svec (OriGene)
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OriGene
svec
Svec, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/svec/Scrambled+shRNA+control+in+pRFP-C-RS+shRNA+Vector/pmc12080998-150-18-13
Average 93 stars, based on 94 article reviews
Svec, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/svec/Scrambled+shRNA+control+in+pRFP-C-RS+shRNA+Vector/pmc12080998-150-18-13
Average 93 stars, based on 94 article reviews
svec - by Bioz Stars,
2026-09
93/100 stars
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shRNA:Article Title: Occludin Is Essential to Maintain Normal Alveolar Barrier Integrity and Its Protective Role During ARDS Progression Article Snippet: The plasmid pcDNA3.1 was from Invitrogen/Thermofisher (Carlsbad, CA, USA, catalog #V79020). pCMV-occludin expresses a GFP-tagged mouse occludin from the CMV promoter (Origene, Rockville, MD, USA, catalog # MG226013). pEGFP-C1 expresses GFP from the CMV promoter (Clontech, Mountain View, CA, USA, catalog # 6084-1). .. Four distinct RFP-expressing Article Title: Clathrin mediates membrane fission and budding by constricting membrane pores. Article Snippet: For knockdown of endogenous CHC in bovine chromaffin cells, three siRNA duplexes for bovine CHC (5′- CCAGAUUGUCGAUGUGUUU-3′, 5′-GCAUCUACAUGAAUAGAAU-3′, and 5′-CUAUGACAGUCGUGUUGUU-3′) labeled with Cyanine 3 and scrambled control siRNA (SIC003) were purchased from Sigma-Aldrich. .. A mixture of these three siRNAs was used for experiments. shRNA plasmids for bovine CHC (5′- CGGTTGCTCTTGTTACGGATAATGCAGTT-3′) and scrambled control (TR30015) tagged with RFP were purchased from Origene. .. The C1573S CHC mutant was generated from pEGFP-C1-CHC17(Addgene# 59799) plasmid. Article Title: Depleting trafficking regulator CASK promotes intercalated disc organization and ventricular function Article Snippet: .. shRNA sequences for CASK 5’-tggagaatgtgaccagagttcgcctggta-3’ (shCASK) and Article Title: Rudhira-mediated microtubule stability controls TGFβ signaling during mouse vascular development Article Snippet: .. In brief, rudhira shRNA vectors (715, 716) and scrambled (NS) control vector (TR30015) (Origene, USA) were microporated into Article Title: A PACAP-activated network for secretion requires coordination of Ca<sup>2+</sup> influx and Ca<sup>2+</sup> mobilization Article Snippet: .. The scrambled shRNA and IP3R1 shRNA plasmids were purchased from OriGene; the 29mer sequence of scrambled shRNA (Catalogue# TR30015) is Article Title: Regulation of cannabinoid and opioid receptor levels by endogenous and pharmacological chaperones . Article Snippet: 1 Regulation of cannabinoid and opioid receptor levels by endogenous and pharmacological chaperones Achla Gupta1, Ivone Gomes1, Aya Osman1, Wakako Fujita2, and Lakshmi A. Devi1@ @Corresponding author: lakshmi.devi@mssm.edu 1Department of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.. 2Current address: Faculty of Pharmacy, Juntendo University, Urayasu, Chiba, Japan Footnote: Funding was from the National Institute of Drug Abuse [DA008863, DA058681] and National Center for Advancing Translational Sciences [R03 TR002940] grants (to LAD), NARSAD Young Investigator award Grant #31297 (to AO), and JSPS KAKENHI [21K07275] grant (to WF).. Conflict-of-interest statement: No author has an actual or perceived conflict of interest with the contents of this article at A SPE T Journals on A ugust 21, 2024 jpet.aspetjournals.org D ow nloaded from 2 Running Title: Regulation of expression of CB1R-DOR @ Corresponding Author: lakshmi.devi@mssm.edu No. of text pages: 33 Article Title: Role of the Two-Pore-Domain Potassium channel TREK-1 (K 2P 2.1) in Hyperoxia- and Mechanical Stretch-Induced Alveolar Epithelial Injury Article Snippet: .. Briefly, for stable TREK-1 knockdown, human AT2 (A549) cells were transfected with anti-TREK-1 shRNA using a commercially available pRFP-C-RS vector (Origene, catalog #TR30015) using the Plasmid Preparation:Article Title: Occludin Is Essential to Maintain Normal Alveolar Barrier Integrity and Its Protective Role During ARDS Progression Article Snippet: The plasmid pcDNA3.1 was from Invitrogen/Thermofisher (Carlsbad, CA, USA, catalog #V79020). pCMV-occludin expresses a GFP-tagged mouse occludin from the CMV promoter (Origene, Rockville, MD, USA, catalog # MG226013). pEGFP-C1 expresses GFP from the CMV promoter (Clontech, Mountain View, CA, USA, catalog # 6084-1). .. Four distinct RFP-expressing Article Title: Depleting trafficking regulator CASK promotes intercalated disc organization and ventricular function Article Snippet: .. shRNA sequences for CASK 5’-tggagaatgtgaccagagttcgcctggta-3’ (shCASK) and Control:Article Title: Clathrin mediates membrane fission