Roche lightcycler 480 sw 1 5 1 program
    Lightcycler 480 Sw 1 5 1 Program, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sw+program/LightCycler+480+System/pm40304898-74-20-25
    Average 99 stars, based on 6 article reviews
    lightcycler 480 sw 1 5 1 program - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: Integrated network toxicology and molecular docking revealed that 2-ethylhexyldiphenylphosphoric acid induces nephrotoxicity in chickens by activating the ROS/JNK pathway
    Article Snippet: Subsequently, reverse transcription was performed with a TransGen Biotech kit (Beijing, China) to generate cDNA. .. Quantitative RT-PCR was conducted on a Roche LightCycler® system utilizing the TB Green® Premix Ex TaqTM II system (Roche Corporation, Tokyo, Japan). ..

    Article Title: Valorization of xylose mother liquor into salidroside by engineered Kluyveromyces marxianus
    Article Snippet: Total RNA was extracted using the Spin Column Yeast Total RNA Purification Kit (Sangon Biotech, Shanghai), and first-strand cDNA was synthesized using ToloScript All-in-one RT EasyMix for qPCR (Tolo Biotech, Shanghai) following the manufacturers’ instructions. .. RT-qPCR was performed with 2 × Q3 SYBR qPCR Master Mix (Tolo Biotech, Shanghai) on a LightCycler 96 Real-Time PCR System (Roche, Switzerland) using gene-specific primers ( ). ..

    Article Title: Distinct infectivity, pathogenicity, and adaptive evolution of clade 2.3.4.4 H5N2 avian influenza viruses in chickens in Taiwan
    Article Snippet: .. Reactions targeting the matrix protein (M) gene were performed using the SuperScript® III Platinum® One-Step Quantitative RT-PCR System (Thermo Fisher Scientific, Waltham, Massachusetts, USA) on a LightCycler 480 Instrument II (Roche, Basel, Switzerland). ..

    Article Title: Cadmium-induced apoptosis via ROS/JNK pathway in chicken primary kidney cells and antagonism of taxifolin
    Article Snippet: Total RNA was isolated from chicken kidneys using the TRIzol extraction method, and first-strand cDNA was synthesized using a qRT-PCR synthesis kit (A5001, Promega, Beijing, China) according to the manufacturer's instructions ( ). .. Gene expression levels were detected by qRT-PCR using a Light Cycler® 480 System (Roche, Basel, Switzerland) with fast Universal SYBR Green Master (Roche, Basel, Switzerland). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Sexing chicken embryos by real-time PCR using primers for the KCMF gene located on the W chromosome
    Article Snippet: .. Real-time PCR was performed in 384-well plates using 2XMaster Mix Takyon and LightCycler® 480 instrument II (Roche, Basel, Switzerland). .. Each 10 μL reaction volume contained 2.5 μL of sample, 2.25 μL nuclease-free water, 0.125 μL of each forward and reverse KCMF1 primers (initial concentration: 10 μM) and 5 μL 2X Master Mix Takyon (Eurogentec, UF-NSMT-B0701) containing SyberGreen 1 as the fluorescent probe.

    Article Title: Valorization of xylose mother liquor into salidroside by engineered Kluyveromyces marxianus
    Article Snippet: Total RNA was extracted using the Spin Column Yeast Total RNA Purification Kit (Sangon Biotech, Shanghai), and first-strand cDNA was synthesized using ToloScript All-in-one RT EasyMix for qPCR (Tolo Biotech, Shanghai) following the manufacturers’ instructions. .. RT-qPCR was performed with 2 × Q3 SYBR qPCR Master Mix (Tolo Biotech, Shanghai) on a LightCycler 96 Real-Time PCR System (Roche, Switzerland) using gene-specific primers ( ). ..

    other:

    Article Title: Targeted intestinal delivery of luteolin microcapsules as a precision nutritional strategy to alleviate heat stress and enhance growth performance in broilers
    Article Snippet: RNA concentration was measured using a NanoDrop2000 spectrophotometer. cDNA was synthesized by reverse transcription. qRT-PCR was conducted on a LightCycler 96 System (Roche, Switzerland).

    Gene Expression:

    Article Title: Cadmium-induced apoptosis via ROS/JNK pathway in chicken primary kidney cells and antagonism of taxifolin
    Article Snippet: Total RNA was isolated from chicken kidneys using the TRIzol extraction method, and first-strand cDNA was synthesized using a qRT-PCR synthesis kit (A5001, Promega, Beijing, China) according to the manufacturer's instructions ( ). .. Gene expression levels were detected by qRT-PCR using a Light Cycler® 480 System (Roche, Basel, Switzerland) with fast Universal SYBR Green Master (Roche, Basel, Switzerland). ..

    SYBR Green Assay:

    Article Title: Cadmium-induced apoptosis via ROS/JNK pathway in chicken primary kidney cells and antagonism of taxifolin
    Article Snippet: Total RNA was isolated from chicken kidneys using the TRIzol extraction method, and first-strand cDNA was synthesized using a qRT-PCR synthesis kit (A5001, Promega, Beijing, China) according to the manufacturer's instructions ( ). .. Gene expression levels were detected by qRT-PCR using a Light Cycler® 480 System (Roche, Basel, Switzerland) with fast Universal SYBR Green Master (Roche, Basel, Switzerland). ..



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