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vilo superscript iv reverse transcriptase kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher vilo superscript iv reverse transcriptase kit
    Vilo Superscript Iv Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iv+vilo+reverse+transcriptase+kit/pm37979585-617-28-34
    Average 86 stars, based on 1 article reviews
    vilo superscript iv reverse transcriptase kit - by Bioz Stars, 2026-09
    86/100 stars

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    other:

    Article Title: Hamsters with long Covid exhibits a neurodegenerative signature in the brainstem
    Article Snippet: Briefly, RNA was reverse transcribed to first strand cDNA using the SuperScriptTM IV VILOTM Master Mix (11766050, Invitrogen). qPCR was performed in a final volume of 10LJμL per reaction in 384-well PCR plates using a thermocycler (QuantStudio 6 Flex, Applied Biosystems) and its related software (QuantStudio Real-time PCR System, Design and Analysis Software v. 2.7.0, Applied Biosystems).

    Article Title: The SARS-CoV-2 envelope PDZ Binding Motif acts as a virulence factor disrupting host’s epithelial cell-cell junctions
    Article Snippet: RNA was reverse transcribed into cDNA using SuperScriptTM IV VILOTM Master Mix (11766050, Invitrogen ). qPCR was performed according to the manufacturer’s protocols for either TaqMan Fast Advanced Master Mix (444457, Applied Biosystems ) or SYBR Green Master Mix (4309155, Applied Biosystems ), using 2.5 μL of cDNA (12.5 ng) and golden hamster primer pairs for the genes of interest, as reported in the literature , .

    Polymerase Chain Reaction:

    Article Title: RNA splicing variants of the novel long non-coding RNA, CyKILR, possess divergent biological functions in non-small cell lung cancer
    Article Snippet: RNA Subcellular Isolation Kit was from Active Motif (25501). .. The SuperScript IV VILO Master Mix with ezDNase Enzyme (11766050), TaqMan Universal PCR Master Mix (4324018), Opti-MEM I Reduced Serum Medium (31985062), Lipofectamine RNAiMAX Transfection Reagent (13778150), and Pierce Fast Western Blot Kit (35050) were from Thermo Fisher Scientific. .. The Cell Titer-Glo 2.0 Assay Kit was purchased from Promega (G9242).

    Transfection:

    Article Title: RNA splicing variants of the novel long non-coding RNA, CyKILR, possess divergent biological functions in non-small cell lung cancer
    Article Snippet: RNA Subcellular Isolation Kit was from Active Motif (25501). .. The SuperScript IV VILO Master Mix with ezDNase Enzyme (11766050), TaqMan Universal PCR Master Mix (4324018), Opti-MEM I Reduced Serum Medium (31985062), Lipofectamine RNAiMAX Transfection Reagent (13778150), and Pierce Fast Western Blot Kit (35050) were from Thermo Fisher Scientific. .. The Cell Titer-Glo 2.0 Assay Kit was purchased from Promega (G9242).

    Western Blot:

    Article Title: RNA splicing variants of the novel long non-coding RNA, CyKILR, possess divergent biological functions in non-small cell lung cancer
    Article Snippet: RNA Subcellular Isolation Kit was from Active Motif (25501). .. The SuperScript IV VILO Master Mix with ezDNase Enzyme (11766050), TaqMan Universal PCR Master Mix (4324018), Opti-MEM I Reduced Serum Medium (31985062), Lipofectamine RNAiMAX Transfection Reagent (13778150), and Pierce Fast Western Blot Kit (35050) were from Thermo Fisher Scientific. .. The Cell Titer-Glo 2.0 Assay Kit was purchased from Promega (G9242).

    Reverse Transcription:

    Article Title: Age-dependent regulation of axoglial interactions and behavior by oligodendrocyte AnkyrinG
    Article Snippet: .. Briefly, IP and input RNA samples were reverse transcribed into cDNA using SuperScript IV VILO MasterMix, followed by genomic DNA removal with ezDNase Enzyme (Invitrogen Cat# 11766050). .. And quantitative PCR (qPCR) was performed using iTaq Universal SYBR Green Supermix (Bio-Rad Cat# 1725122) using the StepOnePlus Real-Time PCR System.

