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Bioteke Corporation super m mlv reverse transcriptase
Super M Mlv Reverse Transcriptase, supplied by Bioteke Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+m-mlv+reverse+transcriptase+pr6502/m+mlv+reverse+super+transcriptase/pmc12440323-40-31-38
Average 86 stars, based on 1 article reviews
super m mlv reverse transcriptase - by Bioz Stars, 2026-10
86/100 stars

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Reverse Transcription:

Article Title: Circular RNA hsa_circ_0001666 sponges miR‑330‑5p, miR‑193a‑5p and miR‑326, and promotes papillary thyroid carcinoma progression via upregulation of ETV4.
Article Snippet: .. Total RNA from all PTC cell lines, clinical tumor, adjacent normal or xenograft tumor tissues were extracted using TRIpure (BioTeke Corporation) and reverse‐transcribed into cDNA using a Super M‐MLV Reverse Transcriptase kit (BioTeke Corporation) according to the manufacturer's procedure at 42 ̊C for 50 min. RT‐qPCR was performed using SYBR Green (Sigma‐Aldrich; Merck KGaA). ..

Article Title: Long Non-Coding RNA NR_027324 Protects H9C2 Cells against Hypoglycemic and Hypoxic Injury by Interacting with microRNA-103-3p.
Article Snippet: The absorbance was measured at 490 nm using an enzyme-linked immunosorbent assay (ELX-800; Biotek), and each group was replicated 3 times. qRT-PCR: Total RNA was extracted using TRIpure reagent (#RP1001; BioTeke, Beijing, China). .. The concentration and purity of RNA were measured using a Nanodrop 2000 system (ThermoFisher Scientific). cDNAs were synthesized using Super M-MLV reverse transcriptase (#PR 6502; BioTeke) following the manufacturer’s protocol. .. SYBR green master mix (#SY1020; Solarbio Technology, Beijing, China) was used to amplify the cDNA samples.

Article Title: Schisantherin A alleviated alcohol-induced liver injury by the regulation of alcohol metabolism and NF-kB pathway.
Article Snippet: The activities of aspartate aminotransferase (aST) and alanine transaminase (aLT) in serum were determined by commercial kits (nanjing Jiancheng Bioengineering institute, Jiangsu, China). all detection procedures were strictly performed in accordance with the manufacturer’s instructions. .. Total Rna was extracted by using the TRizol solution (Bioteke Corporation, Beijing, China). cDna was synthesized from total Rna with Super M-MLV Reverse Transcriptase (Bioteke Corporation). .. The primer sequences used in this study were as follows: 5’-ctggagatctaccgatacaca-3’, forward, and 5’-gcagcaggatggctaagaag-3’, reverse, for CYP1a2; 5’-ccaccctcctcctcgtatc-3’, forward, and 5’-ccttgacagccttgtagcc-3’, reverse, for CYP2e1; and 5’-ctgtgcccatctacgagggctat-3’, forward, and 5’-tttgatgtcacgcacgatttcc-3’, reverse, for β-actin. qPCR was performed with 2×Power Taq PCR MasterMix (Bioteke Corporation) and SYBR green (Solarbio, Beijing, China) on an exicycler TM 96 (Bioneer, Daejeon, Korea).

Article Title: Calcium and integrin binding protein 1 (CIB1) induces myocardial fibrosis in myocardial infarction via regulating the PI3K/Akt pathway.
Article Snippet: The total Rna isolation reagent TRipure (Bioteke, Beijing, China) was used to extract Rna in tissues or cells. .. Then Rna was reverse-transcribed by Super MMLV reverse transcriptase (Bioteke), and cDna was amplificated with 2×Taq PCR MasterMix (Solarbio, Beijing, China) and detected on ExicyclerTM 96 Realtime PCR System (Bioneer Corporation, Daejeon, Korea). .. The primers were synthesized by Genscript Biotechnology Co., Ltd. (nanjing, China) and listed in Table 1.

Article Title: CALCOCO2 silencing represents a potential molecular therapeutic target for glioma
Article Snippet: Briefly, total messenger RNAs (mRNAs) of cells were extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). .. The mature mRNAs (2 μg per sample) were reverse transcribed into cDNAs using Super MMLV reverse transcriptase (BioTeke, Beijing, China). .. The mRNA levels of CALCOCO2 were determined by RT-qPCR using the Bio-Rad Connect Real-time PCR platform.

Article Title: Prevention of the Progression of lupus Nephritis in MRL/lpr Mice by Modulating miR-9-5p/Foxo1 Axis
Article Snippet: .. Total RNA in cells or tissue samples was extracted using the Trizol technique (Thermo, USA) and RNA concentration was evaluated using NANO 2000. cDNA templates were produced with the help of Super M-MLV Reverse Transcriptase (cat. no. PR6502, BioTeke, China). .. Real-time PCR was then performed on cDNA templates using a reaction system of 20 μl, 1 μl template, 0.5 μl of each primer (miR-9-5p, forward: 5′-TGCGCTCTTTGGTTATCTAGCTG-3′, reverse, 5′-CCAGTGCAGGGTCCGAGGTATT-3′; U6, forward: 5′-CGCAAGGATGACACGCAAAT-3′, reverse: 5′-GTGCAGGGTCCGAGGTATTC-3′), 10 μl 2× Power Taq PCR MasterMix (BioTeke, China), and 8 μl ddH 2 O.

