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superscript ii rnase h reverse transcriptase kit  (Thermo Fisher)


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    Thermo Fisher superscript ii rnase h reverse transcriptase kit
    Superscript Ii Rnase H Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+rnase+h+reverse+transcriptase+kit/Ribonuclease+A/pm40930097-44-32-39
    Average 99 stars, based on 1 article reviews
    superscript ii rnase h reverse transcriptase kit - by Bioz Stars, 2026-10
    99/100 stars

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    Immunoprecipitation:

    Article Title: RNA-binding proteins TDP-43 and FUS promote R-loop resolution and regulate transcription termination
    Article Snippet: .. RNase-sensitivity analysis for R-loops was carried out by adding 50 U of RNase H (Invitrogen, 18021–014) in 10X RNase H buffer (75 mM KCl, 50 mM Tris pH8.3, 3 mM MgCl2, 10 mM DTT) with 4% glycerol and 20 μg/ml BSA prior to immunoprecipitation; the RNase H treatment was performed for 2 h at 37 °C. ..

    Spectrophotometry:

    Article Title: Phenotypic and genotypic assessment of antimicrobial and biocide resistance in enterobacteria from anaerobic digestion of dairy waste: implications for environmental and public health
    Article Snippet: Genomic DNA was then extracted from the resulting bacterial cultures using a commercial kit, following the manufacturer’s instructions (PureLink Genomic DNA Mini Kit, Invitrogen). .. The DNA was quantified using a Nanodrop spectrophotometer (Thermo Scientific) and diluted to a concentration of 10 ng/μL in ultrapure, RNase- and DNase-free water (Invitrogen). ..

    Concentration Assay:

    Article Title: Phenotypic and genotypic assessment of antimicrobial and biocide resistance in enterobacteria from anaerobic digestion of dairy waste: implications for environmental and public health
    Article Snippet: Genomic DNA was then extracted from the resulting bacterial cultures using a commercial kit, following the manufacturer’s instructions (PureLink Genomic DNA Mini Kit, Invitrogen). .. The DNA was quantified using a Nanodrop spectrophotometer (Thermo Scientific) and diluted to a concentration of 10 ng/μL in ultrapure, RNase- and DNase-free water (Invitrogen). ..

    Incubation:

    Article Title: Therapeutic implications of targeting cancer testis antigen MAGEA1 in cervical cancer
    Article Snippet: .. RNase A (50 μ l; 100 μ g/ml; Thermo Fisher Scientific, Inc.) was added and incubated at 37°C water bath for 20 min. Then, the cells were treated with 5 μ l propidium iodide (10 mg/ml, Invitrogen; Thermo Fisher Scientific, Inc.) seeded in 96 well plates with density at 1×10 6 cells/well and incubated at 4°C in dark until flow cytometry readings. .. Cell cycle distribution was measured by flow cytometry (CytoFLEX V0-B3-R2 Flow Cytometer; Beckman Coulter, Inc.).

    Flow Cytometry:

    Article Title: Therapeutic implications of targeting cancer testis antigen MAGEA1 in cervical cancer
    Article Snippet: .. RNase A (50 μ l; 100 μ g/ml; Thermo Fisher Scientific, Inc.) was added and incubated at 37°C water bath for 20 min. Then, the cells were treated with 5 μ l propidium iodide (10 mg/ml, Invitrogen; Thermo Fisher Scientific, Inc.) seeded in 96 well plates with density at 1×10 6 cells/well and incubated at 4°C in dark until flow cytometry readings. .. Cell cycle distribution was measured by flow cytometry (CytoFLEX V0-B3-R2 Flow Cytometer; Beckman Coulter, Inc.).



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