    Article Title: Exploring the Role of Kv1.3 and MAPK14 in Mediating Microglial Oxidative Stress and Neuroinflammation Following Organic Dust Exposure
    Article Snippet: .. 1 μg of RNA was reverse transcribed into cDNA using the superscript IV VILO Kit (Thermo Fisher Scientific, cat. # 11766050) following the manufacturer’s protocol. .. For qPCR, 5 μL of PowerUpTM SYBR Green Master mix (Thermo Fisher Scientific, cat. # 25742), 0.5 μL each of forward and reverse primers (10 μM), 3 μL of water and 1 μL of cDNA (1-10 ng) were used.

    Synthesized:

    Article Title: Dietary Carrageenan Amplifies the Inflammatory Profile, but not Permeability, of Intestinal Epithelial Cells from Patients With Crohn's Disease.
    Article Snippet: *Department of Chronic Diseases and Metabolism (CHROMETA), Translational Research Center for Gastrointestinal Disorders (TARGID), KU Leuven, Herestraat 49, 3000 Leuven, Belgium †Department of Gastroenterology and Hepatology, UZ Leuven, Herestraat 49, 3000 Leuven, Belgium ‡Department of Microbiology, Immunology and Transplantation, Nephrology and Renal Transplantation Research Group, KU Leuven, Herestraat 49, 3000 Leuven, Belgium §Department of Chronic Diseases and Metabolism, Clinical and Experimental Endocrinology, KU Leuven, Herestraat 49, 3000 Leuven, Belgium ¶Department of Endocrinology, UZ Leuven, Herestraat 49, 3000 Leuven, Belgium ‖K.A. and J.S. shared the last authorship.. Address correspondence to: João Sabino, MD, PhD, Deparment of Gastroenterology and Hepatology, UZ Leuven, Herestraat 49, 3000 Leuven, Belgium.. Tel.

    Article Title: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications
    Article Snippet: DNA digestion was performed using the Invitrogen ezDNase enzyme (Thermo Fisher Scientific, cat# 11766051). .. Complementary DNA (cDNA) was then synthesized from 10 ng RNA using the Invitrogen SuperScript IV VILO Master Mix kit (Thermo Fisher Scientific, cat# 11766050) or Invitrogen SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific, cat# 11754050) following the manufacturer protocol. .. Sequencing libraries were prepared from the total cDNA synthesis product using the Ion Torrent Ion AmpliSeq Transcriptome Human Gene Expression Panel, Chef-Ready Kit (Thermo Fisher Scientific, cat# A31446) and the Ion Chef instrument model 4247 following manufacturer’s instructions (MAN0010742 (Revision C.0)) and using 12–13, 16-minute amplification cycles.

    Purification:

    Article Title: Malaria parasites require a divergent heme oxygenase for apicoplast gene expression and biogenesis
    Article Snippet: Parasite DNA was extracted with a QIAamp DNA Blood Mini kit (QIAGEN, 51106), and RNA was purified by Trizol (Invitrogen, 15596026) and phenol-chloroform isolation. .. We converted 1 μg of purified RNA to cDNA using a SuperScript IV VILO RT kit (Invitrogen, 11766050). ..

    cDNA Synthesis:

    Article Title: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications
    Article Snippet: DNA digestion was performed using the Invitrogen ezDNase enzyme (Thermo Fisher Scientific, cat# 11766051). .. Complementary DNA (cDNA) was then synthesized from 10 ng RNA using the Invitrogen SuperScript IV VILO Master Mix kit (Thermo Fisher Scientific, cat# 11766050) or Invitrogen SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific, cat# 11754050) following the manufacturer protocol. .. Sequencing libraries were prepared from the total cDNA synthesis product using the Ion Torrent Ion AmpliSeq Transcriptome Human Gene Expression Panel, Chef-Ready Kit (Thermo Fisher Scientific, cat# A31446) and the Ion Chef instrument model 4247 following manufacturer’s instructions (MAN0010742 (Revision C.0)) and using 12–13, 16-minute amplification cycles.



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