Article Title: Prevention of the Progression of lupus Nephritis in MRL/lpr Mice by Modulating miR-9-5p/Foxo1 Axis
Article Snippet: .. Total RNA in cells or tissue samples was extracted using the Trizol technique (Thermo, USA) and RNA concentration was evaluated using NANO 2000. cDNA templates were produced with the help of Super M-MLV Reverse Transcriptase (cat. no. PR6502, BioTeke, China). .. Real-time PCR was then performed on cDNA templates using a reaction system of 20 μl, 1 μl template, 0.5 μl of each primer (miR-9-5p, forward: 5’-TGCGCTCTTTGGTTATCTAGCTG-3’, reverse, 5’-CCAGTGCAGGGTCCGAGGTATT-3’; U6, forward: 5’-CGCAAGGATGACACGCAAAT-3’, reverse: 5’-GTGCAGGGTCCGAGGTATTC-3’), 10 μl 2× Power Taq PCR MasterMix (BioTeke, China), and 8 μl ddH2O.

SYBR Green Assay:

Article Title: Circular RNA hsa_circ_0001666 sponges miR‑330‑5p, miR‑193a‑5p and miR‑326, and promotes papillary thyroid carcinoma progression via upregulation of ETV4.
Article Snippet: .. Total RNA from all PTC cell lines, clinical tumor, adjacent normal or xenograft tumor tissues were extracted using TRIpure (BioTeke Corporation) and reverse‐transcribed into cDNA using a Super M‐MLV Reverse Transcriptase kit (BioTeke Corporation) according to the manufacturer's procedure at 42 ̊C for 50 min. RT‐qPCR was performed using SYBR Green (Sigma‐Aldrich; Merck KGaA). ..

Concentration Assay:

Article Title: Long Non-Coding RNA NR_027324 Protects H9C2 Cells against Hypoglycemic and Hypoxic Injury by Interacting with microRNA-103-3p.
Article Snippet: The absorbance was measured at 490 nm using an enzyme-linked immunosorbent assay (ELX-800; Biotek), and each group was replicated 3 times. qRT-PCR: Total RNA was extracted using TRIpure reagent (#RP1001; BioTeke, Beijing, China). .. The concentration and purity of RNA were measured using a Nanodrop 2000 system (ThermoFisher Scientific). cDNAs were synthesized using Super M-MLV reverse transcriptase (#PR 6502; BioTeke) following the manufacturer’s protocol. .. SYBR green master mix (#SY1020; Solarbio Technology, Beijing, China) was used to amplify the cDNA samples.

Article Title: Prevention of the Progression of lupus Nephritis in MRL/lpr Mice by Modulating miR-9-5p/Foxo1 Axis
Article Snippet: .. Total RNA in cells or tissue samples was extracted using the Trizol technique (Thermo, USA) and RNA concentration was evaluated using NANO 2000. cDNA templates were produced with the help of Super M-MLV Reverse Transcriptase (cat. no. PR6502, BioTeke, China). .. Real-time PCR was then performed on cDNA templates using a reaction system of 20 μl, 1 μl template, 0.5 μl of each primer (miR-9-5p, forward: 5′-TGCGCTCTTTGGTTATCTAGCTG-3′, reverse, 5′-CCAGTGCAGGGTCCGAGGTATT-3′; U6, forward: 5′-CGCAAGGATGACACGCAAAT-3′, reverse: 5′-GTGCAGGGTCCGAGGTATTC-3′), 10 μl 2× Power Taq PCR MasterMix (BioTeke, China), and 8 μl ddH 2 O.

Article Title: Prevention of the Progression of lupus Nephritis in MRL/lpr Mice by Modulating miR-9-5p/Foxo1 Axis
Article Snippet: .. Total RNA in cells or tissue samples was extracted using the Trizol technique (Thermo, USA) and RNA concentration was evaluated using NANO 2000. cDNA templates were produced with the help of Super M-MLV Reverse Transcriptase (cat. no. PR6502, BioTeke, China). .. Real-time PCR was then performed on cDNA templates using a reaction system of 20 μl, 1 μl template, 0.5 μl of each primer (miR-9-5p, forward: 5’-TGCGCTCTTTGGTTATCTAGCTG-3’, reverse, 5’-CCAGTGCAGGGTCCGAGGTATT-3’; U6, forward: 5’-CGCAAGGATGACACGCAAAT-3’, reverse: 5’-GTGCAGGGTCCGAGGTATTC-3’), 10 μl 2× Power Taq PCR MasterMix (BioTeke, China), and 8 μl ddH2O.