and budding by constricting membrane pores. Article Snippet: For knockdown of endogenous CHC in bovine chromaffin cells, three siRNA duplexes for bovine CHC (5′- CCAGAUUGUCGAUGUGUUU-3′, 5′-GCAUCUACAUGAAUAGAAU-3′, and 5′-CUAUGACAGUCGUGUUGUU-3′) labeled with Cyanine 3 and scrambled control siRNA (SIC003) were purchased from Sigma-Aldrich. .. A mixture of these three siRNAs was used for experiments. shRNA plasmids for bovine CHC (5′- CGGTTGCTCTTGTTACGGATAATGCAGTT-3′) and scrambled control (TR30015) tagged with RFP were purchased from Origene. .. The C1573S CHC mutant was generated from pEGFP-C1-CHC17(Addgene# 59799) plasmid. Article Title: Depleting trafficking regulator CASK promotes intercalated disc organization and ventricular function Article Snippet: .. shRNA sequences for CASK 5’-tggagaatgtgaccagagttcgcctggta-3’ (shCASK) and Article Title: Rudhira-mediated microtubule stability controls TGFβ signaling during mouse vascular development Article Snippet: .. In brief, rudhira shRNA vectors (715, 716) and scrambled (NS) control vector (TR30015) (Origene, USA) were microporated into Article Title: Regulation of cannabinoid and opioid receptor levels by endogenous and pharmacological chaperones . Article Snippet: 1 Regulation of cannabinoid and opioid receptor levels by endogenous and pharmacological chaperones Achla Gupta1, Ivone Gomes1, Aya Osman1, Wakako Fujita2, and Lakshmi A. Devi1@ @Corresponding author: lakshmi.devi@mssm.edu 1Department of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.. 2Current address: Faculty of Pharmacy, Juntendo University, Urayasu, Chiba, Japan Footnote: Funding was from the National Institute of Drug Abuse [DA008863, DA058681] and National Center for Advancing Translational Sciences [R03 TR002940] grants (to LAD), NARSAD Young Investigator award Grant #31297 (to AO), and JSPS KAKENHI [21K07275] grant (to WF).. Conflict-of-interest statement: No author has an actual or perceived conflict of interest with the contents of this article at A SPE T Journals on A ugust 21, 2024 jpet.aspetjournals.org D ow nloaded from 2 Running Title: Regulation of expression of CB1R-DOR @ Corresponding Author: lakshmi.devi@mssm.edu No. of text pages: 33 Produced:Article Title: Depleting trafficking regulator CASK promotes intercalated disc organization and ventricular function Article Snippet: .. shRNA sequences for CASK 5’-tggagaatgtgaccagagttcgcctggta-3’ (shCASK) and Negative Control:Article Title: Tenascin-C expression in relation to tumor-stroma interaction in ameloblastoma. Article Snippet: RNA interference Protein knockdown was carried out using small interfering RNA (siRNA) oligonucleotides, si TNC (siRNA for TNC) was purchased from OriGene Technologies (Rockville, MD, USA). .. For negative control, Transfection:Article Title: Tenascin-C expression in relation to tumor-stroma interaction in ameloblastoma. Article Snippet: RNA interference Protein knockdown was carried out using small interfering RNA (siRNA) oligonucleotides, si TNC (siRNA for TNC) was purchased from OriGene Technologies (Rockville, MD, USA). .. For negative control, Article Title: A PACAP-activated network for secretion requires coordination of Ca<sup>2+</sup> influx and Ca<sup>2+</sup> mobilization Article Snippet: .. The scrambled shRNA and IP3R1 shRNA plasmids were purchased from OriGene; the 29mer sequence of scrambled shRNA (Catalogue# TR30015) is Article Title: Role of the Two-Pore-Domain Potassium channel TREK-1 (K 2P 2.1) in Hyperoxia- and Mechanical Stretch-Induced Alveolar Epithelial Injury Article Snippet: .. Briefly, for stable TREK-1 knockdown, human AT2 (A549) cells were transfected with anti-TREK-1 shRNA using a commercially available pRFP-C-RS vector (Origene, catalog #TR30015) using the Sequencing:Article Title: A PACAP-activated network for secretion requires coordination of Ca<sup>2+</sup> influx and Ca<sup>2+</sup> mobilization Article Snippet: .. The scrambled shRNA and IP3R1 shRNA plasmids were purchased from OriGene; the 29mer sequence of scrambled shRNA (Catalogue# TR30015) is Fluorescence:Article Title: A PACAP-activated network for secretion requires coordination of Ca<sup>2+</sup> influx and Ca<sup>2+</sup> mobilization Article Snippet: .. The scrambled shRNA and IP3R1 shRNA plasmids were purchased from OriGene; the 29mer sequence of scrambled shRNA (Catalogue# TR30015) is Knockdown:Article Title: Role of the Two-Pore-Domain Potassium channel TREK-1 (K 2P 2.1) in Hyperoxia- and Mechanical Stretch-Induced Alveolar Epithelial Injury Article Snippet: .. Briefly, for stable TREK-1 knockdown, human AT2 (A549) cells were transfected with anti-TREK-1 shRNA using a commercially available pRFP-C-RS vector (Origene, catalog #TR30015) using the |