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superscript iii one-step rt pcr platinum taq hifi kit  (Thermo Fisher)


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    Thermo Fisher superscript iii one-step rt pcr platinum taq hifi kit
    Superscript Iii One Step Rt Pcr Platinum Taq Hifi Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+rt/superscript+iii+one+step+rt+pcr+platinum+taq+hifi/pmc12276314-276-15-27
    Average 90 stars, based on 1 article reviews
    superscript iii one-step rt pcr platinum taq hifi kit - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Enterovirus D68 circulation between 2014 and 2022 in Slovenian children
    Article Snippet: A 575 bp VP1 gene region of EV-D68qRT-pcr positive samples was amplified by using SuperScript® III One-Step RT-PCR platinum® Taq HiFi kit (Invitrogen by Thermo Fisher Scientific, Carlsbad, USA), with in-house developed primers and PCR conditions shown in Table 1.

    Article Title: Screening a living biobank identifies cabazitaxel as a strategy to combat acquired taxol resistance in high-grade serous ovarian cancer
    Article Snippet: Library preparation was performed using the TruSeq DNA PCR-Free Kit (Illumina).

    Amplification:

    Article Title: Mass vaccination with reassortment-impaired live H9N2 avian influenza vaccine
    Article Snippet: Viral RNA extraction of both MLVs (E1 and E5) was carried out using the QIAquick® Viral RNA Mini Kit (Qiagen, Germantown, MD, USA). .. Viral RNA was amplified by RT-PCR using SuperScript III One-Step RT-PCR Platinum Taq HiFi DNA polymerase (ThermoFisher). ..

    Article Title: Patterns of HIV-1 Drug Resistance Observed Through Geospatial Analysis of Routine Diagnostic Testing in KwaZulu-Natal, South Africa
    Article Snippet: We performed reverse transcription and nested polymerase chain reaction (PCR) amplification of the protease (PR) and reverse transcriptase (RT) genes using the Applied Biosystems HIV-1 Genotyping Kit (ThermoFisher Scientific, Waltham, MA, USA). .. We reversed transcribed and amplified the integrase (IN) gene using Superscript III one-step RT-PCR system with HiFi Platinum Taq (ThermoFisher Scientific, Waltham, MA, USA) and conducted nested PCR using the Expand High Fidelity Plus PCR System (Merck Lifesciences, Rockville, MD, USA). ..

    Article Title: Attenuation of Chikungunya Virus by a Single Amino Acid Substitution in the nsP1 Component of a Non-Structural Polyprotein.
    Article Snippet: .. The genome sequence was determined using an Applied Biosystems 3730 DNA analyser from overlapping cDNA fragments amplified by RT-PCR using a Superscript III One-step RT-PCR Platinum® Taq HiFi kit (ThermoFisher Scientific, Dartford, UK). .. Manipulation and analysis of the nucleotide sequence data obtained were undertaken using the Lasergene suite of programs (DNAStar, version 17.2).

    Article Title: A viral protein targets mitochondria and chloroplasts by subverting general import pathways and specific receptors.
    Article Snippet: .. The corresponding cDNAs were RT-PCR amplified using SuperScript III One-Step RT-PCR Platinum Taq HiFi following the supplier protocol (Thermo Fisher Scientific, Carlsbad, CA, USA), and N. benthamiana RNAs. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Mass vaccination with reassortment-impaired live H9N2 avian influenza vaccine
    Article Snippet: Viral RNA extraction of both MLVs (E1 and E5) was carried out using the QIAquick® Viral RNA Mini Kit (Qiagen, Germantown, MD, USA). .. Viral RNA was amplified by RT-PCR using SuperScript III One-Step RT-PCR Platinum Taq HiFi DNA polymerase (ThermoFisher). ..

    Article Title: Attenuation of Chikungunya Virus by a Single Amino Acid Substitution in the nsP1 Component of a Non-Structural Polyprotein.
    Article Snippet: .. The genome sequence was determined using an Applied Biosystems 3730 DNA analyser from overlapping cDNA fragments amplified by RT-PCR using a Superscript III One-step RT-PCR Platinum® Taq HiFi kit (ThermoFisher Scientific, Dartford, UK). .. Manipulation and analysis of the nucleotide sequence data obtained were undertaken using the Lasergene suite of programs (DNAStar, version 17.2).

    Article Title: A viral protein targets mitochondria and chloroplasts by subverting general import pathways and specific receptors.
    Article Snippet: .. The corresponding cDNAs were RT-PCR amplified using SuperScript III One-Step RT-PCR Platinum Taq HiFi following the supplier protocol (Thermo Fisher Scientific, Carlsbad, CA, USA), and N. benthamiana RNAs. ..

    One Step RT-PCR:

    Article Title: Mass vaccination with reassortment-impaired live H9N2 avian influenza vaccine
    Article Snippet: Viral RNA extraction of both MLVs (E1 and E5) was carried out using the QIAquick® Viral RNA Mini Kit (Qiagen, Germantown, MD, USA). .. Viral RNA was amplified by RT-PCR using SuperScript III One-Step RT-PCR Platinum Taq HiFi DNA polymerase (ThermoFisher). ..

    Article Title: Influenza A virus resistance to 4'-fluorouridine coincides with viral attenuation in vitro and in vivo.
    Article Snippet: .. One-step RT-PCR was performed using the Superscript III One-Step RT-PCR System with HiFi Platinum Taq DNA polymerase (Thermo #12574035). .. After amplification, samples were cleaned with 0.8×AMPure XP Beads (Beckman Coulter #A63882) and products fractionated on a 1.2% Lonza FlashGel to visualize sizes.