Synthesized:

Article Title: Long Non-Coding RNA NR_027324 Protects H9C2 Cells against Hypoglycemic and Hypoxic Injury by Interacting with microRNA-103-3p.
Article Snippet: The absorbance was measured at 490 nm using an enzyme-linked immunosorbent assay (ELX-800; Biotek), and each group was replicated 3 times. qRT-PCR: Total RNA was extracted using TRIpure reagent (#RP1001; BioTeke, Beijing, China). .. The concentration and purity of RNA were measured using a Nanodrop 2000 system (ThermoFisher Scientific). cDNAs were synthesized using Super M-MLV reverse transcriptase (#PR 6502; BioTeke) following the manufacturer’s protocol. .. SYBR green master mix (#SY1020; Solarbio Technology, Beijing, China) was used to amplify the cDNA samples.

Article Title: Schisantherin A alleviated alcohol-induced liver injury by the regulation of alcohol metabolism and NF-kB pathway.
Article Snippet: The activities of aspartate aminotransferase (aST) and alanine transaminase (aLT) in serum were determined by commercial kits (nanjing Jiancheng Bioengineering institute, Jiangsu, China). all detection procedures were strictly performed in accordance with the manufacturer’s instructions. .. Total Rna was extracted by using the TRizol solution (Bioteke Corporation, Beijing, China). cDna was synthesized from total Rna with Super M-MLV Reverse Transcriptase (Bioteke Corporation). .. The primer sequences used in this study were as follows: 5’-ctggagatctaccgatacaca-3’, forward, and 5’-gcagcaggatggctaagaag-3’, reverse, for CYP1a2; 5’-ccaccctcctcctcgtatc-3’, forward, and 5’-ccttgacagccttgtagcc-3’, reverse, for CYP2e1; and 5’-ctgtgcccatctacgagggctat-3’, forward, and 5’-tttgatgtcacgcacgatttcc-3’, reverse, for β-actin. qPCR was performed with 2×Power Taq PCR MasterMix (Bioteke Corporation) and SYBR green (Solarbio, Beijing, China) on an exicycler TM 96 (Bioneer, Daejeon, Korea).

Polymerase Chain Reaction:

Article Title: Calcium and integrin binding protein 1 (CIB1) induces myocardial fibrosis in myocardial infarction via regulating the PI3K/Akt pathway.
Article Snippet: The total Rna isolation reagent TRipure (Bioteke, Beijing, China) was used to extract Rna in tissues or cells. .. Then Rna was reverse-transcribed by Super MMLV reverse transcriptase (Bioteke), and cDna was amplificated with 2×Taq PCR MasterMix (Solarbio, Beijing, China) and detected on ExicyclerTM 96 Realtime PCR System (Bioneer Corporation, Daejeon, Korea). .. The primers were synthesized by Genscript Biotechnology Co., Ltd. (nanjing, China) and listed in Table 1.

Produced:

Article Title: Prevention of the Progression of lupus Nephritis in MRL/lpr Mice by Modulating miR-9-5p/Foxo1 Axis
Article Snippet: .. Total RNA in cells or tissue samples was extracted using the Trizol technique (Thermo, USA) and RNA concentration was evaluated using NANO 2000. cDNA templates were produced with the help of Super M-MLV Reverse Transcriptase (cat. no. PR6502, BioTeke, China). .. Real-time PCR was then performed on cDNA templates using a reaction system of 20 μl, 1 μl template, 0.5 μl of each primer (miR-9-5p, forward: 5′-TGCGCTCTTTGGTTATCTAGCTG-3′, reverse, 5′-CCAGTGCAGGGTCCGAGGTATT-3′; U6, forward: 5′-CGCAAGGATGACACGCAAAT-3′, reverse: 5′-GTGCAGGGTCCGAGGTATTC-3′), 10 μl 2× Power Taq PCR MasterMix (BioTeke, China), and 8 μl ddH 2 O.

Article Title: Prevention of the Progression of lupus Nephritis in MRL/lpr Mice by Modulating miR-9-5p/Foxo1 Axis
Article Snippet: .. Total RNA in cells or tissue samples was extracted using the Trizol technique (Thermo, USA) and RNA concentration was evaluated using NANO 2000. cDNA templates were produced with the help of Super M-MLV Reverse Transcriptase (cat. no. PR6502, BioTeke, China). .. Real-time PCR was then performed on cDNA templates using a reaction system of 20 μl, 1 μl template, 0.5 μl of each primer (miR-9-5p, forward: 5’-TGCGCTCTTTGGTTATCTAGCTG-3’, reverse, 5’-CCAGTGCAGGGTCCGAGGTATT-3’; U6, forward: 5’-CGCAAGGATGACACGCAAAT-3’, reverse: 5’-GTGCAGGGTCCGAGGTATTC-3’), 10 μl 2× Power Taq PCR MasterMix (BioTeke, China), and 8 μl ddH2O.



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Bioteke Corporation superscript m-mlv reverse transcriptase pr6502
Superscript M Mlv Reverse Transcriptase Pr6502, supplied by Bioteke Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+m-mlv+reverse+transcriptase+pr6502/super+m+mlv+reverse+transcriptase+pr6502/pm34888748-51-5-10
Average 90 stars, based on 1 article reviews
superscript m-mlv reverse transcriptase pr6502 - by Bioz Stars, 2026-10
90/100 stars
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