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r1055 superscript iii reverse transcriptase thermofisher scientific  (Zymo Research)


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    Structured Review

    Zymo Research r1055 superscript iii reverse transcriptase thermofisher scientific
    R1055 Superscript Iii Reverse Transcriptase Thermofisher Scientific, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 4298 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+reverse+transcriptase+kit/Quick-RNA+Miniprep+Kit/pm40834857-157-62-58
    Average 99 stars, based on 4298 article reviews
    r1055 superscript iii reverse transcriptase thermofisher scientific - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Parasite:

    Article Title: Inducible dCas12a system expands CRISPRi/a capabilities for functional genomics in malaria parasite Plasmodium falciparum
    Article Snippet: Western blot was done with goat anti-GFP (1:2000, Novus Biologicals), anti-FLAG (1:2000, Abcam), anti-LbCas12a (1:500, Sigma-Aldrich), and rabbit anti-PfAldolase (1:2000, kindly provided by Dr. Tobias Spielmann) antibodies as primary antibodies, followed by HRP-conjugated donkey anti-goat IgG and goat anti-rabbit IgG (Sigma) as secondary antibodies. .. Parasites were released from infected RBCs with 0.1% (v/v) saponin, and total RNA was extracted using the Quick-RNA MiniPrep kit (Zymo Research). .. cDNA synthesis was performed using SuperScript IV RT (Invitrogen), and qPCR with FastStart Universal SYBR Green Master Mix (Roche).

    Infection:

    Article Title: Inducible dCas12a system expands CRISPRi/a capabilities for functional genomics in malaria parasite Plasmodium falciparum
    Article Snippet: Western blot was done with goat anti-GFP (1:2000, Novus Biologicals), anti-FLAG (1:2000, Abcam), anti-LbCas12a (1:500, Sigma-Aldrich), and rabbit anti-PfAldolase (1:2000, kindly provided by Dr. Tobias Spielmann) antibodies as primary antibodies, followed by HRP-conjugated donkey anti-goat IgG and goat anti-rabbit IgG (Sigma) as secondary antibodies. .. Parasites were released from infected RBCs with 0.1% (v/v) saponin, and total RNA was extracted using the Quick-RNA MiniPrep kit (Zymo Research). .. cDNA synthesis was performed using SuperScript IV RT (Invitrogen), and qPCR with FastStart Universal SYBR Green Master Mix (Roche).

    RNA Extraction:

    Article Title: Single-gRNA CRISPR/eCas9-mediated simultaneous targeting of cIAP1 and cIAP2 in colorectal cancer cells: RNA-seq reveals distinct transcriptomic and apoptotic responses.
    Article Snippet: Cellular inhibitor of apoptosis proteins 1 and 2 (cIAP1 and cIAP2), encoded by BIRC2 and BIRC3, respectively, are important regulators of cellular survival and apoptosis.. These proteins contribute to cancer cell survival by suppressing apoptotic signaling.. While individual knockouts of BIRC2 or BIRC3 in cancer cells have been investigated, the consequences of simultaneously targeting both paralogs remain poorly understood.

    Control:

    Article Title: Single-gRNA CRISPR/eCas9-mediated simultaneous targeting of cIAP1 and cIAP2 in colorectal cancer cells: RNA-seq reveals distinct transcriptomic and apoptotic responses.
    Article Snippet: Cellular inhibitor of apoptosis proteins 1 and 2 (cIAP1 and cIAP2), encoded by BIRC2 and BIRC3, respectively, are important regulators of cellular survival and apoptosis.. These proteins contribute to cancer cell survival by suppressing apoptotic signaling.. While individual knockouts of BIRC2 or BIRC3 in cancer cells have been investigated, the consequences of simultaneously targeting both paralogs remain poorly understood.

    Extraction:

    Article Title: Single-gRNA CRISPR/eCas9-mediated simultaneous targeting of cIAP1 and cIAP2 in colorectal cancer cells: RNA-seq reveals distinct transcriptomic and apoptotic responses.
    Article Snippet: Cellular inhibitor of apoptosis proteins 1 and 2 (cIAP1 and cIAP2), encoded by BIRC2 and BIRC3, respectively, are important regulators of cellular survival and apoptosis.. These proteins contribute to cancer cell survival by suppressing apoptotic signaling.. While individual knockouts of BIRC2 or BIRC3 in cancer cells have been investigated, the consequences of simultaneously targeting both paralogs remain poorly understood.

    Isolation:

    Article Title: Ciprofloxacin-Induced Oxidative Stress Promotes a Senescence-like Phenotype in Human Fibroblasts In Vitro.
    Article Snippet: .. Total cellular RNA was isolated using the Quick-RNA MiniPrep kit (Zymo Research, Irvine, CA, USA) according to the manufacturer’s protocol. .. RNA concentration was determined with a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, USA), and RNA quality was verified by electrophoresis.

    Article Title: Programmable and selective silencing of oncogenic fusion transcripts by CRISPR-Cas13b via breakpoint sequence recognition
    Article Snippet: .. Total RNA was isolated from ∼0.2 to 1 × 10 6 cells using the NucleoSpin RNA Plus (Macherey-Nagel, 740984.50) or Quick - RNA Miniprep Kit (Zymo Research, R1055) following the manufacturer’s instructions. .. Alternatively, total RNA was isolated by standard TRIzol-chloroform extraction according to the manufacturer’s instruction (Thermo Fisher Scientific, 15596026), followed by deoxyribonuclease (Dnase) treatment with RQ1 RNase-Free DNase according to the manufacturer’s instruction (Promega, M6101).

    cDNA Synthesis:

    Article Title: Exposure and Bioaccumulation of Mercury Impacted the Extent of Cellular Damage and Oxidative Stress in Local Chickens Around Ilesha Gold Mining Areas of Nigeria.
    Article Snippet: Mercury (Hg) is a wellknown global pollutant of environmental concern.. Uncontrolled mining activities represent an avenue for its release and possible environmental contamination.. Ilesha gold mining areas are characterized by extensive artisan and smallscale gold mining (ASGM) activities.



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