    Article Title: Patterns of HIV-1 Drug Resistance Observed Through Geospatial Analysis of Routine Diagnostic Testing in KwaZulu-Natal, South Africa
    Article Snippet: We performed reverse transcription and nested polymerase chain reaction (PCR) amplification of the protease (PR) and reverse transcriptase (RT) genes using the Applied Biosystems HIV-1 Genotyping Kit (ThermoFisher Scientific, Waltham, MA, USA). .. We reversed transcribed and amplified the integrase (IN) gene using Superscript III one-step RT-PCR system with HiFi Platinum Taq (ThermoFisher Scientific, Waltham, MA, USA) and conducted nested PCR using the Expand High Fidelity Plus PCR System (Merck Lifesciences, Rockville, MD, USA). ..

    Article Title: Unique molecular signatures in rebound viruses from antiretroviral drug and CRISPR-treated HIV-1-infected humanized mice
    Article Snippet: RNA extraction was performed on 50 μL of plasma collected in EDTA blood collection tubes using a Qiagen MinElute virus spin kit (Cat. No. 57704, QIAGEN, Hilden, Germany). .. The cDNA synthesis and first-round PCR steps were performed in a single reaction using the Superscript III One-Step RT PCR Platinum Taq HiFi kit (Cat. No. 12574035, Thermo Fisher, Massachusetts, USA). ..

    Article Title: Attenuation of Chikungunya Virus by a Single Amino Acid Substitution in the nsP1 Component of a Non-Structural Polyprotein.
    Article Snippet: .. The genome sequence was determined using an Applied Biosystems 3730 DNA analyser from overlapping cDNA fragments amplified by RT-PCR using a Superscript III One-step RT-PCR Platinum® Taq HiFi kit (ThermoFisher Scientific, Dartford, UK). .. Manipulation and analysis of the nucleotide sequence data obtained were undertaken using the Lasergene suite of programs (DNAStar, version 17.2).

    Article Title: A viral protein targets mitochondria and chloroplasts by subverting general import pathways and specific receptors.
    Article Snippet: .. The corresponding cDNAs were RT-PCR amplified using SuperScript III One-Step RT-PCR Platinum Taq HiFi following the supplier protocol (Thermo Fisher Scientific, Carlsbad, CA, USA), and N. benthamiana RNAs. ..

    Nested PCR:

    Article Title: Patterns of HIV-1 Drug Resistance Observed Through Geospatial Analysis of Routine Diagnostic Testing in KwaZulu-Natal, South Africa
    Article Snippet: We performed reverse transcription and nested polymerase chain reaction (PCR) amplification of the protease (PR) and reverse transcriptase (RT) genes using the Applied Biosystems HIV-1 Genotyping Kit (ThermoFisher Scientific, Waltham, MA, USA). .. We reversed transcribed and amplified the integrase (IN) gene using Superscript III one-step RT-PCR system with HiFi Platinum Taq (ThermoFisher Scientific, Waltham, MA, USA) and conducted nested PCR using the Expand High Fidelity Plus PCR System (Merck Lifesciences, Rockville, MD, USA). ..

    Polymerase Chain Reaction:

    Article Title: Patterns of HIV-1 Drug Resistance Observed Through Geospatial Analysis of Routine Diagnostic Testing in KwaZulu-Natal, South Africa
    Article Snippet: We performed reverse transcription and nested polymerase chain reaction (PCR) amplification of the protease (PR) and reverse transcriptase (RT) genes using the Applied Biosystems HIV-1 Genotyping Kit (ThermoFisher Scientific, Waltham, MA, USA). .. We reversed transcribed and amplified the integrase (IN) gene using Superscript III one-step RT-PCR system with HiFi Platinum Taq (ThermoFisher Scientific, Waltham, MA, USA) and conducted nested PCR using the Expand High Fidelity Plus PCR System (Merck Lifesciences, Rockville, MD, USA). ..

    Article Title: Unique molecular signatures in rebound viruses from antiretroviral drug and CRISPR-treated HIV-1-infected humanized mice
    Article Snippet: RNA extraction was performed on 50 μL of plasma collected in EDTA blood collection tubes using a Qiagen MinElute virus spin kit (Cat. No. 57704, QIAGEN, Hilden, Germany). .. The cDNA synthesis and first-round PCR steps were performed in a single reaction using the Superscript III One-Step RT PCR Platinum Taq HiFi kit (Cat. No. 12574035, Thermo Fisher, Massachusetts, USA). ..

    cDNA Synthesis:

    Article Title: Unique molecular signatures in rebound viruses from antiretroviral drug and CRISPR-treated HIV-1-infected humanized mice
    Article Snippet: RNA extraction was performed on 50 μL of plasma collected in EDTA blood collection tubes using a Qiagen MinElute virus spin kit (Cat. No. 57704, QIAGEN, Hilden, Germany). .. The cDNA synthesis and first-round PCR steps were performed in a single reaction using the Superscript III One-Step RT PCR Platinum Taq HiFi kit (Cat. No. 12574035, Thermo Fisher, Massachusetts, USA). ..

    Sequencing:

    Article Title: Attenuation of Chikungunya Virus by a Single Amino Acid Substitution in the nsP1 Component of a Non-Structural Polyprotein.
    Article Snippet: .. The genome sequence was determined using an Applied Biosystems 3730 DNA analyser from overlapping cDNA fragments amplified by RT-PCR using a Superscript III One-step RT-PCR Platinum® Taq HiFi kit (ThermoFisher Scientific, Dartford, UK). .. Manipulation and analysis of the nucleotide sequence data obtained were undertaken using the Lasergene suite of programs (DNAStar, version 17.2).



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