other:
Light Microscopy:Article Title: Cancer-associated gene analysis of cervical cytology samples and liquid-based cytology significantly improve endometrial cancer diagnosis sensitivity
Article Snippet: .. They were processed using SurePath kits (Becton-Dickinson and Company) according to manufacture's protocol, and cytologically diagnosed using light microscopy after Papanicolaou staining (Leica Autostainer XL; Leica Biosystems Nussloch GmbH). .. Briefly, samples underwent fixation with 100% ethanol for 10 min, 70% ethanol for 40 sec, 50% ethanol for 60 sec, rinse in tap water, Gill hematoxylin for 100 sec, rinse in tap water, 70% ethanol with 0.5% hydrochloric acid for 30 sec, rinse in tap water, 70% ethanol for 50 sec, 95% ethanol for 60 sec, OG-6 for 3 min, 95% ethanol for 60 sec twice, EA-50 for 4 min, 95% ethanol for 55 sec, 100% ethanol for 30 sec ×3 times then 60 sec, Hemo-De (a xylene substitute for low-toxicity, Falma Inc.) for 5 min ×4 times, all processes were performed at room temperature.
Staining:Article Title: Cancer-associated gene analysis of cervical cytology samples and liquid-based cytology significantly improve endometrial cancer diagnosis sensitivity
Article Snippet: .. They were processed using SurePath kits (Becton-Dickinson and Company) according to manufacture's protocol, and cytologically diagnosed using light microscopy after Papanicolaou staining (Leica Autostainer XL; Leica Biosystems Nussloch GmbH). .. Briefly, samples underwent fixation with 100% ethanol for 10 min, 70% ethanol for 40 sec, 50% ethanol for 60 sec, rinse in tap water, Gill hematoxylin for 100 sec, rinse in tap water, 70% ethanol with 0.5% hydrochloric acid for 30 sec, rinse in tap water, 70% ethanol for 50 sec, 95% ethanol for 60 sec, OG-6 for 3 min, 95% ethanol for 60 sec twice, EA-50 for 4 min, 95% ethanol for 55 sec, 100% ethanol for 30 sec ×3 times then 60 sec, Hemo-De (a xylene substitute for low-toxicity, Falma Inc.) for 5 min ×4 times, all processes were performed at room temperature.
Article Title: Quantifying Podocytes and Parietal Epithelial Cells in Human Urine Using Liquid-based Cytology and WT1 Immunoenzyme Staining
Article Snippet: .. Saline-soaked gauze balls Medicine spoon Conical centrifuge tube (Corning, Falcon ® , catalog number: 352097) or equivalent Kimwipes (KCWW, Kimberly-Clark, catalog number: 34133) or equivalent PAP pen (Abcam, catalog number: ab2601) or equivalent BD Totalys TM Slide Prep Slide Rack (BD, catalog number: 491294) Coverslip (Matsunami Glass, catalog number: C024321) or equivalent BD SurePath TM Manual Method Kit (including settling chamber and positively charged slide) (BD, catalog number: 491266) BD CytoRich TM Red Preservative Fluid (BD, catalog number: 491336) Ethanol (Wako Pure Chemical Industries, catalog number: 059-06957) or equivalent Methanol (Wako Pure Chemical Industries, catalog number: 136-09475) or equivalent Hydrogen peroxide (Wako Pure Chemical Industries, catalog number: 086-07445) or equivalent Phosphate-buffered saline (PBS) (Nichirei Biosciences, catalog number: 415223) or equivalent WT1 antibody (clone 6F-H2) (Agilent Technologies, catalog number: M3561) Antibody diluent (Agilent Technologies, catalog number: S3022) or equivalent N-Histofine ® Simple Stain TM MAX PO (MULTI) (Nichirei Biosciences, catalog number: 414151F) N-Histofine ® DAB-3S Kit (Nichirei Biosciences, catalog number: 415192F) Mayer’s hematoxylin (Muto Pure Chemicals, catalog number: 30002) or equivalent Xylene (Wako Pure Chemical Industries, catalog number: 245-00717) or equivalent Malinol (mounting medium) (Muto Pure Chemicals, catalog number: 20093) or equivalent .. Pipette (Nichiryo, model: Nichipet EXII) or equivalent Centrifuge (Kubota, model: M4000) or equivalent Vortex mixer (Scientific Industries, model: Vortex-Genie 2) or equivalent Microscope (Olympus, model: BX43) or equivalent
Saline:Article Title: Quantifying Podocytes and Parietal Epithelial Cells in Human Urine Using Liquid-based Cytology and WT1 Immunoenzyme Staining
Article Snippet: .. Saline-soaked gauze balls Medicine spoon Conical centrifuge tube (Corning, Falcon ® , catalog number: 352097) or equivalent Kimwipes (KCWW, Kimberly-Clark, catalog number: 34133) or equivalent PAP pen (Abcam, catalog number: ab2601) or equivalent BD Totalys TM Slide Prep Slide Rack (BD, catalog number: 491294) Coverslip (Matsunami Glass, catalog number: C024321) or equivalent BD SurePath TM Manual Method Kit (including settling chamber and positively charged slide) (BD, catalog number: 491266) BD CytoRich TM Red Preservative Fluid (BD, catalog number: 491336) Ethanol (Wako Pure Chemical Industries, catalog number: 059-06957) or equivalent Methanol (Wako Pure Chemical Industries, catalog number: 136-09475) or equivalent Hydrogen peroxide (Wako Pure Chemical Industries, catalog number: 086-07445) or equivalent Phosphate-buffered saline (PBS) (Nichirei Biosciences, catalog number: 415223) or equivalent WT1 antibody (clone 6F-H2) (Agilent Technologies, catalog number: M3561) Antibody diluent (Agilent Technologies, catalog number: S3022) or equivalent N-Histofine ® Simple Stain TM MAX PO (MULTI) (Nichirei Biosciences, catalog number: 414151F) N-Histofine ® DAB-3S Kit (Nichirei Biosciences, catalog number: 415192F) Mayer’s hematoxylin (Muto Pure Chemicals, catalog number: 30002) or equivalent Xylene (Wako Pure Chemical Industries, catalog number: 245-00717) or equivalent Malinol (mounting medium) (Muto Pure Chemicals, catalog number: 20093) or equivalent .. Pipette (Nichiryo, model: Nichipet EXII) or equivalent Centrifuge (Kubota, model: M4000) or equivalent Vortex mixer (Scientific Industries, model: Vortex-Genie 2) or equivalent Microscope (Olympus, model: BX43) or equivalent
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